ace2 oti1g4  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc ace2 oti1g4
    Expression of SARS-CoV-2 entry factors in BRON and ALV organoids. (A) <t>ACE2</t> (left) and TMPRSS2 (right) expression is detected in patient L2 organoids of bronchiolar (BRON) and alveolar (ALV) differentiation from passage 1-4. Relative quantification was performed using the Livac method and alveolar organoids are normalized to the average delta Ct of bronchiolar organoids. The mean value of three technical replicates for each passage are shown as individual data points. GraphPadPrism v9 was used to calculate and display the geometric mean and 90% confidence interval of the biological replicates (passages). Statistics were performed on the delta Ct values using the Mann-Whitney test. No statistically significant differences were observed in the median deltaCt values of ACE2 between BRON and ALV cultures. (B) Immunofluorescent staining of formalin fixed paraffin embedded (FFPE) sections of bronchiolar (BRON) organoids (representative images from patient L2 passage 2), display co-staining of the cilia marker (ARL13B) and the SARS-CoV-2 receptor ACE2 (transparent arrowheads). ACE2 staining alone (white arrowheads). Scale bar equals 100 µm. (C) Immunofluorescent staining of formalin fixed paraffin embedded (FFPE) sections of alveolar (ALV) organoids (representative images from patient L2 passage 2), display co-staining of the cilia marker (ARL13B) and the SARS-CoV-2 receptor ACE2 (transparent arrowheads). Scale bar equals 100 µm. ns, not significant.
    Ace2 Oti1g4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ace2 oti1g4/product/Cell Signaling Technology Inc
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ace2 oti1g4 - by Bioz Stars, 2023-03
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    1) Product Images from "Human Organotypic Airway and Lung Organoid Cells of Bronchiolar and Alveolar Differentiation Are Permissive to Infection by Influenza and SARS-CoV-2 Respiratory Virus"

    Article Title: Human Organotypic Airway and Lung Organoid Cells of Bronchiolar and Alveolar Differentiation Are Permissive to Infection by Influenza and SARS-CoV-2 Respiratory Virus

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2022.841447

    Expression of SARS-CoV-2 entry factors in BRON and ALV organoids. (A) ACE2 (left) and TMPRSS2 (right) expression is detected in patient L2 organoids of bronchiolar (BRON) and alveolar (ALV) differentiation from passage 1-4. Relative quantification was performed using the Livac method and alveolar organoids are normalized to the average delta Ct of bronchiolar organoids. The mean value of three technical replicates for each passage are shown as individual data points. GraphPadPrism v9 was used to calculate and display the geometric mean and 90% confidence interval of the biological replicates (passages). Statistics were performed on the delta Ct values using the Mann-Whitney test. No statistically significant differences were observed in the median deltaCt values of ACE2 between BRON and ALV cultures. (B) Immunofluorescent staining of formalin fixed paraffin embedded (FFPE) sections of bronchiolar (BRON) organoids (representative images from patient L2 passage 2), display co-staining of the cilia marker (ARL13B) and the SARS-CoV-2 receptor ACE2 (transparent arrowheads). ACE2 staining alone (white arrowheads). Scale bar equals 100 µm. (C) Immunofluorescent staining of formalin fixed paraffin embedded (FFPE) sections of alveolar (ALV) organoids (representative images from patient L2 passage 2), display co-staining of the cilia marker (ARL13B) and the SARS-CoV-2 receptor ACE2 (transparent arrowheads). Scale bar equals 100 µm. ns, not significant.
    Figure Legend Snippet: Expression of SARS-CoV-2 entry factors in BRON and ALV organoids. (A) ACE2 (left) and TMPRSS2 (right) expression is detected in patient L2 organoids of bronchiolar (BRON) and alveolar (ALV) differentiation from passage 1-4. Relative quantification was performed using the Livac method and alveolar organoids are normalized to the average delta Ct of bronchiolar organoids. The mean value of three technical replicates for each passage are shown as individual data points. GraphPadPrism v9 was used to calculate and display the geometric mean and 90% confidence interval of the biological replicates (passages). Statistics were performed on the delta Ct values using the Mann-Whitney test. No statistically significant differences were observed in the median deltaCt values of ACE2 between BRON and ALV cultures. (B) Immunofluorescent staining of formalin fixed paraffin embedded (FFPE) sections of bronchiolar (BRON) organoids (representative images from patient L2 passage 2), display co-staining of the cilia marker (ARL13B) and the SARS-CoV-2 receptor ACE2 (transparent arrowheads). ACE2 staining alone (white arrowheads). Scale bar equals 100 µm. (C) Immunofluorescent staining of formalin fixed paraffin embedded (FFPE) sections of alveolar (ALV) organoids (representative images from patient L2 passage 2), display co-staining of the cilia marker (ARL13B) and the SARS-CoV-2 receptor ACE2 (transparent arrowheads). Scale bar equals 100 µm. ns, not significant.

    Techniques Used: Expressing, MANN-WHITNEY, Staining, Formalin-fixed Paraffin-Embedded, Marker

    Product information for TaqMan probes used in RT-qPCR assays.
    Figure Legend Snippet: Product information for TaqMan probes used in RT-qPCR assays.

    Techniques Used: Gene Assay

    The product information and dilution of primary used to detect the various target epitopes in this study.
    Figure Legend Snippet: The product information and dilution of primary used to detect the various target epitopes in this study.

    Techniques Used: Luciferase

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    Cell Signaling Technology Inc ace2 oti1g4
    Expression of SARS-CoV-2 entry factors in BRON and ALV organoids. (A) <t>ACE2</t> (left) and TMPRSS2 (right) expression is detected in patient L2 organoids of bronchiolar (BRON) and alveolar (ALV) differentiation from passage 1-4. Relative quantification was performed using the Livac method and alveolar organoids are normalized to the average delta Ct of bronchiolar organoids. The mean value of three technical replicates for each passage are shown as individual data points. GraphPadPrism v9 was used to calculate and display the geometric mean and 90% confidence interval of the biological replicates (passages). Statistics were performed on the delta Ct values using the Mann-Whitney test. No statistically significant differences were observed in the median deltaCt values of ACE2 between BRON and ALV cultures. (B) Immunofluorescent staining of formalin fixed paraffin embedded (FFPE) sections of bronchiolar (BRON) organoids (representative images from patient L2 passage 2), display co-staining of the cilia marker (ARL13B) and the SARS-CoV-2 receptor ACE2 (transparent arrowheads). ACE2 staining alone (white arrowheads). Scale bar equals 100 µm. (C) Immunofluorescent staining of formalin fixed paraffin embedded (FFPE) sections of alveolar (ALV) organoids (representative images from patient L2 passage 2), display co-staining of the cilia marker (ARL13B) and the SARS-CoV-2 receptor ACE2 (transparent arrowheads). Scale bar equals 100 µm. ns, not significant.
    Ace2 Oti1g4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ace2 oti1g4/product/Cell Signaling Technology Inc
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ace2 oti1g4 - by Bioz Stars, 2023-03
    93/100 stars
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    Expression of SARS-CoV-2 entry factors in BRON and ALV organoids. (A) ACE2 (left) and TMPRSS2 (right) expression is detected in patient L2 organoids of bronchiolar (BRON) and alveolar (ALV) differentiation from passage 1-4. Relative quantification was performed using the Livac method and alveolar organoids are normalized to the average delta Ct of bronchiolar organoids. The mean value of three technical replicates for each passage are shown as individual data points. GraphPadPrism v9 was used to calculate and display the geometric mean and 90% confidence interval of the biological replicates (passages). Statistics were performed on the delta Ct values using the Mann-Whitney test. No statistically significant differences were observed in the median deltaCt values of ACE2 between BRON and ALV cultures. (B) Immunofluorescent staining of formalin fixed paraffin embedded (FFPE) sections of bronchiolar (BRON) organoids (representative images from patient L2 passage 2), display co-staining of the cilia marker (ARL13B) and the SARS-CoV-2 receptor ACE2 (transparent arrowheads). ACE2 staining alone (white arrowheads). Scale bar equals 100 µm. (C) Immunofluorescent staining of formalin fixed paraffin embedded (FFPE) sections of alveolar (ALV) organoids (representative images from patient L2 passage 2), display co-staining of the cilia marker (ARL13B) and the SARS-CoV-2 receptor ACE2 (transparent arrowheads). Scale bar equals 100 µm. ns, not significant.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Human Organotypic Airway and Lung Organoid Cells of Bronchiolar and Alveolar Differentiation Are Permissive to Infection by Influenza and SARS-CoV-2 Respiratory Virus

    doi: 10.3389/fcimb.2022.841447

    Figure Lengend Snippet: Expression of SARS-CoV-2 entry factors in BRON and ALV organoids. (A) ACE2 (left) and TMPRSS2 (right) expression is detected in patient L2 organoids of bronchiolar (BRON) and alveolar (ALV) differentiation from passage 1-4. Relative quantification was performed using the Livac method and alveolar organoids are normalized to the average delta Ct of bronchiolar organoids. The mean value of three technical replicates for each passage are shown as individual data points. GraphPadPrism v9 was used to calculate and display the geometric mean and 90% confidence interval of the biological replicates (passages). Statistics were performed on the delta Ct values using the Mann-Whitney test. No statistically significant differences were observed in the median deltaCt values of ACE2 between BRON and ALV cultures. (B) Immunofluorescent staining of formalin fixed paraffin embedded (FFPE) sections of bronchiolar (BRON) organoids (representative images from patient L2 passage 2), display co-staining of the cilia marker (ARL13B) and the SARS-CoV-2 receptor ACE2 (transparent arrowheads). ACE2 staining alone (white arrowheads). Scale bar equals 100 µm. (C) Immunofluorescent staining of formalin fixed paraffin embedded (FFPE) sections of alveolar (ALV) organoids (representative images from patient L2 passage 2), display co-staining of the cilia marker (ARL13B) and the SARS-CoV-2 receptor ACE2 (transparent arrowheads). Scale bar equals 100 µm. ns, not significant.

    Article Snippet: ACE2 , ACE2 (OTI1G4) #74512 , 1:200 , Cell Signaling.

    Techniques: Expressing, MANN-WHITNEY, Staining, Formalin-fixed Paraffin-Embedded, Marker

    Product information for TaqMan probes used in RT-qPCR assays.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Human Organotypic Airway and Lung Organoid Cells of Bronchiolar and Alveolar Differentiation Are Permissive to Infection by Influenza and SARS-CoV-2 Respiratory Virus

    doi: 10.3389/fcimb.2022.841447

    Figure Lengend Snippet: Product information for TaqMan probes used in RT-qPCR assays.

    Article Snippet: ACE2 , ACE2 (OTI1G4) #74512 , 1:200 , Cell Signaling.

    Techniques: Gene Assay

    The product information and dilution of primary used to detect the various target epitopes in this study.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Human Organotypic Airway and Lung Organoid Cells of Bronchiolar and Alveolar Differentiation Are Permissive to Infection by Influenza and SARS-CoV-2 Respiratory Virus

    doi: 10.3389/fcimb.2022.841447

    Figure Lengend Snippet: The product information and dilution of primary used to detect the various target epitopes in this study.

    Article Snippet: ACE2 , ACE2 (OTI1G4) #74512 , 1:200 , Cell Signaling.

    Techniques: Luciferase