abi quantstudio 6 flex system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher abi quantstudio 6 flex system
    Abi Quantstudio 6 Flex System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi quantstudio 6 flex system/product/Thermo Fisher
    Average 90 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    abi quantstudio 6 flex system - by Bioz Stars, 2020-03
    90/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: RNA–DNA hybrids promote the expansion of Friedreich's ataxia (GAA)n repeats via break-induced replication
    Article Snippet: .. Following the RT reaction, samples were treated with 0.5U RNase H at 37°C for 20 min. Quantitative PCR (qPCR) was performed in 10 μl volumes using SYBR Select Master Mix (ThermoFisher) on a QuantStudio 6 Flex RTPCR system (Applied Biosystems) in 384-well plates with four technical replicates per sample. ..

    Article Title: Bread Wheat With High Salinity and Sodicity Tolerance
    Article Snippet: .. Amplification were performed in a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher) with 3 min of 95°C followed by 40 cycles of 3s at 95°C, 20s at 60°C, and fluorescent acquisition at 60°C, followed by melt curve analysis. .. Three wheat genes, encoding actin, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and elongation factor 1-alpha (EF1a) were used together for normalization of target gene expression.

    Article Title: Antidepressant Effect of Fraxinus rhynchophylla Hance Extract in a Mouse Model of Chronic Stress-Induced Depression
    Article Snippet: .. Real-time PCR primers were loaded onto a MicroAmp Fast 96-well reaction plate (Applied Biosystems) with Power SYBR Green PCR Master Mix (Applied Biosystems) and mRNA was quantified using Quantstudio 6 Flex (Applied Biosystems by Life Technologies). .. The sequences of the real-time PCR primers were as follows: 5′-CTTGGCAACAGCACAGACC-3′ (forward), and 5′-GAGAAGTCCATGTCCGCAAT-3′ (reverse) for NF-κB p65 ; 5′- AGACCCTCACACTCAGAT CATCTTC-3′ (forward) and 5′-CCACTTGGTGGTTTGCTACGA-3′ (reverse) for TNFα ; 5′-GAGGATACCACTCCCAACAGACC-3′ (forward) and 5′-AAGTGCATCATCGTTGTTCATACA-3′ (reverse) for IL-6 ; 5′-AGACATGGAGTCATAGGCTCTG-3′ (forward) and 5′-CCATTTTCCTTCTT GTG GAGCA-3′ (reverse) for IL-12 ; and 5′-AAGGTGGTGAAGCAGGCA T-3′ (forward) and 5′-GGTCCAGGGTTTCTTACTCCT-3′ (reverse) for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) .

    Article Title: MicroRNA Expression Profiling in Psoriatic Arthritis
    Article Snippet: .. Real-time PCR was carried out in triplicate on a QuantStudio 6 Flex instrument (Applied Biosystems, Foster City, CA, USA). .. Expression values were obtained by ΔCt method using QuantStudio Real-Time PCR system software v. 1.3.

    Article Title: A novel HRM assay for the simultaneous detection and differentiation of eight poxviruses of medical and veterinary importance
    Article Snippet: .. Thus, using the same PCR mix and protocol as in the BioRad CFX96 instrument, PCR and melting curve acquisition analysis was also conducted on the LightCycler® 480 Real-Time PCR Systems (Roche), QuantStudio™ 6 Flex Real-Time PCR System (Life Technologies), and Rotor-Gene Q Real-Time PCR Cycler (Qiagen). ..

    Article Title: Exposure–Response Associations of Household Air Pollution and Buccal Cell Telomere Length in Women Using Biomass Stoves
    Article Snippet: .. The thermal cycling conditions were 95°C for 30 s followed by 40 cycles of 95°C for 5 s, 54°C for 30 s, and 72°C for 31 s. PCR reactions were conducted on a QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems™). ..

    Article Title: The Rosa genome provides new insights into the domestication of modern roses
    Article Snippet: .. 5.8S rRNA and miR156 expression were quantified on QuantStudio™ 6 Flex Real-Time PCR 384 (Applied Biosystems) using Fast SYBR® Green Master Mix kit (Roche Diagnostic) and specific primers ( ). ..

    Article Title: Exon Skipping in the RET Gene Encodes Novel Isoforms That Differentially Regulate RET Protein Signal Transduction *
    Article Snippet: .. RT-PCR reactions with amplicons greater than 200 bp were performed using the Phusion high-fidelity DNA polymerase kit (New England Biolabs) following the instructions of the manufacturer. qPCR was performed using FastStart Universal SYBR Green Master Mix (Roche) and run on a QuantStudio 6 Flex real-time PCR system (Thermo Fisher Scientific). .. Quantitative analyses were performed by calculating the ΔCt (Ct of transcript divided by Ct of the housekeeping gene actin ) for each gene analyzed and transforming that value to log2 .

    Article Title: TRF2 positively regulates SULF2 expression increasing VEGF-A release and activity in tumor microenvironment
    Article Snippet: .. Real-time rt -PCRs were performed on the obtained cDNAs by using Fast power Syber green master mix (Apply Biosystem) on either QuantStudio 6-Flex (Thermo Fisher Scientific) or 7900HT Fast Real-Time PCR System (Applied Biosystem) thermocycler. .. For each sample 5 μl of the 1:10 diluted cDNA was mixed with 0.5 μl of each primer (10 μM), 10 μl of the sybr green master mix and water at final volume of 20 μl.

    Article Title: Constriction of Retinal Venules to Endothelin-1: Obligatory Roles of ETA Receptors, Extracellular Calcium Entry, and Rho Kinase
    Article Snippet: .. Real-time PCR was performed by monitoring the increase in SYBR Green fluorescence using the Applied Biosystems QuantStudio 6 Flex Real-Time PCR System (Life Technologies Corporation, Carlsbad, CA, USA). ..

    Article Title: The targetable kinase PIM1 drives ALK inhibitor resistance in high-risk neuroblastoma independent of MYCN status
    Article Snippet: .. In total, 1 µg RNA was reverse transcribed using iScript Reverse Transcription Supermix (Bio-Rad, Cat#1708840) and 20 ng cDNA template was used for qPCR. cDNA was amplified with PowerUp SYBR Green Master Mix (Thermo Fisher Scientific, Cat#A25918) and 600 nM primers on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) under standard cycling conditions. ..

    RNA Extraction:

    Article Title: Ulinastatin attenuates LPS-induced inflammation in mouse macrophage RAW264.7 cells by inhibiting the JNK/NF-κB signaling pathway and activating the PI3K/Akt/Nrf2 pathway
    Article Snippet: Paragraph title: Total RNA extraction and qRT-PCR analysis ... Total RNA was extracted with TRIzol Reagent and 4 μg total RNA was reverse-transcribed using random hexamers at 37°C with the M-MLV reverse transcriptase (Promega, Madison, USA). qRT-PCR was performed using SYBR Green (GeneCopoeia, USA) in a RT-PCR machine (QuantStudio 6 Flex System, Applied Biosystems, Foster City, CA, USA).

    Article Title: Anti-inflammatory effects of Viola yedoensis and the application of cell extraction methods for investigating bioactive constituents in macrophages
    Article Snippet: Paragraph title: Total RNA extraction and real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) ... The amplification and analysis were performed by a QuantStudio 6 Flex Real-time PCR System (Thermo Scientific, Rockford, IL, USA).

    Selection:

    Article Title: Bread Wheat With High Salinity and Sodicity Tolerance
    Article Snippet: Paragraph title: Candidate Gene Selection, Primer Design, and Gene Expression in Penultimate Leaves Under Control and Salinity ... Amplification were performed in a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher) with 3 min of 95°C followed by 40 cycles of 3s at 95°C, 20s at 60°C, and fluorescent acquisition at 60°C, followed by melt curve analysis.

    Agarose Gel Electrophoresis:

    Article Title: TRF2 positively regulates SULF2 expression increasing VEGF-A release and activity in tumor microenvironment
    Article Snippet: Quality of the extracted RNA was assessed by 1% agarose gel electrophoresis and from the A 260 nm /A 280 nm absorbance ratio (Nanodrop 1000, ThermoFisher Scientific). .. Real-time rt -PCRs were performed on the obtained cDNAs by using Fast power Syber green master mix (Apply Biosystem) on either QuantStudio 6-Flex (Thermo Fisher Scientific) or 7900HT Fast Real-Time PCR System (Applied Biosystem) thermocycler.

    Fluorescence:

    Article Title: Constriction of Retinal Venules to Endothelin-1: Obligatory Roles of ETA Receptors, Extracellular Calcium Entry, and Rho Kinase
    Article Snippet: .. Real-time PCR was performed by monitoring the increase in SYBR Green fluorescence using the Applied Biosystems QuantStudio 6 Flex Real-Time PCR System (Life Technologies Corporation, Carlsbad, CA, USA). ..

    Synthesized:

    Article Title: Antidepressant Effect of Fraxinus rhynchophylla Hance Extract in a Mouse Model of Chronic Stress-Induced Depression
    Article Snippet: The complementary deoxyribonucleic acid (cDNA) was synthesized by equalizing all samples to the same total amount of RNA and adding a synthetic reagent. .. Real-time PCR primers were loaded onto a MicroAmp Fast 96-well reaction plate (Applied Biosystems) with Power SYBR Green PCR Master Mix (Applied Biosystems) and mRNA was quantified using Quantstudio 6 Flex (Applied Biosystems by Life Technologies).

    Article Title: Anti-inflammatory effects of Viola yedoensis and the application of cell extraction methods for investigating bioactive constituents in macrophages
    Article Snippet: Total RNA extraction and real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) Total cellular RNA was extracted by easy-BLUE™ RNA extraction kit (iNtRON Biotech, Daejeon, Korea) in accordance with the protocol of manufacturer. cDNA was synthesized from 1 μg of total RNA using AccuPower®CycleScript RT PreMix (Bioneer, Daejeon, Korea). .. The amplification and analysis were performed by a QuantStudio 6 Flex Real-time PCR System (Thermo Scientific, Rockford, IL, USA).

    Isolation:

    Article Title: RNA–DNA hybrids promote the expansion of Friedreich's ataxia (GAA)n repeats via break-induced replication
    Article Snippet: RNA was isolated using a hot phenol–chloroform extraction ( ). .. Following the RT reaction, samples were treated with 0.5U RNase H at 37°C for 20 min. Quantitative PCR (qPCR) was performed in 10 μl volumes using SYBR Select Master Mix (ThermoFisher) on a QuantStudio 6 Flex RTPCR system (Applied Biosystems) in 384-well plates with four technical replicates per sample.

    Article Title: Bread Wheat With High Salinity and Sodicity Tolerance
    Article Snippet: Total RNA was isolated from penultimate leaves using Spectrum Plant Total RNA kit (Sigma) with an on-column DNase treatment. .. Amplification were performed in a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher) with 3 min of 95°C followed by 40 cycles of 3s at 95°C, 20s at 60°C, and fluorescent acquisition at 60°C, followed by melt curve analysis.

    Article Title: Exon Skipping in the RET Gene Encodes Novel Isoforms That Differentially Regulate RET Protein Signal Transduction *
    Article Snippet: Paragraph title: RNA Isolation, RT-PCR, and qPCR ... RT-PCR reactions with amplicons greater than 200 bp were performed using the Phusion high-fidelity DNA polymerase kit (New England Biolabs) following the instructions of the manufacturer. qPCR was performed using FastStart Universal SYBR Green Master Mix (Roche) and run on a QuantStudio 6 Flex real-time PCR system (Thermo Fisher Scientific).

    Article Title: TRF2 positively regulates SULF2 expression increasing VEGF-A release and activity in tumor microenvironment
    Article Snippet: Real-time PCR To quantify gene expression by real-time quantitative polymerase chain reaction (qPCR), total RNA was isolated from cell pellets by using TRIzol reagent (Ambion). .. Real-time rt -PCRs were performed on the obtained cDNAs by using Fast power Syber green master mix (Apply Biosystem) on either QuantStudio 6-Flex (Thermo Fisher Scientific) or 7900HT Fast Real-Time PCR System (Applied Biosystem) thermocycler.

    Article Title: Constriction of Retinal Venules to Endothelin-1: Obligatory Roles of ETA Receptors, Extracellular Calcium Entry, and Rho Kinase
    Article Snippet: Paragraph title: mRNA Isolation and Real-Time PCR Analysis ... Real-time PCR was performed by monitoring the increase in SYBR Green fluorescence using the Applied Biosystems QuantStudio 6 Flex Real-Time PCR System (Life Technologies Corporation, Carlsbad, CA, USA).

    Article Title: The targetable kinase PIM1 drives ALK inhibitor resistance in high-risk neuroblastoma independent of MYCN status
    Article Snippet: RT-qPCR analysis Total RNA was isolated from cell lines using the RNeasy Plus Mini Kit (Qiagen, Cat#74134). .. In total, 1 µg RNA was reverse transcribed using iScript Reverse Transcription Supermix (Bio-Rad, Cat#1708840) and 20 ng cDNA template was used for qPCR. cDNA was amplified with PowerUp SYBR Green Master Mix (Thermo Fisher Scientific, Cat#A25918) and 600 nM primers on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) under standard cycling conditions.

    Size-exclusion Chromatography:

    Article Title: TRF2 positively regulates SULF2 expression increasing VEGF-A release and activity in tumor microenvironment
    Article Snippet: Real-time rt -PCRs were performed on the obtained cDNAs by using Fast power Syber green master mix (Apply Biosystem) on either QuantStudio 6-Flex (Thermo Fisher Scientific) or 7900HT Fast Real-Time PCR System (Applied Biosystem) thermocycler. .. Standard qPCR thermal parameters were used: one cycle of 95°C for 10 min then 40 cycles of 95°C for 15 sec and 60°C for 1 min followed by dissociation curve (95°C for 15 s, 60°C for 1 min, 95°C for 15 s).

    Quantitative RT-PCR:

    Article Title: RNA–DNA hybrids promote the expansion of Friedreich's ataxia (GAA)n repeats via break-induced replication
    Article Snippet: Paragraph title: Strand-specific RT-qPCR ... Following the RT reaction, samples were treated with 0.5U RNase H at 37°C for 20 min. Quantitative PCR (qPCR) was performed in 10 μl volumes using SYBR Select Master Mix (ThermoFisher) on a QuantStudio 6 Flex RTPCR system (Applied Biosystems) in 384-well plates with four technical replicates per sample.

    Article Title: Ulinastatin attenuates LPS-induced inflammation in mouse macrophage RAW264.7 cells by inhibiting the JNK/NF-κB signaling pathway and activating the PI3K/Akt/Nrf2 pathway
    Article Snippet: .. Total RNA was extracted with TRIzol Reagent and 4 μg total RNA was reverse-transcribed using random hexamers at 37°C with the M-MLV reverse transcriptase (Promega, Madison, USA). qRT-PCR was performed using SYBR Green (GeneCopoeia, USA) in a RT-PCR machine (QuantStudio 6 Flex System, Applied Biosystems, Foster City, CA, USA). .. The relative levels of gene expression were calculated as ΔCt=Ct (gene)-Ct (reference).

    Article Title: Integrated microRNA and mRNA analysis in the pathogenic filamentous fungus Trichophyton rubrum
    Article Snippet: .. Validation of the expression of milRNAs and mRNA by qRT-PCR In order to validate the expression changes of miRNAs and their target genes between conidia and mycelia in T. rubrum , the relative expression levels of 9 randomly selected milRNAs and 20 target genes were analyzed by qRT-PCR on a QuantStudio™ 6 Flex qRT-PCR system (Applied Biosystems, Foster City, CA, USA). .. The milRNAs specific, stem-loop RT primers and 5.8S rRNA were supplied by Applied Biosystems (Applied Biosystems, Foster City, CA, USA) [ ].

    Article Title: The targetable kinase PIM1 drives ALK inhibitor resistance in high-risk neuroblastoma independent of MYCN status
    Article Snippet: Paragraph title: RT-qPCR analysis ... In total, 1 µg RNA was reverse transcribed using iScript Reverse Transcription Supermix (Bio-Rad, Cat#1708840) and 20 ng cDNA template was used for qPCR. cDNA was amplified with PowerUp SYBR Green Master Mix (Thermo Fisher Scientific, Cat#A25918) and 600 nM primers on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) under standard cycling conditions.

    Article Title: Integrated microRNA and mRNA analysis in the pathogenic filamentous fungus Trichophyton rubrum
    Article Snippet: .. In order to validate the expression changes of miRNAs and their target genes between conidia and mycelia in T. rubrum , the relative expression levels of 9 randomly selected milRNAs and 20 target genes were analyzed by qRT-PCR on a QuantStudio™ 6 Flex qRT-PCR system (Applied Biosystems, Foster City, CA, USA). .. The milRNAs specific, stem-loop RT primers and 5.8S rRNA were supplied by Applied Biosystems (Applied Biosystems, Foster City, CA, USA) [ ].

    Article Title: Anti-inflammatory effects of Viola yedoensis and the application of cell extraction methods for investigating bioactive constituents in macrophages
    Article Snippet: Paragraph title: Total RNA extraction and real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) ... The amplification and analysis were performed by a QuantStudio 6 Flex Real-time PCR System (Thermo Scientific, Rockford, IL, USA).

    Purification:

    Article Title: Bread Wheat With High Salinity and Sodicity Tolerance
    Article Snippet: The reaction contained 500 ng purified RNA from each sample in a final reaction volume of 20 µl, performed according to manufacturer’s instructions. .. Amplification were performed in a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher) with 3 min of 95°C followed by 40 cycles of 3s at 95°C, 20s at 60°C, and fluorescent acquisition at 60°C, followed by melt curve analysis.

    SYBR Green Assay:

    Article Title: Ulinastatin attenuates LPS-induced inflammation in mouse macrophage RAW264.7 cells by inhibiting the JNK/NF-κB signaling pathway and activating the PI3K/Akt/Nrf2 pathway
    Article Snippet: .. Total RNA was extracted with TRIzol Reagent and 4 μg total RNA was reverse-transcribed using random hexamers at 37°C with the M-MLV reverse transcriptase (Promega, Madison, USA). qRT-PCR was performed using SYBR Green (GeneCopoeia, USA) in a RT-PCR machine (QuantStudio 6 Flex System, Applied Biosystems, Foster City, CA, USA). .. The relative levels of gene expression were calculated as ΔCt=Ct (gene)-Ct (reference).

    Article Title: Antidepressant Effect of Fraxinus rhynchophylla Hance Extract in a Mouse Model of Chronic Stress-Induced Depression
    Article Snippet: .. Real-time PCR primers were loaded onto a MicroAmp Fast 96-well reaction plate (Applied Biosystems) with Power SYBR Green PCR Master Mix (Applied Biosystems) and mRNA was quantified using Quantstudio 6 Flex (Applied Biosystems by Life Technologies). .. The sequences of the real-time PCR primers were as follows: 5′-CTTGGCAACAGCACAGACC-3′ (forward), and 5′-GAGAAGTCCATGTCCGCAAT-3′ (reverse) for NF-κB p65 ; 5′- AGACCCTCACACTCAGAT CATCTTC-3′ (forward) and 5′-CCACTTGGTGGTTTGCTACGA-3′ (reverse) for TNFα ; 5′-GAGGATACCACTCCCAACAGACC-3′ (forward) and 5′-AAGTGCATCATCGTTGTTCATACA-3′ (reverse) for IL-6 ; 5′-AGACATGGAGTCATAGGCTCTG-3′ (forward) and 5′-CCATTTTCCTTCTT GTG GAGCA-3′ (reverse) for IL-12 ; and 5′-AAGGTGGTGAAGCAGGCA T-3′ (forward) and 5′-GGTCCAGGGTTTCTTACTCCT-3′ (reverse) for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) .

    Article Title: MicroRNA Expression Profiling in Psoriatic Arthritis
    Article Snippet: Real-time PCR was carried out in triplicate on a QuantStudio 6 Flex instrument (Applied Biosystems, Foster City, CA, USA). .. Real-time PCR were carried out in triplicate with PowerUp™ Sybr® Green reagent (Applied Biosystems, Foster City, CA, U.S.A).

    Article Title: Exposure–Response Associations of Household Air Pollution and Buccal Cell Telomere Length in Women Using Biomass Stoves
    Article Snippet: Each PCR reaction mixture contained a 5 -ng / μ L concentration of DNA and 1 × SYBR Green Master Mix (TaKaRa). .. The thermal cycling conditions were 95°C for 30 s followed by 40 cycles of 95°C for 5 s, 54°C for 30 s, and 72°C for 31 s. PCR reactions were conducted on a QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems™).

    Article Title: The Rosa genome provides new insights into the domestication of modern roses
    Article Snippet: .. 5.8S rRNA and miR156 expression were quantified on QuantStudio™ 6 Flex Real-Time PCR 384 (Applied Biosystems) using Fast SYBR® Green Master Mix kit (Roche Diagnostic) and specific primers ( ). ..

    Article Title: Exon Skipping in the RET Gene Encodes Novel Isoforms That Differentially Regulate RET Protein Signal Transduction *
    Article Snippet: .. RT-PCR reactions with amplicons greater than 200 bp were performed using the Phusion high-fidelity DNA polymerase kit (New England Biolabs) following the instructions of the manufacturer. qPCR was performed using FastStart Universal SYBR Green Master Mix (Roche) and run on a QuantStudio 6 Flex real-time PCR system (Thermo Fisher Scientific). .. Quantitative analyses were performed by calculating the ΔCt (Ct of transcript divided by Ct of the housekeeping gene actin ) for each gene analyzed and transforming that value to log2 .

    Article Title: TRF2 positively regulates SULF2 expression increasing VEGF-A release and activity in tumor microenvironment
    Article Snippet: Real-time rt -PCRs were performed on the obtained cDNAs by using Fast power Syber green master mix (Apply Biosystem) on either QuantStudio 6-Flex (Thermo Fisher Scientific) or 7900HT Fast Real-Time PCR System (Applied Biosystem) thermocycler. .. For each sample 5 μl of the 1:10 diluted cDNA was mixed with 0.5 μl of each primer (10 μM), 10 μl of the sybr green master mix and water at final volume of 20 μl.

    Article Title: Constriction of Retinal Venules to Endothelin-1: Obligatory Roles of ETA Receptors, Extracellular Calcium Entry, and Rho Kinase
    Article Snippet: .. Real-time PCR was performed by monitoring the increase in SYBR Green fluorescence using the Applied Biosystems QuantStudio 6 Flex Real-Time PCR System (Life Technologies Corporation, Carlsbad, CA, USA). ..

    Article Title: The targetable kinase PIM1 drives ALK inhibitor resistance in high-risk neuroblastoma independent of MYCN status
    Article Snippet: .. In total, 1 µg RNA was reverse transcribed using iScript Reverse Transcription Supermix (Bio-Rad, Cat#1708840) and 20 ng cDNA template was used for qPCR. cDNA was amplified with PowerUp SYBR Green Master Mix (Thermo Fisher Scientific, Cat#A25918) and 600 nM primers on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) under standard cycling conditions. ..

    Sequencing:

    Article Title: Bread Wheat With High Salinity and Sodicity Tolerance
    Article Snippet: Specific qPCR amplification was confirmed by obtaining a single distinct peak in melt curve analysis, and qPCR product sequencing. .. Amplification were performed in a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher) with 3 min of 95°C followed by 40 cycles of 3s at 95°C, 20s at 60°C, and fluorescent acquisition at 60°C, followed by melt curve analysis.

    Software:

    Article Title: MicroRNA Expression Profiling in Psoriatic Arthritis
    Article Snippet: Real-time PCR was carried out in triplicate on a QuantStudio 6 Flex instrument (Applied Biosystems, Foster City, CA, USA). .. Expression values were obtained by ΔCt method using QuantStudio Real-Time PCR system software v. 1.3.

    Article Title: Integrated microRNA and mRNA analysis in the pathogenic filamentous fungus Trichophyton rubrum
    Article Snippet: Validation of the expression of milRNAs and mRNA by qRT-PCR In order to validate the expression changes of miRNAs and their target genes between conidia and mycelia in T. rubrum , the relative expression levels of 9 randomly selected milRNAs and 20 target genes were analyzed by qRT-PCR on a QuantStudio™ 6 Flex qRT-PCR system (Applied Biosystems, Foster City, CA, USA). .. Gene specific primers for targets genes and 18S rRNA were designed by Primer Premier 5.0 software, with estimated melt temperature of 55–60 °C and amplification length of 90–150 bp.

    Article Title: Integrated microRNA and mRNA analysis in the pathogenic filamentous fungus Trichophyton rubrum
    Article Snippet: In order to validate the expression changes of miRNAs and their target genes between conidia and mycelia in T. rubrum , the relative expression levels of 9 randomly selected milRNAs and 20 target genes were analyzed by qRT-PCR on a QuantStudio™ 6 Flex qRT-PCR system (Applied Biosystems, Foster City, CA, USA). .. Gene specific primers for targets genes and 18S rRNA were designed by Primer Premier 5.0 software, with estimated melt temperature of 55–60 °C and amplification length of 90–150 bp.

    Amplification:

    Article Title: Bread Wheat With High Salinity and Sodicity Tolerance
    Article Snippet: .. Amplification were performed in a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher) with 3 min of 95°C followed by 40 cycles of 3s at 95°C, 20s at 60°C, and fluorescent acquisition at 60°C, followed by melt curve analysis. .. Three wheat genes, encoding actin, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and elongation factor 1-alpha (EF1a) were used together for normalization of target gene expression.

    Article Title: Antidepressant Effect of Fraxinus rhynchophylla Hance Extract in a Mouse Model of Chronic Stress-Induced Depression
    Article Snippet: Real-time PCR primers were loaded onto a MicroAmp Fast 96-well reaction plate (Applied Biosystems) with Power SYBR Green PCR Master Mix (Applied Biosystems) and mRNA was quantified using Quantstudio 6 Flex (Applied Biosystems by Life Technologies). .. Real-time PCR was performed by incubating the mixture and PCR thermal cycle parameters were as follows: 95°C for 10 min, 40 cycles of 60°C for 60 s, and 95°C for 15 s, and a melting curve from 60 to 95°C to ensure amplification of a single product.

    Article Title: MicroRNA Expression Profiling in Psoriatic Arthritis
    Article Snippet: 5 μ L of 1 : 10 diluted cDNAs was amplified in 20 μ L PCR reactions with 2x Fast Advanced Master Mix and TaqMan Advanced miRNA assays for hsa-miR-126-3p (477887_mir), hsa-miR-130a-3p (477851_mir), hsa-miR-151a-5p (478505_mir), or hsa-miR-148a-3p (477814_mir). .. Real-time PCR was carried out in triplicate on a QuantStudio 6 Flex instrument (Applied Biosystems, Foster City, CA, USA).

    Article Title: Exposure–Response Associations of Household Air Pollution and Buccal Cell Telomere Length in Women Using Biomass Stoves
    Article Snippet: Each sample was amplified for telomeric DNA and for beta‐globin using the SYBR® Green method. .. The thermal cycling conditions were 95°C for 30 s followed by 40 cycles of 95°C for 5 s, 54°C for 30 s, and 72°C for 31 s. PCR reactions were conducted on a QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems™).

    Article Title: Integrated microRNA and mRNA analysis in the pathogenic filamentous fungus Trichophyton rubrum
    Article Snippet: Validation of the expression of milRNAs and mRNA by qRT-PCR In order to validate the expression changes of miRNAs and their target genes between conidia and mycelia in T. rubrum , the relative expression levels of 9 randomly selected milRNAs and 20 target genes were analyzed by qRT-PCR on a QuantStudio™ 6 Flex qRT-PCR system (Applied Biosystems, Foster City, CA, USA). .. Gene specific primers for targets genes and 18S rRNA were designed by Primer Premier 5.0 software, with estimated melt temperature of 55–60 °C and amplification length of 90–150 bp.

    Article Title: The targetable kinase PIM1 drives ALK inhibitor resistance in high-risk neuroblastoma independent of MYCN status
    Article Snippet: .. In total, 1 µg RNA was reverse transcribed using iScript Reverse Transcription Supermix (Bio-Rad, Cat#1708840) and 20 ng cDNA template was used for qPCR. cDNA was amplified with PowerUp SYBR Green Master Mix (Thermo Fisher Scientific, Cat#A25918) and 600 nM primers on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) under standard cycling conditions. ..

    Article Title: Integrated microRNA and mRNA analysis in the pathogenic filamentous fungus Trichophyton rubrum
    Article Snippet: In order to validate the expression changes of miRNAs and their target genes between conidia and mycelia in T. rubrum , the relative expression levels of 9 randomly selected milRNAs and 20 target genes were analyzed by qRT-PCR on a QuantStudio™ 6 Flex qRT-PCR system (Applied Biosystems, Foster City, CA, USA). .. Gene specific primers for targets genes and 18S rRNA were designed by Primer Premier 5.0 software, with estimated melt temperature of 55–60 °C and amplification length of 90–150 bp.

    Article Title: Anti-inflammatory effects of Viola yedoensis and the application of cell extraction methods for investigating bioactive constituents in macrophages
    Article Snippet: .. The amplification and analysis were performed by a QuantStudio 6 Flex Real-time PCR System (Thermo Scientific, Rockford, IL, USA). .. The results of real-time RT-PCR were presented as inflammatory mediator gene induction fold, which were calculated using β-actin as an internal control.

    Construct:

    Article Title: Bread Wheat With High Salinity and Sodicity Tolerance
    Article Snippet: Amplification were performed in a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher) with 3 min of 95°C followed by 40 cycles of 3s at 95°C, 20s at 60°C, and fluorescent acquisition at 60°C, followed by melt curve analysis. .. Purified PCR products of target genes, covering six orders of magnitude, were used to construct a standard curve in relation to the cycle threshold (Ct) value from which the actual copy number per microgram of RNA was obtained.

    Expressing:

    Article Title: Bread Wheat With High Salinity and Sodicity Tolerance
    Article Snippet: Paragraph title: Candidate Gene Selection, Primer Design, and Gene Expression in Penultimate Leaves Under Control and Salinity ... Amplification were performed in a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher) with 3 min of 95°C followed by 40 cycles of 3s at 95°C, 20s at 60°C, and fluorescent acquisition at 60°C, followed by melt curve analysis.

    Article Title: Ulinastatin attenuates LPS-induced inflammation in mouse macrophage RAW264.7 cells by inhibiting the JNK/NF-κB signaling pathway and activating the PI3K/Akt/Nrf2 pathway
    Article Snippet: Total RNA was extracted with TRIzol Reagent and 4 μg total RNA was reverse-transcribed using random hexamers at 37°C with the M-MLV reverse transcriptase (Promega, Madison, USA). qRT-PCR was performed using SYBR Green (GeneCopoeia, USA) in a RT-PCR machine (QuantStudio 6 Flex System, Applied Biosystems, Foster City, CA, USA). .. The relative levels of gene expression were calculated as ΔCt=Ct (gene)-Ct (reference).

    Article Title: MicroRNA Expression Profiling in Psoriatic Arthritis
    Article Snippet: The mean of Ct for miR-16-5p and miR-26a-5p expression was used to normalize miRNA expression. .. Real-time PCR was carried out in triplicate on a QuantStudio 6 Flex instrument (Applied Biosystems, Foster City, CA, USA).

    Article Title: The Rosa genome provides new insights into the domestication of modern roses
    Article Snippet: .. 5.8S rRNA and miR156 expression were quantified on QuantStudio™ 6 Flex Real-Time PCR 384 (Applied Biosystems) using Fast SYBR® Green Master Mix kit (Roche Diagnostic) and specific primers ( ). ..

    Article Title: TRF2 positively regulates SULF2 expression increasing VEGF-A release and activity in tumor microenvironment
    Article Snippet: Real-time PCR To quantify gene expression by real-time quantitative polymerase chain reaction (qPCR), total RNA was isolated from cell pellets by using TRIzol reagent (Ambion). .. Real-time rt -PCRs were performed on the obtained cDNAs by using Fast power Syber green master mix (Apply Biosystem) on either QuantStudio 6-Flex (Thermo Fisher Scientific) or 7900HT Fast Real-Time PCR System (Applied Biosystem) thermocycler.

    Article Title: Integrated microRNA and mRNA analysis in the pathogenic filamentous fungus Trichophyton rubrum
    Article Snippet: .. Validation of the expression of milRNAs and mRNA by qRT-PCR In order to validate the expression changes of miRNAs and their target genes between conidia and mycelia in T. rubrum , the relative expression levels of 9 randomly selected milRNAs and 20 target genes were analyzed by qRT-PCR on a QuantStudio™ 6 Flex qRT-PCR system (Applied Biosystems, Foster City, CA, USA). .. The milRNAs specific, stem-loop RT primers and 5.8S rRNA were supplied by Applied Biosystems (Applied Biosystems, Foster City, CA, USA) [ ].

    Article Title: Integrated microRNA and mRNA analysis in the pathogenic filamentous fungus Trichophyton rubrum
    Article Snippet: .. In order to validate the expression changes of miRNAs and their target genes between conidia and mycelia in T. rubrum , the relative expression levels of 9 randomly selected milRNAs and 20 target genes were analyzed by qRT-PCR on a QuantStudio™ 6 Flex qRT-PCR system (Applied Biosystems, Foster City, CA, USA). .. The milRNAs specific, stem-loop RT primers and 5.8S rRNA were supplied by Applied Biosystems (Applied Biosystems, Foster City, CA, USA) [ ].

    Polymerase Chain Reaction:

    Article Title: Bread Wheat With High Salinity and Sodicity Tolerance
    Article Snippet: Amplification were performed in a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher) with 3 min of 95°C followed by 40 cycles of 3s at 95°C, 20s at 60°C, and fluorescent acquisition at 60°C, followed by melt curve analysis. .. Purified PCR products of target genes, covering six orders of magnitude, were used to construct a standard curve in relation to the cycle threshold (Ct) value from which the actual copy number per microgram of RNA was obtained.

    Article Title: Antidepressant Effect of Fraxinus rhynchophylla Hance Extract in a Mouse Model of Chronic Stress-Induced Depression
    Article Snippet: .. Real-time PCR primers were loaded onto a MicroAmp Fast 96-well reaction plate (Applied Biosystems) with Power SYBR Green PCR Master Mix (Applied Biosystems) and mRNA was quantified using Quantstudio 6 Flex (Applied Biosystems by Life Technologies). .. The sequences of the real-time PCR primers were as follows: 5′-CTTGGCAACAGCACAGACC-3′ (forward), and 5′-GAGAAGTCCATGTCCGCAAT-3′ (reverse) for NF-κB p65 ; 5′- AGACCCTCACACTCAGAT CATCTTC-3′ (forward) and 5′-CCACTTGGTGGTTTGCTACGA-3′ (reverse) for TNFα ; 5′-GAGGATACCACTCCCAACAGACC-3′ (forward) and 5′-AAGTGCATCATCGTTGTTCATACA-3′ (reverse) for IL-6 ; 5′-AGACATGGAGTCATAGGCTCTG-3′ (forward) and 5′-CCATTTTCCTTCTT GTG GAGCA-3′ (reverse) for IL-12 ; and 5′-AAGGTGGTGAAGCAGGCA T-3′ (forward) and 5′-GGTCCAGGGTTTCTTACTCCT-3′ (reverse) for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) .

    Article Title: MicroRNA Expression Profiling in Psoriatic Arthritis
    Article Snippet: 5 μ L of 1 : 10 diluted cDNAs was amplified in 20 μ L PCR reactions with 2x Fast Advanced Master Mix and TaqMan Advanced miRNA assays for hsa-miR-126-3p (477887_mir), hsa-miR-130a-3p (477851_mir), hsa-miR-151a-5p (478505_mir), or hsa-miR-148a-3p (477814_mir). .. Real-time PCR was carried out in triplicate on a QuantStudio 6 Flex instrument (Applied Biosystems, Foster City, CA, USA).

    Article Title: A novel HRM assay for the simultaneous detection and differentiation of eight poxviruses of medical and veterinary importance
    Article Snippet: .. Thus, using the same PCR mix and protocol as in the BioRad CFX96 instrument, PCR and melting curve acquisition analysis was also conducted on the LightCycler® 480 Real-Time PCR Systems (Roche), QuantStudio™ 6 Flex Real-Time PCR System (Life Technologies), and Rotor-Gene Q Real-Time PCR Cycler (Qiagen). ..

    Article Title: Exposure–Response Associations of Household Air Pollution and Buccal Cell Telomere Length in Women Using Biomass Stoves
    Article Snippet: .. The thermal cycling conditions were 95°C for 30 s followed by 40 cycles of 95°C for 5 s, 54°C for 30 s, and 72°C for 31 s. PCR reactions were conducted on a QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems™). ..

    Article Title: Anti-inflammatory effects of Viola yedoensis and the application of cell extraction methods for investigating bioactive constituents in macrophages
    Article Snippet: The following PCR conditions were applied: TNF-α, IL-6, IL-1β, iNOS, COX-2, HO-1, and β-actin, with 40 cycles of 94 °C for 15 s and 60 °C for 1 min [ ]. .. The amplification and analysis were performed by a QuantStudio 6 Flex Real-time PCR System (Thermo Scientific, Rockford, IL, USA).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: The targetable kinase PIM1 drives ALK inhibitor resistance in high-risk neuroblastoma independent of MYCN status
    Article Snippet: .. In total, 1 µg RNA was reverse transcribed using iScript Reverse Transcription Supermix (Bio-Rad, Cat1708840) and 20 ng cDNA template was used for qPCR. cDNA was amplified with PowerUp SYBR Green Master Mix (Thermo Fisher Scientific, Cat#A25918) and 600 nM primers on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) under standard cycling conditions. ..

    Spectrophotometry:

    Article Title: Constriction of Retinal Venules to Endothelin-1: Obligatory Roles of ETA Receptors, Extracellular Calcium Entry, and Rho Kinase
    Article Snippet: RNA and cDNA were quantified using a Nanodrop spectrophotometer (BioTek, Winooski, VT, USA), and sequentially processed using real-time PCR reagents (PowerUp SYBR Green Master Mix, Thermo Fisher Scientific). .. Real-time PCR was performed by monitoring the increase in SYBR Green fluorescence using the Applied Biosystems QuantStudio 6 Flex Real-Time PCR System (Life Technologies Corporation, Carlsbad, CA, USA).

    Concentration Assay:

    Article Title: Exposure–Response Associations of Household Air Pollution and Buccal Cell Telomere Length in Women Using Biomass Stoves
    Article Snippet: Each PCR reaction mixture contained a 5 -ng / μ L concentration of DNA and 1 × SYBR Green Master Mix (TaKaRa). .. The thermal cycling conditions were 95°C for 30 s followed by 40 cycles of 95°C for 5 s, 54°C for 30 s, and 72°C for 31 s. PCR reactions were conducted on a QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems™).

    Article Title: The Rosa genome provides new insights into the domestication of modern roses
    Article Snippet: RNA concentration was measured on a NanoDrop ND-1000 Micro-Volume (NanoDrop Technologies) before and after DNase treatment. .. 5.8S rRNA and miR156 expression were quantified on QuantStudio™ 6 Flex Real-Time PCR 384 (Applied Biosystems) using Fast SYBR® Green Master Mix kit (Roche Diagnostic) and specific primers ( ).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: RNA–DNA hybrids promote the expansion of Friedreich's ataxia (GAA)n repeats via break-induced replication
    Article Snippet: .. Following the RT reaction, samples were treated with 0.5U RNase H at 37°C for 20 min. Quantitative PCR (qPCR) was performed in 10 μl volumes using SYBR Select Master Mix (ThermoFisher) on a QuantStudio 6 Flex RTPCR system (Applied Biosystems) in 384-well plates with four technical replicates per sample. ..

    Article Title: Ulinastatin attenuates LPS-induced inflammation in mouse macrophage RAW264.7 cells by inhibiting the JNK/NF-κB signaling pathway and activating the PI3K/Akt/Nrf2 pathway
    Article Snippet: .. Total RNA was extracted with TRIzol Reagent and 4 μg total RNA was reverse-transcribed using random hexamers at 37°C with the M-MLV reverse transcriptase (Promega, Madison, USA). qRT-PCR was performed using SYBR Green (GeneCopoeia, USA) in a RT-PCR machine (QuantStudio 6 Flex System, Applied Biosystems, Foster City, CA, USA). .. The relative levels of gene expression were calculated as ΔCt=Ct (gene)-Ct (reference).

    Article Title: The Rosa genome provides new insights into the domestication of modern roses
    Article Snippet: Small RNA quantification was performed using stem-loop RT-PCR as previously described . .. 5.8S rRNA and miR156 expression were quantified on QuantStudio™ 6 Flex Real-Time PCR 384 (Applied Biosystems) using Fast SYBR® Green Master Mix kit (Roche Diagnostic) and specific primers ( ).

    Article Title: Exon Skipping in the RET Gene Encodes Novel Isoforms That Differentially Regulate RET Protein Signal Transduction *
    Article Snippet: .. RT-PCR reactions with amplicons greater than 200 bp were performed using the Phusion high-fidelity DNA polymerase kit (New England Biolabs) following the instructions of the manufacturer. qPCR was performed using FastStart Universal SYBR Green Master Mix (Roche) and run on a QuantStudio 6 Flex real-time PCR system (Thermo Fisher Scientific). .. Quantitative analyses were performed by calculating the ΔCt (Ct of transcript divided by Ct of the housekeeping gene actin ) for each gene analyzed and transforming that value to log2 .

    Article Title: Anti-inflammatory effects of Viola yedoensis and the application of cell extraction methods for investigating bioactive constituents in macrophages
    Article Snippet: Paragraph title: Total RNA extraction and real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) ... The amplification and analysis were performed by a QuantStudio 6 Flex Real-time PCR System (Thermo Scientific, Rockford, IL, USA).

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