accela 600 pump  (Thermo Fisher)


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    Structured Review

    Thermo Fisher accela 600 pump
    Lipid profiling of the optic nerve (ON) regeneration . ( a ) The dataset consists of the following experimental groups: intact control, ON crush (followed by intravitreal injection of the vehicle) and inflammation-induced ON regeneration (ON crush followed by intravitreal injection of Zymosan + CPT-cAMP). ONs were harvested 3, 7, 14 days post-crush and retinas 7, 14 days post-crush. ( b ) In the longitudinal sections of rat ON, Zymosan + CPT-cAMP increases expression of a marker of axon regeneration, GAP43, distal to the crush site (*). ( c ) Following methanol-chloroform-based extraction, lipids were separated by C30 high-performance liquid chromatography (HPLC) system using <t>Accela</t> 600 pump and measured in (+)/(−) heated electrospray (HESI) ionization mode using a Q Exactive mass spectrometer. Lipidome identification and relative quantification were performed in LipidSearch, followed by statistical analysis in MetaboAnalyst.
    Accela 600 Pump, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accela 600 pump/product/Thermo Fisher
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    accela 600 pump - by Bioz Stars, 2020-07
    88/100 stars

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    1) Product Images from "Comparative lipid profiling dataset of the inflammation-induced optic nerve regeneration"

    Article Title: Comparative lipid profiling dataset of the inflammation-induced optic nerve regeneration

    Journal: Data in Brief

    doi: 10.1016/j.dib.2019.103950

    Lipid profiling of the optic nerve (ON) regeneration . ( a ) The dataset consists of the following experimental groups: intact control, ON crush (followed by intravitreal injection of the vehicle) and inflammation-induced ON regeneration (ON crush followed by intravitreal injection of Zymosan + CPT-cAMP). ONs were harvested 3, 7, 14 days post-crush and retinas 7, 14 days post-crush. ( b ) In the longitudinal sections of rat ON, Zymosan + CPT-cAMP increases expression of a marker of axon regeneration, GAP43, distal to the crush site (*). ( c ) Following methanol-chloroform-based extraction, lipids were separated by C30 high-performance liquid chromatography (HPLC) system using Accela 600 pump and measured in (+)/(−) heated electrospray (HESI) ionization mode using a Q Exactive mass spectrometer. Lipidome identification and relative quantification were performed in LipidSearch, followed by statistical analysis in MetaboAnalyst.
    Figure Legend Snippet: Lipid profiling of the optic nerve (ON) regeneration . ( a ) The dataset consists of the following experimental groups: intact control, ON crush (followed by intravitreal injection of the vehicle) and inflammation-induced ON regeneration (ON crush followed by intravitreal injection of Zymosan + CPT-cAMP). ONs were harvested 3, 7, 14 days post-crush and retinas 7, 14 days post-crush. ( b ) In the longitudinal sections of rat ON, Zymosan + CPT-cAMP increases expression of a marker of axon regeneration, GAP43, distal to the crush site (*). ( c ) Following methanol-chloroform-based extraction, lipids were separated by C30 high-performance liquid chromatography (HPLC) system using Accela 600 pump and measured in (+)/(−) heated electrospray (HESI) ionization mode using a Q Exactive mass spectrometer. Lipidome identification and relative quantification were performed in LipidSearch, followed by statistical analysis in MetaboAnalyst.

    Techniques Used: Injection, Cycling Probe Technology, Expressing, Marker, High Performance Liquid Chromatography, Mass Spectrometry

    Related Articles

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Metabolic and Lipidomic Profiling of Vegetable Juices Fermented with Various Probiotics
    Article Snippet: .. LC-MS analysis of each sample was conducted as previously reported, using an Accela LC (Thermo Fisher Scientific, San Jose, CA, USA) equipped with a degasser, Accela 600 pump, linear ion-trap mass spectrometer (LTQ-XL, Thermo Fisher Scientific), and Accela AS autosampler [ ]. .. A 1.9 µm Hypersil Gold column (Part no. 25002-102130; 100 mm × 2.1 mm; Thermo Scientific, San Jose, CA, USA) was used, and the column oven temperature was 35 °C.

    Article Title: Serine starvation induces stress and p53 dependent metabolic remodeling in cancer cells
    Article Snippet: .. LC-MS analyses were performed on an Orbitrap Exactive (Thermo Scientific) in line with an Accela autosampler and an Accela 600 pump (Thermo Scientific). ..

    Chromatography:

    Article Title: Identification of midgut membrane proteins from different instars of Helicoverpa armigera (Lepidoptera: Noctuidae) that bind to Cry1Ac toxin
    Article Snippet: .. The sliced gel samples were reduced with dithiothreitol (DTT) (Sigma-Aldrich; St Louis, MO, USA) at 56°C for 30 min. Then, samples were alkylated with iodoacetamide (Sigma-Aldrich; St Louis, MO, USA) for 30 min in dark conditions and treated with Trypsin (Promega Sequencing Grade Modified Trypsin, Madison, WI, USA) (1: 25) at pH 8.1 at 37°C for 12 h. The peptides produced by the enzymatic cleavage were desalted with Zip Tip C18 (Millipore; Billerica, MA, USA) and five μg applied in an LC-MS/MS (Liquid Chromatography-Mass Spectrometry) system composed of an accela 600 pump (Thermo-Fisher Co.; San Jose, CA, USA) coupled to a mass spectrometer LTQ-Orbitrap Velos (Thermo-Fisher Co., San Jose, CA, USA) with nano-electrospray (ESI) ionization source. ..

    Produced:

    Article Title: Identification of midgut membrane proteins from different instars of Helicoverpa armigera (Lepidoptera: Noctuidae) that bind to Cry1Ac toxin
    Article Snippet: .. The sliced gel samples were reduced with dithiothreitol (DTT) (Sigma-Aldrich; St Louis, MO, USA) at 56°C for 30 min. Then, samples were alkylated with iodoacetamide (Sigma-Aldrich; St Louis, MO, USA) for 30 min in dark conditions and treated with Trypsin (Promega Sequencing Grade Modified Trypsin, Madison, WI, USA) (1: 25) at pH 8.1 at 37°C for 12 h. The peptides produced by the enzymatic cleavage were desalted with Zip Tip C18 (Millipore; Billerica, MA, USA) and five μg applied in an LC-MS/MS (Liquid Chromatography-Mass Spectrometry) system composed of an accela 600 pump (Thermo-Fisher Co.; San Jose, CA, USA) coupled to a mass spectrometer LTQ-Orbitrap Velos (Thermo-Fisher Co., San Jose, CA, USA) with nano-electrospray (ESI) ionization source. ..

    Sequencing:

    Article Title: Identification of midgut membrane proteins from different instars of Helicoverpa armigera (Lepidoptera: Noctuidae) that bind to Cry1Ac toxin
    Article Snippet: .. The sliced gel samples were reduced with dithiothreitol (DTT) (Sigma-Aldrich; St Louis, MO, USA) at 56°C for 30 min. Then, samples were alkylated with iodoacetamide (Sigma-Aldrich; St Louis, MO, USA) for 30 min in dark conditions and treated with Trypsin (Promega Sequencing Grade Modified Trypsin, Madison, WI, USA) (1: 25) at pH 8.1 at 37°C for 12 h. The peptides produced by the enzymatic cleavage were desalted with Zip Tip C18 (Millipore; Billerica, MA, USA) and five μg applied in an LC-MS/MS (Liquid Chromatography-Mass Spectrometry) system composed of an accela 600 pump (Thermo-Fisher Co.; San Jose, CA, USA) coupled to a mass spectrometer LTQ-Orbitrap Velos (Thermo-Fisher Co., San Jose, CA, USA) with nano-electrospray (ESI) ionization source. ..

    Mass Spectrometry:

    Article Title: Metabolic and Lipidomic Profiling of Vegetable Juices Fermented with Various Probiotics
    Article Snippet: .. LC-MS analysis of each sample was conducted as previously reported, using an Accela LC (Thermo Fisher Scientific, San Jose, CA, USA) equipped with a degasser, Accela 600 pump, linear ion-trap mass spectrometer (LTQ-XL, Thermo Fisher Scientific), and Accela AS autosampler [ ]. .. A 1.9 µm Hypersil Gold column (Part no. 25002-102130; 100 mm × 2.1 mm; Thermo Scientific, San Jose, CA, USA) was used, and the column oven temperature was 35 °C.

    Article Title: Identification of midgut membrane proteins from different instars of Helicoverpa armigera (Lepidoptera: Noctuidae) that bind to Cry1Ac toxin
    Article Snippet: .. The sliced gel samples were reduced with dithiothreitol (DTT) (Sigma-Aldrich; St Louis, MO, USA) at 56°C for 30 min. Then, samples were alkylated with iodoacetamide (Sigma-Aldrich; St Louis, MO, USA) for 30 min in dark conditions and treated with Trypsin (Promega Sequencing Grade Modified Trypsin, Madison, WI, USA) (1: 25) at pH 8.1 at 37°C for 12 h. The peptides produced by the enzymatic cleavage were desalted with Zip Tip C18 (Millipore; Billerica, MA, USA) and five μg applied in an LC-MS/MS (Liquid Chromatography-Mass Spectrometry) system composed of an accela 600 pump (Thermo-Fisher Co.; San Jose, CA, USA) coupled to a mass spectrometer LTQ-Orbitrap Velos (Thermo-Fisher Co., San Jose, CA, USA) with nano-electrospray (ESI) ionization source. ..

    Modification:

    Article Title: Identification of midgut membrane proteins from different instars of Helicoverpa armigera (Lepidoptera: Noctuidae) that bind to Cry1Ac toxin
    Article Snippet: .. The sliced gel samples were reduced with dithiothreitol (DTT) (Sigma-Aldrich; St Louis, MO, USA) at 56°C for 30 min. Then, samples were alkylated with iodoacetamide (Sigma-Aldrich; St Louis, MO, USA) for 30 min in dark conditions and treated with Trypsin (Promega Sequencing Grade Modified Trypsin, Madison, WI, USA) (1: 25) at pH 8.1 at 37°C for 12 h. The peptides produced by the enzymatic cleavage were desalted with Zip Tip C18 (Millipore; Billerica, MA, USA) and five μg applied in an LC-MS/MS (Liquid Chromatography-Mass Spectrometry) system composed of an accela 600 pump (Thermo-Fisher Co.; San Jose, CA, USA) coupled to a mass spectrometer LTQ-Orbitrap Velos (Thermo-Fisher Co., San Jose, CA, USA) with nano-electrospray (ESI) ionization source. ..

    Liquid Chromatography:

    Article Title: Chemometric Characterization of Strawberries and Blueberries according to Their Phenolic Profile: Combined Effect of Cultivar and Cultivation System
    Article Snippet: .. The separation of compounds was performed while using ultra-high performance liquid chromatography (UHPLC) system that consisted of an Accela 600 pump and an Accela auto-sampler (Thermo Fisher Scientific, Waltham, MA, USA). .. The column used for analytical separation was a Syncronis C18 column (50 × 2.1 mm, 1.7 μm particle size, Thermo Fisher Scientific).

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  • 88
    Thermo Fisher accela 600 pump
    Lipid profiling of the optic nerve (ON) regeneration . ( a ) The dataset consists of the following experimental groups: intact control, ON crush (followed by intravitreal injection of the vehicle) and inflammation-induced ON regeneration (ON crush followed by intravitreal injection of Zymosan + CPT-cAMP). ONs were harvested 3, 7, 14 days post-crush and retinas 7, 14 days post-crush. ( b ) In the longitudinal sections of rat ON, Zymosan + CPT-cAMP increases expression of a marker of axon regeneration, GAP43, distal to the crush site (*). ( c ) Following methanol-chloroform-based extraction, lipids were separated by C30 high-performance liquid chromatography (HPLC) system using <t>Accela</t> 600 pump and measured in (+)/(−) heated electrospray (HESI) ionization mode using a Q Exactive mass spectrometer. Lipidome identification and relative quantification were performed in LipidSearch, followed by statistical analysis in MetaboAnalyst.
    Accela 600 Pump, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accela 600 pump/product/Thermo Fisher
    Average 88 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    accela 600 pump - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    86
    Thermo Fisher quaternary accela 600 pump
    Lipid profiling of the optic nerve (ON) regeneration . ( a ) The dataset consists of the following experimental groups: intact control, ON crush (followed by intravitreal injection of the vehicle) and inflammation-induced ON regeneration (ON crush followed by intravitreal injection of Zymosan + CPT-cAMP). ONs were harvested 3, 7, 14 days post-crush and retinas 7, 14 days post-crush. ( b ) In the longitudinal sections of rat ON, Zymosan + CPT-cAMP increases expression of a marker of axon regeneration, GAP43, distal to the crush site (*). ( c ) Following methanol-chloroform-based extraction, lipids were separated by C30 high-performance liquid chromatography (HPLC) system using <t>Accela</t> 600 pump and measured in (+)/(−) heated electrospray (HESI) ionization mode using a Q Exactive mass spectrometer. Lipidome identification and relative quantification were performed in LipidSearch, followed by statistical analysis in MetaboAnalyst.
    Quaternary Accela 600 Pump, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quaternary accela 600 pump/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quaternary accela 600 pump - by Bioz Stars, 2020-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    Lipid profiling of the optic nerve (ON) regeneration . ( a ) The dataset consists of the following experimental groups: intact control, ON crush (followed by intravitreal injection of the vehicle) and inflammation-induced ON regeneration (ON crush followed by intravitreal injection of Zymosan + CPT-cAMP). ONs were harvested 3, 7, 14 days post-crush and retinas 7, 14 days post-crush. ( b ) In the longitudinal sections of rat ON, Zymosan + CPT-cAMP increases expression of a marker of axon regeneration, GAP43, distal to the crush site (*). ( c ) Following methanol-chloroform-based extraction, lipids were separated by C30 high-performance liquid chromatography (HPLC) system using Accela 600 pump and measured in (+)/(−) heated electrospray (HESI) ionization mode using a Q Exactive mass spectrometer. Lipidome identification and relative quantification were performed in LipidSearch, followed by statistical analysis in MetaboAnalyst.

    Journal: Data in Brief

    Article Title: Comparative lipid profiling dataset of the inflammation-induced optic nerve regeneration

    doi: 10.1016/j.dib.2019.103950

    Figure Lengend Snippet: Lipid profiling of the optic nerve (ON) regeneration . ( a ) The dataset consists of the following experimental groups: intact control, ON crush (followed by intravitreal injection of the vehicle) and inflammation-induced ON regeneration (ON crush followed by intravitreal injection of Zymosan + CPT-cAMP). ONs were harvested 3, 7, 14 days post-crush and retinas 7, 14 days post-crush. ( b ) In the longitudinal sections of rat ON, Zymosan + CPT-cAMP increases expression of a marker of axon regeneration, GAP43, distal to the crush site (*). ( c ) Following methanol-chloroform-based extraction, lipids were separated by C30 high-performance liquid chromatography (HPLC) system using Accela 600 pump and measured in (+)/(−) heated electrospray (HESI) ionization mode using a Q Exactive mass spectrometer. Lipidome identification and relative quantification were performed in LipidSearch, followed by statistical analysis in MetaboAnalyst.

    Article Snippet: Reversed-phase chromatographic separation was achieved with the Accela Autosampler, Accela 600 pump and Acclaim C30 column: 3 μm, 2.1 × 150 mm (Thermo Fisher Scientific, Waltham, MA).

    Techniques: Injection, Cycling Probe Technology, Expressing, Marker, High Performance Liquid Chromatography, Mass Spectrometry