|
Carlo Erba Reagents
acetonitrile acn hplc grade Acetonitrile Acn Hplc Grade, supplied by Carlo Erba Reagents, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/acetonitrile acn hplc grade/product/Carlo Erba Reagents Average 86 stars, based on 1 article reviews
acetonitrile acn hplc grade - by Bioz Stars,
2026-05
86/100 stars
|
Buy from Supplier |
|
Fisher Scientific
acn ![]() Acn, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/acn/product/Fisher Scientific Average 86 stars, based on 1 article reviews
acn - by Bioz Stars,
2026-05
86/100 stars
|
Buy from Supplier |
|
Fisher Scientific
v v acn milli q purified water milli q purified water ![]() V V Acn Milli Q Purified Water Milli Q Purified Water, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/v v acn milli q purified water milli q purified water/product/Fisher Scientific Average 86 stars, based on 1 article reviews
v v acn milli q purified water milli q purified water - by Bioz Stars,
2026-05
86/100 stars
|
Buy from Supplier |
|
Loba Chemie
acetic anhydride cas number 108 24 7 acetonitrile acn cas number 75 05 8 ![]() Acetic Anhydride Cas Number 108 24 7 Acetonitrile Acn Cas Number 75 05 8, supplied by Loba Chemie, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/acetic anhydride cas number 108 24 7 acetonitrile acn cas number 75 05 8/product/Loba Chemie Average 86 stars, based on 1 article reviews
acetic anhydride cas number 108 24 7 acetonitrile acn cas number 75 05 8 - by Bioz Stars,
2026-05
86/100 stars
|
Buy from Supplier |
|
Carlo Erba Reagents
hplc gradient grade acetonitrile acn ![]() Hplc Gradient Grade Acetonitrile Acn, supplied by Carlo Erba Reagents, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/hplc gradient grade acetonitrile acn/product/Carlo Erba Reagents Average 86 stars, based on 1 article reviews
hplc gradient grade acetonitrile acn - by Bioz Stars,
2026-05
86/100 stars
|
Buy from Supplier |
Journal: Current Protocols
Article Title: A Facile Strategy for 3′‐Terminal Functionalization of DNA and RNA via On‐Column Conjugation with Integration into Tandem Oligonucleotide Synthesis
doi: 10.1002/cpz1.70374
Figure Lengend Snippet: ( A ) Synthesis of the crude 5′‐ O ‐(4,4′‐dimethoxytrityl)‐3′‐ O ‐sucinimide thymidine ( 1 ) for use in solid support reactions. ( B ) Activation of R ‐CPG using DSC‐mediated mixed carbonate synthesis. ( C ) On‐solid‐support construction of a putrescine carbamate‐linked nucleoside, which can be used for strand elongation subsequently. Reagent abbreviations: DSC, N,N ′‐disuccinimidyl carbonate, CPR II CPG, chemical phosphorylation reagent II control pore glass. Note : All round‐bottom flasks should be oven‐dried at 110°C for at least 2 hr before DSC activation to ensure complete removal of residual moisture. In addition, 3‐Å or 4‐Å molecular sieves should be preactivated if long‐term storage, defined as 2‐3 days, of the crude carbonate solution is anticipated, as they help maintain anhydrous conditions and minimize hydrolysis of the mixed carbonate. An automated dispensing pipettor should be used for measuring liquid volumes, and a scoopula should be used for transferring solid reagents to ensure accuracy and reproducibility. The following reagents are needed to make a mixed DNA oligonucleotide: 3% (w/v) trichloroacetic acid in dichloromethane (DCM; ChemGenes, cat. no. RN‐1462); cap A (acetic anhydride/pyridine/tetrahydrofuran [THF]; ChemGenes, cat. no. RN‐1458); cap B (10% N ‐methylimidazole in THF; ChemGenes, cat. no. RN‐1481); activation reagent (0.25 M of 5‐ethylthio‐tetrazole in ACN; ChemGenes, cat. no. RN‐1466); oxidation solution (0.02 M iodine/pyridine/H 2 O/THF; ChemGenes, cat. no. RN‐1455); acetonitrile (ACN), DNA synthesis grade (Fisher Scientific, cat. no. A998‐4); 2′‐deoxyadenosine (n‐bz) CED phosphoramidite (ChemGenes, cat. no. ANP‐5551); 2′‐deoxyguanosine (n‐ibu) CED phosphoramidite (ChemGenes, cat. no. ANP‐5553); 2′‐deoxycytidine ( n ‐acetyl) CED phosphoramidite (ChemGenes, cat. no. ANP‐5560); and thymidine CED phosphoramidite (ChemGenes, cat. no. ANP‐5554).
Article Snippet: In a 1‐liter glass bottle, add 100 ml
Techniques: Activation Assay, Phospho-proteomics, Control, Transferring, DNA Synthesis