acetonitrile acn  (Thermo Fisher)


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  • 99
    Name:
    Acetonitrile in Water 20
    Description:
    This reagent is intended for N terminal protein sequencing applications Use with other protein sequencing reagents 20 Acetonitrile in Water S4B 200 mL Procise Sequencing System Model 492 and 294 5 PITC in N Heptane 40 mL 25 Trifluoroacetic Acid in Water 40 mL Ethyl Acetate 450 mL N Butyl Chloride 200 mL Solvent A3 3 5 THF⁄Water 960 mL Solvent B2 Isopropanol and Acetonitrile 1 L For Research Use Only Not for use in diagnostics procedures
    Catalog Number:
    400314
    Price:
    None
    Applications:
    Protein Biology|Protein Sequencing|Protein Sequencing & Peptide Synthesis
    Category:
    Lab Reagents and Chemicals
    Buy from Supplier


    Structured Review

    Thermo Fisher acetonitrile acn
    MALDI mass spectra of purified rice-derived rhTF (a close-up view). The apo- rhTFs was desalted using ZipTip ™ <t>μ-C18</t> pipette tips (Millipore, Billerica, MA), eluted with a solution of 70% acetonitrile <t>(ACN),</t> 0.2% formic acid, and 5 mg/ml MALDI matrix (α-cyano-4-hydroxycinnamic acid), and analyzed with an Applied Biosystems 4700 Proteomics Analyzer.
    This reagent is intended for N terminal protein sequencing applications Use with other protein sequencing reagents 20 Acetonitrile in Water S4B 200 mL Procise Sequencing System Model 492 and 294 5 PITC in N Heptane 40 mL 25 Trifluoroacetic Acid in Water 40 mL Ethyl Acetate 450 mL N Butyl Chloride 200 mL Solvent A3 3 5 THF⁄Water 960 mL Solvent B2 Isopropanol and Acetonitrile 1 L For Research Use Only Not for use in diagnostics procedures
    https://www.bioz.com/result/acetonitrile acn/product/Thermo Fisher
    Average 99 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    acetonitrile acn - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Expression, purification, and characterization of recombinant human transferrin from rice (Oryza sativa L.)"

    Article Title: Expression, purification, and characterization of recombinant human transferrin from rice (Oryza sativa L.)

    Journal: Protein expression and purification

    doi: 10.1016/j.pep.2010.04.019

    MALDI mass spectra of purified rice-derived rhTF (a close-up view). The apo- rhTFs was desalted using ZipTip ™ μ-C18 pipette tips (Millipore, Billerica, MA), eluted with a solution of 70% acetonitrile (ACN), 0.2% formic acid, and 5 mg/ml MALDI matrix (α-cyano-4-hydroxycinnamic acid), and analyzed with an Applied Biosystems 4700 Proteomics Analyzer.
    Figure Legend Snippet: MALDI mass spectra of purified rice-derived rhTF (a close-up view). The apo- rhTFs was desalted using ZipTip ™ μ-C18 pipette tips (Millipore, Billerica, MA), eluted with a solution of 70% acetonitrile (ACN), 0.2% formic acid, and 5 mg/ml MALDI matrix (α-cyano-4-hydroxycinnamic acid), and analyzed with an Applied Biosystems 4700 Proteomics Analyzer.

    Techniques Used: Purification, Derivative Assay, Transferring

    2) Product Images from "Anti-Inflammatory and Antinociceptive Properties of Flavonoids from the Fruits of Black Mulberry (Morus nigra L.)"

    Article Title: Anti-Inflammatory and Antinociceptive Properties of Flavonoids from the Fruits of Black Mulberry (Morus nigra L.)

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0153080

    Chromatograms of rutin (A) and anthocyanins (B) of TF by UPLC-TUV. Rutin and anthocyanins were separated on the same Acquity UPLC BEH C18 column (2.1x100 mm, 1.7 μm, Waters, Milford, MA). Rutin separation used MeOH and 0.1% H 3 PO 4 as the mobile phase at 0.21 mL/min with detection at 358 nm. Anthocyanins separation used ACN and 0.2% H 3 PO 4 as the mobile phase at 0.3 mL/min and detection at 520 nm. Rutin: quercetin-3-O-rutinlside; C3G: cyanidin-3-O-glucoside; C3R: cyanidin-3-O-rutinoside; and TF: total flavonoids.
    Figure Legend Snippet: Chromatograms of rutin (A) and anthocyanins (B) of TF by UPLC-TUV. Rutin and anthocyanins were separated on the same Acquity UPLC BEH C18 column (2.1x100 mm, 1.7 μm, Waters, Milford, MA). Rutin separation used MeOH and 0.1% H 3 PO 4 as the mobile phase at 0.21 mL/min with detection at 358 nm. Anthocyanins separation used ACN and 0.2% H 3 PO 4 as the mobile phase at 0.3 mL/min and detection at 520 nm. Rutin: quercetin-3-O-rutinlside; C3G: cyanidin-3-O-glucoside; C3R: cyanidin-3-O-rutinoside; and TF: total flavonoids.

    Techniques Used:

    3) Product Images from "Unsymmetrical Spiroalkanedithiols Having Mixed Fluorinated and Alkyl Tailgroups of Varying Length: Film Structure and Interfacial Properties"

    Article Title: Unsymmetrical Spiroalkanedithiols Having Mixed Fluorinated and Alkyl Tailgroups of Varying Length: Film Structure and Interfacial Properties

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    doi: 10.3390/molecules23102632

    Advancing contact angle data obtained for water (H 2 O), N , N -dimethylformamide (DMF), acetonitrile (ACN), hexadecane (HD), and perfluorodecalin (PFD) on SAMs derived from the monodentate thiols ( C10SH , C12SH , C18SH , and F8H10SH ) and the F8H10-Cm dithiols ( F8H10-C10 , F8H10-C12 , and F8H10-C18 ).
    Figure Legend Snippet: Advancing contact angle data obtained for water (H 2 O), N , N -dimethylformamide (DMF), acetonitrile (ACN), hexadecane (HD), and perfluorodecalin (PFD) on SAMs derived from the monodentate thiols ( C10SH , C12SH , C18SH , and F8H10SH ) and the F8H10-Cm dithiols ( F8H10-C10 , F8H10-C12 , and F8H10-C18 ).

    Techniques Used: Derivative Assay

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: In Vitro Metabolites of Di-2-ethylhexyl Adipate (DEHA) as Biomarkers of Exposure in Human Biomonitoring Applications
    Article Snippet: .. Details on the HPLC gradient for the separation of DEHA and DEHP metabolites and the method for online SPE are presented elsewhere., Briefly, metabolites in the supernatant of the human liver microsomal homogenate (500 μ L), obtained after incubating for 5 h with DEHA and MEHA, were extracted using online SPE on a Chromolith RP-18 precolumn (Merck KGaA, Darmstadt, Germany), resolved on a Betasil phenyl HPLC column (3 μ M, 2.1 mm × 25 mm, ThermoFisher Scientific, San Jose, CA, USA) using a water/acetonitrile gradient program and detected by mass spectrometry on a TSQ Vantage AM triple quadrupole mass spectrometer (ThermoFisher Scientific, San Jose, CA, USA). ..

    Article Title: Measurement of DNA concentration as a normalization strategy for metabolomic data from adherent cell lines
    Article Snippet: .. HPLC-grade water (H2 O) and acetonitrile (ACN) were purchased from Thermo Fisher Scientific (Pittsburgh, PA, USA). .. To determine the accuracy of total protein measurement, serial dilutions of 0.25, 0.5, 1, 2, and 4 million OVCAR-8 cells were seeded in 10 cm dishes (Corning, Tewksbury, MA) in triplicate.

    Transferring:

    Article Title: Expression, purification, and characterization of recombinant human transferrin from rice (Oryza sativa L.)
    Article Snippet: .. These iron-free or apo-TFs were further desalted using ZipTip™ μ-C18 pipette tips (Millipore, Billerica, MA), eluted with a solution of 70% acetonitrile (ACN), 0.2% formic acid, and 5 mg/ml MALDI matrix (α-cyano-4-hydroxycinnamic acid), and spotted onto the MALDI target and analyzed with an Applied Biosystems 4700 Proteomics Analyzer (Applied Biosystems Inc., Foster City, CA) at the Molecular Structure Facility at the University of California, Davis, CA, US. .. To evaluate the glycosylation status of rice-derived rhTF, the purified rhTF protein was subjected to digestion with peptide-N-glycosidase F (PNGase F) (Sigma, St. Louis, MO).

    other:

    Article Title: Novel therapeutic targets to mitigate early neuronal dysfunction and cognitive deficits in tauopathy
    Article Snippet: Each sample fraction was loaded onto an Acclaim Pepmap 100 pre-column (20 mm × 75 μm; 3 μm-C18) and separated on a PepMap RSLC analytical column (250 mm × 75 μm; 2 μm-C18) at a flow rate at 350 nl/min during a linear gradient from solvent A (0.1% formic acid (v/v)) to 30% solvent B (0.1% formic acid (v/v) and 99.9% acetonitrile (v/v)) for 95 min, to 98% solvent B for 15 min, and hold 98% solvent B for additional 30 min. Full MS scans were acquired in the Orbitrap mass analyzer over m/z 400–2000 range with resolution 70,000 at 200 m/z.

    Mass Spectrometry:

    Article Title: In Vitro Metabolites of Di-2-ethylhexyl Adipate (DEHA) as Biomarkers of Exposure in Human Biomonitoring Applications
    Article Snippet: .. Details on the HPLC gradient for the separation of DEHA and DEHP metabolites and the method for online SPE are presented elsewhere., Briefly, metabolites in the supernatant of the human liver microsomal homogenate (500 μ L), obtained after incubating for 5 h with DEHA and MEHA, were extracted using online SPE on a Chromolith RP-18 precolumn (Merck KGaA, Darmstadt, Germany), resolved on a Betasil phenyl HPLC column (3 μ M, 2.1 mm × 25 mm, ThermoFisher Scientific, San Jose, CA, USA) using a water/acetonitrile gradient program and detected by mass spectrometry on a TSQ Vantage AM triple quadrupole mass spectrometer (ThermoFisher Scientific, San Jose, CA, USA). ..

    Article Title: Calcium-binding protein S100A4 confers mesenchymal progenitor cell fibrogenicity in idiopathic pulmonary fibrosis
    Article Snippet: .. Peptide samples were resuspended in 98:2 water/acetonitrile, 0.1% formic acid and run on an Orbitrap Velos (Thermo Fisher Scientific) mass spectrometer. .. The Thermo RAW files were analyzed with Peaks Studio 7.0 build 20140912 (Bioinformatics Solutions) software package for interpretation of tandem mass spectometry and protein inference.

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  • 99
    Thermo Fisher acetonitrile acn
    Chromatograms of rutin (A) and anthocyanins (B) of TF by UPLC-TUV. Rutin and anthocyanins were separated on the same Acquity UPLC BEH C18 column (2.1x100 mm, 1.7 μm, Waters, Milford, MA). Rutin separation used <t>MeOH</t> and 0.1% H 3 PO 4 as the mobile phase at 0.21 mL/min with detection at 358 nm. Anthocyanins separation used <t>ACN</t> and 0.2% H 3 PO 4 as the mobile phase at 0.3 mL/min and detection at 520 nm. Rutin: quercetin-3-O-rutinlside; C3G: cyanidin-3-O-glucoside; C3R: cyanidin-3-O-rutinoside; and TF: total flavonoids.
    Acetonitrile Acn, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acetonitrile acn/product/Thermo Fisher
    Average 99 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    acetonitrile acn - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher hplc grade acetonitrile acn
    Chromatograms of rutin (A) and anthocyanins (B) of TF by UPLC-TUV. Rutin and anthocyanins were separated on the same Acquity UPLC BEH C18 column (2.1x100 mm, 1.7 μm, Waters, Milford, MA). Rutin separation used <t>MeOH</t> and 0.1% H 3 PO 4 as the mobile phase at 0.21 mL/min with detection at 358 nm. Anthocyanins separation used <t>ACN</t> and 0.2% H 3 PO 4 as the mobile phase at 0.3 mL/min and detection at 520 nm. Rutin: quercetin-3-O-rutinlside; C3G: cyanidin-3-O-glucoside; C3R: cyanidin-3-O-rutinoside; and TF: total flavonoids.
    Hplc Grade Acetonitrile Acn, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc grade acetonitrile acn/product/Thermo Fisher
    Average 92 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    hplc grade acetonitrile acn - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    Chromatograms of rutin (A) and anthocyanins (B) of TF by UPLC-TUV. Rutin and anthocyanins were separated on the same Acquity UPLC BEH C18 column (2.1x100 mm, 1.7 μm, Waters, Milford, MA). Rutin separation used MeOH and 0.1% H 3 PO 4 as the mobile phase at 0.21 mL/min with detection at 358 nm. Anthocyanins separation used ACN and 0.2% H 3 PO 4 as the mobile phase at 0.3 mL/min and detection at 520 nm. Rutin: quercetin-3-O-rutinlside; C3G: cyanidin-3-O-glucoside; C3R: cyanidin-3-O-rutinoside; and TF: total flavonoids.

    Journal: PLoS ONE

    Article Title: Anti-Inflammatory and Antinociceptive Properties of Flavonoids from the Fruits of Black Mulberry (Morus nigra L.)

    doi: 10.1371/journal.pone.0153080

    Figure Lengend Snippet: Chromatograms of rutin (A) and anthocyanins (B) of TF by UPLC-TUV. Rutin and anthocyanins were separated on the same Acquity UPLC BEH C18 column (2.1x100 mm, 1.7 μm, Waters, Milford, MA). Rutin separation used MeOH and 0.1% H 3 PO 4 as the mobile phase at 0.21 mL/min with detection at 358 nm. Anthocyanins separation used ACN and 0.2% H 3 PO 4 as the mobile phase at 0.3 mL/min and detection at 520 nm. Rutin: quercetin-3-O-rutinlside; C3G: cyanidin-3-O-glucoside; C3R: cyanidin-3-O-rutinoside; and TF: total flavonoids.

    Article Snippet: Acetonitrile (ACN) and methanol (MeOH) for UPLC analysis were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: