acn  (Thermo Fisher)


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  • 99
    Name:
    Acetonitrile in Water 20
    Description:
    This reagent is intended for N terminal protein sequencing applications Use with other protein sequencing reagents 20 Acetonitrile in Water S4B 200 mL Procise Sequencing System Model 492 and 294 5 PITC in N Heptane 40 mL 25 Trifluoroacetic Acid in Water 40 mL Ethyl Acetate 450 mL N Butyl Chloride 200 mL Solvent A3 3 5 THF⁄Water 960 mL Solvent B2 Isopropanol and Acetonitrile 1 L For Research Use Only Not for use in diagnostics procedures
    Catalog Number:
    400314
    Price:
    None
    Applications:
    Protein Biology|Protein Sequencing|Protein Sequencing & Peptide Synthesis
    Category:
    Lab Reagents and Chemicals
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    Structured Review

    Thermo Fisher acn
    This reagent is intended for N terminal protein sequencing applications Use with other protein sequencing reagents 20 Acetonitrile in Water S4B 200 mL Procise Sequencing System Model 492 and 294 5 PITC in N Heptane 40 mL 25 Trifluoroacetic Acid in Water 40 mL Ethyl Acetate 450 mL N Butyl Chloride 200 mL Solvent A3 3 5 THF⁄Water 960 mL Solvent B2 Isopropanol and Acetonitrile 1 L For Research Use Only Not for use in diagnostics procedures
    https://www.bioz.com/result/acn/product/Thermo Fisher
    Average 99 stars, based on 81 article reviews
    Price from $9.99 to $1999.99
    acn - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: In Vitro Metabolites of Di-2-ethylhexyl Adipate (DEHA) as Biomarkers of Exposure in Human Biomonitoring Applications
    Article Snippet: .. Details on the HPLC gradient for the separation of DEHA and DEHP metabolites and the method for online SPE are presented elsewhere., Briefly, metabolites in the supernatant of the human liver microsomal homogenate (500 μ L), obtained after incubating for 5 h with DEHA and MEHA, were extracted using online SPE on a Chromolith RP-18 precolumn (Merck KGaA, Darmstadt, Germany), resolved on a Betasil phenyl HPLC column (3 μ M, 2.1 mm × 25 mm, ThermoFisher Scientific, San Jose, CA, USA) using a water/acetonitrile gradient program and detected by mass spectrometry on a TSQ Vantage AM triple quadrupole mass spectrometer (ThermoFisher Scientific, San Jose, CA, USA). ..

    Article Title: Measurement of DNA concentration as a normalization strategy for metabolomic data from adherent cell lines
    Article Snippet: .. HPLC-grade water (H2 O) and acetonitrile (ACN) were purchased from Thermo Fisher Scientific (Pittsburgh, PA, USA). .. To determine the accuracy of total protein measurement, serial dilutions of 0.25, 0.5, 1, 2, and 4 million OVCAR-8 cells were seeded in 10 cm dishes (Corning, Tewksbury, MA) in triplicate.

    Centrifugation:

    Article Title: The Differential Absorption of a Series of P-Glycoprotein Substrates in Isolated Perfused Lungs from Mdr1a/1b Genetic Knockout Mice can be Attributed to Distinct Physico-Chemical Properties: an Insight into Predicting Transporter-Mediated, Pulmonary Specific Disposition
    Article Snippet: .. Analysis for non-radiolabelled substrates involved deproteinisation by precipitation with 2x volume of ice-cold acetonitrile (ACN) or methanol (MeOH) to the sample followed by centrifugation (20,000 g 15 min at 4°C) with the resulting protein-free supernatant undergoing analysis by LC-MS or LC-MS/MS ESI + ve ionisation (Thermo Finnigan LCQ Classic). .. Samples were analysed using a C-18 HPLC column (Kromasil, 100 Å pore, 3.5 μm particle size) at 30°C with a mobile phase (0.2 mL/min) comprising a various gradients of solvent A: MeOH or ACN, and solvent B: H20 + 0.1% Formic Acid; MS/MS was used for domperidone, mitoxantrone, monensin, saquinavir.

    Mass Spectrometry:

    Article Title: In Vitro Metabolites of Di-2-ethylhexyl Adipate (DEHA) as Biomarkers of Exposure in Human Biomonitoring Applications
    Article Snippet: .. Details on the HPLC gradient for the separation of DEHA and DEHP metabolites and the method for online SPE are presented elsewhere., Briefly, metabolites in the supernatant of the human liver microsomal homogenate (500 μ L), obtained after incubating for 5 h with DEHA and MEHA, were extracted using online SPE on a Chromolith RP-18 precolumn (Merck KGaA, Darmstadt, Germany), resolved on a Betasil phenyl HPLC column (3 μ M, 2.1 mm × 25 mm, ThermoFisher Scientific, San Jose, CA, USA) using a water/acetonitrile gradient program and detected by mass spectrometry on a TSQ Vantage AM triple quadrupole mass spectrometer (ThermoFisher Scientific, San Jose, CA, USA). ..

    Article Title: Calcium-binding protein S100A4 confers mesenchymal progenitor cell fibrogenicity in idiopathic pulmonary fibrosis
    Article Snippet: .. Peptide samples were resuspended in 98:2 water/acetonitrile, 0.1% formic acid and run on an Orbitrap Velos (Thermo Fisher Scientific) mass spectrometer. .. The Thermo RAW files were analyzed with Peaks Studio 7.0 build 20140912 (Bioinformatics Solutions) software package for interpretation of tandem mass spectometry and protein inference.

    Transferring:

    Article Title: Expression, purification, and characterization of recombinant human transferrin from rice (Oryza sativa L.)
    Article Snippet: .. These iron-free or apo-TFs were further desalted using ZipTip™ μ-C18 pipette tips (Millipore, Billerica, MA), eluted with a solution of 70% acetonitrile (ACN), 0.2% formic acid, and 5 mg/ml MALDI matrix (α-cyano-4-hydroxycinnamic acid), and spotted onto the MALDI target and analyzed with an Applied Biosystems 4700 Proteomics Analyzer (Applied Biosystems Inc., Foster City, CA) at the Molecular Structure Facility at the University of California, Davis, CA, US. .. To evaluate the glycosylation status of rice-derived rhTF, the purified rhTF protein was subjected to digestion with peptide-N-glycosidase F (PNGase F) (Sigma, St. Louis, MO).

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: The Differential Absorption of a Series of P-Glycoprotein Substrates in Isolated Perfused Lungs from Mdr1a/1b Genetic Knockout Mice can be Attributed to Distinct Physico-Chemical Properties: an Insight into Predicting Transporter-Mediated, Pulmonary Specific Disposition
    Article Snippet: .. Analysis for non-radiolabelled substrates involved deproteinisation by precipitation with 2x volume of ice-cold acetonitrile (ACN) or methanol (MeOH) to the sample followed by centrifugation (20,000 g 15 min at 4°C) with the resulting protein-free supernatant undergoing analysis by LC-MS or LC-MS/MS ESI + ve ionisation (Thermo Finnigan LCQ Classic). .. Samples were analysed using a C-18 HPLC column (Kromasil, 100 Å pore, 3.5 μm particle size) at 30°C with a mobile phase (0.2 mL/min) comprising a various gradients of solvent A: MeOH or ACN, and solvent B: H20 + 0.1% Formic Acid; MS/MS was used for domperidone, mitoxantrone, monensin, saquinavir.

    Injection:

    Article Title: “Yellow” laccase from Sclerotinia sclerotiorum is a blue laccase that enhances its substrate affinity by forming a reversible tyrosyl-product adduct
    Article Snippet: .. Liquid chromatography separations were achieved on a C18 VYDAC (4.6 mmx150 mm, 5 μm) column, eluting with a linear gradient of 10%-50% acetonitrile/water (containing 0.1%TFA) for 40 min; total run time 55 min; flow rate 1 mL/min; injection volume 20 μL or a Hypersil Gold, ThermoScientific (2.1 mm x 45 mm, 1.9 μm) column, eluting with the following gradient: 3%-10% acetonitrile/water (0.1%TFA) for 4 min., 10%-20% acetonitrile/water (0.1%TFA) for 10 min and 20%-45% acetonitrile/water (0.1%TFA) for 5 min.; total run time 25 min; flow rate 0.25 ml/min; injection volume 2 μL. ..

    Liquid Chromatography:

    Article Title: “Yellow” laccase from Sclerotinia sclerotiorum is a blue laccase that enhances its substrate affinity by forming a reversible tyrosyl-product adduct
    Article Snippet: .. Liquid chromatography separations were achieved on a C18 VYDAC (4.6 mmx150 mm, 5 μm) column, eluting with a linear gradient of 10%-50% acetonitrile/water (containing 0.1%TFA) for 40 min; total run time 55 min; flow rate 1 mL/min; injection volume 20 μL or a Hypersil Gold, ThermoScientific (2.1 mm x 45 mm, 1.9 μm) column, eluting with the following gradient: 3%-10% acetonitrile/water (0.1%TFA) for 4 min., 10%-20% acetonitrile/water (0.1%TFA) for 10 min and 20%-45% acetonitrile/water (0.1%TFA) for 5 min.; total run time 25 min; flow rate 0.25 ml/min; injection volume 2 μL. ..

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  • 90
    Thermo Fisher s cerevisiae teto acs2 strain
    Chemical-induced haploinsufficiency identifies acetyl CoA synthetase as a candidate target for AR-12. (A) S. <t>cerevisiae</t> heterozygous deletion mutants of the listed genes show altered AR-12 susceptibility in a chemical-induced haploinsufficiency screen. (B) Essential genes with fitness defects in the screen. Bars indicate mean fitness defects with error bars indicating the standard deviation of four independent experiments. (C) Relative growth of C. albicans acs 2Δ/ <t>ACS2</t> normalized to reference strain in the presence and absence of AR-12. Data are from two to four replicates with a standard deviation indicated by error bars.
    S Cerevisiae Teto Acs2 Strain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s cerevisiae teto acs2 strain/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    s cerevisiae teto acs2 strain - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    99
    Thermo Fisher acetonitrile acn
    Chromatograms of rutin (A) and anthocyanins (B) of TF by UPLC-TUV. Rutin and anthocyanins were separated on the same Acquity UPLC BEH C18 column (2.1x100 mm, 1.7 μm, Waters, Milford, MA). Rutin separation used <t>MeOH</t> and 0.1% H 3 PO 4 as the mobile phase at 0.21 mL/min with detection at 358 nm. Anthocyanins separation used <t>ACN</t> and 0.2% H 3 PO 4 as the mobile phase at 0.3 mL/min and detection at 520 nm. Rutin: quercetin-3-O-rutinlside; C3G: cyanidin-3-O-glucoside; C3R: cyanidin-3-O-rutinoside; and TF: total flavonoids.
    Acetonitrile Acn, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acetonitrile acn/product/Thermo Fisher
    Average 99 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    acetonitrile acn - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher hplc grade acetonitrile acn
    Chromatograms of rutin (A) and anthocyanins (B) of TF by UPLC-TUV. Rutin and anthocyanins were separated on the same Acquity UPLC BEH C18 column (2.1x100 mm, 1.7 μm, Waters, Milford, MA). Rutin separation used <t>MeOH</t> and 0.1% H 3 PO 4 as the mobile phase at 0.21 mL/min with detection at 358 nm. Anthocyanins separation used <t>ACN</t> and 0.2% H 3 PO 4 as the mobile phase at 0.3 mL/min and detection at 520 nm. Rutin: quercetin-3-O-rutinlside; C3G: cyanidin-3-O-glucoside; C3R: cyanidin-3-O-rutinoside; and TF: total flavonoids.
    Hplc Grade Acetonitrile Acn, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc grade acetonitrile acn/product/Thermo Fisher
    Average 92 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    hplc grade acetonitrile acn - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Chemical-induced haploinsufficiency identifies acetyl CoA synthetase as a candidate target for AR-12. (A) S. cerevisiae heterozygous deletion mutants of the listed genes show altered AR-12 susceptibility in a chemical-induced haploinsufficiency screen. (B) Essential genes with fitness defects in the screen. Bars indicate mean fitness defects with error bars indicating the standard deviation of four independent experiments. (C) Relative growth of C. albicans acs 2Δ/ ACS2 normalized to reference strain in the presence and absence of AR-12. Data are from two to four replicates with a standard deviation indicated by error bars.

    Journal: ACS Infectious Diseases

    Article Title: Antitumor/Antifungal Celecoxib Derivative AR-12 is a Non-Nucleoside Inhibitor of the ANL-Family Adenylating Enzyme Acetyl CoA Synthetase

    doi: 10.1021/acsinfecdis.5b00134

    Figure Lengend Snippet: Chemical-induced haploinsufficiency identifies acetyl CoA synthetase as a candidate target for AR-12. (A) S. cerevisiae heterozygous deletion mutants of the listed genes show altered AR-12 susceptibility in a chemical-induced haploinsufficiency screen. (B) Essential genes with fitness defects in the screen. Bars indicate mean fitness defects with error bars indicating the standard deviation of four independent experiments. (C) Relative growth of C. albicans acs 2Δ/ ACS2 normalized to reference strain in the presence and absence of AR-12. Data are from two to four replicates with a standard deviation indicated by error bars.

    Article Snippet: The S. cerevisiae tetO-ACS2 strain was obtained from ThermoScientific.

    Techniques: Standard Deviation

    AR-12 and genetic depletion of Acs2 lead to a loss of cellular integrity. (A) Cells were treated with DMSO or AR-12 (8 μg/mL; MIC concentration under these conditions) for 6 h in YPD medium at 30 °C. Cells were harvested, stained with propidium iodide, and photographed under bright field and fluorescent channels. (B) Yeast strains with and without a doxycycline ( Teto 7 )-repressed ACS2 allele were grown overnight in medium containing doxycycline or DMSO. The cells were processed as in panel A. See the text for quantification.

    Journal: ACS Infectious Diseases

    Article Title: Antitumor/Antifungal Celecoxib Derivative AR-12 is a Non-Nucleoside Inhibitor of the ANL-Family Adenylating Enzyme Acetyl CoA Synthetase

    doi: 10.1021/acsinfecdis.5b00134

    Figure Lengend Snippet: AR-12 and genetic depletion of Acs2 lead to a loss of cellular integrity. (A) Cells were treated with DMSO or AR-12 (8 μg/mL; MIC concentration under these conditions) for 6 h in YPD medium at 30 °C. Cells were harvested, stained with propidium iodide, and photographed under bright field and fluorescent channels. (B) Yeast strains with and without a doxycycline ( Teto 7 )-repressed ACS2 allele were grown overnight in medium containing doxycycline or DMSO. The cells were processed as in panel A. See the text for quantification.

    Article Snippet: The S. cerevisiae tetO-ACS2 strain was obtained from ThermoScientific.

    Techniques: Concentration Assay, Staining

    Chromatograms of rutin (A) and anthocyanins (B) of TF by UPLC-TUV. Rutin and anthocyanins were separated on the same Acquity UPLC BEH C18 column (2.1x100 mm, 1.7 μm, Waters, Milford, MA). Rutin separation used MeOH and 0.1% H 3 PO 4 as the mobile phase at 0.21 mL/min with detection at 358 nm. Anthocyanins separation used ACN and 0.2% H 3 PO 4 as the mobile phase at 0.3 mL/min and detection at 520 nm. Rutin: quercetin-3-O-rutinlside; C3G: cyanidin-3-O-glucoside; C3R: cyanidin-3-O-rutinoside; and TF: total flavonoids.

    Journal: PLoS ONE

    Article Title: Anti-Inflammatory and Antinociceptive Properties of Flavonoids from the Fruits of Black Mulberry (Morus nigra L.)

    doi: 10.1371/journal.pone.0153080

    Figure Lengend Snippet: Chromatograms of rutin (A) and anthocyanins (B) of TF by UPLC-TUV. Rutin and anthocyanins were separated on the same Acquity UPLC BEH C18 column (2.1x100 mm, 1.7 μm, Waters, Milford, MA). Rutin separation used MeOH and 0.1% H 3 PO 4 as the mobile phase at 0.21 mL/min with detection at 358 nm. Anthocyanins separation used ACN and 0.2% H 3 PO 4 as the mobile phase at 0.3 mL/min and detection at 520 nm. Rutin: quercetin-3-O-rutinlside; C3G: cyanidin-3-O-glucoside; C3R: cyanidin-3-O-rutinoside; and TF: total flavonoids.

    Article Snippet: Acetonitrile (ACN) and methanol (MeOH) for UPLC analysis were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: