Structured Review

Thermo Fisher accela
Accela, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/accela/product/Thermo Fisher
Average 90 stars, based on 39 article reviews
Price from $9.99 to $1999.99
accela - by Bioz Stars, 2020-07
90/100 stars

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High Performance Liquid Chromatography:

Article Title: Fluorophore Labeled Kinase Detects Ligands That Bind within the MAPK Insert of p38? Kinase
Article Snippet: .. High resolution electrospray ionization mass spectra (ESI-FTMS) were recorded on a Thermo LTQ Orbitrap (high resolution mass spectrometer from Thermo Electron) coupled to an ‘Accela’ HPLC System supplied with a ‘Hypersil GOLD’ column (Thermo Electron). .. Analytical TLC was carried out on Merck 60 F245 aluminum-backed silica gel plates.

Article Title: Rate-Determining and Rate-Limiting Steps in the Clearance and Excretion of a Potent and Selective p21-Activated Kinase Inhibitor: A Case Study of Rapid Hepatic Uptake and Slow Elimination in Rat
Article Snippet: .. Metabolite identification was carried out on an Accela (Thermo Fisher Scientific, San Jose, CA) HPLC coupled with an Orbitrap Velos (Thermo Fisher Scientific). .. Separation of metabolites was achieved using Acquity CSH C18 column (1.7 μm, 2.1 x 100 mm; Waters, Milford, MA) with a flow rate of 0.4 mL/min of mobile phases (A) 0.1% formic acid in water and (B) 0.1% formic acid in acetonitrile.

other:

Article Title: Cofactor Selectivity in Methylmalonyl Coenzyme A Mutase, a Model Cobamide-Dependent Enzyme
Article Snippet: Chemicals were obtained from the sources indicated: 5′-chloro-5′-deoxyadenosine, Santa Cruz Biotechnology; 7-methylbenzimidazole, Accela; 5-methyl-1H-benzimidazole, Acros Organics; phenol, J. T. Baker; zinc metal, Fisher Scientific; 5-methoxybenzimidazole, purine, and para -cresol, Alfa Aesar; methylmalonyl-CoA, methylmalonic acid, coenzyme A, adenosylcobalamin (coenzyme B12 ), cyanocobalamin, dicyanocobinamide, 6-methylpurine, 1H-imidazo[4,5-c]pyridine-4-amine (3-deazaadenine), benzimidazole, adenine hemisulfate, 5-azabenzimidazole, 1H-benzo[d]imidazol-7-amine (7-aminobenzimidazole), 2-methyl-1H-purine-6-amine (2-methyladenine), and bovine serum albumin (BSA), Sigma.

Mass Spectrometry:

Article Title: Fluorophore Labeled Kinase Detects Ligands That Bind within the MAPK Insert of p38? Kinase
Article Snippet: .. High resolution electrospray ionization mass spectra (ESI-FTMS) were recorded on a Thermo LTQ Orbitrap (high resolution mass spectrometer from Thermo Electron) coupled to an ‘Accela’ HPLC System supplied with a ‘Hypersil GOLD’ column (Thermo Electron). .. Analytical TLC was carried out on Merck 60 F245 aluminum-backed silica gel plates.

Article Title: Multifunctional Core-Shell Nanoparticles: Discovery of Previously Invisible Biomarkers
Article Snippet: .. The LC pump was an Accela (ThermoFisher Scientific) operated at 160 μL/min, and effluent was directed into the mass spectrometer using an IonMax source. ..

Article Title: Alteration of Striatal Tetrahydrobiopterin in Iron-Induced Unilateral Model of Parkinson's Disease
Article Snippet: .. The liquid chromatographic system was a Accela (Thermo Fisher Scientific Inc., Waltham, MA, USA) system equipped with a solvent delivery module, Nanospace SI-2 3133 (Shiseido Inc., Japan) with an autosampler, and connected to a Discovery Max (Thermo Fisher Scientific Inc.) quadrupole tandem mass spectrometer equipped with electrospray ionization. .. System control and data analyses were carried out with Xcalibur (Thermo Fisher Scientific Inc.).

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  • 89
    Thermo Fisher accell sirna
    Suppression of EGFP signal in M4A4 breast cancer cells transfected with <t>Accell</t> EGFP pool <t>siRNA</t> on the CBC-1 after 5 days in culture. Photomicrographs show merged bright field (BF) z-stacks and merged green fluorescence z-stacks (eGFP). EGFP Signal was expressed relative to controls ( n =5). a P
    Accell Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accell sirna/product/Thermo Fisher
    Average 89 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    accell sirna - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    85
    Thermo Fisher accell cxcr4 sirna
    Ubiquitin receptor binding is reduced after <t>CXCR4</t> gene silencing and CXCR4 ligands compete with ubiquitin for receptor-binding sites. A , CXCR4 was silenced with <t>siRNA</t> in THP1 cells followed by immunoblotting of whole cell lysates with anti-CXCR4 and anti-β-actin.
    Accell Cxcr4 Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accell cxcr4 sirna/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    accell cxcr4 sirna - by Bioz Stars, 2020-07
    85/100 stars
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    88
    Thermo Fisher accell nontargeting sirna
    NFAT5-knockdown attenuates osmolality-induced MCP-1 expression. Met5A cells were transfected with <t>siRNA</t> constructs for NFAT5 or with <t>nontargeting</t> siRNA as control as indicated. Cells were kept in isosmotic medium (gray column; 300 mosm/kg H 2 O) or were exposed to hyperosmotic medium (black column; 400 mosm/kg H 2 O). Medium osmolality was elevated by addition of glucose or NaCl as indicated, and cells were incubated for 24 h. (a) To demonstrate efficiency of NFAT5 knockdown, cells were processed for immunoblotting as described in Section 2 . To demonstrate comparable protein loading, the blots were also probed for actin. (b) For determination of MCP-1 secretion, medium samples were collected and the concentration of MCP-1 in the cell culture supernatant was determined by ELISA as described in Section 2 . Means ± SEM for n = 4 per point; * P
    Accell Nontargeting Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accell nontargeting sirna/product/Thermo Fisher
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    accell nontargeting sirna - by Bioz Stars, 2020-07
    88/100 stars
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    91
    Thermo Fisher accell human btn3a1 sirna
    ABCA1 interactions with <t>BTN3A1,</t> apoA-I and IPP. ( a ) ABCA1 and BTN3A1 co-immunoprecipitate in untreated and ZA-treated DC U937 and DC but not in CD14+ cells, and ZA treatment does not modify BTN3A1 expression. Pancadherin is employed as a control of equal protein loading (one out of three blots). ( b ) PLA of ABCA1-BTN3A1 interaction by confocal laser-scanning microscopy (ocular lens: × 10; objective: × 63). Ctrl: cells without primary antibodies. Scale bar, 10 μm; blue: nuclear staining (DAPI); green: ABCA1/BTN3A1 interaction (one out of the three experiments). ( c ) apoA-I is physically associated with ABCA1, not with BTN3A1. The expected molecular weight of apoA-I, ABCA1/apoA-I and BTN3A1/apoA-I are shown (one out of the three experiments). ( d ) ABCA1 and BTN3A1 expression in DC after incubation with <t>siRNA</t> for Abca1 (siABca1), Btn3a1 (siBtn3a1) or with scrambled non-targeting siRNA (scr). β-tubulin was employed as control of equal protein loading ( n =3). ( e ) IPP is physically associated with ABCA1 in untreated and ZA-treated DC. The two bands of 150 and 100 kDa in the dithiothreitol (DTT)- and trypsin-treated DC lane are detected with an antibody specific for the N-terminal extracellular loop of ABCA1 in immunoblotting (IB; left). Autoradiography signal of the IPP-ABCA1 interaction (right). Pancadherin is employed as a control of equal protein loading (one out of three experiments). ( f ) Extracellular IPP release in Abca1- and/or Btn3a 1-silenced DC left untreated (ZA-) or after ZA treatment (ZA+). The bars represent the mean±s.e.m. of three experiments (** P
    Accell Human Btn3a1 Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accell human btn3a1 sirna/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    accell human btn3a1 sirna - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    Image Search Results


    Suppression of EGFP signal in M4A4 breast cancer cells transfected with Accell EGFP pool siRNA on the CBC-1 after 5 days in culture. Photomicrographs show merged bright field (BF) z-stacks and merged green fluorescence z-stacks (eGFP). EGFP Signal was expressed relative to controls ( n =5). a P

    Journal: Hormones & cancer

    Article Title: The Cancer BioChip System: A Functional Genomic Assay for Anchorage-Independent Three-Dimensional Breast Cancer Cell Growth

    doi: 10.1007/s12672-012-0116-8

    Figure Lengend Snippet: Suppression of EGFP signal in M4A4 breast cancer cells transfected with Accell EGFP pool siRNA on the CBC-1 after 5 days in culture. Photomicrographs show merged bright field (BF) z-stacks and merged green fluorescence z-stacks (eGFP). EGFP Signal was expressed relative to controls ( n =5). a P

    Article Snippet: Accell siRNA (Thermo Fisher Dharmacon, Lafayette, CO) or Mission TRC shRNA-expressing lentiviral vectors (Sigma, Saint Louis, MO) were employed in this study.

    Techniques: Transfection, Fluorescence

    a Suppression of MCF7 cell growth on the CBC-1 with Accell ESR1 siRNA. Four different Accell siRNA (ESR1-1, ESR1-2, ESR1-3, and ESR1-4) sequences and a pool of all four were tested at 10 μM. Accell nontargeting was used as a negative control. Shown are photomicrographs of merged z-stack images from different wells on the CBC-1 at day 7. Cells per colonies appear as dark dots . b Shown are changes in average MCF7 cell size, and c relative change in colony number between days 2 and 7 normalized to control ( n =5). Asterisk indicates conditions that are significantly different from control ( p

    Journal: Hormones & cancer

    Article Title: The Cancer BioChip System: A Functional Genomic Assay for Anchorage-Independent Three-Dimensional Breast Cancer Cell Growth

    doi: 10.1007/s12672-012-0116-8

    Figure Lengend Snippet: a Suppression of MCF7 cell growth on the CBC-1 with Accell ESR1 siRNA. Four different Accell siRNA (ESR1-1, ESR1-2, ESR1-3, and ESR1-4) sequences and a pool of all four were tested at 10 μM. Accell nontargeting was used as a negative control. Shown are photomicrographs of merged z-stack images from different wells on the CBC-1 at day 7. Cells per colonies appear as dark dots . b Shown are changes in average MCF7 cell size, and c relative change in colony number between days 2 and 7 normalized to control ( n =5). Asterisk indicates conditions that are significantly different from control ( p

    Article Snippet: Accell siRNA (Thermo Fisher Dharmacon, Lafayette, CO) or Mission TRC shRNA-expressing lentiviral vectors (Sigma, Saint Louis, MO) were employed in this study.

    Techniques: Negative Control

    Ubiquitin receptor binding is reduced after CXCR4 gene silencing and CXCR4 ligands compete with ubiquitin for receptor-binding sites. A , CXCR4 was silenced with siRNA in THP1 cells followed by immunoblotting of whole cell lysates with anti-CXCR4 and anti-β-actin.

    Journal: The Journal of Biological Chemistry

    Article Title: CXC Chemokine Receptor 4 Is a Cell Surface Receptor for Extracellular Ubiquitin *

    doi: 10.1074/jbc.M110.103408

    Figure Lengend Snippet: Ubiquitin receptor binding is reduced after CXCR4 gene silencing and CXCR4 ligands compete with ubiquitin for receptor-binding sites. A , CXCR4 was silenced with siRNA in THP1 cells followed by immunoblotting of whole cell lysates with anti-CXCR4 and anti-β-actin.

    Article Snippet: Commercially available Accell CXCR4 siRNA (Thermo Scientific Dharmacon) was reconstituted with 1× siRNA buffer (Thermo Scientific Dharmacon) to a stock concentration of 100 μ m .

    Techniques: Binding Assay

    NFAT5-knockdown attenuates osmolality-induced MCP-1 expression. Met5A cells were transfected with siRNA constructs for NFAT5 or with nontargeting siRNA as control as indicated. Cells were kept in isosmotic medium (gray column; 300 mosm/kg H 2 O) or were exposed to hyperosmotic medium (black column; 400 mosm/kg H 2 O). Medium osmolality was elevated by addition of glucose or NaCl as indicated, and cells were incubated for 24 h. (a) To demonstrate efficiency of NFAT5 knockdown, cells were processed for immunoblotting as described in Section 2 . To demonstrate comparable protein loading, the blots were also probed for actin. (b) For determination of MCP-1 secretion, medium samples were collected and the concentration of MCP-1 in the cell culture supernatant was determined by ELISA as described in Section 2 . Means ± SEM for n = 4 per point; * P

    Journal: Mediators of Inflammation

    Article Title: NFAT5 Contributes to Osmolality-Induced MCP-1 Expression in Mesothelial Cells

    doi: 10.1155/2012/513015

    Figure Lengend Snippet: NFAT5-knockdown attenuates osmolality-induced MCP-1 expression. Met5A cells were transfected with siRNA constructs for NFAT5 or with nontargeting siRNA as control as indicated. Cells were kept in isosmotic medium (gray column; 300 mosm/kg H 2 O) or were exposed to hyperosmotic medium (black column; 400 mosm/kg H 2 O). Medium osmolality was elevated by addition of glucose or NaCl as indicated, and cells were incubated for 24 h. (a) To demonstrate efficiency of NFAT5 knockdown, cells were processed for immunoblotting as described in Section 2 . To demonstrate comparable protein loading, the blots were also probed for actin. (b) For determination of MCP-1 secretion, medium samples were collected and the concentration of MCP-1 in the cell culture supernatant was determined by ELISA as described in Section 2 . Means ± SEM for n = 4 per point; * P

    Article Snippet: NFAT5 Knockdown Accell SMARTpool siRNA construct for knockdown of NFAT5 or Accell nontargeting siRNA (no. 2) were obtained from Thermo Fisher Scientific (Epsom, UK).

    Techniques: Expressing, Transfection, Construct, Incubation, Concentration Assay, Cell Culture, Enzyme-linked Immunosorbent Assay

    ABCA1 interactions with BTN3A1, apoA-I and IPP. ( a ) ABCA1 and BTN3A1 co-immunoprecipitate in untreated and ZA-treated DC U937 and DC but not in CD14+ cells, and ZA treatment does not modify BTN3A1 expression. Pancadherin is employed as a control of equal protein loading (one out of three blots). ( b ) PLA of ABCA1-BTN3A1 interaction by confocal laser-scanning microscopy (ocular lens: × 10; objective: × 63). Ctrl: cells without primary antibodies. Scale bar, 10 μm; blue: nuclear staining (DAPI); green: ABCA1/BTN3A1 interaction (one out of the three experiments). ( c ) apoA-I is physically associated with ABCA1, not with BTN3A1. The expected molecular weight of apoA-I, ABCA1/apoA-I and BTN3A1/apoA-I are shown (one out of the three experiments). ( d ) ABCA1 and BTN3A1 expression in DC after incubation with siRNA for Abca1 (siABca1), Btn3a1 (siBtn3a1) or with scrambled non-targeting siRNA (scr). β-tubulin was employed as control of equal protein loading ( n =3). ( e ) IPP is physically associated with ABCA1 in untreated and ZA-treated DC. The two bands of 150 and 100 kDa in the dithiothreitol (DTT)- and trypsin-treated DC lane are detected with an antibody specific for the N-terminal extracellular loop of ABCA1 in immunoblotting (IB; left). Autoradiography signal of the IPP-ABCA1 interaction (right). Pancadherin is employed as a control of equal protein loading (one out of three experiments). ( f ) Extracellular IPP release in Abca1- and/or Btn3a 1-silenced DC left untreated (ZA-) or after ZA treatment (ZA+). The bars represent the mean±s.e.m. of three experiments (** P

    Journal: Nature Communications

    Article Title: The ATP-binding cassette transporter A1 regulates phosphoantigen release and Vγ9Vδ2 T cell activation by dendritic cells

    doi: 10.1038/ncomms15663

    Figure Lengend Snippet: ABCA1 interactions with BTN3A1, apoA-I and IPP. ( a ) ABCA1 and BTN3A1 co-immunoprecipitate in untreated and ZA-treated DC U937 and DC but not in CD14+ cells, and ZA treatment does not modify BTN3A1 expression. Pancadherin is employed as a control of equal protein loading (one out of three blots). ( b ) PLA of ABCA1-BTN3A1 interaction by confocal laser-scanning microscopy (ocular lens: × 10; objective: × 63). Ctrl: cells without primary antibodies. Scale bar, 10 μm; blue: nuclear staining (DAPI); green: ABCA1/BTN3A1 interaction (one out of the three experiments). ( c ) apoA-I is physically associated with ABCA1, not with BTN3A1. The expected molecular weight of apoA-I, ABCA1/apoA-I and BTN3A1/apoA-I are shown (one out of the three experiments). ( d ) ABCA1 and BTN3A1 expression in DC after incubation with siRNA for Abca1 (siABca1), Btn3a1 (siBtn3a1) or with scrambled non-targeting siRNA (scr). β-tubulin was employed as control of equal protein loading ( n =3). ( e ) IPP is physically associated with ABCA1 in untreated and ZA-treated DC. The two bands of 150 and 100 kDa in the dithiothreitol (DTT)- and trypsin-treated DC lane are detected with an antibody specific for the N-terminal extracellular loop of ABCA1 in immunoblotting (IB; left). Autoradiography signal of the IPP-ABCA1 interaction (right). Pancadherin is employed as a control of equal protein loading (one out of three experiments). ( f ) Extracellular IPP release in Abca1- and/or Btn3a 1-silenced DC left untreated (ZA-) or after ZA treatment (ZA+). The bars represent the mean±s.e.m. of three experiments (** P

    Article Snippet: Abca1 and Btn3a1 silencing Two × 105 DC were transfected with Accell Human siRNA ABCA1, Accell Human BTN3A1 siRNA or 19–25 nucleotide non-targeting scrambled siRNAs (from Thermo Scientific Open Biosystems, Waltham, MA) per the manufacturer's instructions.

    Techniques: Expressing, Proximity Ligation Assay, Confocal Laser Scanning Microscopy, Staining, Molecular Weight, Incubation, Autoradiography