Journal: Cell metabolism

Article Title: Dietary Sugars Alter Hepatic Fatty Acid Oxidation via Transcriptional and Post-translational Modifications of Mitochondrial Proteins

doi: 10.1016/j.cmet.2019.09.003

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: PTMScan Acety-Lysine Motif [Ac-K] Kit, Immunoaffinity Beads , Cell Signaling Technology , 13416.

Techniques: Recombinant, Protease Inhibitor, Sequencing, Modification, Enzyme-linked Immunosorbent Assay, RNA Sequencing Assay, Mass Spectrometry, Software

Journal: Journal of proteome research

Article Title: Quantifying Competition Among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism

doi: 10.1021/acs.jproteome.8b00800

Figure Lengend Snippet: Ethanol increases lysine acetylation and decreases succinylation of mitochondrial proteins in WT, SIRT3 KO, and SIRT5 KO mouse livers. (A) Representative anti-acetyllysine Western blot. (B) Representative anti-succinyllysine Western blot. (C) Representative SIRT3, SIRT5, and loading control (COXIV) Western blots. (D-G) Corresponding densitometry quantification using unpaired Student’s t-test (mean ± SEM, n=3, *=p<0.05, **=p<0.005, ***=p<0.0005, ****=p<0.0001).

Article Snippet: Samples were incubated 2 hours at 4 °C with immunoaffinity beads conjugated to acetyllysine antibody or immunoaffinity beads conjugated to succinyllysine antibody (PTMScan® Acetyl-Lysine Motif [Ac-K] Immunoaffinity Beads #13416 and PTMScan® Succinyl-Lysine Motif [Succ-K] Immunoaffinity Beads #13764, Cell Signaling, Boston, MA).

Techniques: Western Blot

Journal: Journal of proteome research

Article Title: Quantifying Competition Among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism

doi: 10.1021/acs.jproteome.8b00800

Figure Lengend Snippet: Ethanol metabolism alters hepatic protein acylation and lipid deposition in WT, SIRT3 KO, and SIRT5 KO mice. Paraffin embedded formalin fixed hepatic tissue sections were analyzed using (A) hematoxylin and eosin (H&E) staining and IHC for (B) Plin2, (C) acetyllysine, and (D) succinyllysine. [CV = central vein, PT= portal vein. (10× mag), (n=3)] See also Figures S1–S3.

Article Snippet: Samples were incubated 2 hours at 4 °C with immunoaffinity beads conjugated to acetyllysine antibody or immunoaffinity beads conjugated to succinyllysine antibody (PTMScan® Acetyl-Lysine Motif [Ac-K] Immunoaffinity Beads #13416 and PTMScan® Succinyl-Lysine Motif [Succ-K] Immunoaffinity Beads #13764, Cell Signaling, Boston, MA).

Techniques: Staining