accela high speed liquid chromatography hplc  (Thermo Fisher)


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    Thermo Fisher accela high speed liquid chromatography hplc
    Accela High Speed Liquid Chromatography Hplc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accela high speed liquid chromatography hplc/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    accela high speed liquid chromatography hplc - by Bioz Stars, 2020-07
    90/100 stars

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    High Performance Liquid Chromatography:

    Article Title: Cissus sicyoides: Pharmacological Mechanisms Involved in the Anti-Inflammatory and Antidiarrheal Activities
    Article Snippet: .. Chemical Fingerprint of 70% EtOH by HPLC-PDA-ESI-IT-MS The fingerprint of HECS was obtained using an Accela High Speed liquid Chromatography (HPLC) (Thermo Cientific® , San José, CA, USA), Phenomenex® Luna C18 (250 mm × 4.6 mm i.d. .. ; 5 μm) column (Phenomenex Inc., Torrance, CA, USA), with a PAD detector and coupled to an Accela (Thermo Cientific® ) LCQ Fleet with Ion Trap 3D (IT) and ionization by electrospray (IES).

    Liquid Chromatography:

    Article Title: Cissus sicyoides: Pharmacological Mechanisms Involved in the Anti-Inflammatory and Antidiarrheal Activities
    Article Snippet: .. Chemical Fingerprint of 70% EtOH by HPLC-PDA-ESI-IT-MS The fingerprint of HECS was obtained using an Accela High Speed liquid Chromatography (HPLC) (Thermo Cientific® , San José, CA, USA), Phenomenex® Luna C18 (250 mm × 4.6 mm i.d. .. ; 5 μm) column (Phenomenex Inc., Torrance, CA, USA), with a PAD detector and coupled to an Accela (Thermo Cientific® ) LCQ Fleet with Ion Trap 3D (IT) and ionization by electrospray (IES).

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    Thermo Fisher hplc system
    Functional characterization of the PKS19 cluster, a putative polyketide biosynthetic gene cluster that is unique to F. fujikuroi . (A) Position and organization of the PKS19 gene cluster on F. fujikuroi chromosome VIII, GC content, distribution of active histone marks, and gene expression in the PKS19 cluster. Histone marks are described in the legend for Figure 3 . Expression data are from microarray analysis of wild-type F. fujikuroi (strain IMI58289). Values are log 2 change in expression in a high versus low-nitrogen medium. H3K9ac and gene expression are correlated, as both are increased in the high-nitrogen medium. Some genes exhibited increased levels of H3K4me2 in the high-nitrogen medium, which also suggests transcription. (B) Chemical analysis of the SM product(s) of the PKS19 cluster. The traces show the combined extracted ion chromatograms for metabolites with molecular formulas [C 12 H 16 O 4 +H] + , [C 12 H 18 O 5 +H] + and [C 12 H 18 O 4 +H] + determined by <t>HPLC-FTMS</t> of culture fluids from F. fujikuroi strains: IMI58289, wild-type strain; OE::TF, a strain over-expressing the transcription factor encoded by FFUJ_12242; OE::PKS19, a strain over-expressing the PKS19 gene FFUJ_12239; and OE::TF/OE::PKS19, a strain over-expressing both FFUJ-12242 and FFUJ_12239. (C) Northern blot analysis of PKS19 cluster genes in strains IMI58289 (WT), OE::TF, OE::PKS19 and OE::TF/OE::PKS19. (D) UV spectra of metabolites corresponding to peaks 1 through 4 from chromatograms shown in C. The similar spectra of the metabolites suggest structural similarity.
    Hplc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 587 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hplc system - by Bioz Stars, 2020-07
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    Thermo Fisher hplc dad system
    <t>HPLC-DAD</t> chromatograms of the methanol extracts (ME) from leaves (L) and stems (S) of Helicteres vegae (Hv) and Heliopsis sinaloensis (Hs). The identity of the major phenolics (flavonoids F and phenolic acids P) is shown in Table 1 . Peaks for the commercial standards are CA (caffeic acid) and R (rutin).
    Hplc Dad System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher hplc c18 system
    <t>HPLC</t> configurations. The fused-core <t>C18</t> and the C30 columns were used either individually alone (a) or connected in series so that the C18 column was followed; (b) directly by the C30 column then by the detector (C18–C30 system); (c) by one pressure resistant detector followed by the C30 column, then a second detector (C18-D-C30 system); (d) a 6-port valve which, in position I, directed the flow directly to the detector and, in position II, directed the flow to the C30 column and then to the detector (C18–C30dvs system).
    Hplc C18 System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Functional characterization of the PKS19 cluster, a putative polyketide biosynthetic gene cluster that is unique to F. fujikuroi . (A) Position and organization of the PKS19 gene cluster on F. fujikuroi chromosome VIII, GC content, distribution of active histone marks, and gene expression in the PKS19 cluster. Histone marks are described in the legend for Figure 3 . Expression data are from microarray analysis of wild-type F. fujikuroi (strain IMI58289). Values are log 2 change in expression in a high versus low-nitrogen medium. H3K9ac and gene expression are correlated, as both are increased in the high-nitrogen medium. Some genes exhibited increased levels of H3K4me2 in the high-nitrogen medium, which also suggests transcription. (B) Chemical analysis of the SM product(s) of the PKS19 cluster. The traces show the combined extracted ion chromatograms for metabolites with molecular formulas [C 12 H 16 O 4 +H] + , [C 12 H 18 O 5 +H] + and [C 12 H 18 O 4 +H] + determined by HPLC-FTMS of culture fluids from F. fujikuroi strains: IMI58289, wild-type strain; OE::TF, a strain over-expressing the transcription factor encoded by FFUJ_12242; OE::PKS19, a strain over-expressing the PKS19 gene FFUJ_12239; and OE::TF/OE::PKS19, a strain over-expressing both FFUJ-12242 and FFUJ_12239. (C) Northern blot analysis of PKS19 cluster genes in strains IMI58289 (WT), OE::TF, OE::PKS19 and OE::TF/OE::PKS19. (D) UV spectra of metabolites corresponding to peaks 1 through 4 from chromatograms shown in C. The similar spectra of the metabolites suggest structural similarity.

    Journal: PLoS Pathogens

    Article Title: Deciphering the Cryptic Genome: Genome-wide Analyses of the Rice Pathogen Fusarium fujikuroi Reveal Complex Regulation of Secondary Metabolism and Novel Metabolites

    doi: 10.1371/journal.ppat.1003475

    Figure Lengend Snippet: Functional characterization of the PKS19 cluster, a putative polyketide biosynthetic gene cluster that is unique to F. fujikuroi . (A) Position and organization of the PKS19 gene cluster on F. fujikuroi chromosome VIII, GC content, distribution of active histone marks, and gene expression in the PKS19 cluster. Histone marks are described in the legend for Figure 3 . Expression data are from microarray analysis of wild-type F. fujikuroi (strain IMI58289). Values are log 2 change in expression in a high versus low-nitrogen medium. H3K9ac and gene expression are correlated, as both are increased in the high-nitrogen medium. Some genes exhibited increased levels of H3K4me2 in the high-nitrogen medium, which also suggests transcription. (B) Chemical analysis of the SM product(s) of the PKS19 cluster. The traces show the combined extracted ion chromatograms for metabolites with molecular formulas [C 12 H 16 O 4 +H] + , [C 12 H 18 O 5 +H] + and [C 12 H 18 O 4 +H] + determined by HPLC-FTMS of culture fluids from F. fujikuroi strains: IMI58289, wild-type strain; OE::TF, a strain over-expressing the transcription factor encoded by FFUJ_12242; OE::PKS19, a strain over-expressing the PKS19 gene FFUJ_12239; and OE::TF/OE::PKS19, a strain over-expressing both FFUJ-12242 and FFUJ_12239. (C) Northern blot analysis of PKS19 cluster genes in strains IMI58289 (WT), OE::TF, OE::PKS19 and OE::TF/OE::PKS19. (D) UV spectra of metabolites corresponding to peaks 1 through 4 from chromatograms shown in C. The similar spectra of the metabolites suggest structural similarity.

    Article Snippet: HPLC-FTMS The HPLC-FTMS system used an HPLC system (Accela LC with Accela Pump 60057-60010 and Accela Autosampler 60057-60020, Thermo Scientific, Dreieich, Germany) coupled to a Fourier transform mass spectrometer with a heated ESI source (LTQ Orbitrap XL, Thermo Scientific, Dreieich, Germany).

    Techniques: Functional Assay, Expressing, Microarray, High Performance Liquid Chromatography, Northern Blot

    Location of the NRPS/APS biosynthetic gene cluster on F. fujikuroi chromosome I, levels of histone modifications and gene expression within and flanking the cluster, and production of metabolites following overexpression of cluster genes APS2 and APS8 . A : Synteny between the apicidin gene cluster in F. semitectum [105] and the apicidin-like gene cluster in F. fujikuroi . B : Histone modifications and gene expression in and flanking the cluster. Histone marks are described in the legend to Figure 3 . Expression data were derived from microarray experiments in low and high nitrogen and are plotted as the changes in log 2 expression values in high-nitrogen medium compared to low-nitrogen medium. H3K9ac and gene expression are overall correlated, as both are increased under high nitrogen conditions. In some genes increased H3K4me2 was observed, also suggesting transcription. C : Chemical analysis of the product of the unique PKS19 gene cluster. The traces show the extracted ion chromatograms for [C 34 H 48 O 6 N 5 +H] + (first line) and [C 35 H 42 O 7 N 5 +H] + (second to fourth line) determined by HPLC-FTMS of an apicidin standard (first line) and of culture fluids from F. fujikuroi IMI58289, OE::APS8 and the OE::APS2/OE::APS8 mutant. D : UV spectra of apicidin and the apicidin-like compound. The similar spectra suggest a structural similarity.

    Journal: PLoS Pathogens

    Article Title: Deciphering the Cryptic Genome: Genome-wide Analyses of the Rice Pathogen Fusarium fujikuroi Reveal Complex Regulation of Secondary Metabolism and Novel Metabolites

    doi: 10.1371/journal.ppat.1003475

    Figure Lengend Snippet: Location of the NRPS/APS biosynthetic gene cluster on F. fujikuroi chromosome I, levels of histone modifications and gene expression within and flanking the cluster, and production of metabolites following overexpression of cluster genes APS2 and APS8 . A : Synteny between the apicidin gene cluster in F. semitectum [105] and the apicidin-like gene cluster in F. fujikuroi . B : Histone modifications and gene expression in and flanking the cluster. Histone marks are described in the legend to Figure 3 . Expression data were derived from microarray experiments in low and high nitrogen and are plotted as the changes in log 2 expression values in high-nitrogen medium compared to low-nitrogen medium. H3K9ac and gene expression are overall correlated, as both are increased under high nitrogen conditions. In some genes increased H3K4me2 was observed, also suggesting transcription. C : Chemical analysis of the product of the unique PKS19 gene cluster. The traces show the extracted ion chromatograms for [C 34 H 48 O 6 N 5 +H] + (first line) and [C 35 H 42 O 7 N 5 +H] + (second to fourth line) determined by HPLC-FTMS of an apicidin standard (first line) and of culture fluids from F. fujikuroi IMI58289, OE::APS8 and the OE::APS2/OE::APS8 mutant. D : UV spectra of apicidin and the apicidin-like compound. The similar spectra suggest a structural similarity.

    Article Snippet: HPLC-FTMS The HPLC-FTMS system used an HPLC system (Accela LC with Accela Pump 60057-60010 and Accela Autosampler 60057-60020, Thermo Scientific, Dreieich, Germany) coupled to a Fourier transform mass spectrometer with a heated ESI source (LTQ Orbitrap XL, Thermo Scientific, Dreieich, Germany).

    Techniques: Expressing, Over Expression, Derivative Assay, Microarray, High Performance Liquid Chromatography, Mutagenesis

    HPLC-DAD chromatograms of the methanol extracts (ME) from leaves (L) and stems (S) of Helicteres vegae (Hv) and Heliopsis sinaloensis (Hs). The identity of the major phenolics (flavonoids F and phenolic acids P) is shown in Table 1 . Peaks for the commercial standards are CA (caffeic acid) and R (rutin).

    Journal: Pharmaceutical Biology

    Article Title: Chemical composition and biological activities of Helicteres vegae and Heliopsis sinaloensis

    doi: 10.1080/13880209.2017.1306712

    Figure Lengend Snippet: HPLC-DAD chromatograms of the methanol extracts (ME) from leaves (L) and stems (S) of Helicteres vegae (Hv) and Heliopsis sinaloensis (Hs). The identity of the major phenolics (flavonoids F and phenolic acids P) is shown in Table 1 . Peaks for the commercial standards are CA (caffeic acid) and R (rutin).

    Article Snippet: A 5 μL aliquot was injected into the HPLC-DAD system (ACCELA, Thermo Scientific, Waltham, MA).

    Techniques: High Performance Liquid Chromatography

    HPLC configurations. The fused-core C18 and the C30 columns were used either individually alone (a) or connected in series so that the C18 column was followed; (b) directly by the C30 column then by the detector (C18–C30 system); (c) by one pressure resistant detector followed by the C30 column, then a second detector (C18-D-C30 system); (d) a 6-port valve which, in position I, directed the flow directly to the detector and, in position II, directed the flow to the C30 column and then to the detector (C18–C30dvs system).

    Journal: Journal of chromatography. A

    Article Title: Simultaneous analysis of circulating 25-hydroxy-vitamin D3, 25-hydroxy-vitamin D2, retinol, tocopherols, carotenoids, and oxidized and reduced coenzyme Q10 by high performance liquid chromatography with photo diode-array detection using C18 and C30 columns alone or in combination

    doi: 10.1016/j.chroma.2013.05.027

    Figure Lengend Snippet: HPLC configurations. The fused-core C18 and the C30 columns were used either individually alone (a) or connected in series so that the C18 column was followed; (b) directly by the C30 column then by the detector (C18–C30 system); (c) by one pressure resistant detector followed by the C30 column, then a second detector (C18-D-C30 system); (d) a 6-port valve which, in position I, directed the flow directly to the detector and, in position II, directed the flow to the C30 column and then to the detector (C18–C30dvs system).

    Article Snippet: An HPLC C18 system (Model Accela, ThermoFisher, San Jose, CA, USA) monitored the reaction progress, which was deemed complete when all starting material disappeared (UN10: RT = 6.1 min) and the new product peaks emerged (Et-UN10: RT = 8.3 min; Bu-UN10: RT = 13.1 min).

    Techniques: High Performance Liquid Chromatography, Flow Cytometry