acc65i  (New England Biolabs)


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    New England Biolabs acc65i
    Acc65i, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acc65i/product/New England Biolabs
    Average 94 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    acc65i - by Bioz Stars, 2022-08
    94/100 stars

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    New England Biolabs acc65i digested
    Complementation of B101 mutant with Rv2983 . A. Schematic diagram of genomic DNA of M. tuberculosis strains after digestion with restriction enzyme <t>Acc65I;</t> B. Result of southern blot confirmed expected DNA fragments after Acc65I digestion using DIG-labeled Rv2983 probe (H37Rv: 6.3 kb; Rv2983 mutant: 6.3 kb; complemented strains: 6.3 and 3.5 kb).
    Acc65i Digested, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acc65i digested/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    acc65i digested - by Bioz Stars, 2022-08
    94/100 stars
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    Complementation of B101 mutant with Rv2983 . A. Schematic diagram of genomic DNA of M. tuberculosis strains after digestion with restriction enzyme Acc65I; B. Result of southern blot confirmed expected DNA fragments after Acc65I digestion using DIG-labeled Rv2983 probe (H37Rv: 6.3 kb; Rv2983 mutant: 6.3 kb; complemented strains: 6.3 and 3.5 kb).

    Journal: bioRxiv

    Article Title: Mutations in Rv2983 as a novel determinant of resistance to nitroimidazole drugs in Mycobacterium tuberculosis

    doi: 10.1101/457754

    Figure Lengend Snippet: Complementation of B101 mutant with Rv2983 . A. Schematic diagram of genomic DNA of M. tuberculosis strains after digestion with restriction enzyme Acc65I; B. Result of southern blot confirmed expected DNA fragments after Acc65I digestion using DIG-labeled Rv2983 probe (H37Rv: 6.3 kb; Rv2983 mutant: 6.3 kb; complemented strains: 6.3 and 3.5 kb).

    Article Snippet: Acc65I-digested (NE biolabs) genomic DNA of the wild type, the B101 mutant and the B101pRv2983 and B101phsp60 -Rv2983 complemented strains was separated on agarose gel and transferred onto positively-charged nylon-membrane (GE).

    Techniques: Mutagenesis, Southern Blot, Labeling