jnk inhibitor 1 3 benzothiazol 2 yl 2 2 3 pyridinyl ethyl amino 4 pyrimidinyl acetonitrile  (Millipore)


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    Structured Review

    Millipore jnk inhibitor 1 3 benzothiazol 2 yl 2 2 3 pyridinyl ethyl amino 4 pyrimidinyl acetonitrile
    Jnk Inhibitor 1 3 Benzothiazol 2 Yl 2 2 3 Pyridinyl Ethyl Amino 4 Pyrimidinyl Acetonitrile, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jnk inhibitor 1 3 benzothiazol 2 yl 2 2 3 pyridinyl ethyl amino 4 pyrimidinyl acetonitrile/product/Millipore
    Average 77 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    jnk inhibitor 1 3 benzothiazol 2 yl 2 2 3 pyridinyl ethyl amino 4 pyrimidinyl acetonitrile - by Bioz Stars, 2020-01
    77/100 stars

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    Related Articles

    Negative Control:

    Article Title: cAMP-guanine exchange factor protection from bile acid-induced hepatocyte apoptosis involves glycogen synthase kinase regulation of c-Jun NH2-terminal kinase
    Article Snippet: The GSK inhibitors lithium chloride (LiCl), 3-[(3-chloro-4-hydroxyphenyl)amino]-4-(2-nitrophenyl)-1H-pyrrol-2,5-dione (SB415286), and 3-(2,4-dichlorophenyl)-4-(1-methyl-1H-indol-3-yl)-1H-pyrrole-2,5-dione (SB216763) were from Sigma Aldrich Chemical. .. Additional GSK inhibitors 4-benzyl-2-methyl-1,2,4-thiadiazolidine-3,5-dione (TDZD), (2′Z,3′E)-6-bromoindirubin-3′-oxime (BIO), its negative control compound 1-methyl-BIO (ME-BIO), the Src inhibitor 4-amino-5-(4-chlorophenyl)-7-( t -butyl)pyrazolo[3,4-d]pyrimidine (PP2), and its inactive control 4-amino-7-phenylpyrazol [3,4-d]pyrimidine (PP3), the JNK inhibitor 1,3-benzothiazol-2-yl-(2-((2-(3 pyridinyl)ethyl)amino)-4-pyrimidinyl)acetonitrile (AS601645), and antibodies to actin were from EMD Bioscience (San Diego, CA). .. The cAMP-GEF-specific cAMP analog 4-(4-chlorophenylthio)-2′- O -methyladenosine-3′,5′-cyclic monophosphate (CPT-2-Me-cAMP), the specific PKA inhibitor Rp-8-(4-chlorophenylthio)-cAMP (Rp-CPT-cAMP), and Fas ligand were from Axxora (San Diego, CA).

    Article Title: The contribution of c-Jun N-terminal kinase activation and subsequent Bcl-2 phosphorylation to apoptosis induction in human B-cells is dependent on the mode of action of specific stresses
    Article Snippet: The antibody that recognizes the 113 kD full length poly (ADP ribose) polymerase (PARP) and the 85 kD cleavage product (PARP; AAP-250) and the antibody for Hsp 70 (SPA 812) were purchased from Stressgen, Inc. Antibodies for c-Jun (sc1694) and total Bcl-2 (sc509) were obtained from Santa Cruz. .. The JNK inhibitor SP600125 (SP6; Anthra[1,9- cd ]pyrazol-6(2 H )-one-1,9-pyrazoloanthron), JNK inhibitor II (a structural but inactive analogue of SP6 serving as a negative control; N1-methyl-1,9,pyrazoloanthrone), JNK inhibitor V, (a structurally unrelated JNK inhibitor, 1,3,-benzothiazole-2-yl-(2-(2-(3-pyridinyl)ethyl)amino)-4-pyrimidinyl acetonitrile), the ERK inhibitor U0126, and p38 inhibitor SB202190 (SB2), were obtained from Calbiochem Inc. All inhibitors were dissolved in DMSO. .. We previously performed experiments to optimize conditions of exposure of EW36 cells to SP6, U0126, and SB2 ( ; ; ) and found our conditions consistent with those reported in the literature.

    Cycling Probe Technology:

    Article Title: cAMP-guanine exchange factor protection from bile acid-induced hepatocyte apoptosis involves glycogen synthase kinase regulation of c-Jun NH2-terminal kinase
    Article Snippet: Collagenase, Hoechst 33258, glycochenodeoxycholate (GCDC), wortmannin, , 8-(4-chlorophenylthio)-cAMP (CPT-cAMP), tunicamycin, and all tissue culture reagents were purchased from Sigma-Aldrich Chemical (St. Louis, MO). .. Additional GSK inhibitors 4-benzyl-2-methyl-1,2,4-thiadiazolidine-3,5-dione (TDZD), (2′Z,3′E)-6-bromoindirubin-3′-oxime (BIO), its negative control compound 1-methyl-BIO (ME-BIO), the Src inhibitor 4-amino-5-(4-chlorophenyl)-7-( t -butyl)pyrazolo[3,4-d]pyrimidine (PP2), and its inactive control 4-amino-7-phenylpyrazol [3,4-d]pyrimidine (PP3), the JNK inhibitor 1,3-benzothiazol-2-yl-(2-((2-(3 pyridinyl)ethyl)amino)-4-pyrimidinyl)acetonitrile (AS601645), and antibodies to actin were from EMD Bioscience (San Diego, CA).

    Solubility:

    Article Title: Increased operant responding for ethanol in male C57BL/6J mice: specific regulation by the ERK1/2, but not JNK, MAP kinase pathway
    Article Snippet: Because of solubility concerns at the higher concentrations of SL 327, the injection volume for this dose–effect curve was 2 ml/100 g BW. .. The c-Jun N-terminal kinase (JNK) inhibitor, AS 601245 (1,3-benzothiazol-2-yl-(2-((2-(3-pyridinyl)ethyl)amino)-4-pyrimidinyl)acetonitrile; EMD Biosciences, San Diego, CA, USA) was freshly suspended in 0.5% carboxymethylcellulose (CMC; Sigma-Aldrich, St. Louis, MO, USA), 0.25% Tween 20 (Sigma-Aldrich), and dH2 O.

    Concentration Assay:

    Article Title: The contribution of c-Jun N-terminal kinase activation and subsequent Bcl-2 phosphorylation to apoptosis induction in human B-cells is dependent on the mode of action of specific stresses
    Article Snippet: The JNK inhibitor SP600125 (SP6; Anthra[1,9- cd ]pyrazol-6(2 H )-one-1,9-pyrazoloanthron), JNK inhibitor II (a structural but inactive analogue of SP6 serving as a negative control; N1-methyl-1,9,pyrazoloanthrone), JNK inhibitor V, (a structurally unrelated JNK inhibitor, 1,3,-benzothiazole-2-yl-(2-(2-(3-pyridinyl)ethyl)amino)-4-pyrimidinyl acetonitrile), the ERK inhibitor U0126, and p38 inhibitor SB202190 (SB2), were obtained from Calbiochem Inc. All inhibitors were dissolved in DMSO. .. Thus, concentrations of inhibitors in their range of 10 – 20 µM were necessary to block the activities of the respective kinases in the absence of measurable, non-specific, effects on other kinases.

    Small Interfering RNA:

    Article Title: cAMP-guanine exchange factor protection from bile acid-induced hepatocyte apoptosis involves glycogen synthase kinase regulation of c-Jun NH2-terminal kinase
    Article Snippet: Additional GSK inhibitors 4-benzyl-2-methyl-1,2,4-thiadiazolidine-3,5-dione (TDZD), (2′Z,3′E)-6-bromoindirubin-3′-oxime (BIO), its negative control compound 1-methyl-BIO (ME-BIO), the Src inhibitor 4-amino-5-(4-chlorophenyl)-7-( t -butyl)pyrazolo[3,4-d]pyrimidine (PP2), and its inactive control 4-amino-7-phenylpyrazol [3,4-d]pyrimidine (PP3), the JNK inhibitor 1,3-benzothiazol-2-yl-(2-((2-(3 pyridinyl)ethyl)amino)-4-pyrimidinyl)acetonitrile (AS601645), and antibodies to actin were from EMD Bioscience (San Diego, CA). .. Additional GSK inhibitors 4-benzyl-2-methyl-1,2,4-thiadiazolidine-3,5-dione (TDZD), (2′Z,3′E)-6-bromoindirubin-3′-oxime (BIO), its negative control compound 1-methyl-BIO (ME-BIO), the Src inhibitor 4-amino-5-(4-chlorophenyl)-7-( t -butyl)pyrazolo[3,4-d]pyrimidine (PP2), and its inactive control 4-amino-7-phenylpyrazol [3,4-d]pyrimidine (PP3), the JNK inhibitor 1,3-benzothiazol-2-yl-(2-((2-(3 pyridinyl)ethyl)amino)-4-pyrimidinyl)acetonitrile (AS601645), and antibodies to actin were from EMD Bioscience (San Diego, CA).

    Blocking Assay:

    Article Title: The contribution of c-Jun N-terminal kinase activation and subsequent Bcl-2 phosphorylation to apoptosis induction in human B-cells is dependent on the mode of action of specific stresses
    Article Snippet: The JNK inhibitor SP600125 (SP6; Anthra[1,9- cd ]pyrazol-6(2 H )-one-1,9-pyrazoloanthron), JNK inhibitor II (a structural but inactive analogue of SP6 serving as a negative control; N1-methyl-1,9,pyrazoloanthrone), JNK inhibitor V, (a structurally unrelated JNK inhibitor, 1,3,-benzothiazole-2-yl-(2-(2-(3-pyridinyl)ethyl)amino)-4-pyrimidinyl acetonitrile), the ERK inhibitor U0126, and p38 inhibitor SB202190 (SB2), were obtained from Calbiochem Inc. All inhibitors were dissolved in DMSO. .. The JNK inhibitor SP600125 (SP6; Anthra[1,9- cd ]pyrazol-6(2 H )-one-1,9-pyrazoloanthron), JNK inhibitor II (a structural but inactive analogue of SP6 serving as a negative control; N1-methyl-1,9,pyrazoloanthrone), JNK inhibitor V, (a structurally unrelated JNK inhibitor, 1,3,-benzothiazole-2-yl-(2-(2-(3-pyridinyl)ethyl)amino)-4-pyrimidinyl acetonitrile), the ERK inhibitor U0126, and p38 inhibitor SB202190 (SB2), were obtained from Calbiochem Inc. All inhibitors were dissolved in DMSO.

    Injection:

    Article Title: Increased operant responding for ethanol in male C57BL/6J mice: specific regulation by the ERK1/2, but not JNK, MAP kinase pathway
    Article Snippet: Because of solubility concerns at the higher concentrations of SL 327, the injection volume for this dose–effect curve was 2 ml/100 g BW. .. The c-Jun N-terminal kinase (JNK) inhibitor, AS 601245 (1,3-benzothiazol-2-yl-(2-((2-(3-pyridinyl)ethyl)amino)-4-pyrimidinyl)acetonitrile; EMD Biosciences, San Diego, CA, USA) was freshly suspended in 0.5% carboxymethylcellulose (CMC; Sigma-Aldrich, St. Louis, MO, USA), 0.25% Tween 20 (Sigma-Aldrich), and dH2 O.

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  • 92
    Millipore jnk inhibitor v
    POH-induced apoptosis in U251 cells. U251 in the control condition (Control, A) . The cells were treated with 0.5 mM POH (POH, B) and 0.5 mM POH plus 0.5 μM <t>JNK</t> inhibitor V (POH + IJNK, C) . After 24 hours, the cells were immunostained for cleaved caspase-3 and the number of positive cells was analyzed (D) . Whereas POH induced cell apoptosis, the addition of the JNK inhibitor completely inhibited this effect. The addition of DMSO or JNK inhibitor V alone had no effect on cell death. Scale bar: 20 μm.*P
    Jnk Inhibitor V, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jnk inhibitor v/product/Millipore
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    jnk inhibitor v - by Bioz Stars, 2020-01
    92/100 stars
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    94
    Millipore mobile phase b
    POH-induced apoptosis in U251 cells. U251 in the control condition (Control, A) . The cells were treated with 0.5 mM POH (POH, B) and 0.5 mM POH plus 0.5 μM <t>JNK</t> inhibitor V (POH + IJNK, C) . After 24 hours, the cells were immunostained for cleaved caspase-3 and the number of positive cells was analyzed (D) . Whereas POH induced cell apoptosis, the addition of the JNK inhibitor completely inhibited this effect. The addition of DMSO or JNK inhibitor V alone had no effect on cell death. Scale bar: 20 μm.*P
    Mobile Phase B, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mobile phase b/product/Millipore
    Average 94 stars, based on 10 article reviews
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    78
    Millipore trypsin digestion buffer
    POH-induced apoptosis in U251 cells. U251 in the control condition (Control, A) . The cells were treated with 0.5 mM POH (POH, B) and 0.5 mM POH plus 0.5 μM <t>JNK</t> inhibitor V (POH + IJNK, C) . After 24 hours, the cells were immunostained for cleaved caspase-3 and the number of positive cells was analyzed (D) . Whereas POH induced cell apoptosis, the addition of the JNK inhibitor completely inhibited this effect. The addition of DMSO or JNK inhibitor V alone had no effect on cell death. Scale bar: 20 μm.*P
    Trypsin Digestion Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypsin digestion buffer/product/Millipore
    Average 78 stars, based on 2 article reviews
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    76
    Millipore as 601245
    POH-induced apoptosis in U251 cells. U251 in the control condition (Control, A) . The cells were treated with 0.5 mM POH (POH, B) and 0.5 mM POH plus 0.5 μM <t>JNK</t> inhibitor V (POH + IJNK, C) . After 24 hours, the cells were immunostained for cleaved caspase-3 and the number of positive cells was analyzed (D) . Whereas POH induced cell apoptosis, the addition of the JNK inhibitor completely inhibited this effect. The addition of DMSO or JNK inhibitor V alone had no effect on cell death. Scale bar: 20 μm.*P
    As 601245, supplied by Millipore, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/as 601245/product/Millipore
    Average 76 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    as 601245 - by Bioz Stars, 2020-01
    76/100 stars
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    Image Search Results


    POH-induced apoptosis in U251 cells. U251 in the control condition (Control, A) . The cells were treated with 0.5 mM POH (POH, B) and 0.5 mM POH plus 0.5 μM JNK inhibitor V (POH + IJNK, C) . After 24 hours, the cells were immunostained for cleaved caspase-3 and the number of positive cells was analyzed (D) . Whereas POH induced cell apoptosis, the addition of the JNK inhibitor completely inhibited this effect. The addition of DMSO or JNK inhibitor V alone had no effect on cell death. Scale bar: 20 μm.*P

    Journal: Molecular Cancer

    Article Title: Na/K-ATPase as a target for anticancer drugs: studies with perillyl alcohol

    doi: 10.1186/s12943-015-0374-5

    Figure Lengend Snippet: POH-induced apoptosis in U251 cells. U251 in the control condition (Control, A) . The cells were treated with 0.5 mM POH (POH, B) and 0.5 mM POH plus 0.5 μM JNK inhibitor V (POH + IJNK, C) . After 24 hours, the cells were immunostained for cleaved caspase-3 and the number of positive cells was analyzed (D) . Whereas POH induced cell apoptosis, the addition of the JNK inhibitor completely inhibited this effect. The addition of DMSO or JNK inhibitor V alone had no effect on cell death. Scale bar: 20 μm.*P

    Article Snippet: U87 and U251 cells were pretreated for 30 minutes with JNK inhibitor V [1,3-Benzothiazol-2-yl-(2-((2-(3-pyridinyl)ethyl)amino)-4-pyrimidinyl)acetonitrile; Calbiochem], an inhibitor of JNK1/2 activation, before treatment with 0.5 mM POH and 0.5 mM POH plus 0.5 μM JNK inhibitor V. After 24 hours of incubation, the cells were suspended in annexin and propidium iodide binding buffer as specified in the TACS Annexin V-FITC apoptosis detection kit (R & D Systems).

    Techniques:

    The effects of JNK inhibition on the induction of cell death by POH in U251 cells. Before treatment, U251 cells were incubated without (A and B) or with (C and D) JNK inhibitor V (0.5 μM) for 30 minutes. The cells were treated with 0.1% DMSO (A) , 0.5 mM POH (B) 0.1% DMSO plus JNK inhibitor V (C) , and 0.5 mM POH plus JNK inhibitor V (D) . After 24 hours of incubation, the cells were stained with annexin V-FITC and propidium iodide and analyzed by flow cytometry. Figure 7E represents the percentage of dead cells as indicated by early apoptosis and late apoptosis or necrosis (right lower quadrant + right upper quadrant, respectively), which was calculated from the data shown in Figures 7A-D. The data were expressed as the means ± SD from at least three different experiments. ***p

    Journal: Molecular Cancer

    Article Title: Na/K-ATPase as a target for anticancer drugs: studies with perillyl alcohol

    doi: 10.1186/s12943-015-0374-5

    Figure Lengend Snippet: The effects of JNK inhibition on the induction of cell death by POH in U251 cells. Before treatment, U251 cells were incubated without (A and B) or with (C and D) JNK inhibitor V (0.5 μM) for 30 minutes. The cells were treated with 0.1% DMSO (A) , 0.5 mM POH (B) 0.1% DMSO plus JNK inhibitor V (C) , and 0.5 mM POH plus JNK inhibitor V (D) . After 24 hours of incubation, the cells were stained with annexin V-FITC and propidium iodide and analyzed by flow cytometry. Figure 7E represents the percentage of dead cells as indicated by early apoptosis and late apoptosis or necrosis (right lower quadrant + right upper quadrant, respectively), which was calculated from the data shown in Figures 7A-D. The data were expressed as the means ± SD from at least three different experiments. ***p

    Article Snippet: U87 and U251 cells were pretreated for 30 minutes with JNK inhibitor V [1,3-Benzothiazol-2-yl-(2-((2-(3-pyridinyl)ethyl)amino)-4-pyrimidinyl)acetonitrile; Calbiochem], an inhibitor of JNK1/2 activation, before treatment with 0.5 mM POH and 0.5 mM POH plus 0.5 μM JNK inhibitor V. After 24 hours of incubation, the cells were suspended in annexin and propidium iodide binding buffer as specified in the TACS Annexin V-FITC apoptosis detection kit (R & D Systems).

    Techniques: Inhibition, Incubation, Staining, Flow Cytometry, Cytometry

    POH-induced apoptosis in U87 cells. U87 in the control condition (Control, A) . The cells were treated with 0.5 mM POH (POH, B) and 0.5 mM POH plus 0.5 μM JNK inhibitor V (POH + IJNK, C) . After 24 hours, the cells were immunostained for cleaved caspase-3 and the number of positive cells was analyzed (D) . Whereas POH induced cell apoptosis, the addition of the JNK inhibitor completely inhibited this effect. The addition of DMSO or JNK inhibitor V alone had no effect on cell death. Scale bar: 20 μm.*P

    Journal: Molecular Cancer

    Article Title: Na/K-ATPase as a target for anticancer drugs: studies with perillyl alcohol

    doi: 10.1186/s12943-015-0374-5

    Figure Lengend Snippet: POH-induced apoptosis in U87 cells. U87 in the control condition (Control, A) . The cells were treated with 0.5 mM POH (POH, B) and 0.5 mM POH plus 0.5 μM JNK inhibitor V (POH + IJNK, C) . After 24 hours, the cells were immunostained for cleaved caspase-3 and the number of positive cells was analyzed (D) . Whereas POH induced cell apoptosis, the addition of the JNK inhibitor completely inhibited this effect. The addition of DMSO or JNK inhibitor V alone had no effect on cell death. Scale bar: 20 μm.*P

    Article Snippet: U87 and U251 cells were pretreated for 30 minutes with JNK inhibitor V [1,3-Benzothiazol-2-yl-(2-((2-(3-pyridinyl)ethyl)amino)-4-pyrimidinyl)acetonitrile; Calbiochem], an inhibitor of JNK1/2 activation, before treatment with 0.5 mM POH and 0.5 mM POH plus 0.5 μM JNK inhibitor V. After 24 hours of incubation, the cells were suspended in annexin and propidium iodide binding buffer as specified in the TACS Annexin V-FITC apoptosis detection kit (R & D Systems).

    Techniques:

    The effects of JNK inhibition on the induction of cell death by POH in U87 cells. Before treatment, U87 cells were incubated without (A and B) or with (C and D) JNK inhibitor V (0.5 μM) for 30 minutes. The cells were treated with 0.1% DMSO (A), 0.5 mM POH (B) 0.1% DMSO plus JNK inhibitor V (C) , and 0.5 mM POH plus JNK inhibitor V (D) . After 24 hours of incubation, the cells were stained with annexin V-FITC and propidium iodide and analyzed by flow cytometry. Figure 6E represents the percentage of dead cells indicated by early apoptosis and late apoptosis or necrosis (right lower quadrant + right upper quadrant, respectively), which was calculated from the data shown in Figures 6A-D. The data were expressed as the means ± SD from at least three different experiments. ***p

    Journal: Molecular Cancer

    Article Title: Na/K-ATPase as a target for anticancer drugs: studies with perillyl alcohol

    doi: 10.1186/s12943-015-0374-5

    Figure Lengend Snippet: The effects of JNK inhibition on the induction of cell death by POH in U87 cells. Before treatment, U87 cells were incubated without (A and B) or with (C and D) JNK inhibitor V (0.5 μM) for 30 minutes. The cells were treated with 0.1% DMSO (A), 0.5 mM POH (B) 0.1% DMSO plus JNK inhibitor V (C) , and 0.5 mM POH plus JNK inhibitor V (D) . After 24 hours of incubation, the cells were stained with annexin V-FITC and propidium iodide and analyzed by flow cytometry. Figure 6E represents the percentage of dead cells indicated by early apoptosis and late apoptosis or necrosis (right lower quadrant + right upper quadrant, respectively), which was calculated from the data shown in Figures 6A-D. The data were expressed as the means ± SD from at least three different experiments. ***p

    Article Snippet: U87 and U251 cells were pretreated for 30 minutes with JNK inhibitor V [1,3-Benzothiazol-2-yl-(2-((2-(3-pyridinyl)ethyl)amino)-4-pyrimidinyl)acetonitrile; Calbiochem], an inhibitor of JNK1/2 activation, before treatment with 0.5 mM POH and 0.5 mM POH plus 0.5 μM JNK inhibitor V. After 24 hours of incubation, the cells were suspended in annexin and propidium iodide binding buffer as specified in the TACS Annexin V-FITC apoptosis detection kit (R & D Systems).

    Techniques: Inhibition, Incubation, Staining, Flow Cytometry, Cytometry