Journal: Frontiers in Microbiology

Article Title: Fe biomineralization mirrors individual metabolic activity in a nitrate-dependent Fe(II)-oxidizer

doi: 10.3389/fmicb.2015.00879

Figure Lengend Snippet: Electron microscopy analysis of BoFeN1 cells cultured in the different biomineralization media: (A,B); Lp-, (C,D); Mt-, (E,F); FeP- and (G,H); Gt-media. (A,C,E,G) are TEM images and (B,D,F,H) are scanning electron microscopy (SEM) images in secondary electron mode. Insets display corresponding selected area electron diffraction (SAED) patterns of lepidocrocite (A) , magnetite (C) , amorphous Fe-phosphate (E) , and goethite (G) . White and orange arrows indicate non-encrusted vs. encrusted cells, respectively.

Article Snippet: This has been observed not only for Acidovorax sp. strain BoFeN1 but also for Pseudogulbenkania sp. strain 2002 and for heterotrophic nitrate reducers such as Paracoccus denitrificans ATCC 19367 and P. denitrificans Pd 1222 ( ).

Techniques: Electron Microscopy, Cell Culture

Journal: Frontiers in Microbiology

Article Title: Fe biomineralization mirrors individual metabolic activity in a nitrate-dependent Fe(II)-oxidizer

doi: 10.3389/fmicb.2015.00879

Figure Lengend Snippet: Co-existence of mineralized and non-mineralized cells in cultures of BoFeN1 collected from biomineralization media. (A–H): NanoSIMS images ( 12 C 14 N and 16 O) of BoFeN1 grown in biomineralization medium (Lp-, Mt-, FeP-, or Gt-media) for 4 days (t min = 4 days) then transferred to labeled medium for 4 h (t lab = 4 h). Scale bars: 2 μm.

Article Snippet: This has been observed not only for Acidovorax sp. strain BoFeN1 but also for Pseudogulbenkania sp. strain 2002 and for heterotrophic nitrate reducers such as Paracoccus denitrificans ATCC 19367 and P. denitrificans Pd 1222 ( ).

Techniques: Labeling

Journal: Frontiers in Microbiology

Article Title: Fe biomineralization mirrors individual metabolic activity in a nitrate-dependent Fe(II)-oxidizer

doi: 10.3389/fmicb.2015.00879

Figure Lengend Snippet: Differences in carbon assimilation among mineralized (orange arrows) and non-mineralized (white arrows) cells in cultures of BoFeN1 grown for 4 days (t min = 4 days) in biomineralization medium (Lp-, Mt-, FeP-, or Gt-media) then transferred to labeled medium for 4 days. NanoSIMS images of 12 C 14 N - signal (A,E,I,M) , 13 C 14 N/ 12 C 14 N ratio (B,F,J,N) and 16 O - signal (C,G,K,O) and corresponding X-EDS maps showing the distribution of Fe (green, D,H,L,P ). Scale bars: 2 μm.

Article Snippet: This has been observed not only for Acidovorax sp. strain BoFeN1 but also for Pseudogulbenkania sp. strain 2002 and for heterotrophic nitrate reducers such as Paracoccus denitrificans ATCC 19367 and P. denitrificans Pd 1222 ( ).

Techniques: Labeling