accela hplc pump  (Thermo Fisher)


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    Structured Review

    Thermo Fisher accela hplc pump
    Accela Hplc Pump, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accela hplc pump/product/Thermo Fisher
    Average 88 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    accela hplc pump - by Bioz Stars, 2020-07
    88/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: The Roles of Sphingosine Kinase 1 and 2 in Regulating the Metabolome and Survival of Prostate Cancer Cells
    Article Snippet: .. The chromatographic conditions were as follows: A ZICpHILIC column (150 × 4.6 mm × 5 µm) was eluted with a linear gradient over 30 min between 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (20:80) at 0 min and 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (20:80) at 30 min with a flow rate of 0.3 mL/min, followed by washing with 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (95:5) for 5 min and then re-equilibration with the starting conditions for 10 min. LC/MS was carried out by using an Accela HPLC pump coupled to an Exactive (Orbitrap) mass spectrometer from Thermo Fisher Scientific (Bremen, Germany). ..

    Article Title: The roles of sphingosine kinase 1 and 2 in regulating the Warburg effect in prostate cancer cells
    Article Snippet: .. The chromatographic conditions were as follows: A ZICpHILIC column (150×4.6 mm×5 µm) was eluted with a linear gradient over 30 min between 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (20:80) at 0 min and 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (20:80) at 30 min with a flow rate of 0.3 ml/min, followed by washing with 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (95:5) for 5 min and then re-equilibration with the starting conditions for 10 min. LC/MS was carried out by using an Accela HPLC pump coupled to an Exactive (Orbitrap) mass spectrometer from Thermo Fisher Scientific (Bremen, Germany). ..

    Article Title: The sphingosine kinase inhibitor 2-(p-hyroxyanilino)-4-(p-chlorophenyl) thiazole reduces androgen receptor expression via an oxidative stress-dependent mechanism
    Article Snippet: .. LC-MS analysis was carried out using a ZICpHILIC column (150 × 4.6 mm × 5 μm; HiChrom, Reading, UK), which was eluted with a linear gradient over 30 min between 20 mM ammonium carbonate (pH 9.2)/acetonitrile (20:80) at 0 min and 20 mM ammonium carbonate (pH 9.2)/acetonitrile (20:80) at 30 min with a flow rate of 0.3 mL·min−1 , followed by washing with 20 mM ammonium carbonate (pH 9.2)/acetonitrile (95:5) for 5 min and then re-equilibrated with the starting conditions for 10 min. LC-MS was carried out with an Accela HPLC pump coupled to an Exactive (Orbitrap) mass spectrometer from Thermo Fisher Scientific (Bremen, Germany). .. Nitrogen as sheath and auxiliary gas was produced by a NM32LA nitrogen generator from Peak Scientific (Scotland, UK).

    Article Title: Overexpression of the UGT73C6 alters brassinosteroid glucoside formation in Arabidopsis thaliana
    Article Snippet: .. An Accela HPLC pump (Thermo Fisher Scientific, Waltham, MA, USA) together with a Mistral column thermostat (Maylab, Thermo Fisher Scientific) and a PAL HTC autosampler (CTC Analytics, Zwingen, Switzerland) were coupled to a LTQ Orbitrap XL high-resolution mass spectrometer (Thermo Fisher Scientific). .. Separation was performed on a Hypersil Gold column (150 × 2.1 mm, 3 μm particle size; Thermo Fisher Scientific) at 25°C.

    Article Title: Perfluorinated substances, risk factors for multiple sclerosis and cellular immune activation
    Article Snippet: .. The extraction was performed on a Thermo Scientific EQuan MAX system, consisting of two Accela HPLC pump (Thermo Scientific, San José, CA) and a PAL autosampler module (CTC analysis AG, Zwingen, Switzerland). .. The tandem mass spectrometer was a TSQ Quantum Ultra Triple Stage Quadropole with heated electrospray ionization (Thermo Scientific, San Jose, CA).

    Article Title: Proteomic analysis identifies highly expressed plasma membrane proteins for detection and therapeutic targeting of specific breast cancer subtypes
    Article Snippet: .. MudPIT analysis was performed using an Accela HPLC pump (Thermo) and LTQ XL (Thermo) using an in-house built electrospray stage [ ]. .. Protein and peptide identification were done with Integrated Proteomics Pipeline—IP2 (Integrated Proteomics Applications, San Diego, CA. http://www.integratedproteomics.com/ ).

    Article Title: Plasma Membrane Proteomics of Human Breast Cancer Cell Lines Identifies Potential Targets for Breast Cancer Diagnosis and Treatment
    Article Snippet: .. LC-MS/MS analysis was performed using an Accela HPLC pump (Thermo) and LTQ XL (Thermo) using an in-house built electrospray stage. .. Electrospray was performed directly from the analytical column by applying the ESI voltage at a tee (150 µm ID, Upchurch Scientific) directly downstream of a 1∶1000 split flow used to reduce the flow rate to 300 nL/min through the columns.

    Article Title: Phosphothreonine 218 is required for the function of SR45.1 in regulating flower petal development in Arabidopsis
    Article Snippet: .. Peptides were separated on a C18 reverse phase column (100 x 0.18 mm BioBasic-18) using a linear gradient from 5% to 40% acetonitrile/0.1% formic acid at a flow rate of 3 mL/min which was controlled by an Accela HPLC pump (Thermo Fisher Scientific, Waltham, MA). .. The eluent was electrosprayed at 3.5 kV directly into the orifice of an LTQ-Orbitrap XL mass spectrometer (Thermo Fisher Scientific) controlled by Xcalibur 2.0.7 software (Thermo Fisher Scientific).

    Flow Cytometry:

    Article Title: The Roles of Sphingosine Kinase 1 and 2 in Regulating the Metabolome and Survival of Prostate Cancer Cells
    Article Snippet: .. The chromatographic conditions were as follows: A ZICpHILIC column (150 × 4.6 mm × 5 µm) was eluted with a linear gradient over 30 min between 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (20:80) at 0 min and 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (20:80) at 30 min with a flow rate of 0.3 mL/min, followed by washing with 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (95:5) for 5 min and then re-equilibration with the starting conditions for 10 min. LC/MS was carried out by using an Accela HPLC pump coupled to an Exactive (Orbitrap) mass spectrometer from Thermo Fisher Scientific (Bremen, Germany). ..

    Article Title: The roles of sphingosine kinase 1 and 2 in regulating the Warburg effect in prostate cancer cells
    Article Snippet: .. The chromatographic conditions were as follows: A ZICpHILIC column (150×4.6 mm×5 µm) was eluted with a linear gradient over 30 min between 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (20:80) at 0 min and 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (20:80) at 30 min with a flow rate of 0.3 ml/min, followed by washing with 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (95:5) for 5 min and then re-equilibration with the starting conditions for 10 min. LC/MS was carried out by using an Accela HPLC pump coupled to an Exactive (Orbitrap) mass spectrometer from Thermo Fisher Scientific (Bremen, Germany). ..

    Article Title: The sphingosine kinase inhibitor 2-(p-hyroxyanilino)-4-(p-chlorophenyl) thiazole reduces androgen receptor expression via an oxidative stress-dependent mechanism
    Article Snippet: .. LC-MS analysis was carried out using a ZICpHILIC column (150 × 4.6 mm × 5 μm; HiChrom, Reading, UK), which was eluted with a linear gradient over 30 min between 20 mM ammonium carbonate (pH 9.2)/acetonitrile (20:80) at 0 min and 20 mM ammonium carbonate (pH 9.2)/acetonitrile (20:80) at 30 min with a flow rate of 0.3 mL·min−1 , followed by washing with 20 mM ammonium carbonate (pH 9.2)/acetonitrile (95:5) for 5 min and then re-equilibrated with the starting conditions for 10 min. LC-MS was carried out with an Accela HPLC pump coupled to an Exactive (Orbitrap) mass spectrometer from Thermo Fisher Scientific (Bremen, Germany). .. Nitrogen as sheath and auxiliary gas was produced by a NM32LA nitrogen generator from Peak Scientific (Scotland, UK).

    Article Title: Phosphothreonine 218 is required for the function of SR45.1 in regulating flower petal development in Arabidopsis
    Article Snippet: .. Peptides were separated on a C18 reverse phase column (100 x 0.18 mm BioBasic-18) using a linear gradient from 5% to 40% acetonitrile/0.1% formic acid at a flow rate of 3 mL/min which was controlled by an Accela HPLC pump (Thermo Fisher Scientific, Waltham, MA). .. The eluent was electrosprayed at 3.5 kV directly into the orifice of an LTQ-Orbitrap XL mass spectrometer (Thermo Fisher Scientific) controlled by Xcalibur 2.0.7 software (Thermo Fisher Scientific).

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: The Roles of Sphingosine Kinase 1 and 2 in Regulating the Metabolome and Survival of Prostate Cancer Cells
    Article Snippet: .. The chromatographic conditions were as follows: A ZICpHILIC column (150 × 4.6 mm × 5 µm) was eluted with a linear gradient over 30 min between 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (20:80) at 0 min and 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (20:80) at 30 min with a flow rate of 0.3 mL/min, followed by washing with 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (95:5) for 5 min and then re-equilibration with the starting conditions for 10 min. LC/MS was carried out by using an Accela HPLC pump coupled to an Exactive (Orbitrap) mass spectrometer from Thermo Fisher Scientific (Bremen, Germany). ..

    Article Title: The roles of sphingosine kinase 1 and 2 in regulating the Warburg effect in prostate cancer cells
    Article Snippet: .. The chromatographic conditions were as follows: A ZICpHILIC column (150×4.6 mm×5 µm) was eluted with a linear gradient over 30 min between 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (20:80) at 0 min and 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (20:80) at 30 min with a flow rate of 0.3 ml/min, followed by washing with 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (95:5) for 5 min and then re-equilibration with the starting conditions for 10 min. LC/MS was carried out by using an Accela HPLC pump coupled to an Exactive (Orbitrap) mass spectrometer from Thermo Fisher Scientific (Bremen, Germany). ..

    Article Title: The sphingosine kinase inhibitor 2-(p-hyroxyanilino)-4-(p-chlorophenyl) thiazole reduces androgen receptor expression via an oxidative stress-dependent mechanism
    Article Snippet: .. LC-MS analysis was carried out using a ZICpHILIC column (150 × 4.6 mm × 5 μm; HiChrom, Reading, UK), which was eluted with a linear gradient over 30 min between 20 mM ammonium carbonate (pH 9.2)/acetonitrile (20:80) at 0 min and 20 mM ammonium carbonate (pH 9.2)/acetonitrile (20:80) at 30 min with a flow rate of 0.3 mL·min−1 , followed by washing with 20 mM ammonium carbonate (pH 9.2)/acetonitrile (95:5) for 5 min and then re-equilibrated with the starting conditions for 10 min. LC-MS was carried out with an Accela HPLC pump coupled to an Exactive (Orbitrap) mass spectrometer from Thermo Fisher Scientific (Bremen, Germany). .. Nitrogen as sheath and auxiliary gas was produced by a NM32LA nitrogen generator from Peak Scientific (Scotland, UK).

    Mass Spectrometry:

    Article Title: The Roles of Sphingosine Kinase 1 and 2 in Regulating the Metabolome and Survival of Prostate Cancer Cells
    Article Snippet: .. The chromatographic conditions were as follows: A ZICpHILIC column (150 × 4.6 mm × 5 µm) was eluted with a linear gradient over 30 min between 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (20:80) at 0 min and 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (20:80) at 30 min with a flow rate of 0.3 mL/min, followed by washing with 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (95:5) for 5 min and then re-equilibration with the starting conditions for 10 min. LC/MS was carried out by using an Accela HPLC pump coupled to an Exactive (Orbitrap) mass spectrometer from Thermo Fisher Scientific (Bremen, Germany). ..

    Article Title: The roles of sphingosine kinase 1 and 2 in regulating the Warburg effect in prostate cancer cells
    Article Snippet: .. The chromatographic conditions were as follows: A ZICpHILIC column (150×4.6 mm×5 µm) was eluted with a linear gradient over 30 min between 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (20:80) at 0 min and 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (20:80) at 30 min with a flow rate of 0.3 ml/min, followed by washing with 20 mM (NH4 )2 CO3 (pH 9.2)/MeCN (95:5) for 5 min and then re-equilibration with the starting conditions for 10 min. LC/MS was carried out by using an Accela HPLC pump coupled to an Exactive (Orbitrap) mass spectrometer from Thermo Fisher Scientific (Bremen, Germany). ..

    Article Title: The sphingosine kinase inhibitor 2-(p-hyroxyanilino)-4-(p-chlorophenyl) thiazole reduces androgen receptor expression via an oxidative stress-dependent mechanism
    Article Snippet: .. LC-MS analysis was carried out using a ZICpHILIC column (150 × 4.6 mm × 5 μm; HiChrom, Reading, UK), which was eluted with a linear gradient over 30 min between 20 mM ammonium carbonate (pH 9.2)/acetonitrile (20:80) at 0 min and 20 mM ammonium carbonate (pH 9.2)/acetonitrile (20:80) at 30 min with a flow rate of 0.3 mL·min−1 , followed by washing with 20 mM ammonium carbonate (pH 9.2)/acetonitrile (95:5) for 5 min and then re-equilibrated with the starting conditions for 10 min. LC-MS was carried out with an Accela HPLC pump coupled to an Exactive (Orbitrap) mass spectrometer from Thermo Fisher Scientific (Bremen, Germany). .. Nitrogen as sheath and auxiliary gas was produced by a NM32LA nitrogen generator from Peak Scientific (Scotland, UK).

    Article Title: Overexpression of the UGT73C6 alters brassinosteroid glucoside formation in Arabidopsis thaliana
    Article Snippet: .. An Accela HPLC pump (Thermo Fisher Scientific, Waltham, MA, USA) together with a Mistral column thermostat (Maylab, Thermo Fisher Scientific) and a PAL HTC autosampler (CTC Analytics, Zwingen, Switzerland) were coupled to a LTQ Orbitrap XL high-resolution mass spectrometer (Thermo Fisher Scientific). .. Separation was performed on a Hypersil Gold column (150 × 2.1 mm, 3 μm particle size; Thermo Fisher Scientific) at 25°C.

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  • 99
    Thermo Fisher hplc system
    Functional characterization of the PKS19 cluster, a putative polyketide biosynthetic gene cluster that is unique to F. fujikuroi . (A) Position and organization of the PKS19 gene cluster on F. fujikuroi chromosome VIII, GC content, distribution of active histone marks, and gene expression in the PKS19 cluster. Histone marks are described in the legend for Figure 3 . Expression data are from microarray analysis of wild-type F. fujikuroi (strain IMI58289). Values are log 2 change in expression in a high versus low-nitrogen medium. H3K9ac and gene expression are correlated, as both are increased in the high-nitrogen medium. Some genes exhibited increased levels of H3K4me2 in the high-nitrogen medium, which also suggests transcription. (B) Chemical analysis of the SM product(s) of the PKS19 cluster. The traces show the combined extracted ion chromatograms for metabolites with molecular formulas [C 12 H 16 O 4 +H] + , [C 12 H 18 O 5 +H] + and [C 12 H 18 O 4 +H] + determined by <t>HPLC-FTMS</t> of culture fluids from F. fujikuroi strains: IMI58289, wild-type strain; OE::TF, a strain over-expressing the transcription factor encoded by FFUJ_12242; OE::PKS19, a strain over-expressing the PKS19 gene FFUJ_12239; and OE::TF/OE::PKS19, a strain over-expressing both FFUJ-12242 and FFUJ_12239. (C) Northern blot analysis of PKS19 cluster genes in strains IMI58289 (WT), OE::TF, OE::PKS19 and OE::TF/OE::PKS19. (D) UV spectra of metabolites corresponding to peaks 1 through 4 from chromatograms shown in C. The similar spectra of the metabolites suggest structural similarity.
    Hplc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 587 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc system/product/Thermo Fisher
    Average 99 stars, based on 587 article reviews
    Price from $9.99 to $1999.99
    hplc system - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    88
    Thermo Fisher accela quaternary hplc pump
    Functional characterization of the PKS19 cluster, a putative polyketide biosynthetic gene cluster that is unique to F. fujikuroi . (A) Position and organization of the PKS19 gene cluster on F. fujikuroi chromosome VIII, GC content, distribution of active histone marks, and gene expression in the PKS19 cluster. Histone marks are described in the legend for Figure 3 . Expression data are from microarray analysis of wild-type F. fujikuroi (strain IMI58289). Values are log 2 change in expression in a high versus low-nitrogen medium. H3K9ac and gene expression are correlated, as both are increased in the high-nitrogen medium. Some genes exhibited increased levels of H3K4me2 in the high-nitrogen medium, which also suggests transcription. (B) Chemical analysis of the SM product(s) of the PKS19 cluster. The traces show the combined extracted ion chromatograms for metabolites with molecular formulas [C 12 H 16 O 4 +H] + , [C 12 H 18 O 5 +H] + and [C 12 H 18 O 4 +H] + determined by <t>HPLC-FTMS</t> of culture fluids from F. fujikuroi strains: IMI58289, wild-type strain; OE::TF, a strain over-expressing the transcription factor encoded by FFUJ_12242; OE::PKS19, a strain over-expressing the PKS19 gene FFUJ_12239; and OE::TF/OE::PKS19, a strain over-expressing both FFUJ-12242 and FFUJ_12239. (C) Northern blot analysis of PKS19 cluster genes in strains IMI58289 (WT), OE::TF, OE::PKS19 and OE::TF/OE::PKS19. (D) UV spectra of metabolites corresponding to peaks 1 through 4 from chromatograms shown in C. The similar spectra of the metabolites suggest structural similarity.
    Accela Quaternary Hplc Pump, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accela quaternary hplc pump/product/Thermo Fisher
    Average 88 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    accela quaternary hplc pump - by Bioz Stars, 2020-07
    88/100 stars
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    90
    Thermo Fisher high pressure quaternary pump accela 1250
    Functional characterization of the PKS19 cluster, a putative polyketide biosynthetic gene cluster that is unique to F. fujikuroi . (A) Position and organization of the PKS19 gene cluster on F. fujikuroi chromosome VIII, GC content, distribution of active histone marks, and gene expression in the PKS19 cluster. Histone marks are described in the legend for Figure 3 . Expression data are from microarray analysis of wild-type F. fujikuroi (strain IMI58289). Values are log 2 change in expression in a high versus low-nitrogen medium. H3K9ac and gene expression are correlated, as both are increased in the high-nitrogen medium. Some genes exhibited increased levels of H3K4me2 in the high-nitrogen medium, which also suggests transcription. (B) Chemical analysis of the SM product(s) of the PKS19 cluster. The traces show the combined extracted ion chromatograms for metabolites with molecular formulas [C 12 H 16 O 4 +H] + , [C 12 H 18 O 5 +H] + and [C 12 H 18 O 4 +H] + determined by <t>HPLC-FTMS</t> of culture fluids from F. fujikuroi strains: IMI58289, wild-type strain; OE::TF, a strain over-expressing the transcription factor encoded by FFUJ_12242; OE::PKS19, a strain over-expressing the PKS19 gene FFUJ_12239; and OE::TF/OE::PKS19, a strain over-expressing both FFUJ-12242 and FFUJ_12239. (C) Northern blot analysis of PKS19 cluster genes in strains IMI58289 (WT), OE::TF, OE::PKS19 and OE::TF/OE::PKS19. (D) UV spectra of metabolites corresponding to peaks 1 through 4 from chromatograms shown in C. The similar spectra of the metabolites suggest structural similarity.
    High Pressure Quaternary Pump Accela 1250, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high pressure quaternary pump accela 1250/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high pressure quaternary pump accela 1250 - by Bioz Stars, 2020-07
    90/100 stars
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    Functional characterization of the PKS19 cluster, a putative polyketide biosynthetic gene cluster that is unique to F. fujikuroi . (A) Position and organization of the PKS19 gene cluster on F. fujikuroi chromosome VIII, GC content, distribution of active histone marks, and gene expression in the PKS19 cluster. Histone marks are described in the legend for Figure 3 . Expression data are from microarray analysis of wild-type F. fujikuroi (strain IMI58289). Values are log 2 change in expression in a high versus low-nitrogen medium. H3K9ac and gene expression are correlated, as both are increased in the high-nitrogen medium. Some genes exhibited increased levels of H3K4me2 in the high-nitrogen medium, which also suggests transcription. (B) Chemical analysis of the SM product(s) of the PKS19 cluster. The traces show the combined extracted ion chromatograms for metabolites with molecular formulas [C 12 H 16 O 4 +H] + , [C 12 H 18 O 5 +H] + and [C 12 H 18 O 4 +H] + determined by HPLC-FTMS of culture fluids from F. fujikuroi strains: IMI58289, wild-type strain; OE::TF, a strain over-expressing the transcription factor encoded by FFUJ_12242; OE::PKS19, a strain over-expressing the PKS19 gene FFUJ_12239; and OE::TF/OE::PKS19, a strain over-expressing both FFUJ-12242 and FFUJ_12239. (C) Northern blot analysis of PKS19 cluster genes in strains IMI58289 (WT), OE::TF, OE::PKS19 and OE::TF/OE::PKS19. (D) UV spectra of metabolites corresponding to peaks 1 through 4 from chromatograms shown in C. The similar spectra of the metabolites suggest structural similarity.

    Journal: PLoS Pathogens

    Article Title: Deciphering the Cryptic Genome: Genome-wide Analyses of the Rice Pathogen Fusarium fujikuroi Reveal Complex Regulation of Secondary Metabolism and Novel Metabolites

    doi: 10.1371/journal.ppat.1003475

    Figure Lengend Snippet: Functional characterization of the PKS19 cluster, a putative polyketide biosynthetic gene cluster that is unique to F. fujikuroi . (A) Position and organization of the PKS19 gene cluster on F. fujikuroi chromosome VIII, GC content, distribution of active histone marks, and gene expression in the PKS19 cluster. Histone marks are described in the legend for Figure 3 . Expression data are from microarray analysis of wild-type F. fujikuroi (strain IMI58289). Values are log 2 change in expression in a high versus low-nitrogen medium. H3K9ac and gene expression are correlated, as both are increased in the high-nitrogen medium. Some genes exhibited increased levels of H3K4me2 in the high-nitrogen medium, which also suggests transcription. (B) Chemical analysis of the SM product(s) of the PKS19 cluster. The traces show the combined extracted ion chromatograms for metabolites with molecular formulas [C 12 H 16 O 4 +H] + , [C 12 H 18 O 5 +H] + and [C 12 H 18 O 4 +H] + determined by HPLC-FTMS of culture fluids from F. fujikuroi strains: IMI58289, wild-type strain; OE::TF, a strain over-expressing the transcription factor encoded by FFUJ_12242; OE::PKS19, a strain over-expressing the PKS19 gene FFUJ_12239; and OE::TF/OE::PKS19, a strain over-expressing both FFUJ-12242 and FFUJ_12239. (C) Northern blot analysis of PKS19 cluster genes in strains IMI58289 (WT), OE::TF, OE::PKS19 and OE::TF/OE::PKS19. (D) UV spectra of metabolites corresponding to peaks 1 through 4 from chromatograms shown in C. The similar spectra of the metabolites suggest structural similarity.

    Article Snippet: HPLC-FTMS The HPLC-FTMS system used an HPLC system (Accela LC with Accela Pump 60057-60010 and Accela Autosampler 60057-60020, Thermo Scientific, Dreieich, Germany) coupled to a Fourier transform mass spectrometer with a heated ESI source (LTQ Orbitrap XL, Thermo Scientific, Dreieich, Germany).

    Techniques: Functional Assay, Expressing, Microarray, High Performance Liquid Chromatography, Northern Blot

    Location of the NRPS/APS biosynthetic gene cluster on F. fujikuroi chromosome I, levels of histone modifications and gene expression within and flanking the cluster, and production of metabolites following overexpression of cluster genes APS2 and APS8 . A : Synteny between the apicidin gene cluster in F. semitectum [105] and the apicidin-like gene cluster in F. fujikuroi . B : Histone modifications and gene expression in and flanking the cluster. Histone marks are described in the legend to Figure 3 . Expression data were derived from microarray experiments in low and high nitrogen and are plotted as the changes in log 2 expression values in high-nitrogen medium compared to low-nitrogen medium. H3K9ac and gene expression are overall correlated, as both are increased under high nitrogen conditions. In some genes increased H3K4me2 was observed, also suggesting transcription. C : Chemical analysis of the product of the unique PKS19 gene cluster. The traces show the extracted ion chromatograms for [C 34 H 48 O 6 N 5 +H] + (first line) and [C 35 H 42 O 7 N 5 +H] + (second to fourth line) determined by HPLC-FTMS of an apicidin standard (first line) and of culture fluids from F. fujikuroi IMI58289, OE::APS8 and the OE::APS2/OE::APS8 mutant. D : UV spectra of apicidin and the apicidin-like compound. The similar spectra suggest a structural similarity.

    Journal: PLoS Pathogens

    Article Title: Deciphering the Cryptic Genome: Genome-wide Analyses of the Rice Pathogen Fusarium fujikuroi Reveal Complex Regulation of Secondary Metabolism and Novel Metabolites

    doi: 10.1371/journal.ppat.1003475

    Figure Lengend Snippet: Location of the NRPS/APS biosynthetic gene cluster on F. fujikuroi chromosome I, levels of histone modifications and gene expression within and flanking the cluster, and production of metabolites following overexpression of cluster genes APS2 and APS8 . A : Synteny between the apicidin gene cluster in F. semitectum [105] and the apicidin-like gene cluster in F. fujikuroi . B : Histone modifications and gene expression in and flanking the cluster. Histone marks are described in the legend to Figure 3 . Expression data were derived from microarray experiments in low and high nitrogen and are plotted as the changes in log 2 expression values in high-nitrogen medium compared to low-nitrogen medium. H3K9ac and gene expression are overall correlated, as both are increased under high nitrogen conditions. In some genes increased H3K4me2 was observed, also suggesting transcription. C : Chemical analysis of the product of the unique PKS19 gene cluster. The traces show the extracted ion chromatograms for [C 34 H 48 O 6 N 5 +H] + (first line) and [C 35 H 42 O 7 N 5 +H] + (second to fourth line) determined by HPLC-FTMS of an apicidin standard (first line) and of culture fluids from F. fujikuroi IMI58289, OE::APS8 and the OE::APS2/OE::APS8 mutant. D : UV spectra of apicidin and the apicidin-like compound. The similar spectra suggest a structural similarity.

    Article Snippet: HPLC-FTMS The HPLC-FTMS system used an HPLC system (Accela LC with Accela Pump 60057-60010 and Accela Autosampler 60057-60020, Thermo Scientific, Dreieich, Germany) coupled to a Fourier transform mass spectrometer with a heated ESI source (LTQ Orbitrap XL, Thermo Scientific, Dreieich, Germany).

    Techniques: Expressing, Over Expression, Derivative Assay, Microarray, High Performance Liquid Chromatography, Mutagenesis