acid solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher acid solution
    Acid Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acid solution/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    acid solution - by Bioz Stars, 2022-01
    93/100 stars

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    Thermo Fisher mem nonessential amino acids
    SIM and LOV do not increase glycolysis. <t>HepG2</t> cells were treated with LOV (10 µM) or SIM (10 µM) for 3, 6, and 24 h. Cell-culture <t>medium</t> was collected and lactate was determined as described in Materials and Methods. Analysis of 3 independent experiments.
    Mem Nonessential Amino Acids, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Price from $9.99 to $1999.99
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    95
    Thermo Fisher syto82 orange fluorescent nucleic acid stain
    Experimental results. ( a ) Reaction constructed to measure the Δ G ° of a 5′ ROX fluorophore next to an adjacent DNA duplex with a terminal A nucleotide . ( b ) Fluorescent PAGE demonstrating non-covalent catalysis. Relevant species were reacted at 25 °C in 12.5 mM Mg 2+ buffer; 1x denotes ∼300 nM. Lanes 3 and 4 show significant difference in band intensity distribution, indicating that equilibrium is not achieved after 3 h of reaction in the absence of the catalyst. In contrast, lanes 5 and 6 show that a sub-stoichiometric (0.1x) amount of catalyst drives the reaction to equilibrium. Listed numbers below the gel are quantitated gel band intensities (arbitrary fluorescence units). ( c ) Summary of five additional experimental repeats. From the 12 inferred Δ G ° values, we obtain mean . ( d ) Experiments to measure at 10, 25, 37 and 45 °C in PBS (blue), as well as in 12.5 mM Mg 2+ (brown). The mean and mean s.d. of the Δ G ° values are plotted against temperature; error bars show 1 s.d. The gels were always run at the same temperature as the hybridization reaction. The green line shows the best linear fit against the four sets of experiments in PBS, corresponding to Δ H °=−0.81 kcal mol −1 and Δ S °=−1.89 cal mol −1 K −1 . ( e ) Melt curve of blunt duplex and 5′ ROX-labelled duplex in PBS buffer, based on fluorescence from the <t>Syto82</t> intercalating dye ( Supplementary Figs 70–72 and Supplementary Note 7 ). Upper and lower baselines are fitted to infer hybridization yields and equilibrium constants at different temperatures. ( f ) Van't Hoff plot of ln( K eq ) against (Kelvin). ( g ) Melt curve analysis produced a Δ G ° estimate with 14-fold higher s.d. than inferred using our native catalysis approach. Box shows 1 s.d. and error bars show 2 s.d.'s.
    Syto82 Orange Fluorescent Nucleic Acid Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher syto 85
    Loss of Snx14 perturbs LD size and morphology but does not change neutral lipid levels. (A) Confocal micrographs of WT and SNX14 KO cells treated with OA overnight. LDs visualized by MDH (black) and nucleus stained with <t>Syto</t> 85 orange fluorescent stain (blue outline). Images were processed so that LDs were converted to grayscale and inverted. Scale bar = 25 µm. (B) Quantification of average area covered by LDs per cell of representative images from A. Total LD area was derived from more than five fields of view, each consisting of approximately five cells or more of two different sets of experiments (total no. of cells > 75; ***, P
    Syto 85, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher syto13
    (a) and (b) Subcellular localization of CPT (blue) in live BT-474 breast cancer cells after 2 h CPT- Alexa 594 TTZ nanoparticle exposure followed by 24 h incubation in cell culture medium at 37°C. The cell nuclei were stained with green <t>SYTO13</t>
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    SIM and LOV do not increase glycolysis. HepG2 cells were treated with LOV (10 µM) or SIM (10 µM) for 3, 6, and 24 h. Cell-culture medium was collected and lactate was determined as described in Materials and Methods. Analysis of 3 independent experiments.

    Journal: The FASEB Journal

    Article Title: Statin-dependent modulation of mitochondrial metabolism in cancer cells is independent of cholesterol content

    doi: 10.1096/fj.201802723R

    Figure Lengend Snippet: SIM and LOV do not increase glycolysis. HepG2 cells were treated with LOV (10 µM) or SIM (10 µM) for 3, 6, and 24 h. Cell-culture medium was collected and lactate was determined as described in Materials and Methods. Analysis of 3 independent experiments.

    Article Snippet: Huh7 cells were grown in the same growth medium as HepG2 with the addition of 1% 100× MEM nonessential amino acids (11140-50; Thermo Fisher Scientific).

    Techniques: Cell Culture

    SIM does not change NADH. HepG2 cells treated with SIM (10 µM) for 3, 6, and 24 h were grown in whole medium and imaged in HBSS. A ) NADH autofluorescence after SIM was assessed by multiphoton confocal microscopy as described in Materials and Methods. B , D ) CCCP + oligo and Myxo were used as controls of NADH autofluorescence. C ) Quantitative analysis of relative NADH autofluorescence from 3 independent experiments. Four to 5 randomly selected fields with 10–20 cells per field were analyzed. A.u., arbitrary units. * P

    Journal: The FASEB Journal

    Article Title: Statin-dependent modulation of mitochondrial metabolism in cancer cells is independent of cholesterol content

    doi: 10.1096/fj.201802723R

    Figure Lengend Snippet: SIM does not change NADH. HepG2 cells treated with SIM (10 µM) for 3, 6, and 24 h were grown in whole medium and imaged in HBSS. A ) NADH autofluorescence after SIM was assessed by multiphoton confocal microscopy as described in Materials and Methods. B , D ) CCCP + oligo and Myxo were used as controls of NADH autofluorescence. C ) Quantitative analysis of relative NADH autofluorescence from 3 independent experiments. Four to 5 randomly selected fields with 10–20 cells per field were analyzed. A.u., arbitrary units. * P

    Article Snippet: Huh7 cells were grown in the same growth medium as HepG2 with the addition of 1% 100× MEM nonessential amino acids (11140-50; Thermo Fisher Scientific).

    Techniques: Confocal Microscopy

    Experimental results. ( a ) Reaction constructed to measure the Δ G ° of a 5′ ROX fluorophore next to an adjacent DNA duplex with a terminal A nucleotide . ( b ) Fluorescent PAGE demonstrating non-covalent catalysis. Relevant species were reacted at 25 °C in 12.5 mM Mg 2+ buffer; 1x denotes ∼300 nM. Lanes 3 and 4 show significant difference in band intensity distribution, indicating that equilibrium is not achieved after 3 h of reaction in the absence of the catalyst. In contrast, lanes 5 and 6 show that a sub-stoichiometric (0.1x) amount of catalyst drives the reaction to equilibrium. Listed numbers below the gel are quantitated gel band intensities (arbitrary fluorescence units). ( c ) Summary of five additional experimental repeats. From the 12 inferred Δ G ° values, we obtain mean . ( d ) Experiments to measure at 10, 25, 37 and 45 °C in PBS (blue), as well as in 12.5 mM Mg 2+ (brown). The mean and mean s.d. of the Δ G ° values are plotted against temperature; error bars show 1 s.d. The gels were always run at the same temperature as the hybridization reaction. The green line shows the best linear fit against the four sets of experiments in PBS, corresponding to Δ H °=−0.81 kcal mol −1 and Δ S °=−1.89 cal mol −1 K −1 . ( e ) Melt curve of blunt duplex and 5′ ROX-labelled duplex in PBS buffer, based on fluorescence from the Syto82 intercalating dye ( Supplementary Figs 70–72 and Supplementary Note 7 ). Upper and lower baselines are fitted to infer hybridization yields and equilibrium constants at different temperatures. ( f ) Van't Hoff plot of ln( K eq ) against (Kelvin). ( g ) Melt curve analysis produced a Δ G ° estimate with 14-fold higher s.d. than inferred using our native catalysis approach. Box shows 1 s.d. and error bars show 2 s.d.'s.

    Journal: Nature Communications

    Article Title: Native characterization of nucleic acid motif thermodynamics via non-covalent catalysis

    doi: 10.1038/ncomms10319

    Figure Lengend Snippet: Experimental results. ( a ) Reaction constructed to measure the Δ G ° of a 5′ ROX fluorophore next to an adjacent DNA duplex with a terminal A nucleotide . ( b ) Fluorescent PAGE demonstrating non-covalent catalysis. Relevant species were reacted at 25 °C in 12.5 mM Mg 2+ buffer; 1x denotes ∼300 nM. Lanes 3 and 4 show significant difference in band intensity distribution, indicating that equilibrium is not achieved after 3 h of reaction in the absence of the catalyst. In contrast, lanes 5 and 6 show that a sub-stoichiometric (0.1x) amount of catalyst drives the reaction to equilibrium. Listed numbers below the gel are quantitated gel band intensities (arbitrary fluorescence units). ( c ) Summary of five additional experimental repeats. From the 12 inferred Δ G ° values, we obtain mean . ( d ) Experiments to measure at 10, 25, 37 and 45 °C in PBS (blue), as well as in 12.5 mM Mg 2+ (brown). The mean and mean s.d. of the Δ G ° values are plotted against temperature; error bars show 1 s.d. The gels were always run at the same temperature as the hybridization reaction. The green line shows the best linear fit against the four sets of experiments in PBS, corresponding to Δ H °=−0.81 kcal mol −1 and Δ S °=−1.89 cal mol −1 K −1 . ( e ) Melt curve of blunt duplex and 5′ ROX-labelled duplex in PBS buffer, based on fluorescence from the Syto82 intercalating dye ( Supplementary Figs 70–72 and Supplementary Note 7 ). Upper and lower baselines are fitted to infer hybridization yields and equilibrium constants at different temperatures. ( f ) Van't Hoff plot of ln( K eq ) against (Kelvin). ( g ) Melt curve analysis produced a Δ G ° estimate with 14-fold higher s.d. than inferred using our native catalysis approach. Box shows 1 s.d. and error bars show 2 s.d.'s.

    Article Snippet: SYTO82 orange fluorescent nucleic acid stain was supplied as 5 mM solution in DMSO from Life technologies.

    Techniques: Construct, Polyacrylamide Gel Electrophoresis, Fluorescence, Hybridization, Produced

    Loss of Snx14 perturbs LD size and morphology but does not change neutral lipid levels. (A) Confocal micrographs of WT and SNX14 KO cells treated with OA overnight. LDs visualized by MDH (black) and nucleus stained with Syto 85 orange fluorescent stain (blue outline). Images were processed so that LDs were converted to grayscale and inverted. Scale bar = 25 µm. (B) Quantification of average area covered by LDs per cell of representative images from A. Total LD area was derived from more than five fields of view, each consisting of approximately five cells or more of two different sets of experiments (total no. of cells > 75; ***, P

    Journal: The Journal of Cell Biology

    Article Title: Cerebellar ataxia disease–associated Snx14 promotes lipid droplet growth at ER–droplet contacts

    doi: 10.1083/jcb.201808133

    Figure Lengend Snippet: Loss of Snx14 perturbs LD size and morphology but does not change neutral lipid levels. (A) Confocal micrographs of WT and SNX14 KO cells treated with OA overnight. LDs visualized by MDH (black) and nucleus stained with Syto 85 orange fluorescent stain (blue outline). Images were processed so that LDs were converted to grayscale and inverted. Scale bar = 25 µm. (B) Quantification of average area covered by LDs per cell of representative images from A. Total LD area was derived from more than five fields of view, each consisting of approximately five cells or more of two different sets of experiments (total no. of cells > 75; ***, P

    Article Snippet: LDs were visualized by staining the cells with MDH (1:1,000; Abgent; SM1000a) for 15 min. Nucleus was stained with 5 μM Syto 85 orange fluorescent stain (Molecular Probes; S-11366).

    Techniques: Staining, Derivative Assay

    (a) and (b) Subcellular localization of CPT (blue) in live BT-474 breast cancer cells after 2 h CPT- Alexa 594 TTZ nanoparticle exposure followed by 24 h incubation in cell culture medium at 37°C. The cell nuclei were stained with green SYTO13

    Journal: ACS nano

    Article Title: Synergistic Targeting of Cell Membrane, Cytoplasm and Nucleus of Cancer Cells using Rod-Shaped Nanoparticles

    doi: 10.1021/nn403913k

    Figure Lengend Snippet: (a) and (b) Subcellular localization of CPT (blue) in live BT-474 breast cancer cells after 2 h CPT- Alexa 594 TTZ nanoparticle exposure followed by 24 h incubation in cell culture medium at 37°C. The cell nuclei were stained with green SYTO13

    Article Snippet: To stain the cell nuclei, cells were incubated with 1µM green fluorescent nuclear stain, SYTO13 (Molecular Probes) for 1 h before washing.

    Techniques: Cycling Probe Technology, Incubation, Cell Culture, Staining