a pleuropneumoniae serotype 1  (ATCC)


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    ATCC a pleuropneumoniae serotype 1
    2-D DIGE of
    A Pleuropneumoniae Serotype 1, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Proteomic and immunoproteomic characterization of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae"

    Article Title: Proteomic and immunoproteomic characterization of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-23

    2-D DIGE of
    Figure Legend Snippet: 2-D DIGE of "detergent-wash" proteins from A. pleuropneumoniae serotypes 1, 2 and 5 (subunit vaccine) and serotype 7 . For analysis of the subunit vaccine serotype 1, 2, and 5 were labelled with Cy2 (shown in blue), Cy3 (shown in green) and Cy5 (shown in red), respectively, and subsequently pooled. For visualization of differences between the serotypes used for vaccine generation, we compared couples of serotypes 1 and 2 (A), serotypes 1 and 5 (B), and serotypes 2 and 5 (C). For comparison of the subunit vaccine to serotype 7, the subunit vaccine was labelled with Cy3 (shown in green) and the "detergent wash" of serotype 7 was labelled with Cy5 (shown in red, D). Spots with intensities considerably above that in one or the other serotype were analyzed by mass spectrometry from preparative gels of the respective serotype (Additional file , Figure S2). The annotation of the identified protein is given in the same colour as the labelling of the respective serotype. The numbers on each spot, that has been identified, are consecutive and allow the finding of the respective spot on preparative gels (Additional file , Figure S2).

    Techniques Used: Mass Spectrometry

    actinobacillus pleuropneumoniae  (ATCC)


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    Structured Review

    ATCC actinobacillus pleuropneumoniae
    Number of <t> Actinobacillus pleuropneumoniae </t> (APP), Pasteurella multocida , and Escherichia coli isolates isolated during the study period (2019–2022). The number of samples submitted per year is also provided in brackets to identify the suspected pathogen.
    Actinobacillus Pleuropneumoniae, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The Susceptibility Trends of Respiratory and Enteric Porcine Pathogens to Last-Resource Antimicrobials"

    Article Title: The Susceptibility Trends of Respiratory and Enteric Porcine Pathogens to Last-Resource Antimicrobials

    Journal: Antibiotics

    doi: 10.3390/antibiotics12111575

    Number of  Actinobacillus pleuropneumoniae  (APP), Pasteurella multocida , and Escherichia coli isolates isolated during the study period (2019–2022). The number of samples submitted per year is also provided in brackets to identify the suspected pathogen.
    Figure Legend Snippet: Number of Actinobacillus pleuropneumoniae (APP), Pasteurella multocida , and Escherichia coli isolates isolated during the study period (2019–2022). The number of samples submitted per year is also provided in brackets to identify the suspected pathogen.

    Techniques Used: Isolation

    Minimum inhibitory concentration (MIC) distribution values of (A)  Actinobacillus pleuropneumoniae  , (B) Pasteurella multocida , and (C) Escherichia coli to quinolones (enrofloxacin and marbofloxacin) from 2019 to 2022 in Spain. MIC range is the minimum and maximum MIC value observed. MIC90 and MIC50 values are the lowest concentration of the antibiotic at which 90 and 50% of the isolates were inhibited, respectively.
    Figure Legend Snippet: Minimum inhibitory concentration (MIC) distribution values of (A) Actinobacillus pleuropneumoniae , (B) Pasteurella multocida , and (C) Escherichia coli to quinolones (enrofloxacin and marbofloxacin) from 2019 to 2022 in Spain. MIC range is the minimum and maximum MIC value observed. MIC90 and MIC50 values are the lowest concentration of the antibiotic at which 90 and 50% of the isolates were inhibited, respectively.

    Techniques Used: Concentration Assay

    Minimum inhibitory concentration (MIC) distribution values of  Actinobacillus pleuropneumoniae  , Pasteurella multocida , and Escherichia coli to ceftiofur and cefquinome (only Esherichia coli ) from 2019 to 2022 in Spain. MIC range is the minimum and maximum MIC value observed. MIC90 and MIC50 values are the lowest concentration of the antibiotic at which 90 and 50% of the isolates were inhibited, respectively.
    Figure Legend Snippet: Minimum inhibitory concentration (MIC) distribution values of Actinobacillus pleuropneumoniae , Pasteurella multocida , and Escherichia coli to ceftiofur and cefquinome (only Esherichia coli ) from 2019 to 2022 in Spain. MIC range is the minimum and maximum MIC value observed. MIC90 and MIC50 values are the lowest concentration of the antibiotic at which 90 and 50% of the isolates were inhibited, respectively.

    Techniques Used: Concentration Assay

    Percentage of Actinobacillus pleuropneumoniae ( A ), Pasteurella multocida ( B ), and Escherichia coli ( C ), and isolates belonging to antimicrobial susceptibility category 1 (circle), category 2 (square), category 3 (diamond), and category 4 (triangle) for enrofloxacin. The number of categories for multinominal analysis was based on distributing the range of MIC values in four categories (from one to four), equally distributed, that include two MIC values per category, with category one being the most susceptible (lowest MIC value) and category four the least susceptible (highest MIC value).
    Figure Legend Snippet: Percentage of Actinobacillus pleuropneumoniae ( A ), Pasteurella multocida ( B ), and Escherichia coli ( C ), and isolates belonging to antimicrobial susceptibility category 1 (circle), category 2 (square), category 3 (diamond), and category 4 (triangle) for enrofloxacin. The number of categories for multinominal analysis was based on distributing the range of MIC values in four categories (from one to four), equally distributed, that include two MIC values per category, with category one being the most susceptible (lowest MIC value) and category four the least susceptible (highest MIC value).

    Techniques Used:

    Percentage of susceptible isolates by year for enrofloxacin ( A ) and marbofloxacin ( B ) of Actinobacillus pleuropneumoniae (circle), Pasteurella multocida (square), and Escherichia coli (diamond), using the CLSI and EUCAST clinical breakpoints, as detailed in the Materials and Methods section.
    Figure Legend Snippet: Percentage of susceptible isolates by year for enrofloxacin ( A ) and marbofloxacin ( B ) of Actinobacillus pleuropneumoniae (circle), Pasteurella multocida (square), and Escherichia coli (diamond), using the CLSI and EUCAST clinical breakpoints, as detailed in the Materials and Methods section.

    Techniques Used:

    The adjusted odds ratio (95% confidence interval) describing the annual variation in the susceptibility of  A. pleuropneumoniae  isolates to enrofloxacin using the logistic and multinominal regression model. The number of  A. pleuropneumoniae  isolates by year is detailed in <xref ref-type= Table 1 ." title="... describing the annual variation in the susceptibility of A. pleuropneumoniae isolates to enrofloxacin using the ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: The adjusted odds ratio (95% confidence interval) describing the annual variation in the susceptibility of A. pleuropneumoniae isolates to enrofloxacin using the logistic and multinominal regression model. The number of A. pleuropneumoniae isolates by year is detailed in Table 1 .

    Techniques Used:

    Percentage of Actinobacillus pleuropneumoniae ( A ), Pasteurella multocida ( B ), and Escherichia coli ( C ), and isolates belonging to antimicrobial susceptibility category 1 (circle), category 2 (square), category 3 (diamond), and category 4 (triangle) for marbofloxacin. The number of categories for multinominal analysis was based on distributing the range of MIC values in four categories (from one to four), equally distributed, that include two MIC values per category, with category one being the most susceptible (lowest MIC value) and category four the least susceptible (highest MIC value).
    Figure Legend Snippet: Percentage of Actinobacillus pleuropneumoniae ( A ), Pasteurella multocida ( B ), and Escherichia coli ( C ), and isolates belonging to antimicrobial susceptibility category 1 (circle), category 2 (square), category 3 (diamond), and category 4 (triangle) for marbofloxacin. The number of categories for multinominal analysis was based on distributing the range of MIC values in four categories (from one to four), equally distributed, that include two MIC values per category, with category one being the most susceptible (lowest MIC value) and category four the least susceptible (highest MIC value).

    Techniques Used:

    The adjusted odds ratio (95% confidence interval) describing the annual variation in the susceptibility of  A. pleuropneumoniae  isolates to marbofloxacin using the logistic and multinominal regression model. The number of  A. pleuropneumoniae  isolates by year is detailed in <xref ref-type= Table 1 ." title="... describing the annual variation in the susceptibility of A. pleuropneumoniae isolates to marbofloxacin using the ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: The adjusted odds ratio (95% confidence interval) describing the annual variation in the susceptibility of A. pleuropneumoniae isolates to marbofloxacin using the logistic and multinominal regression model. The number of A. pleuropneumoniae isolates by year is detailed in Table 1 .

    Techniques Used:

    Percentage of Actinobacillus pleuropneumoniae ( A ), Pasteurella multocida ( B ), and Escherichia coli ( C ) isolates belonging to antimicrobial susceptibility category 1 (circle), category 2 (square), category 3 (diamond), and category 4 (triangle) for ceftiofur. The number of categories for multinominal analysis was based on distributing the range of MIC values in four categories (from one to four), equally distributed, that include two MIC values per category, with category one being the most susceptible (lowest MIC value) and category four the least susceptible (highest MIC value).
    Figure Legend Snippet: Percentage of Actinobacillus pleuropneumoniae ( A ), Pasteurella multocida ( B ), and Escherichia coli ( C ) isolates belonging to antimicrobial susceptibility category 1 (circle), category 2 (square), category 3 (diamond), and category 4 (triangle) for ceftiofur. The number of categories for multinominal analysis was based on distributing the range of MIC values in four categories (from one to four), equally distributed, that include two MIC values per category, with category one being the most susceptible (lowest MIC value) and category four the least susceptible (highest MIC value).

    Techniques Used:

    Percentage of susceptible isolates by year for ceftiofur ( A ) of Actinobacillus pleuropneumoniae (circle), Pasteurella multocida (square), and Escherichia coli (diamond), as well as cefquinome ( B ) of Escherichia coli (diamond), using the CLSI and EUCAST clinical breakpoints, as detailed in the Materials and Methods section.
    Figure Legend Snippet: Percentage of susceptible isolates by year for ceftiofur ( A ) of Actinobacillus pleuropneumoniae (circle), Pasteurella multocida (square), and Escherichia coli (diamond), as well as cefquinome ( B ) of Escherichia coli (diamond), using the CLSI and EUCAST clinical breakpoints, as detailed in the Materials and Methods section.

    Techniques Used:

    Clinical breakpoints (susceptible/non-susceptible for each isolate) used according to the CLSI clinical breakpoints for  Actinobacillus pleuropneumoniae  (APP), Pasteurella multocida (PM), and Escherichia coli (EC) for quinolones and cephalosporins, and the EUCAST guidelines for colistin in the case of E. coli , respectively.
    Figure Legend Snippet: Clinical breakpoints (susceptible/non-susceptible for each isolate) used according to the CLSI clinical breakpoints for Actinobacillus pleuropneumoniae (APP), Pasteurella multocida (PM), and Escherichia coli (EC) for quinolones and cephalosporins, and the EUCAST guidelines for colistin in the case of E. coli , respectively.

    Techniques Used:

    actinobacillus pleuropneumoniae  (ATCC)


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    ATCC actinobacillus pleuropneumoniae
    Actinobacillus Pleuropneumoniae, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    actinobacillus pleuropneumoniae  (ATCC)


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    ATCC actinobacillus pleuropneumoniae
    Actinobacillus Pleuropneumoniae, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    actinobacillus pleuropneumoniae  (ATCC)


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    ATCC actinobacillus pleuropneumoniae
    Minimum inhibitory concentration (MIC) distribution values of <t> Actinobacillus pleuropneumoniae </t> to quinolones (enrofloxacin and marbofloxacin), tetracyclines (doxycycline and oxytetracycline), beta-lactams (ceftiofur and amoxicillin), phenicols (florfenicol), sulfonamides (Sulfamethoxazol/trimethoprim), pleuromutilins (tiamulin) and macrolides (tilmicosin, tildipirosin and tulathromycin) from 2019 to 2022 in Spain
    Actinobacillus Pleuropneumoniae, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/actinobacillus pleuropneumoniae/product/ATCC
    Average 86 stars, based on 1 article reviews
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    1) Product Images from "Susceptibility trends of swine respiratory pathogens from 2019 to 2022 to antimicrobials commonly used in Spain"

    Article Title: Susceptibility trends of swine respiratory pathogens from 2019 to 2022 to antimicrobials commonly used in Spain

    Journal: Porcine Health Management

    doi: 10.1186/s40813-023-00341-x

    Minimum inhibitory concentration (MIC) distribution values of  Actinobacillus pleuropneumoniae  to quinolones (enrofloxacin and marbofloxacin), tetracyclines (doxycycline and oxytetracycline), beta-lactams (ceftiofur and amoxicillin), phenicols (florfenicol), sulfonamides (Sulfamethoxazol/trimethoprim), pleuromutilins (tiamulin) and macrolides (tilmicosin, tildipirosin and tulathromycin) from 2019 to 2022 in Spain
    Figure Legend Snippet: Minimum inhibitory concentration (MIC) distribution values of Actinobacillus pleuropneumoniae to quinolones (enrofloxacin and marbofloxacin), tetracyclines (doxycycline and oxytetracycline), beta-lactams (ceftiofur and amoxicillin), phenicols (florfenicol), sulfonamides (Sulfamethoxazol/trimethoprim), pleuromutilins (tiamulin) and macrolides (tilmicosin, tildipirosin and tulathromycin) from 2019 to 2022 in Spain

    Techniques Used: Concentration Assay

    The adjusted odds ratio (95% confidence interval) describing the annual variation in susceptibility of  Actinobacillus pleuropneumoniae  (APP) isolates to quinolones (enrofloxacin and marbofloxacin), tetracyclines (doxycycline and oxytetracycline), beta-lactams (ceftiofur and amoxicillin), phenicols (florfenicol), sulfonamides (sulfamethoxazol/trimethoprim), pleuromutilins (tiamulin) and macrolides (tilmicosin, tulathromycin and tildipirosin) using the logistic regression model
    Figure Legend Snippet: The adjusted odds ratio (95% confidence interval) describing the annual variation in susceptibility of Actinobacillus pleuropneumoniae (APP) isolates to quinolones (enrofloxacin and marbofloxacin), tetracyclines (doxycycline and oxytetracycline), beta-lactams (ceftiofur and amoxicillin), phenicols (florfenicol), sulfonamides (sulfamethoxazol/trimethoprim), pleuromutilins (tiamulin) and macrolides (tilmicosin, tulathromycin and tildipirosin) using the logistic regression model

    Techniques Used:

    Percentage of susceptible isolates of Actinobacillus pleuropneumoniae by year for quinolones (enrofloxacin (circle) and marbofloxacin (square) ( A )), tetracyclines (doxycycline (circle) and oxytetracycline (square) ( B )) and amoxicillin (circle), tiamulin (square) and tilmicosin (diamond) ( C )
    Figure Legend Snippet: Percentage of susceptible isolates of Actinobacillus pleuropneumoniae by year for quinolones (enrofloxacin (circle) and marbofloxacin (square) ( A )), tetracyclines (doxycycline (circle) and oxytetracycline (square) ( B )) and amoxicillin (circle), tiamulin (square) and tilmicosin (diamond) ( C )

    Techniques Used:

    actinobacillus pleuropneumoniae serotype 4  (ATCC)


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    ATCC actinobacillus pleuropneumoniae serotype 4
    Inclusivity testing of different serotypes and corresponding virulence-associated factor genes of S. suis isolates by thrA -LAMP.
    Actinobacillus Pleuropneumoniae Serotype 4, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Rapid Diagnostic of Streptococcus suis in Necropsy Samples of Pigs by thrA -Based Loop-Mediated Isothermal Amplification Assay"

    Article Title: Rapid Diagnostic of Streptococcus suis in Necropsy Samples of Pigs by thrA -Based Loop-Mediated Isothermal Amplification Assay

    Journal: Microorganisms

    doi: 10.3390/microorganisms11102447

    Inclusivity testing of different serotypes and corresponding virulence-associated factor genes of S. suis isolates by thrA -LAMP.
    Figure Legend Snippet: Inclusivity testing of different serotypes and corresponding virulence-associated factor genes of S. suis isolates by thrA -LAMP.

    Techniques Used:

    Exclusivity testing of non-target species by thrA -LAMP.
    Figure Legend Snippet: Exclusivity testing of non-target species by thrA -LAMP.

    Techniques Used:

    actinobacillus pleuropneumoniae serotype 6  (ATCC)


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    ATCC actinobacillus pleuropneumoniae serotype 6
    Exclusivity testing of non-target species by thrA -LAMP.
    Actinobacillus Pleuropneumoniae Serotype 6, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Rapid Diagnostic of Streptococcus suis in Necropsy Samples of Pigs by thrA -Based Loop-Mediated Isothermal Amplification Assay"

    Article Title: Rapid Diagnostic of Streptococcus suis in Necropsy Samples of Pigs by thrA -Based Loop-Mediated Isothermal Amplification Assay

    Journal: Microorganisms

    doi: 10.3390/microorganisms11102447

    Exclusivity testing of non-target species by thrA -LAMP.
    Figure Legend Snippet: Exclusivity testing of non-target species by thrA -LAMP.

    Techniques Used:

    fastidious qc strain actinobacillus pleuropneumoniae atcc 27090  (ATCC)


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    ATCC fastidious qc strain actinobacillus pleuropneumoniae atcc 27090
    Fastidious Qc Strain Actinobacillus Pleuropneumoniae Atcc 27090, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    actinobacillus pleuropneumoniae  (ATCC)


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    ATCC actinobacillus pleuropneumoniae
    Actinobacillus Pleuropneumoniae, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    actinobacillus pleuropneumoniae l20  (ATCC)


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    ATCC actinobacillus pleuropneumoniae l20
    Actinobacillus Pleuropneumoniae L20, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a pleuropneumoniae serotype 1  (ATCC)


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    ATCC a pleuropneumoniae serotype 1
    2-D DIGE of
    A Pleuropneumoniae Serotype 1, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Proteomic and immunoproteomic characterization of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae"

    Article Title: Proteomic and immunoproteomic characterization of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-23

    2-D DIGE of
    Figure Legend Snippet: 2-D DIGE of "detergent-wash" proteins from A. pleuropneumoniae serotypes 1, 2 and 5 (subunit vaccine) and serotype 7 . For analysis of the subunit vaccine serotype 1, 2, and 5 were labelled with Cy2 (shown in blue), Cy3 (shown in green) and Cy5 (shown in red), respectively, and subsequently pooled. For visualization of differences between the serotypes used for vaccine generation, we compared couples of serotypes 1 and 2 (A), serotypes 1 and 5 (B), and serotypes 2 and 5 (C). For comparison of the subunit vaccine to serotype 7, the subunit vaccine was labelled with Cy3 (shown in green) and the "detergent wash" of serotype 7 was labelled with Cy5 (shown in red, D). Spots with intensities considerably above that in one or the other serotype were analyzed by mass spectrometry from preparative gels of the respective serotype (Additional file , Figure S2). The annotation of the identified protein is given in the same colour as the labelling of the respective serotype. The numbers on each spot, that has been identified, are consecutive and allow the finding of the respective spot on preparative gels (Additional file , Figure S2).

    Techniques Used: Mass Spectrometry

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    ATCC a pleuropneumoniae serotype 1
    2-D DIGE of
    A Pleuropneumoniae Serotype 1, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC actinobacillus pleuropneumoniae
    Number of <t> Actinobacillus pleuropneumoniae </t> (APP), Pasteurella multocida , and Escherichia coli isolates isolated during the study period (2019–2022). The number of samples submitted per year is also provided in brackets to identify the suspected pathogen.
    Actinobacillus Pleuropneumoniae, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC actinobacillus pleuropneumoniae serotype 4
    Inclusivity testing of different serotypes and corresponding virulence-associated factor genes of S. suis isolates by thrA -LAMP.
    Actinobacillus Pleuropneumoniae Serotype 4, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC actinobacillus pleuropneumoniae serotype 6
    Exclusivity testing of non-target species by thrA -LAMP.
    Actinobacillus Pleuropneumoniae Serotype 6, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/actinobacillus pleuropneumoniae serotype 6/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    actinobacillus pleuropneumoniae serotype 6 - by Bioz Stars, 2024-04
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    ATCC fastidious qc strain actinobacillus pleuropneumoniae atcc 27090
    Exclusivity testing of non-target species by thrA -LAMP.
    Fastidious Qc Strain Actinobacillus Pleuropneumoniae Atcc 27090, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastidious qc strain actinobacillus pleuropneumoniae atcc 27090/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fastidious qc strain actinobacillus pleuropneumoniae atcc 27090 - by Bioz Stars, 2024-04
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    ATCC actinobacillus pleuropneumoniae l20
    Exclusivity testing of non-target species by thrA -LAMP.
    Actinobacillus Pleuropneumoniae L20, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/actinobacillus pleuropneumoniae l20/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    actinobacillus pleuropneumoniae l20 - by Bioz Stars, 2024-04
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    Image Search Results


    2-D DIGE of

    Journal: Proteome Science

    Article Title: Proteomic and immunoproteomic characterization of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae

    doi: 10.1186/1477-5956-9-23

    Figure Lengend Snippet: 2-D DIGE of "detergent-wash" proteins from A. pleuropneumoniae serotypes 1, 2 and 5 (subunit vaccine) and serotype 7 . For analysis of the subunit vaccine serotype 1, 2, and 5 were labelled with Cy2 (shown in blue), Cy3 (shown in green) and Cy5 (shown in red), respectively, and subsequently pooled. For visualization of differences between the serotypes used for vaccine generation, we compared couples of serotypes 1 and 2 (A), serotypes 1 and 5 (B), and serotypes 2 and 5 (C). For comparison of the subunit vaccine to serotype 7, the subunit vaccine was labelled with Cy3 (shown in green) and the "detergent wash" of serotype 7 was labelled with Cy5 (shown in red, D). Spots with intensities considerably above that in one or the other serotype were analyzed by mass spectrometry from preparative gels of the respective serotype (Additional file , Figure S2). The annotation of the identified protein is given in the same colour as the labelling of the respective serotype. The numbers on each spot, that has been identified, are consecutive and allow the finding of the respective spot on preparative gels (Additional file , Figure S2).

    Article Snippet: For preparation of surface-associated proteins, apxIIA deletion mutants of A. pleuropneumoniae serotype 1 (ATCC 27088), 2 (DM322/5), 5 (K1711/84) [ ] and the wild type of serotype 7 (AP76, [ ]) were incubated in 150 ml of supplemented PPLO medium to an OD 600 of 0.2.

    Techniques: Mass Spectrometry

    Number of  Actinobacillus pleuropneumoniae  (APP), Pasteurella multocida , and Escherichia coli isolates isolated during the study period (2019–2022). The number of samples submitted per year is also provided in brackets to identify the suspected pathogen.

    Journal: Antibiotics

    Article Title: The Susceptibility Trends of Respiratory and Enteric Porcine Pathogens to Last-Resource Antimicrobials

    doi: 10.3390/antibiotics12111575

    Figure Lengend Snippet: Number of Actinobacillus pleuropneumoniae (APP), Pasteurella multocida , and Escherichia coli isolates isolated during the study period (2019–2022). The number of samples submitted per year is also provided in brackets to identify the suspected pathogen.

    Article Snippet: Thus, Actinobacillus pleuropneumoniae (ATCC 27090™), Escherichia coli (ATCC 25922™), Streptococcus pneumoniae (ATCC 49619™), and Enterococcus faecalis (ATCC 29212™) were included as quality control following the CLSI recommendations [ , ].

    Techniques: Isolation

    Minimum inhibitory concentration (MIC) distribution values of (A)  Actinobacillus pleuropneumoniae  , (B) Pasteurella multocida , and (C) Escherichia coli to quinolones (enrofloxacin and marbofloxacin) from 2019 to 2022 in Spain. MIC range is the minimum and maximum MIC value observed. MIC90 and MIC50 values are the lowest concentration of the antibiotic at which 90 and 50% of the isolates were inhibited, respectively.

    Journal: Antibiotics

    Article Title: The Susceptibility Trends of Respiratory and Enteric Porcine Pathogens to Last-Resource Antimicrobials

    doi: 10.3390/antibiotics12111575

    Figure Lengend Snippet: Minimum inhibitory concentration (MIC) distribution values of (A) Actinobacillus pleuropneumoniae , (B) Pasteurella multocida , and (C) Escherichia coli to quinolones (enrofloxacin and marbofloxacin) from 2019 to 2022 in Spain. MIC range is the minimum and maximum MIC value observed. MIC90 and MIC50 values are the lowest concentration of the antibiotic at which 90 and 50% of the isolates were inhibited, respectively.

    Article Snippet: Thus, Actinobacillus pleuropneumoniae (ATCC 27090™), Escherichia coli (ATCC 25922™), Streptococcus pneumoniae (ATCC 49619™), and Enterococcus faecalis (ATCC 29212™) were included as quality control following the CLSI recommendations [ , ].

    Techniques: Concentration Assay

    Minimum inhibitory concentration (MIC) distribution values of  Actinobacillus pleuropneumoniae  , Pasteurella multocida , and Escherichia coli to ceftiofur and cefquinome (only Esherichia coli ) from 2019 to 2022 in Spain. MIC range is the minimum and maximum MIC value observed. MIC90 and MIC50 values are the lowest concentration of the antibiotic at which 90 and 50% of the isolates were inhibited, respectively.

    Journal: Antibiotics

    Article Title: The Susceptibility Trends of Respiratory and Enteric Porcine Pathogens to Last-Resource Antimicrobials

    doi: 10.3390/antibiotics12111575

    Figure Lengend Snippet: Minimum inhibitory concentration (MIC) distribution values of Actinobacillus pleuropneumoniae , Pasteurella multocida , and Escherichia coli to ceftiofur and cefquinome (only Esherichia coli ) from 2019 to 2022 in Spain. MIC range is the minimum and maximum MIC value observed. MIC90 and MIC50 values are the lowest concentration of the antibiotic at which 90 and 50% of the isolates were inhibited, respectively.

    Article Snippet: Thus, Actinobacillus pleuropneumoniae (ATCC 27090™), Escherichia coli (ATCC 25922™), Streptococcus pneumoniae (ATCC 49619™), and Enterococcus faecalis (ATCC 29212™) were included as quality control following the CLSI recommendations [ , ].

    Techniques: Concentration Assay

    Percentage of Actinobacillus pleuropneumoniae ( A ), Pasteurella multocida ( B ), and Escherichia coli ( C ), and isolates belonging to antimicrobial susceptibility category 1 (circle), category 2 (square), category 3 (diamond), and category 4 (triangle) for enrofloxacin. The number of categories for multinominal analysis was based on distributing the range of MIC values in four categories (from one to four), equally distributed, that include two MIC values per category, with category one being the most susceptible (lowest MIC value) and category four the least susceptible (highest MIC value).

    Journal: Antibiotics

    Article Title: The Susceptibility Trends of Respiratory and Enteric Porcine Pathogens to Last-Resource Antimicrobials

    doi: 10.3390/antibiotics12111575

    Figure Lengend Snippet: Percentage of Actinobacillus pleuropneumoniae ( A ), Pasteurella multocida ( B ), and Escherichia coli ( C ), and isolates belonging to antimicrobial susceptibility category 1 (circle), category 2 (square), category 3 (diamond), and category 4 (triangle) for enrofloxacin. The number of categories for multinominal analysis was based on distributing the range of MIC values in four categories (from one to four), equally distributed, that include two MIC values per category, with category one being the most susceptible (lowest MIC value) and category four the least susceptible (highest MIC value).

    Article Snippet: Thus, Actinobacillus pleuropneumoniae (ATCC 27090™), Escherichia coli (ATCC 25922™), Streptococcus pneumoniae (ATCC 49619™), and Enterococcus faecalis (ATCC 29212™) were included as quality control following the CLSI recommendations [ , ].

    Techniques:

    Percentage of susceptible isolates by year for enrofloxacin ( A ) and marbofloxacin ( B ) of Actinobacillus pleuropneumoniae (circle), Pasteurella multocida (square), and Escherichia coli (diamond), using the CLSI and EUCAST clinical breakpoints, as detailed in the Materials and Methods section.

    Journal: Antibiotics

    Article Title: The Susceptibility Trends of Respiratory and Enteric Porcine Pathogens to Last-Resource Antimicrobials

    doi: 10.3390/antibiotics12111575

    Figure Lengend Snippet: Percentage of susceptible isolates by year for enrofloxacin ( A ) and marbofloxacin ( B ) of Actinobacillus pleuropneumoniae (circle), Pasteurella multocida (square), and Escherichia coli (diamond), using the CLSI and EUCAST clinical breakpoints, as detailed in the Materials and Methods section.

    Article Snippet: Thus, Actinobacillus pleuropneumoniae (ATCC 27090™), Escherichia coli (ATCC 25922™), Streptococcus pneumoniae (ATCC 49619™), and Enterococcus faecalis (ATCC 29212™) were included as quality control following the CLSI recommendations [ , ].

    Techniques:

    The adjusted odds ratio (95% confidence interval) describing the annual variation in the susceptibility of  A. pleuropneumoniae  isolates to enrofloxacin using the logistic and multinominal regression model. The number of  A. pleuropneumoniae  isolates by year is detailed in <xref ref-type= Table 1 ." width="100%" height="100%">

    Journal: Antibiotics

    Article Title: The Susceptibility Trends of Respiratory and Enteric Porcine Pathogens to Last-Resource Antimicrobials

    doi: 10.3390/antibiotics12111575

    Figure Lengend Snippet: The adjusted odds ratio (95% confidence interval) describing the annual variation in the susceptibility of A. pleuropneumoniae isolates to enrofloxacin using the logistic and multinominal regression model. The number of A. pleuropneumoniae isolates by year is detailed in Table 1 .

    Article Snippet: Thus, Actinobacillus pleuropneumoniae (ATCC 27090™), Escherichia coli (ATCC 25922™), Streptococcus pneumoniae (ATCC 49619™), and Enterococcus faecalis (ATCC 29212™) were included as quality control following the CLSI recommendations [ , ].

    Techniques:

    Percentage of Actinobacillus pleuropneumoniae ( A ), Pasteurella multocida ( B ), and Escherichia coli ( C ), and isolates belonging to antimicrobial susceptibility category 1 (circle), category 2 (square), category 3 (diamond), and category 4 (triangle) for marbofloxacin. The number of categories for multinominal analysis was based on distributing the range of MIC values in four categories (from one to four), equally distributed, that include two MIC values per category, with category one being the most susceptible (lowest MIC value) and category four the least susceptible (highest MIC value).

    Journal: Antibiotics

    Article Title: The Susceptibility Trends of Respiratory and Enteric Porcine Pathogens to Last-Resource Antimicrobials

    doi: 10.3390/antibiotics12111575

    Figure Lengend Snippet: Percentage of Actinobacillus pleuropneumoniae ( A ), Pasteurella multocida ( B ), and Escherichia coli ( C ), and isolates belonging to antimicrobial susceptibility category 1 (circle), category 2 (square), category 3 (diamond), and category 4 (triangle) for marbofloxacin. The number of categories for multinominal analysis was based on distributing the range of MIC values in four categories (from one to four), equally distributed, that include two MIC values per category, with category one being the most susceptible (lowest MIC value) and category four the least susceptible (highest MIC value).

    Article Snippet: Thus, Actinobacillus pleuropneumoniae (ATCC 27090™), Escherichia coli (ATCC 25922™), Streptococcus pneumoniae (ATCC 49619™), and Enterococcus faecalis (ATCC 29212™) were included as quality control following the CLSI recommendations [ , ].

    Techniques:

    The adjusted odds ratio (95% confidence interval) describing the annual variation in the susceptibility of  A. pleuropneumoniae  isolates to marbofloxacin using the logistic and multinominal regression model. The number of  A. pleuropneumoniae  isolates by year is detailed in <xref ref-type= Table 1 ." width="100%" height="100%">

    Journal: Antibiotics

    Article Title: The Susceptibility Trends of Respiratory and Enteric Porcine Pathogens to Last-Resource Antimicrobials

    doi: 10.3390/antibiotics12111575

    Figure Lengend Snippet: The adjusted odds ratio (95% confidence interval) describing the annual variation in the susceptibility of A. pleuropneumoniae isolates to marbofloxacin using the logistic and multinominal regression model. The number of A. pleuropneumoniae isolates by year is detailed in Table 1 .

    Article Snippet: Thus, Actinobacillus pleuropneumoniae (ATCC 27090™), Escherichia coli (ATCC 25922™), Streptococcus pneumoniae (ATCC 49619™), and Enterococcus faecalis (ATCC 29212™) were included as quality control following the CLSI recommendations [ , ].

    Techniques:

    Percentage of Actinobacillus pleuropneumoniae ( A ), Pasteurella multocida ( B ), and Escherichia coli ( C ) isolates belonging to antimicrobial susceptibility category 1 (circle), category 2 (square), category 3 (diamond), and category 4 (triangle) for ceftiofur. The number of categories for multinominal analysis was based on distributing the range of MIC values in four categories (from one to four), equally distributed, that include two MIC values per category, with category one being the most susceptible (lowest MIC value) and category four the least susceptible (highest MIC value).

    Journal: Antibiotics

    Article Title: The Susceptibility Trends of Respiratory and Enteric Porcine Pathogens to Last-Resource Antimicrobials

    doi: 10.3390/antibiotics12111575

    Figure Lengend Snippet: Percentage of Actinobacillus pleuropneumoniae ( A ), Pasteurella multocida ( B ), and Escherichia coli ( C ) isolates belonging to antimicrobial susceptibility category 1 (circle), category 2 (square), category 3 (diamond), and category 4 (triangle) for ceftiofur. The number of categories for multinominal analysis was based on distributing the range of MIC values in four categories (from one to four), equally distributed, that include two MIC values per category, with category one being the most susceptible (lowest MIC value) and category four the least susceptible (highest MIC value).

    Article Snippet: Thus, Actinobacillus pleuropneumoniae (ATCC 27090™), Escherichia coli (ATCC 25922™), Streptococcus pneumoniae (ATCC 49619™), and Enterococcus faecalis (ATCC 29212™) were included as quality control following the CLSI recommendations [ , ].

    Techniques:

    Percentage of susceptible isolates by year for ceftiofur ( A ) of Actinobacillus pleuropneumoniae (circle), Pasteurella multocida (square), and Escherichia coli (diamond), as well as cefquinome ( B ) of Escherichia coli (diamond), using the CLSI and EUCAST clinical breakpoints, as detailed in the Materials and Methods section.

    Journal: Antibiotics

    Article Title: The Susceptibility Trends of Respiratory and Enteric Porcine Pathogens to Last-Resource Antimicrobials

    doi: 10.3390/antibiotics12111575

    Figure Lengend Snippet: Percentage of susceptible isolates by year for ceftiofur ( A ) of Actinobacillus pleuropneumoniae (circle), Pasteurella multocida (square), and Escherichia coli (diamond), as well as cefquinome ( B ) of Escherichia coli (diamond), using the CLSI and EUCAST clinical breakpoints, as detailed in the Materials and Methods section.

    Article Snippet: Thus, Actinobacillus pleuropneumoniae (ATCC 27090™), Escherichia coli (ATCC 25922™), Streptococcus pneumoniae (ATCC 49619™), and Enterococcus faecalis (ATCC 29212™) were included as quality control following the CLSI recommendations [ , ].

    Techniques:

    Clinical breakpoints (susceptible/non-susceptible for each isolate) used according to the CLSI clinical breakpoints for  Actinobacillus pleuropneumoniae  (APP), Pasteurella multocida (PM), and Escherichia coli (EC) for quinolones and cephalosporins, and the EUCAST guidelines for colistin in the case of E. coli , respectively.

    Journal: Antibiotics

    Article Title: The Susceptibility Trends of Respiratory and Enteric Porcine Pathogens to Last-Resource Antimicrobials

    doi: 10.3390/antibiotics12111575

    Figure Lengend Snippet: Clinical breakpoints (susceptible/non-susceptible for each isolate) used according to the CLSI clinical breakpoints for Actinobacillus pleuropneumoniae (APP), Pasteurella multocida (PM), and Escherichia coli (EC) for quinolones and cephalosporins, and the EUCAST guidelines for colistin in the case of E. coli , respectively.

    Article Snippet: Thus, Actinobacillus pleuropneumoniae (ATCC 27090™), Escherichia coli (ATCC 25922™), Streptococcus pneumoniae (ATCC 49619™), and Enterococcus faecalis (ATCC 29212™) were included as quality control following the CLSI recommendations [ , ].

    Techniques:

    Inclusivity testing of different serotypes and corresponding virulence-associated factor genes of S. suis isolates by thrA -LAMP.

    Journal: Microorganisms

    Article Title: Rapid Diagnostic of Streptococcus suis in Necropsy Samples of Pigs by thrA -Based Loop-Mediated Isothermal Amplification Assay

    doi: 10.3390/microorganisms11102447

    Figure Lengend Snippet: Inclusivity testing of different serotypes and corresponding virulence-associated factor genes of S. suis isolates by thrA -LAMP.

    Article Snippet: Actinobacillus pleuropneumoniae Serotype 4 (ATCC 33378) , − , − , 1.

    Techniques:

    Exclusivity testing of non-target species by thrA -LAMP.

    Journal: Microorganisms

    Article Title: Rapid Diagnostic of Streptococcus suis in Necropsy Samples of Pigs by thrA -Based Loop-Mediated Isothermal Amplification Assay

    doi: 10.3390/microorganisms11102447

    Figure Lengend Snippet: Exclusivity testing of non-target species by thrA -LAMP.

    Article Snippet: Actinobacillus pleuropneumoniae Serotype 4 (ATCC 33378) , − , − , 1.

    Techniques:

    Exclusivity testing of non-target species by thrA -LAMP.

    Journal: Microorganisms

    Article Title: Rapid Diagnostic of Streptococcus suis in Necropsy Samples of Pigs by thrA -Based Loop-Mediated Isothermal Amplification Assay

    doi: 10.3390/microorganisms11102447

    Figure Lengend Snippet: Exclusivity testing of non-target species by thrA -LAMP.

    Article Snippet: Actinobacillus pleuropneumoniae Serotype 6 (ATCC 33590) , − , − , 1.

    Techniques: