Structured Review

PerkinElmer ac coa
Ac Coa, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 81/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ac coa/product/PerkinElmer
Average 81 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ac coa - by Bioz Stars, 2019-10
81/100 stars

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In Vitro:

Article Title: Activity of acetyltransferase toxins involved in Salmonella persister formation during macrophage infection
Article Snippet: Refolded TacT1–3 was concentrated and stored at −80 °C. .. In vitro cell-free expression assays were carried out using the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs [NEB], Massachusetts, USA), following the manufacturer’s guidelines, with the exception of the addition of purified toxins (2 ng) supplemented or not with Ac-CoA (2 mM—Sigma-Aldrich) or 14 C Ac-CoA (0.2 mM, 3.7 mBq—Perkin Elmer, Massachusetts, USA) where specified. .. Samples were analysed by SDS-PAGE or by acid/urea PAGE and visualized by Coomassie Blue staining or by autoradiography.

Expressing:

Article Title: Activity of acetyltransferase toxins involved in Salmonella persister formation during macrophage infection
Article Snippet: Refolded TacT1–3 was concentrated and stored at −80 °C. .. In vitro cell-free expression assays were carried out using the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs [NEB], Massachusetts, USA), following the manufacturer’s guidelines, with the exception of the addition of purified toxins (2 ng) supplemented or not with Ac-CoA (2 mM—Sigma-Aldrich) or 14 C Ac-CoA (0.2 mM, 3.7 mBq—Perkin Elmer, Massachusetts, USA) where specified. .. Samples were analysed by SDS-PAGE or by acid/urea PAGE and visualized by Coomassie Blue staining or by autoradiography.

Purification:

Article Title: Activity of acetyltransferase toxins involved in Salmonella persister formation during macrophage infection
Article Snippet: Refolded TacT1–3 was concentrated and stored at −80 °C. .. In vitro cell-free expression assays were carried out using the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs [NEB], Massachusetts, USA), following the manufacturer’s guidelines, with the exception of the addition of purified toxins (2 ng) supplemented or not with Ac-CoA (2 mM—Sigma-Aldrich) or 14 C Ac-CoA (0.2 mM, 3.7 mBq—Perkin Elmer, Massachusetts, USA) where specified. .. Samples were analysed by SDS-PAGE or by acid/urea PAGE and visualized by Coomassie Blue staining or by autoradiography.

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    PerkinElmer h ac coa
    Linear correlation of the SPA with the filter binding assay. A time-dependent assay was carried out using identical conditions for both assays. Samples containing 2.5 μM <t>H3(1–20)-BTN,</t> 1 μM [ 3 <t>H]Ac-CoA,</t> and 0.025 μM
    H Ac Coa, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h ac coa/product/PerkinElmer
    Average 77 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    h ac coa - by Bioz Stars, 2019-10
    77/100 stars
      Buy from Supplier

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    Linear correlation of the SPA with the filter binding assay. A time-dependent assay was carried out using identical conditions for both assays. Samples containing 2.5 μM H3(1–20)-BTN, 1 μM [ 3 H]Ac-CoA, and 0.025 μM

    Journal:

    Article Title: Effective Quenchers Are Required to Eliminate the Interference of Substrate: Cofactor Binding in the HAT Scintillation Proximity Assay

    doi: 10.1089/adt.2015.636

    Figure Lengend Snippet: Linear correlation of the SPA with the filter binding assay. A time-dependent assay was carried out using identical conditions for both assays. Samples containing 2.5 μM H3(1–20)-BTN, 1 μM [ 3 H]Ac-CoA, and 0.025 μM

    Article Snippet: The cofactor used as an acetyl donor was [3 H]Ac-CoA (PerkinElmer), and the substrate was either H4(1–20)-BTN or H3(1–20)-BTN.

    Techniques: Filter-binding Assay

    Interaction of histone H3 and H4 substrates with [ 3 H]Ac-CoA measured by SPA (A) and the effects of various quenchers on the interaction between substrate and cofactor (B) . Assays containing peptide [0–10 μM H3(1–20)-BTN

    Journal:

    Article Title: Effective Quenchers Are Required to Eliminate the Interference of Substrate: Cofactor Binding in the HAT Scintillation Proximity Assay

    doi: 10.1089/adt.2015.636

    Figure Lengend Snippet: Interaction of histone H3 and H4 substrates with [ 3 H]Ac-CoA measured by SPA (A) and the effects of various quenchers on the interaction between substrate and cofactor (B) . Assays containing peptide [0–10 μM H3(1–20)-BTN

    Article Snippet: The cofactor used as an acetyl donor was [3 H]Ac-CoA (PerkinElmer), and the substrate was either H4(1–20)-BTN or H3(1–20)-BTN.

    Techniques:

    Quenching effects of 1xRB, isopropanol, DMSO, NaHCO 3, and guanidine HCl on samples (A) without HAT enzyme, and (B) with p300 enzyme. (A) Samples containing 2.5 μM H3(1–20)-BTN, and 1 μM [ 3 H]Ac-CoA was incubated

    Journal:

    Article Title: Effective Quenchers Are Required to Eliminate the Interference of Substrate: Cofactor Binding in the HAT Scintillation Proximity Assay

    doi: 10.1089/adt.2015.636

    Figure Lengend Snippet: Quenching effects of 1xRB, isopropanol, DMSO, NaHCO 3, and guanidine HCl on samples (A) without HAT enzyme, and (B) with p300 enzyme. (A) Samples containing 2.5 μM H3(1–20)-BTN, and 1 μM [ 3 H]Ac-CoA was incubated

    Article Snippet: The cofactor used as an acetyl donor was [3 H]Ac-CoA (PerkinElmer), and the substrate was either H4(1–20)-BTN or H3(1–20)-BTN.

    Techniques: HAT Assay, Incubation

    Dose-dependent inhibition of p300 activity by C646 measured using SPA and filter binding assay. Both the SPA and filter binding assays included 0.025 μM p300, 2.5 μM H3(1–20)-BTN, 1 μM [ 3 H]Ac-CoA,

    Journal:

    Article Title: Effective Quenchers Are Required to Eliminate the Interference of Substrate: Cofactor Binding in the HAT Scintillation Proximity Assay

    doi: 10.1089/adt.2015.636

    Figure Lengend Snippet: Dose-dependent inhibition of p300 activity by C646 measured using SPA and filter binding assay. Both the SPA and filter binding assays included 0.025 μM p300, 2.5 μM H3(1–20)-BTN, 1 μM [ 3 H]Ac-CoA,

    Article Snippet: The cofactor used as an acetyl donor was [3 H]Ac-CoA (PerkinElmer), and the substrate was either H4(1–20)-BTN or H3(1–20)-BTN.

    Techniques: Inhibition, Activity Assay, Filter-binding Assay, Binding Assay