Journal: Animal Nutrition

Article Title: PPARα-CD36-CAV1-mediated docosahexaenoic acid (DHA) uptake promotes muscle fiber development in grass carp ( Ctenopharyngodon idellus )

doi: 10.1016/j.aninu.2025.10.011

Figure Lengend Snippet: Docosahexaenoic acid (DHA) enhances the proliferation of grass carp myoblasts through the cluster of differentiation 36 (CD36)-mediated uptake mechanism. (A and B) The relative mRNA expression levels of proliferation-related genes (cyclin D1 and cyclin E) in myoblasts treated with DHA for 24 h. (C) Percentage of 5-ethynyl-2′-deoxyuridine (EdU)-positive myoblastsrelative to the total myoblasts. (D) EdU (red fluorescence) and Hoechst (blue fluorescence, nuclei) staining. Scale bar, 200 μm. Control, untreated; DHA, treated with 50 μmol/L DHA; DHA + SSO, co-treated with 50 μmol/L DHA and 200 μmol/L SSO. SSO = sulfosuccinimidyl oleate sodium (a CD36 inhibitor). P -value less than 0.05 indicates a significant difference, n = 3.

Article Snippet: To investigate whether DHA promotes myoblast proliferation via the CD36-mediated absorption pathway, this study utilized DHA (HY-B2167) and the CD36 inhibitor sulfosuccinimidyl oleate sodium (SSO; HY-112847A), both from MedChem Express LLC (Monmouth Junction, NJ, USA).

Techniques: Expressing, Fluorescence, Staining, Control

Journal: Animal Nutrition

Article Title: PPARα-CD36-CAV1-mediated docosahexaenoic acid (DHA) uptake promotes muscle fiber development in grass carp ( Ctenopharyngodon idellus )

doi: 10.1016/j.aninu.2025.10.011

Figure Lengend Snippet: Docosahexaenoic acid (DHA) promotes myofiber proliferation and development in muscle tissue via the PPARα-cluster of differentiation 36 (CD36)-mediated uptake pathway. (A) Site-directed mutagenesis analysis of PPARα binding sites on the pGL3.0-CD36 vector in human embryonic kidney 293T (HEK 293T) cells. (B) The mRNA expression levels of fatty acid absorption ( cd36 and cav1 ) in muscle tissue. (C) Muscle fiber diameter. (D) Muscle fiber density. (E) Representative hematoxylin and eosin (H&E) staining in muscle tissue. Scale bar, 100 μm. (F) The mRNA expression of muscle growth and development related genes ( myog , myod , myhc , mrf4 , and myf5 ) and fiber cell growth factor ( fgf6a and fgf6b ) in muscle tissue. Control, diet without DHA supplementation; DHA, diet supplemented with 0.5% DHA; DHA + GW6471, diet supplemented with 0.5% DHA and GW6471. GW6471 = peroxisome proliferator-activated receptor α inhibitor; Luc = luciferase. P -value less than 0.05 indicates a significant difference, n = 3.

Article Snippet: To investigate whether DHA promotes myoblast proliferation via the CD36-mediated absorption pathway, this study utilized DHA (HY-B2167) and the CD36 inhibitor sulfosuccinimidyl oleate sodium (SSO; HY-112847A), both from MedChem Express LLC (Monmouth Junction, NJ, USA).

Techniques: Mutagenesis, Binding Assay, Plasmid Preparation, Expressing, Staining, Control, Luciferase

Journal: Materials Today Bio

Article Title: Dual activation of cuproptosis and excessive autophagy by copper-bismuth metal-organic frameworks-loaded detachable microneedles in oral leukoplakia

doi: 10.1016/j.mtbio.2026.103283

Figure Lengend Snippet: Synthesis and Characterizations of CBM. (A) Schematic diagram of CBM synthesis. (B) Representative TEM image of CBM. (C) EDS analysis of CBM to determine elemental composition and distribution. (D, E) XPS characterization of CBM revealing the valence states of Cu and Bi. (F, G) Time-dependent depletion of GSH triggered by CBM, as determined by DTNB-based absorbance measurements at 420 nm. The p -values were analyzed by one-way ANOVA. # p < 0.05, ### p < 0.001. (H) UV-vis absorbance at 350 nm was measured after 1 h co-incubation of CBM with GSH to evaluate the formation of Bi(GS) 3 complexes.

Article Snippet: Ultraviolet-visible (UV-Vis) absorption spectra were recorded using a UV-8000 spectrophotometer (Shanghai Metash Instruments Co., Ltd., China) to assess the optical properties of the samples.

Techniques: Incubation