accela pump  (Thermo Fisher)


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    Structured Review

    Thermo Fisher accela pump
    Accela Pump, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accela pump/product/Thermo Fisher
    Average 89 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    accela pump - by Bioz Stars, 2020-07
    89/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Role of Sterylglucosidase 1 (Sgl1) on the pathogenicity of Cryptococcus neoformans: potential applications for vaccine development
    Article Snippet: .. Samples were delivered by Accela pump (Thermo Finnigan, USA) to the HPLC fitted with 3 μm C8SR, 150 mm × 3.0 mm column (Peeke Scientific, Sommerset, NJ, USA). ..

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Immunogenic Properties of Recombinant Enzymes from Bothrops ammodytoides towards the Generation of Neutralizing Antibodies against Its Own Venom
    Article Snippet: .. For rBamMP_1, samples were desalted with Zip Tip C4 (Millipore; Billerica, MA, USA), and injected to an LC–MS (liquid chromatography–mass spectrometry) system composed of an Accela Pump (Thermo-Fisher Co, San Jose, CA, USA) coupled to a LTQ-Orbitrap Velos mass spectrometer (Thermo-Fisher Co., San Jose, CA, USA) with a nano-electrospray (ESI) ionization source. .. The nano-flow liquid chromatography consisted of an isocratic system of 50%–50% of solvents A (water) and B (acetonitrile), both with 0.1% formic acid with a running time of 20 min. A homemade hair needle (ID 0.75 µm and 20 cm long) was used, and the flow of the LC system was 300 nL/min.

    Chromatography:

    Article Title: Selective bacterial degradation of the extracellular matrix attaching the gingiva to the tooth. Selective bacterial degradation of the extracellular matrix attaching the gingiva to the tooth
    Article Snippet: .. Top‐down liquid chromatography/tandem mass spectrometry Before and after incubation with P. gingivalis , samples were diluted in 25% acetonitrile (ACN)/0.3% trifluoroacetic acid and loaded onto a 50 × 4.6 mm PLRP‐S 300A column (Agilent Technologies, Santa Clara, CA, USA) connected to an Accela pump (Thermo Scientific, Waltham, MA, USA) and an RTC autosampler (Pal Systems, Zwingen, Switzerland). .. The buffers used for chromatography were 0.1% formic acid (buffer A) and 100% ACN/0.1% formic acid (buffer B).

    Article Title: A Resveratrol Analogue Promotes ERKMAPK
    Article Snippet: .. Liquid chromatography–mass spectrometry analysis was carried out on a Q-Exactive mass spectrometer coupled to an HTC autosampler and Accela pump (all from Thermo Fisher Scientific, Waltham, MA). .. Twenty-five microliters of the standard or extracts were injected onto an Ascentis Express C18 column (15 × 3 mm; 2.7 µ m; Supelco, Sigma-Aldrich).

    Article Title: Immunogenic Properties of Recombinant Enzymes from Bothrops ammodytoides towards the Generation of Neutralizing Antibodies against Its Own Venom
    Article Snippet: .. For rBamMP_1, samples were desalted with Zip Tip C4 (Millipore; Billerica, MA, USA), and injected to an LC–MS (liquid chromatography–mass spectrometry) system composed of an Accela Pump (Thermo-Fisher Co, San Jose, CA, USA) coupled to a LTQ-Orbitrap Velos mass spectrometer (Thermo-Fisher Co., San Jose, CA, USA) with a nano-electrospray (ESI) ionization source. .. The nano-flow liquid chromatography consisted of an isocratic system of 50%–50% of solvents A (water) and B (acetonitrile), both with 0.1% formic acid with a running time of 20 min. A homemade hair needle (ID 0.75 µm and 20 cm long) was used, and the flow of the LC system was 300 nL/min.

    Incubation:

    Article Title: Selective bacterial degradation of the extracellular matrix attaching the gingiva to the tooth. Selective bacterial degradation of the extracellular matrix attaching the gingiva to the tooth
    Article Snippet: .. Top‐down liquid chromatography/tandem mass spectrometry Before and after incubation with P. gingivalis , samples were diluted in 25% acetonitrile (ACN)/0.3% trifluoroacetic acid and loaded onto a 50 × 4.6 mm PLRP‐S 300A column (Agilent Technologies, Santa Clara, CA, USA) connected to an Accela pump (Thermo Scientific, Waltham, MA, USA) and an RTC autosampler (Pal Systems, Zwingen, Switzerland). .. The buffers used for chromatography were 0.1% formic acid (buffer A) and 100% ACN/0.1% formic acid (buffer B).

    Mass Spectrometry:

    Article Title: A Metabolic Profiling Strategy for the Dissection of Plant Defense against Fungal Pathogens
    Article Snippet: .. The analyzer was equipped with a quadrupole linear ion trap, an Orbitrap electrostatic Fourier transform mass spectrometer (FTMS) with a heated electrospray ionization probe (HESI-II, Thermo Scientific), and an Accela pump (Thermo Scientific). ..

    Article Title: Selective bacterial degradation of the extracellular matrix attaching the gingiva to the tooth. Selective bacterial degradation of the extracellular matrix attaching the gingiva to the tooth
    Article Snippet: .. Top‐down liquid chromatography/tandem mass spectrometry Before and after incubation with P. gingivalis , samples were diluted in 25% acetonitrile (ACN)/0.3% trifluoroacetic acid and loaded onto a 50 × 4.6 mm PLRP‐S 300A column (Agilent Technologies, Santa Clara, CA, USA) connected to an Accela pump (Thermo Scientific, Waltham, MA, USA) and an RTC autosampler (Pal Systems, Zwingen, Switzerland). .. The buffers used for chromatography were 0.1% formic acid (buffer A) and 100% ACN/0.1% formic acid (buffer B).

    Article Title: A Resveratrol Analogue Promotes ERKMAPK
    Article Snippet: .. Liquid chromatography–mass spectrometry analysis was carried out on a Q-Exactive mass spectrometer coupled to an HTC autosampler and Accela pump (all from Thermo Fisher Scientific, Waltham, MA). .. Twenty-five microliters of the standard or extracts were injected onto an Ascentis Express C18 column (15 × 3 mm; 2.7 µ m; Supelco, Sigma-Aldrich).

    Article Title: Maternal choline supplementation programs greater activity of the phosphatidylethanolamine N-methyltransferase (PEMT) pathway in adult Ts65Dn trisomic mice
    Article Snippet: .. The LC-MS/MS system consisted of an Accela pump with degasser (Thermo, San Jose, CA, USA), a refrigerated Accela autosampler (Thermo), and a TSQ Quantum mass spectrometer (Thermo), which was equipped with an electrospray ionization source and operated in positive ion mode. .. Aqueous extract (10 μl) was injected onto a Prevail silica column (150×2.1 mm, 5 μm; Grace, Deerfield, IL, USA) with matching guard column (7.5×2.1 mm, 5 μm) for separation of the water-soluble choline metabolites.

    Article Title: Natural Hypolignification Is Associated with Extensive Oligolignol Accumulation in Flax Stems 1Natural Hypolignification Is Associated with Extensive Oligolignol Accumulation in Flax Stems 1 [C]Natural Hypolignification Is Associated with Extensive Oligolignol Accumulation in Flax Stems 1 [C] [W]
    Article Snippet: .. Extracts were analyzed with an Accela UHPLC system (Thermo Electron) consisting of an Accela autosampler coupled to an Accela pump and further hyphenated to a LTQ FT Ultra (Thermo Electron) MS unit consisting of a linear ion-trap mass spectrometer connected with a FT-ICR-MS device. .. The separation was performed on a reverse-phase Acquity UPLC BEH C18 column (150 mm × 2.1 mm, 1.7 μm; Waters) with aqueous 0.1% acetic acid and acetonitrile:water (99:1, v/v; acidified with 0.1% acetic acid) as solvents A and B.

    Article Title: Immunogenic Properties of Recombinant Enzymes from Bothrops ammodytoides towards the Generation of Neutralizing Antibodies against Its Own Venom
    Article Snippet: .. For rBamMP_1, samples were desalted with Zip Tip C4 (Millipore; Billerica, MA, USA), and injected to an LC–MS (liquid chromatography–mass spectrometry) system composed of an Accela Pump (Thermo-Fisher Co, San Jose, CA, USA) coupled to a LTQ-Orbitrap Velos mass spectrometer (Thermo-Fisher Co., San Jose, CA, USA) with a nano-electrospray (ESI) ionization source. .. The nano-flow liquid chromatography consisted of an isocratic system of 50%–50% of solvents A (water) and B (acetonitrile), both with 0.1% formic acid with a running time of 20 min. A homemade hair needle (ID 0.75 µm and 20 cm long) was used, and the flow of the LC system was 300 nL/min.

    Article Title: Betaine improves nonalcoholic fatty liver and associated hepatic insulin resistance: a potential mechanism for hepatoprotection by betaine
    Article Snippet: .. The supernatant (150 μl) was then transferred to a vial from which 10 μl was injected into a LC-MS/MS system consisting of a TSQ Quantum mass spectrometer (Thermo, San Jose, CA) equipped with a refrigerated Accela autosampler (Thermo) and an Accela pump with degasser (Thermo). .. Metabolites were separated by HPLC using a Prevail silica column (150 × 2.1 mm, 5 μm; Grace, Deerfield, IL) with matching guard column (4.6 × 25 mm, 5 μm).

    Injection:

    Article Title: Immunogenic Properties of Recombinant Enzymes from Bothrops ammodytoides towards the Generation of Neutralizing Antibodies against Its Own Venom
    Article Snippet: .. For rBamMP_1, samples were desalted with Zip Tip C4 (Millipore; Billerica, MA, USA), and injected to an LC–MS (liquid chromatography–mass spectrometry) system composed of an Accela Pump (Thermo-Fisher Co, San Jose, CA, USA) coupled to a LTQ-Orbitrap Velos mass spectrometer (Thermo-Fisher Co., San Jose, CA, USA) with a nano-electrospray (ESI) ionization source. .. The nano-flow liquid chromatography consisted of an isocratic system of 50%–50% of solvents A (water) and B (acetonitrile), both with 0.1% formic acid with a running time of 20 min. A homemade hair needle (ID 0.75 µm and 20 cm long) was used, and the flow of the LC system was 300 nL/min.

    Article Title: Betaine improves nonalcoholic fatty liver and associated hepatic insulin resistance: a potential mechanism for hepatoprotection by betaine
    Article Snippet: .. The supernatant (150 μl) was then transferred to a vial from which 10 μl was injected into a LC-MS/MS system consisting of a TSQ Quantum mass spectrometer (Thermo, San Jose, CA) equipped with a refrigerated Accela autosampler (Thermo) and an Accela pump with degasser (Thermo). .. Metabolites were separated by HPLC using a Prevail silica column (150 × 2.1 mm, 5 μm; Grace, Deerfield, IL) with matching guard column (4.6 × 25 mm, 5 μm).

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  • 88
    Thermo Fisher accela 600 pump
    Lipid profiling of the optic nerve (ON) regeneration . ( a ) The dataset consists of the following experimental groups: intact control, ON crush (followed by intravitreal injection of the vehicle) and inflammation-induced ON regeneration (ON crush followed by intravitreal injection of Zymosan + CPT-cAMP). ONs were harvested 3, 7, 14 days post-crush and retinas 7, 14 days post-crush. ( b ) In the longitudinal sections of rat ON, Zymosan + CPT-cAMP increases expression of a marker of axon regeneration, GAP43, distal to the crush site (*). ( c ) Following methanol-chloroform-based extraction, lipids were separated by C30 high-performance liquid chromatography (HPLC) system using <t>Accela</t> 600 pump and measured in (+)/(−) heated electrospray (HESI) ionization mode using a Q Exactive mass spectrometer. Lipidome identification and relative quantification were performed in LipidSearch, followed by statistical analysis in MetaboAnalyst.
    Accela 600 Pump, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accela 600 pump/product/Thermo Fisher
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    accela 600 pump - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    88
    Thermo Fisher accela quaternary hplc pump
    Lipid profiling of the optic nerve (ON) regeneration . ( a ) The dataset consists of the following experimental groups: intact control, ON crush (followed by intravitreal injection of the vehicle) and inflammation-induced ON regeneration (ON crush followed by intravitreal injection of Zymosan + CPT-cAMP). ONs were harvested 3, 7, 14 days post-crush and retinas 7, 14 days post-crush. ( b ) In the longitudinal sections of rat ON, Zymosan + CPT-cAMP increases expression of a marker of axon regeneration, GAP43, distal to the crush site (*). ( c ) Following methanol-chloroform-based extraction, lipids were separated by C30 high-performance liquid chromatography (HPLC) system using <t>Accela</t> 600 pump and measured in (+)/(−) heated electrospray (HESI) ionization mode using a Q Exactive mass spectrometer. Lipidome identification and relative quantification were performed in LipidSearch, followed by statistical analysis in MetaboAnalyst.
    Accela Quaternary Hplc Pump, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accela quaternary hplc pump/product/Thermo Fisher
    Average 88 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    accela quaternary hplc pump - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    90
    Thermo Fisher accela 1250 quaternary pumps
    Lipid profiling of the optic nerve (ON) regeneration . ( a ) The dataset consists of the following experimental groups: intact control, ON crush (followed by intravitreal injection of the vehicle) and inflammation-induced ON regeneration (ON crush followed by intravitreal injection of Zymosan + CPT-cAMP). ONs were harvested 3, 7, 14 days post-crush and retinas 7, 14 days post-crush. ( b ) In the longitudinal sections of rat ON, Zymosan + CPT-cAMP increases expression of a marker of axon regeneration, GAP43, distal to the crush site (*). ( c ) Following methanol-chloroform-based extraction, lipids were separated by C30 high-performance liquid chromatography (HPLC) system using <t>Accela</t> 600 pump and measured in (+)/(−) heated electrospray (HESI) ionization mode using a Q Exactive mass spectrometer. Lipidome identification and relative quantification were performed in LipidSearch, followed by statistical analysis in MetaboAnalyst.
    Accela 1250 Quaternary Pumps, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accela 1250 quaternary pumps/product/Thermo Fisher
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    accela 1250 quaternary pumps - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    Image Search Results


    Lipid profiling of the optic nerve (ON) regeneration . ( a ) The dataset consists of the following experimental groups: intact control, ON crush (followed by intravitreal injection of the vehicle) and inflammation-induced ON regeneration (ON crush followed by intravitreal injection of Zymosan + CPT-cAMP). ONs were harvested 3, 7, 14 days post-crush and retinas 7, 14 days post-crush. ( b ) In the longitudinal sections of rat ON, Zymosan + CPT-cAMP increases expression of a marker of axon regeneration, GAP43, distal to the crush site (*). ( c ) Following methanol-chloroform-based extraction, lipids were separated by C30 high-performance liquid chromatography (HPLC) system using Accela 600 pump and measured in (+)/(−) heated electrospray (HESI) ionization mode using a Q Exactive mass spectrometer. Lipidome identification and relative quantification were performed in LipidSearch, followed by statistical analysis in MetaboAnalyst.

    Journal: Data in Brief

    Article Title: Comparative lipid profiling dataset of the inflammation-induced optic nerve regeneration

    doi: 10.1016/j.dib.2019.103950

    Figure Lengend Snippet: Lipid profiling of the optic nerve (ON) regeneration . ( a ) The dataset consists of the following experimental groups: intact control, ON crush (followed by intravitreal injection of the vehicle) and inflammation-induced ON regeneration (ON crush followed by intravitreal injection of Zymosan + CPT-cAMP). ONs were harvested 3, 7, 14 days post-crush and retinas 7, 14 days post-crush. ( b ) In the longitudinal sections of rat ON, Zymosan + CPT-cAMP increases expression of a marker of axon regeneration, GAP43, distal to the crush site (*). ( c ) Following methanol-chloroform-based extraction, lipids were separated by C30 high-performance liquid chromatography (HPLC) system using Accela 600 pump and measured in (+)/(−) heated electrospray (HESI) ionization mode using a Q Exactive mass spectrometer. Lipidome identification and relative quantification were performed in LipidSearch, followed by statistical analysis in MetaboAnalyst.

    Article Snippet: Reversed-phase chromatographic separation was achieved with the Accela Autosampler, Accela 600 pump and Acclaim C30 column: 3 μm, 2.1 × 150 mm (Thermo Fisher Scientific, Waltham, MA).

    Techniques: Injection, Cycling Probe Technology, Expressing, Marker, High Performance Liquid Chromatography, Mass Spectrometry