Journal: Mediators of Inflammation
Article Title: The Role of Mcl-1 in S. aureus-Induced Cytoprotection of Infected Macrophages
Figure Lengend Snippet: Effect of Mcl-1 expression on cytoprotection induced by S. aureus in hMDMs. (a) Human macrophages were treated with an MCL1 siRNA (siMCL1) or a nonspecific siRNA (siNS). At 24 h following transfection macrophages were infected with S. aureus at an MOI 1 : 50. After additional 24 h cells were collected and Mcl-1 expression was detected by immunoblot. Data are representative of three separate experiments using hMDMs derived from different donors. (b) The increase in caspase-3 activity (RFU/min) induced by STS in MCL1 knockdown macrophages infected with S. aureus . Twenty-four hours after treatment with siRNA, hMDMs were infected with S. aureus (24 h), followed by treatment with STS at a concentration of 1 μ M for 18 h. The measurement of caspase-3 activity (RFU/min) in cell lysates was performed using DEVD-AFC as a substrate. The figure is representative of three experiments, using hMDMs cultures obtained from different donors. Light bars—STS, dark bars—Sa + STS. Data represent means ± SD. * P
Article Snippet: Transfection with siRNA Silencing of the MCL1 gene expression was accomplished by transfection of cells with specific siRNA or with negative control siRNA (Accell Smart pool and Control pool, resp., Thermo Scientific Dharmacon).
Techniques: Expressing, Transfection, Infection, Derivative Assay, Activity Assay, Concentration Assay