Journal: Nutrients

Article Title: Novel Therapeutic Potentials of Taxifolin for Obesity-Induced Hepatic Steatosis, Fibrogenesis, and Tumorigenesis

doi: 10.3390/nu15020350

Figure Lengend Snippet: Preventive effects of Taxifolin on hepatic steatosis in diet-induced obese mice. White square: SD; black square: HD; dark-green square: TX-L; light-green square: TX-H. n = 6 in each group. ( A ) Serum concentrations of AST and ALT after 12 weeks of HD feeding; ( B , C ) hepatic triglyceride and MDA contents; ( D ) hematoxylin and eosin (HE) staining of the liver. Insets: gross appearance of the livers. Scale bars: 100 µm. ( E , F ) Expression levels of genes related to lipogenesis ( Srebp1c , Fas , Scd1 , and Acc1 ) and inflammation ( Tnfα , Il1b , and Emr1 (F4/80)) in the liver; ( G , H ) immunoblot analysis of the protein expression levels related to lipogenesis (FAS, SCD-1, and ACC) and inflammation (TNFα) in the liver. β-actin was used as a loading control. Values are presented as the means ± SEM; n = 6; significant differences: ** p < 0.01 vs. HD.

Article Snippet: The membrane was blocked with a blocking solution (Nacalai Tesque), followed by incubation with the following primary antibodies: anti-FAS (diluted 1:1000; #3180; Cell Signaling Technology, CST), anti-ACC (diluted 1:1000; #3676; CST), anti-SCD-1 (diluted 1:1000; #2794; CST), anti-TNFα (diluted 1:800; #11948; CST), and anti-β-actin (diluted 1:3000; #4980; CST).

Techniques: Staining, Expressing, Western Blot

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Targeted Delivery of Stk25 Antisense Oligonucleotides to Hepatocytes Protects Mice Against Nonalcoholic Fatty Liver Disease

doi: 10.1016/j.jcmgh.2018.12.004

Figure Lengend Snippet: Downstream targets and upstream activators of STK25. ( A ) Liver protein lysates from high-fat–fed mice treated with GalNAc- Stk25 ASO vs GalNAc-control ASO (12.5 mg/kg/wk) were analyzed by Western blot using antibodies specific for total ACC or phospho-ACC (Ser79), with pan-actin used as a loading control. ( B and C ) Liver lysates were collected from male C57BL/6J mice ( B ) fed a normal chow diet and fasted for 12 hours vs re-fed or ( C ) fed a normal chow diet vs a high-fat diet for 18 weeks. The lysates were immunoprecipitated with anti-STK25 antibody and analyzed by Western blot using antibodies specific for total STK25 or phospho-threonine. Immunoprecipitation of liver lysates from Stk25 knockout mice was included as a negative control. Protein levels were analyzed by densitometry and data are shown as the total and phospho-protein abundance, as well as the ratio of phospho-protein to total protein. Representative Western blots are shown. Data are means ± SD from 6–8 mice per group. Cntr, control. * P < .05.

Article Snippet: The detection of specific proteins used the following primary antibodies: anti-STK25, anti-PCNA, anti-Ki67, anti-ACC (#3662; Cell Signaling Technology, Boston, MA), anti–phospho-ACC (Ser79; #3661; Cell Signaling Technology), anti–phospho-threonine (13-9200; Invitrogen), anti–pan-actin (sc-8432; Santa Cruz Biotechnology), anti–glyceraldehyde-3-phosphate-dehydrogenase (MA5-15738; Invitrogen), anti–β-actin (ab8226; Abcam), and horseradish-peroxidase–conjugated secondary antibodies anti-rabbit IgG (#7074; Cell Signaling Technology), anti-mouse IgG (#7076; Cell Signaling Technology), anti-rat IgG (#7077; Cell signaling Technology), and VeriBlot for IP Detection Reagent (ab131366; Abcam).

Techniques: Western Blot, Immunoprecipitation, Knock-Out, Negative Control