Journal: Neoplasia (New York, N.Y.)

Article Title: Metabolic regulation of histone acetylation by ACLY supports MDR1 expression in colorectal cancer and highlights a targetable vulnerability

doi: 10.1016/j.neo.2026.101314

Figure Lengend Snippet: ACLY activity regulates MDR1 expression in colorectal cancer. (A) Transcript levels of ACLY and MDR1 (ABCB1) in colorectal cancer (red) and normal colon tissues (grey) analyzed using GEPIA (TCGA/GTEx datasets). (B) Immunoblot analysis of MDR1 in SW480 and DLD1 cells treated with the ACLY inhibitor BMS-303141 (20 or 50 μM, 48 h). (C) Immunoblot analysis of ACLY and MDR1 in SW480 and DLD1 cells transduced with empty vector (pLV) or ACLY-overexpressing vector (pLV[Exp]-hACLY). (D) Immunoblot analysis of ACLY and MDR1 in control and ACLY-overexpressing cells treated with BMS-303141 (50 μM, 48 h). (E) Relative ABCB1 mRNA levels in control and ACLY-overexpressing cells, and in cells treated with BMS-303141. (F) Relative ACLY mRNA levels under the same conditions. Data are presented as mean ± SD (n = 3 unless otherwise indicated). Statistical significance was determined using unpaired two-tailed t-tests or one-way ANOVA with Tukey’s post hoc test. *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: The ACLY inhibitor BMS-303141 (MedChemExpress; HY-16107) was dissolved in DMSO (10 mM stock) and used at 50 μM for 48 h. Vorinostat (MedChemExpress; HY-10221) was dissolved in DMSO and used at 0.5 μM (SW480) or 3.5 μM (DLD1) for 48 h. Sodium ascorbate (Sigma-Aldrich; A7632) was freshly prepared in sterile water (100 mM stock) and used at 5 mM for 6 h unless otherwise indicated.

Techniques: Activity Assay, Expressing, Western Blot, Transduction, Plasmid Preparation, Control, Two Tailed Test

Journal: Neoplasia (New York, N.Y.)

Article Title: Metabolic regulation of histone acetylation by ACLY supports MDR1 expression in colorectal cancer and highlights a targetable vulnerability

doi: 10.1016/j.neo.2026.101314

Figure Lengend Snippet: ACLY activity modulates histone acetylation and MDR1 expression. (A) Immunoblot analysis of MDR1 in SW480 and DLD1 cells treated with the histone deacetylase inhibitor vorinostat (VOR; 0.5 μM for SW480 and 3.5 μM for DLD1) or DMSO for 24 h. Representative blots and densitometric quantification relative to control are shown. (B) Immunoblot analysis of acetylated histone H3 (H3K9ac) and histone H4 (H4K16ac) in SW480 wild-type (WT) and ACLY-overexpressing (OE) cells treated with vehicle or the ACLY inhibitor BMS-303141 (50 μM, 48 h). (C) Immunoblot analysis of H3K9ac and H4K16ac in DLD1 cells under the same conditions. β-actin was used as a loading control. Data are presented as mean ± SD (n = 3). Statistical significance was determined using unpaired two-tailed t-tests. *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: The ACLY inhibitor BMS-303141 (MedChemExpress; HY-16107) was dissolved in DMSO (10 mM stock) and used at 50 μM for 48 h. Vorinostat (MedChemExpress; HY-10221) was dissolved in DMSO and used at 0.5 μM (SW480) or 3.5 μM (DLD1) for 48 h. Sodium ascorbate (Sigma-Aldrich; A7632) was freshly prepared in sterile water (100 mM stock) and used at 5 mM for 6 h unless otherwise indicated.

Techniques: Activity Assay, Expressing, Western Blot, Histone Deacetylase Assay, Control, Two Tailed Test

Journal: Neoplasia (New York, N.Y.)

Article Title: Metabolic regulation of histone acetylation by ACLY supports MDR1 expression in colorectal cancer and highlights a targetable vulnerability

doi: 10.1016/j.neo.2026.101314

Figure Lengend Snippet: ACLY expression is associated with resistance-related transcriptional programs in colorectal cancer. (A) Correlation analysis between ACLY expression and a gene set associated with lipid metabolism (ACLY, ACSS2, ACSS1, FASN, SREBP1) and drug transport pathways (ABCB1, ABCC2, ABCG5, EpCAM, CD24) in colorectal cancer samples using GEPIA2 (TCGA dataset). (B) Schematic representation of a proposed model linking ACLY-dependent acetyl-CoA production to histone acetylation and transcriptional regulation in CRC cells. (C) Relative mRNA expression of EpCAM, ABCC2, and CD24 in SW480 and DLD1 cells overexpressing ACLY compared with empty vector controls. (D) Relative mRNA expression of EpCAM, ABCC2, and CD24 in SW480 and DLD1 cells treated with the ACLY inhibitor BMS-303141 (50 μM) compared with vehicle-treated controls. Gene expression levels were determined by qPCR and normalized to ACTB. Data are presented as mean ± SEM (n = 3). Statistical significance was determined using unpaired two-tailed t-tests. *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: The ACLY inhibitor BMS-303141 (MedChemExpress; HY-16107) was dissolved in DMSO (10 mM stock) and used at 50 μM for 48 h. Vorinostat (MedChemExpress; HY-10221) was dissolved in DMSO and used at 0.5 μM (SW480) or 3.5 μM (DLD1) for 48 h. Sodium ascorbate (Sigma-Aldrich; A7632) was freshly prepared in sterile water (100 mM stock) and used at 5 mM for 6 h unless otherwise indicated.

Techniques: Expressing, Drug Transport Assay, Plasmid Preparation, Gene Expression, Two Tailed Test

Journal: Orthopaedic Journal of Sports Medicine

Article Title: Magnetic Resonance Imaging Assessment of Bridge-Enhanced ACL Restoration: An Imaging Atlas of Morphological Features and Healing Trajectories

doi: 10.1177/23259671261445974

Figure Lengend Snippet: Treated ACL healing from tibia to femur (Both panels: Sagittal PD MRI). (A) ACL healing in a knee with femoral tunnel close to the native attachment (anterior-posterior location: 18%). (B) ACL healing in a knee with femoral tunnel further anterior to the native attachment (anterior-posterior location: 40%). Femoral tunnels are highlighted with the white arrows. ACL, anterior cruciate ligament; MRI, magnetic resonance imaging; PD, proton density weighted.

Article Snippet: A functional ACL brace (CTi brace; OSSUR) or a simple hinged knee brace was recommended for 6 to 12 weeks, with the functional knee brace recommended for cutting and pivoting sports for 2 years after surgery.

Techniques: Magnetic Resonance Imaging

Journal: Orthopaedic Journal of Sports Medicine

Article Title: Magnetic Resonance Imaging Assessment of Bridge-Enhanced ACL Restoration: An Imaging Atlas of Morphological Features and Healing Trajectories

doi: 10.1177/23259671261445974

Figure Lengend Snippet: Violin plots indicating the longitudinal changes in ACL orientation, size, and normalized signal intensity from 6 to 24 months after surgery, and compared with the contralateral native ACL (CTRL). PD images were used to measure sagittal elevation angle, and CISS was used to assess tissue quality and size. Dash lines indicate the median and interquartile range. Significant P -values are shown. ACL, anterior cruciate ligament; CISS, constructive interference in steady state; CTRL, control/contralateral native ACL; PD, proton density weighted.

Article Snippet: A functional ACL brace (CTi brace; OSSUR) or a simple hinged knee brace was recommended for 6 to 12 weeks, with the functional knee brace recommended for cutting and pivoting sports for 2 years after surgery.

Techniques: Control

Journal: Orthopaedic Journal of Sports Medicine

Article Title: Magnetic Resonance Imaging Assessment of Bridge-Enhanced ACL Restoration: An Imaging Atlas of Morphological Features and Healing Trajectories

doi: 10.1177/23259671261445974

Figure Lengend Snippet: Six-, 12-, and 24-month postoperative images of a patient who underwent ACL restoration—(A-C) top panels: sagittal PD MRI, (D-F) bottom panels: sagittal T2 MRI—demonstrating above-average healing. Progressively lower signal in intact fibers is observed at all time points, including a near-normal ACL appearance at 24 months (white arrows). ACL, anterior cruciate ligament; MRI, magnetic resonance imaging; PD, proton density weighted.

Article Snippet: A functional ACL brace (CTi brace; OSSUR) or a simple hinged knee brace was recommended for 6 to 12 weeks, with the functional knee brace recommended for cutting and pivoting sports for 2 years after surgery.

Techniques: Magnetic Resonance Imaging

Journal: Orthopaedic Journal of Sports Medicine

Article Title: Magnetic Resonance Imaging Assessment of Bridge-Enhanced ACL Restoration: An Imaging Atlas of Morphological Features and Healing Trajectories

doi: 10.1177/23259671261445974

Figure Lengend Snippet: Six-, 12-, and 24-month postoperative images of a patient who underwent ACL restoration—(A-C) top panels: sagittal PD MRI, (D-F) bottom panels: sagittal T2 MRI—demonstrating average healing. Low-signal intact fibers are seen throughout all time points, including a near-normal ACL appearance at 24 months (white arrows). There is increased fluid signal intensity extending into Hoffa's fat pad (red arrows), indicating associated edema, which improved over time. ACL, anterior cruciate ligament; MRI, magnetic resonance imaging; PD, proton density weighted.

Article Snippet: A functional ACL brace (CTi brace; OSSUR) or a simple hinged knee brace was recommended for 6 to 12 weeks, with the functional knee brace recommended for cutting and pivoting sports for 2 years after surgery.

Techniques: Magnetic Resonance Imaging

Journal: Orthopaedic Journal of Sports Medicine

Article Title: Magnetic Resonance Imaging Assessment of Bridge-Enhanced ACL Restoration: An Imaging Atlas of Morphological Features and Healing Trajectories

doi: 10.1177/23259671261445974

Figure Lengend Snippet: Six-, 12-, and 24-month postoperative images of a patient who underwent ACL restoration—(A-C) top panels: sagittal PD MRI, (D-F) bottom panels: sagittal T2 MRI—demonstrating below-average healing. Despite associated edema extending into Hoffa's fat pad throughout (red arrows), the ACL has interval improvement with intact fibers from the femoral to tibial attachments (white arrows). ACL, anterior cruciate ligament; MRI, magnetic resonance imaging; PD, proton density weighted.

Article Snippet: A functional ACL brace (CTi brace; OSSUR) or a simple hinged knee brace was recommended for 6 to 12 weeks, with the functional knee brace recommended for cutting and pivoting sports for 2 years after surgery.

Techniques: Magnetic Resonance Imaging

Journal: Orthopaedic Journal of Sports Medicine

Article Title: Magnetic Resonance Imaging Assessment of Bridge-Enhanced ACL Restoration: An Imaging Atlas of Morphological Features and Healing Trajectories

doi: 10.1177/23259671261445974

Figure Lengend Snippet: Six-, 9-, and 12-month postoperative images of a patient who underwent ACL restoration and experienced a tear after a normal healing trajectory—(A-C) top panels: sagittal PD MRI, (D-F) bottom panels: sagittal T2 MRI. (A, D) Intact fibers are seen at 6 months, but (B, E) after an injurious event, there is greater signal heterogeneity, and intact fibers cannot be followed from the femoral to tibial attachments (white arrows). (C, F) At 12 months, the ACL had a notably more horizontal appearance than expected. ACL, anterior cruciate ligament; MRI, magnetic resonance imaging; PD, proton density weighted.

Article Snippet: A functional ACL brace (CTi brace; OSSUR) or a simple hinged knee brace was recommended for 6 to 12 weeks, with the functional knee brace recommended for cutting and pivoting sports for 2 years after surgery.

Techniques: Magnetic Resonance Imaging

Journal: Orthopaedic Journal of Sports Medicine

Article Title: Magnetic Resonance Imaging Assessment of Bridge-Enhanced ACL Restoration: An Imaging Atlas of Morphological Features and Healing Trajectories

doi: 10.1177/23259671261445974

Figure Lengend Snippet: Six-, 12-, and 14-month postoperative images of a patient who underwent ACL restoration and demonstrated poor healing—(A-C) top panel: sagittal PD MRI, (D-F) bottom panels: sagittal T2 MRI. There is increased fluid signal intensity noted in the intercondylar notch, indicating fibrosis, and no intact ACL fibers can be followed from the femoral to tibial attachments (white arrows). ACL, anterior cruciate ligament; MRI, magnetic resonance imaging; PD, proton density weighted.

Article Snippet: A functional ACL brace (CTi brace; OSSUR) or a simple hinged knee brace was recommended for 6 to 12 weeks, with the functional knee brace recommended for cutting and pivoting sports for 2 years after surgery.

Techniques: Magnetic Resonance Imaging

Journal: Orthopaedic Journal of Sports Medicine

Article Title: Magnetic Resonance Imaging Assessment of Bridge-Enhanced ACL Restoration: An Imaging Atlas of Morphological Features and Healing Trajectories

doi: 10.1177/23259671261445974

Figure Lengend Snippet: Twenty-four–month postoperative images of a patient who underwent ACL restoration and demonstrated a lack of femoral stump integration—(A) left panel: sagittal PD MRI, (B) right panel: sagittal T2 MRI. There is a clear separation (yellow arrows) between the ACL tissue connecting the tibia to the femoral tunnel (white arrows) and the ACL fibers coming from the native femoral insertion (red arrows). Despite the appearance of 2 separate strands, the patient did well clinically and did not require revision. ACL, anterior cruciate ligament; MRI, magnetic resonance imaging; PD, proton density weighted.

Article Snippet: A functional ACL brace (CTi brace; OSSUR) or a simple hinged knee brace was recommended for 6 to 12 weeks, with the functional knee brace recommended for cutting and pivoting sports for 2 years after surgery.

Techniques: Magnetic Resonance Imaging