accella hplc pump system  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Thermo Fisher accella hplc pump system
    Accella Hplc Pump System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accella hplc pump system/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    accella hplc pump system - by Bioz Stars, 2020-07
    86/100 stars

    Related Products / Commonly Used Together

    mass spectrometer

    Images

    Related Articles

    High Performance Liquid Chromatography:

    Article Title:
    Article Snippet: .. The mass spectrometer was coupled to an Accella HPLC pump system (Thermo Fisher Scientific) and a CTC PAL autosampler. .. Analytes were separated by gradient elution employing two Acquity BEH C18 columns (2.1 × 50 mm, 1.7 µ ; Waters Corp., Milford, MA) that were heated at 50°C.

    Mass Spectrometry:

    Article Title:
    Article Snippet: .. The mass spectrometer was coupled to an Accella HPLC pump system (Thermo Fisher Scientific) and a CTC PAL autosampler. .. Analytes were separated by gradient elution employing two Acquity BEH C18 columns (2.1 × 50 mm, 1.7 µ ; Waters Corp., Milford, MA) that were heated at 50°C.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher hplc system
    Functional characterization of the PKS19 cluster, a putative polyketide biosynthetic gene cluster that is unique to F. fujikuroi . (A) Position and organization of the PKS19 gene cluster on F. fujikuroi chromosome VIII, GC content, distribution of active histone marks, and gene expression in the PKS19 cluster. Histone marks are described in the legend for Figure 3 . Expression data are from microarray analysis of wild-type F. fujikuroi (strain IMI58289). Values are log 2 change in expression in a high versus low-nitrogen medium. H3K9ac and gene expression are correlated, as both are increased in the high-nitrogen medium. Some genes exhibited increased levels of H3K4me2 in the high-nitrogen medium, which also suggests transcription. (B) Chemical analysis of the SM product(s) of the PKS19 cluster. The traces show the combined extracted ion chromatograms for metabolites with molecular formulas [C 12 H 16 O 4 +H] + , [C 12 H 18 O 5 +H] + and [C 12 H 18 O 4 +H] + determined by <t>HPLC-FTMS</t> of culture fluids from F. fujikuroi strains: IMI58289, wild-type strain; OE::TF, a strain over-expressing the transcription factor encoded by FFUJ_12242; OE::PKS19, a strain over-expressing the PKS19 gene FFUJ_12239; and OE::TF/OE::PKS19, a strain over-expressing both FFUJ-12242 and FFUJ_12239. (C) Northern blot analysis of PKS19 cluster genes in strains IMI58289 (WT), OE::TF, OE::PKS19 and OE::TF/OE::PKS19. (D) UV spectra of metabolites corresponding to peaks 1 through 4 from chromatograms shown in C. The similar spectra of the metabolites suggest structural similarity.
    Hplc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 587 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc system/product/Thermo Fisher
    Average 99 stars, based on 587 article reviews
    Price from $9.99 to $1999.99
    hplc system - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    88
    Thermo Fisher accela quaternary hplc pump
    Functional characterization of the PKS19 cluster, a putative polyketide biosynthetic gene cluster that is unique to F. fujikuroi . (A) Position and organization of the PKS19 gene cluster on F. fujikuroi chromosome VIII, GC content, distribution of active histone marks, and gene expression in the PKS19 cluster. Histone marks are described in the legend for Figure 3 . Expression data are from microarray analysis of wild-type F. fujikuroi (strain IMI58289). Values are log 2 change in expression in a high versus low-nitrogen medium. H3K9ac and gene expression are correlated, as both are increased in the high-nitrogen medium. Some genes exhibited increased levels of H3K4me2 in the high-nitrogen medium, which also suggests transcription. (B) Chemical analysis of the SM product(s) of the PKS19 cluster. The traces show the combined extracted ion chromatograms for metabolites with molecular formulas [C 12 H 16 O 4 +H] + , [C 12 H 18 O 5 +H] + and [C 12 H 18 O 4 +H] + determined by <t>HPLC-FTMS</t> of culture fluids from F. fujikuroi strains: IMI58289, wild-type strain; OE::TF, a strain over-expressing the transcription factor encoded by FFUJ_12242; OE::PKS19, a strain over-expressing the PKS19 gene FFUJ_12239; and OE::TF/OE::PKS19, a strain over-expressing both FFUJ-12242 and FFUJ_12239. (C) Northern blot analysis of PKS19 cluster genes in strains IMI58289 (WT), OE::TF, OE::PKS19 and OE::TF/OE::PKS19. (D) UV spectra of metabolites corresponding to peaks 1 through 4 from chromatograms shown in C. The similar spectra of the metabolites suggest structural similarity.
    Accela Quaternary Hplc Pump, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accela quaternary hplc pump/product/Thermo Fisher
    Average 88 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    accela quaternary hplc pump - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    90
    Thermo Fisher high pressure quaternary pump accela 1250
    Functional characterization of the PKS19 cluster, a putative polyketide biosynthetic gene cluster that is unique to F. fujikuroi . (A) Position and organization of the PKS19 gene cluster on F. fujikuroi chromosome VIII, GC content, distribution of active histone marks, and gene expression in the PKS19 cluster. Histone marks are described in the legend for Figure 3 . Expression data are from microarray analysis of wild-type F. fujikuroi (strain IMI58289). Values are log 2 change in expression in a high versus low-nitrogen medium. H3K9ac and gene expression are correlated, as both are increased in the high-nitrogen medium. Some genes exhibited increased levels of H3K4me2 in the high-nitrogen medium, which also suggests transcription. (B) Chemical analysis of the SM product(s) of the PKS19 cluster. The traces show the combined extracted ion chromatograms for metabolites with molecular formulas [C 12 H 16 O 4 +H] + , [C 12 H 18 O 5 +H] + and [C 12 H 18 O 4 +H] + determined by <t>HPLC-FTMS</t> of culture fluids from F. fujikuroi strains: IMI58289, wild-type strain; OE::TF, a strain over-expressing the transcription factor encoded by FFUJ_12242; OE::PKS19, a strain over-expressing the PKS19 gene FFUJ_12239; and OE::TF/OE::PKS19, a strain over-expressing both FFUJ-12242 and FFUJ_12239. (C) Northern blot analysis of PKS19 cluster genes in strains IMI58289 (WT), OE::TF, OE::PKS19 and OE::TF/OE::PKS19. (D) UV spectra of metabolites corresponding to peaks 1 through 4 from chromatograms shown in C. The similar spectra of the metabolites suggest structural similarity.
    High Pressure Quaternary Pump Accela 1250, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high pressure quaternary pump accela 1250/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high pressure quaternary pump accela 1250 - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    Image Search Results


    Functional characterization of the PKS19 cluster, a putative polyketide biosynthetic gene cluster that is unique to F. fujikuroi . (A) Position and organization of the PKS19 gene cluster on F. fujikuroi chromosome VIII, GC content, distribution of active histone marks, and gene expression in the PKS19 cluster. Histone marks are described in the legend for Figure 3 . Expression data are from microarray analysis of wild-type F. fujikuroi (strain IMI58289). Values are log 2 change in expression in a high versus low-nitrogen medium. H3K9ac and gene expression are correlated, as both are increased in the high-nitrogen medium. Some genes exhibited increased levels of H3K4me2 in the high-nitrogen medium, which also suggests transcription. (B) Chemical analysis of the SM product(s) of the PKS19 cluster. The traces show the combined extracted ion chromatograms for metabolites with molecular formulas [C 12 H 16 O 4 +H] + , [C 12 H 18 O 5 +H] + and [C 12 H 18 O 4 +H] + determined by HPLC-FTMS of culture fluids from F. fujikuroi strains: IMI58289, wild-type strain; OE::TF, a strain over-expressing the transcription factor encoded by FFUJ_12242; OE::PKS19, a strain over-expressing the PKS19 gene FFUJ_12239; and OE::TF/OE::PKS19, a strain over-expressing both FFUJ-12242 and FFUJ_12239. (C) Northern blot analysis of PKS19 cluster genes in strains IMI58289 (WT), OE::TF, OE::PKS19 and OE::TF/OE::PKS19. (D) UV spectra of metabolites corresponding to peaks 1 through 4 from chromatograms shown in C. The similar spectra of the metabolites suggest structural similarity.

    Journal: PLoS Pathogens

    Article Title: Deciphering the Cryptic Genome: Genome-wide Analyses of the Rice Pathogen Fusarium fujikuroi Reveal Complex Regulation of Secondary Metabolism and Novel Metabolites

    doi: 10.1371/journal.ppat.1003475

    Figure Lengend Snippet: Functional characterization of the PKS19 cluster, a putative polyketide biosynthetic gene cluster that is unique to F. fujikuroi . (A) Position and organization of the PKS19 gene cluster on F. fujikuroi chromosome VIII, GC content, distribution of active histone marks, and gene expression in the PKS19 cluster. Histone marks are described in the legend for Figure 3 . Expression data are from microarray analysis of wild-type F. fujikuroi (strain IMI58289). Values are log 2 change in expression in a high versus low-nitrogen medium. H3K9ac and gene expression are correlated, as both are increased in the high-nitrogen medium. Some genes exhibited increased levels of H3K4me2 in the high-nitrogen medium, which also suggests transcription. (B) Chemical analysis of the SM product(s) of the PKS19 cluster. The traces show the combined extracted ion chromatograms for metabolites with molecular formulas [C 12 H 16 O 4 +H] + , [C 12 H 18 O 5 +H] + and [C 12 H 18 O 4 +H] + determined by HPLC-FTMS of culture fluids from F. fujikuroi strains: IMI58289, wild-type strain; OE::TF, a strain over-expressing the transcription factor encoded by FFUJ_12242; OE::PKS19, a strain over-expressing the PKS19 gene FFUJ_12239; and OE::TF/OE::PKS19, a strain over-expressing both FFUJ-12242 and FFUJ_12239. (C) Northern blot analysis of PKS19 cluster genes in strains IMI58289 (WT), OE::TF, OE::PKS19 and OE::TF/OE::PKS19. (D) UV spectra of metabolites corresponding to peaks 1 through 4 from chromatograms shown in C. The similar spectra of the metabolites suggest structural similarity.

    Article Snippet: HPLC-FTMS The HPLC-FTMS system used an HPLC system (Accela LC with Accela Pump 60057-60010 and Accela Autosampler 60057-60020, Thermo Scientific, Dreieich, Germany) coupled to a Fourier transform mass spectrometer with a heated ESI source (LTQ Orbitrap XL, Thermo Scientific, Dreieich, Germany).

    Techniques: Functional Assay, Expressing, Microarray, High Performance Liquid Chromatography, Northern Blot

    Location of the NRPS/APS biosynthetic gene cluster on F. fujikuroi chromosome I, levels of histone modifications and gene expression within and flanking the cluster, and production of metabolites following overexpression of cluster genes APS2 and APS8 . A : Synteny between the apicidin gene cluster in F. semitectum [105] and the apicidin-like gene cluster in F. fujikuroi . B : Histone modifications and gene expression in and flanking the cluster. Histone marks are described in the legend to Figure 3 . Expression data were derived from microarray experiments in low and high nitrogen and are plotted as the changes in log 2 expression values in high-nitrogen medium compared to low-nitrogen medium. H3K9ac and gene expression are overall correlated, as both are increased under high nitrogen conditions. In some genes increased H3K4me2 was observed, also suggesting transcription. C : Chemical analysis of the product of the unique PKS19 gene cluster. The traces show the extracted ion chromatograms for [C 34 H 48 O 6 N 5 +H] + (first line) and [C 35 H 42 O 7 N 5 +H] + (second to fourth line) determined by HPLC-FTMS of an apicidin standard (first line) and of culture fluids from F. fujikuroi IMI58289, OE::APS8 and the OE::APS2/OE::APS8 mutant. D : UV spectra of apicidin and the apicidin-like compound. The similar spectra suggest a structural similarity.

    Journal: PLoS Pathogens

    Article Title: Deciphering the Cryptic Genome: Genome-wide Analyses of the Rice Pathogen Fusarium fujikuroi Reveal Complex Regulation of Secondary Metabolism and Novel Metabolites

    doi: 10.1371/journal.ppat.1003475

    Figure Lengend Snippet: Location of the NRPS/APS biosynthetic gene cluster on F. fujikuroi chromosome I, levels of histone modifications and gene expression within and flanking the cluster, and production of metabolites following overexpression of cluster genes APS2 and APS8 . A : Synteny between the apicidin gene cluster in F. semitectum [105] and the apicidin-like gene cluster in F. fujikuroi . B : Histone modifications and gene expression in and flanking the cluster. Histone marks are described in the legend to Figure 3 . Expression data were derived from microarray experiments in low and high nitrogen and are plotted as the changes in log 2 expression values in high-nitrogen medium compared to low-nitrogen medium. H3K9ac and gene expression are overall correlated, as both are increased under high nitrogen conditions. In some genes increased H3K4me2 was observed, also suggesting transcription. C : Chemical analysis of the product of the unique PKS19 gene cluster. The traces show the extracted ion chromatograms for [C 34 H 48 O 6 N 5 +H] + (first line) and [C 35 H 42 O 7 N 5 +H] + (second to fourth line) determined by HPLC-FTMS of an apicidin standard (first line) and of culture fluids from F. fujikuroi IMI58289, OE::APS8 and the OE::APS2/OE::APS8 mutant. D : UV spectra of apicidin and the apicidin-like compound. The similar spectra suggest a structural similarity.

    Article Snippet: HPLC-FTMS The HPLC-FTMS system used an HPLC system (Accela LC with Accela Pump 60057-60010 and Accela Autosampler 60057-60020, Thermo Scientific, Dreieich, Germany) coupled to a Fourier transform mass spectrometer with a heated ESI source (LTQ Orbitrap XL, Thermo Scientific, Dreieich, Germany).

    Techniques: Expressing, Over Expression, Derivative Assay, Microarray, High Performance Liquid Chromatography, Mutagenesis