abc elite kit  (Vector Laboratories)


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    Name:
    VECTASTAIN Elite ABC HRP Kit Peroxidase Standard
    Description:
    VECTASTAIN Elite ABC Peroxidase Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes VECTASTAIN Elite ABC SystemAdvanced avidin biotin technology The Elite ABC complex is smaller very uniform and highly active allowing more accessibility for binding to a biotinylated targetHighest sensitivity low background The VECTASTAIN Elite ABC system is the most sensitive avidin biotin complex based peroxidase system The Elite ABC series is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background Cost effective Higher sensitivity leads to lower cost per slide Available without Standard kit or with biotinylated species specific or universal secondary antibodies Available in Ready To Use R T U formats Prediluted stabilized working solutions of Elite ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN Elite ABC Staining Kit reagents VECTASTAIN Elite ABC Kit Components 2 ml Reagent A2 ml Reagent BReagent A and B when mixed form the ABC peroxidase complex Kit contains sufficient reagents to stain 500 1000 sections The Avidin Biotin Complex MethodThe VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin Biotinylated enzyme Complex This ABC complex takes advantage of two important properties of avidin 1 an extraordinarily high affinity for biotin over one million times higher than antibody for most antigens and 2 four biotin binding sites These properties allow macromolecular complexes ABCs to be formed by mixing Avidin DH Reagent A with its paired biotinylated enzyme Reagent B prior to use The ABC reagent once formed remains stable for many hours after formation and can be used for several days after preparation The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated This property gives the avidin biotin complex ABC method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed Biotinylated primary antibodies secondaries lectins neuronal tracers nucleic acids and ligands can be effectively visualized in applications such as Tissue stainingMultiple labeling Multiplex IHC Western blottingSouthern and northern blottingIn situ hybridization detection ISH Enzyme immunoassays ELISA Neuronal tracingAll applications benefit from the high sensitivity low background reproducibility and economy of the VECTASTAIN ABC system
    Catalog Number:
    PK-6100
    Price:
    None
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Reactivity:
    No antibody included
    Size:
    1 Kit
    Buy from Supplier


    Structured Review

    Vector Laboratories abc elite kit
    VECTASTAIN Elite ABC HRP Kit Peroxidase Standard
    VECTASTAIN Elite ABC Peroxidase Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes VECTASTAIN Elite ABC SystemAdvanced avidin biotin technology The Elite ABC complex is smaller very uniform and highly active allowing more accessibility for binding to a biotinylated targetHighest sensitivity low background The VECTASTAIN Elite ABC system is the most sensitive avidin biotin complex based peroxidase system The Elite ABC series is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background Cost effective Higher sensitivity leads to lower cost per slide Available without Standard kit or with biotinylated species specific or universal secondary antibodies Available in Ready To Use R T U formats Prediluted stabilized working solutions of Elite ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN Elite ABC Staining Kit reagents VECTASTAIN Elite ABC Kit Components 2 ml Reagent A2 ml Reagent BReagent A and B when mixed form the ABC peroxidase complex Kit contains sufficient reagents to stain 500 1000 sections The Avidin Biotin Complex MethodThe VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin Biotinylated enzyme Complex This ABC complex takes advantage of two important properties of avidin 1 an extraordinarily high affinity for biotin over one million times higher than antibody for most antigens and 2 four biotin binding sites These properties allow macromolecular complexes ABCs to be formed by mixing Avidin DH Reagent A with its paired biotinylated enzyme Reagent B prior to use The ABC reagent once formed remains stable for many hours after formation and can be used for several days after preparation The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated This property gives the avidin biotin complex ABC method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed Biotinylated primary antibodies secondaries lectins neuronal tracers nucleic acids and ligands can be effectively visualized in applications such as Tissue stainingMultiple labeling Multiplex IHC Western blottingSouthern and northern blottingIn situ hybridization detection ISH Enzyme immunoassays ELISA Neuronal tracingAll applications benefit from the high sensitivity low background reproducibility and economy of the VECTASTAIN ABC system
    https://www.bioz.com/result/abc elite kit/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    abc elite kit - by Bioz Stars, 2021-04
    99/100 stars

    Images

    Related Articles

    Immunohistochemistry:

    Article Title: Natural Reassortants of Potentially Zoonotic Avian Influenza Viruses H5N1 and H9N2 from Egypt Display Distinct Pathogenic Phenotypes in Experimentally Infected Chickens and Ferrets
    Article Snippet: Tissues were fixed in 10% neutral buffered formalin for 21 days, processed, and embedded in paraffin wax using a Leica ASP 300S fully enclosed tissue processor (Leica Biosystems, Nussloch, Germany), sectioned to a thickness of 2 to 4 μm using a Hyrax M55 electronic rotary microtome (Carl Zeiss Microimaging GmbH, Jena, Germany), mounted on Superfrost Plus glass slides (Menzel Gläser, Braunschweig, Germany), stained with hematoxylin and eosin using a Medite TST 44.000C automatic tissue stainer (Medite, Burgdorf, Germany), and screened for histopathological changes using an Axio Imager M2 microscope equipped with 10×, 20×, and 40× Plan Neofluar objectives and an AxioCam ICc3 3.3-megapixel digital camera (Carl Zeiss Microscopy GmbH, Jena, Germany). .. Immunohistochemistry was performed on serial sections to detect influenza A virus antigen using the avidin-biotin-peroxidase complex method (Vectastain PK 6100; Vector Laboratories, Burlingame, CA, USA) with citric buffer (10 mM, pH 6.0) pretreatment, a polyclonal rabbit anti-influenza A virus FPV/Rostock/34 nucleoprotein antiserum (diluted 1:750) , 3-amino-9-ethylcarbazol as the chromogen (Agilent Technologies, Santa Clara, CA, USA), and hematoxylin counterstain. .. The specificity of the immunohistochemical reaction was confirmed by the use of negative tissues from noninfected chicken and ferret archival diagnostic cases, as well as validated positive avian tissues from the recent H5N8 outbreak ( ).

    Article Title: Modulation of lethal HPAIV H5N8 clade 2.3.4.4B infection in AIV pre-exposed mallards
    Article Snippet: Hematoxylin and eosin stained sections were evaluated for histopathological lesions using a light microscope, and the severity of parenchymal necrotizing inflammation, as well as lymphatic necrosis, apoptosis and/or depletion in the lymphatic organs was scored on an ordinal 4-step scale (0 = unchanged, 1 = mild, 2 = moderate, 3 = severe). .. Immunohistochemistry was employed to detect influenza A virus matrix protein using the avidin–biotin-peroxidase-complex method (Vectastain PK 6100; Vector Laboratories, Burlingame, CA, USA) with citric buffer (10 mM, pH 6.0) pretreatment, a monoclonal antibody (mAb) directed against an epitope of the influenza A virus matrix protein (ATCC clone HB-64), 3-amino-9-ethylcarbazol chromogen (Agilent Technologies, Santa Clara, CA, USA), and hematoxylin counterstain [ ]. ..

    Avidin-Biotin Assay:

    Article Title: Natural Reassortants of Potentially Zoonotic Avian Influenza Viruses H5N1 and H9N2 from Egypt Display Distinct Pathogenic Phenotypes in Experimentally Infected Chickens and Ferrets
    Article Snippet: Tissues were fixed in 10% neutral buffered formalin for 21 days, processed, and embedded in paraffin wax using a Leica ASP 300S fully enclosed tissue processor (Leica Biosystems, Nussloch, Germany), sectioned to a thickness of 2 to 4 μm using a Hyrax M55 electronic rotary microtome (Carl Zeiss Microimaging GmbH, Jena, Germany), mounted on Superfrost Plus glass slides (Menzel Gläser, Braunschweig, Germany), stained with hematoxylin and eosin using a Medite TST 44.000C automatic tissue stainer (Medite, Burgdorf, Germany), and screened for histopathological changes using an Axio Imager M2 microscope equipped with 10×, 20×, and 40× Plan Neofluar objectives and an AxioCam ICc3 3.3-megapixel digital camera (Carl Zeiss Microscopy GmbH, Jena, Germany). .. Immunohistochemistry was performed on serial sections to detect influenza A virus antigen using the avidin-biotin-peroxidase complex method (Vectastain PK 6100; Vector Laboratories, Burlingame, CA, USA) with citric buffer (10 mM, pH 6.0) pretreatment, a polyclonal rabbit anti-influenza A virus FPV/Rostock/34 nucleoprotein antiserum (diluted 1:750) , 3-amino-9-ethylcarbazol as the chromogen (Agilent Technologies, Santa Clara, CA, USA), and hematoxylin counterstain. .. The specificity of the immunohistochemical reaction was confirmed by the use of negative tissues from noninfected chicken and ferret archival diagnostic cases, as well as validated positive avian tissues from the recent H5N8 outbreak ( ).

    Article Title: Modulation of lethal HPAIV H5N8 clade 2.3.4.4B infection in AIV pre-exposed mallards
    Article Snippet: Hematoxylin and eosin stained sections were evaluated for histopathological lesions using a light microscope, and the severity of parenchymal necrotizing inflammation, as well as lymphatic necrosis, apoptosis and/or depletion in the lymphatic organs was scored on an ordinal 4-step scale (0 = unchanged, 1 = mild, 2 = moderate, 3 = severe). .. Immunohistochemistry was employed to detect influenza A virus matrix protein using the avidin–biotin-peroxidase-complex method (Vectastain PK 6100; Vector Laboratories, Burlingame, CA, USA) with citric buffer (10 mM, pH 6.0) pretreatment, a monoclonal antibody (mAb) directed against an epitope of the influenza A virus matrix protein (ATCC clone HB-64), 3-amino-9-ethylcarbazol chromogen (Agilent Technologies, Santa Clara, CA, USA), and hematoxylin counterstain [ ]. ..

    Article Title: Two monoclonal antibodies against glycoprotein Gn protect mice from Rift Valley Fever challenge by cooperative effects
    Article Snippet: .. Immunohistology was performed with the avidin–biotin–peroxidase complex method (ABC, Elite PK6100; Vector Laboratories, Burlingame, CA, USA) with 3-amino-9-ethylcarbazole (AEC, Dako, Glostrup, Denmark) as chromogen and hematoxylin counterstain. .. The primary antibody used for the detection of RVFV nucleoprotein was a heat-inactivated serum of a sheep immunized with recombinant RVFV MP12-strain nucleoprotein (internal code: S24NP) in a dilution of 1:4000.

    Article Title: Productive Propagation of Rift Valley Fever Phlebovirus Vaccine Strain MP-12 in Rousettus aegyptiacus Fruit Bats
    Article Snippet: Tissue specimens were fixed in 4% neutral buffered formaldehyde, processed, embedded in paraffin wax, sectioned at 2–4 μm thickness, and stained with hematoxylin and eosin. .. Immunohistology was performed using a mouse monoclonal antibody against the RVFV Gc-protein (clone: GC9A9) [ ], the avidin–biotin–peroxidase complex method (ABC, Elite PK6100; Vector Laboratories, Burlingame, CA, USA) with 3-amino-9-ethylcarbazole (AEC, Dako, Glostrup, Denmark) as chromogen and hematoxylin counterstain. ..

    Produced:

    Article Title: The Importance of Titrating Antibodies for Immunocytochemical Methods
    Article Snippet: Biotinylated tyramine was described for ICC by and later adapted for fluorescence by . .. Brain sections as obtained in Basic Protocol 1, steps 1 to 15 Primary antibody Biotinylated secondary antibody Vectastain Elite ABC Kit (Standard; Vector Laboratories, cat. no. PK-6100) including solution A and Solution B Biotinylated tyramine produced as outlined in . .. This reagent can be purchased from a number of suppliers (such as ThermoFisher Scientific) but concentrations must be determined empirically.

    Immunoperoxidase Staining:

    Article Title: Matrix Gla Protein Binds to Fibronectin and Enhances Cell Attachment and Spreading on Fibronectin
    Article Snippet: .. Immunoperoxidase staining followed the Vectastain ABC Elite kit instructions (Vector labs). .. Sections were blocked with buffer containing normal horse serum at 1.5% then incubated with rabbit anti-MGP at 20 μ g/mL or mouse antifibronectin monoclonal antibody (clone IST-3 Sigma) at 1 : 5000 dilution.

    Incubation:

    Article Title: Early life trauma increases threat response of peri-weaning rats, reduction of axo-somatic synapses formed by parvalbumin cells and perineuronal net in the basolateral nucleus of amygdala
    Article Snippet: After a ~48 hr incubation at room temperature with the mouse anti-PV antibody diluted in PBS-BSA-Azide (1:10,000), tissue was washed with PBS and incubated for 1 hr in biotinylated goat-anti-mouse IgG secondary antibody (Vector laboratories, Burlingame, CA, cat# BA-9200, dilution 1:200). .. Tissue was then washed in 0.01M PBS, incubated in a solution of the Vectastain Elite ABC HRP kit (Vector Laboratories, Burlingame, CA, cat# PK-6100), washed with 0.01M PBS, and incubated for 9 min in a filtered solution of 3’3-diaminobenzidine tetrahydrochloride (DAB; 10mg tablet Sigma Aldrich in 44ml of PBS buffer), catalyzed by 0.003% hydrogen peroxide. ..

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  • 99
    Vector Laboratories aec
    Human coronary lesions stained for CD 31, HAM 56, VCAM-1, 90.45, and HUTS-21. Human coronary lesions were stained with CD 31 ( a ), HAM 56 ( b ), 90.45 ( c ), or VCAM-1 ( d ). Antibodies in a–d were viewed with <t>ABC</t> and <t>AEC.</t> These four panels show that sections containing macrophages display endothelial CS-1 as detected by the 90.45 antibody but not VCAM-1 staining. In a separate study, the luminal endothelium of coronary vessels was stained for 90.45 ( e ) and HUTS-21 ( f ). Antibodies in e and f were viewed with DAB. Areas that stained most positively for 90.45 wer e mirrored by HUTS-21 ( arrow ), whereas areas of lesser staining were also parallel between the two antibodies ( double arrow ). ×1000. DAB, diaminobenzidine; ABC , avidin/biotinylated horseradish peroxide macromolecular complex; AEC , amino-9-ethyl carbazole.
    Aec, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aec/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    98
    Vector Laboratories vectastain elite abc
    Immunohistochemical and subcellular localization of transgenic survivin expression. ( a ) Five-micrometer tissue sections were cut from fresh-frozen skin of K14-survivin transgenic mice (K14-survivin) or control nontransgenic (non-TG) littermates, fixed in ice-cold acetone, and analyzed by immunohistochemistry with Ab against survivin or a control Ab to EGFP. Binding of the primary Ab’s was visualized with a goat anti-rabbit polyclonal Ab using <t>Vectastain</t> Elite <t>ABC</t> and AEC peroxidase substrate kits. ( b ) Subcellular localization of transgenic survivin. Keratinocytes isolated from K14-survivin transgenic mice (K14-survivin) or nontransgenic littermates (non-TG) were adhered to glass coverslips, fixed in methanol-acetone, and incubated with an Ab to survivin, followed by Texas red–conjugated goat anti-rabbit Ab. Nuclei were stained with Hoechst 33342. Image merging analysis is shown.
    Vectastain Elite Abc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectastain elite abc/product/Vector Laboratories
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    96
    Vector Laboratories biotinylated goat anti rabbit igg
    Detection of GNAQ in TG neurons during latency-reactivation cycle. TG were collected from 3 uninfected calves (U) (A), latently infected calves (L) (B), or latently infected calves treated with DEX for 6 h to initiate reactivation from latency (DEX) (C). Thin sections were cut from formalin-fixed, paraffin-embedded TG sections. The GNAQ antibody (ab75825; Abcam) was diluted 1:450. <t>Biotinylated</t> goat anti-rabbit <t>IgG</t> (Vector Laboratories) was used as a secondary antibody. Arrows denote GNAQ + neurons in the respective samples. (D) The percentage of GNAQ-positive neurons from 296 uninfected neurons, 241 latently infected neurons, and 209 TG neurons at 6 h after latently infected calves were treated with DEX. An asterisk denotes significant differences ( P
    Biotinylated Goat Anti Rabbit Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti rabbit igg/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Human coronary lesions stained for CD 31, HAM 56, VCAM-1, 90.45, and HUTS-21. Human coronary lesions were stained with CD 31 ( a ), HAM 56 ( b ), 90.45 ( c ), or VCAM-1 ( d ). Antibodies in a–d were viewed with ABC and AEC. These four panels show that sections containing macrophages display endothelial CS-1 as detected by the 90.45 antibody but not VCAM-1 staining. In a separate study, the luminal endothelium of coronary vessels was stained for 90.45 ( e ) and HUTS-21 ( f ). Antibodies in e and f were viewed with DAB. Areas that stained most positively for 90.45 wer e mirrored by HUTS-21 ( arrow ), whereas areas of lesser staining were also parallel between the two antibodies ( double arrow ). ×1000. DAB, diaminobenzidine; ABC , avidin/biotinylated horseradish peroxide macromolecular complex; AEC , amino-9-ethyl carbazole.

    Journal: Journal of Clinical Investigation

    Article Title: Minimally modified low-density lipoprotein induces monocyte adhesion to endothelial connecting segment-1 by activating ?1 integrin

    doi:

    Figure Lengend Snippet: Human coronary lesions stained for CD 31, HAM 56, VCAM-1, 90.45, and HUTS-21. Human coronary lesions were stained with CD 31 ( a ), HAM 56 ( b ), 90.45 ( c ), or VCAM-1 ( d ). Antibodies in a–d were viewed with ABC and AEC. These four panels show that sections containing macrophages display endothelial CS-1 as detected by the 90.45 antibody but not VCAM-1 staining. In a separate study, the luminal endothelium of coronary vessels was stained for 90.45 ( e ) and HUTS-21 ( f ). Antibodies in e and f were viewed with DAB. Areas that stained most positively for 90.45 wer e mirrored by HUTS-21 ( arrow ), whereas areas of lesser staining were also parallel between the two antibodies ( double arrow ). ×1000. DAB, diaminobenzidine; ABC , avidin/biotinylated horseradish peroxide macromolecular complex; AEC , amino-9-ethyl carbazole.

    Article Snippet: Antibodies were viewed using ABC (catalog no. PK6100; Vector Laboratories) and AEC (catalog no. SO1; BiomedaFoster City, California, USA) kits.

    Techniques: Staining, Avidin-Biotin Assay

    Histopathological findings in the liver of a Rousettus aegyptiacus fruit bat immunized with the MP-12 vaccine strain at day seven post immunization. ( A ) Histopathology shows few, randomly distributed foci of hepatocellular necrosis and loss with macrophage and lymphocyte infiltration (arrow). Furthermore, the hepatocytes display moderate, coalescing to diffuse, floccular cytoplasmic vacuolization, interpreted as a species-specific, relatively high level of glycogen storage. Hematoxylin-eosin. Bar = 100 μm; ( B ) Immunohistochemistry for Rift Valley fever phlebovirus (RVFV) Gc antigen reveals minor amounts of intra- and extracellular, strongly immunoreactive granula within the lesions (arrow), interpreted as debris remaining after virus-induced hepatocellular death. Immunohistochemistry, monoclonal mouse anti-RVFV Gc-protein antibody, avidin-biotin-peroxidase-complex method, 3-amino-9-ethyl-carbazol chromogen (red), hematoxylin counterstain (blue). Bar = 20 μm.

    Journal: Viruses

    Article Title: Productive Propagation of Rift Valley Fever Phlebovirus Vaccine Strain MP-12 in Rousettus aegyptiacus Fruit Bats

    doi: 10.3390/v10120681

    Figure Lengend Snippet: Histopathological findings in the liver of a Rousettus aegyptiacus fruit bat immunized with the MP-12 vaccine strain at day seven post immunization. ( A ) Histopathology shows few, randomly distributed foci of hepatocellular necrosis and loss with macrophage and lymphocyte infiltration (arrow). Furthermore, the hepatocytes display moderate, coalescing to diffuse, floccular cytoplasmic vacuolization, interpreted as a species-specific, relatively high level of glycogen storage. Hematoxylin-eosin. Bar = 100 μm; ( B ) Immunohistochemistry for Rift Valley fever phlebovirus (RVFV) Gc antigen reveals minor amounts of intra- and extracellular, strongly immunoreactive granula within the lesions (arrow), interpreted as debris remaining after virus-induced hepatocellular death. Immunohistochemistry, monoclonal mouse anti-RVFV Gc-protein antibody, avidin-biotin-peroxidase-complex method, 3-amino-9-ethyl-carbazol chromogen (red), hematoxylin counterstain (blue). Bar = 20 μm.

    Article Snippet: Immunohistology was performed using a mouse monoclonal antibody against the RVFV Gc-protein (clone: GC9A9) [ ], the avidin–biotin–peroxidase complex method (ABC, Elite PK6100; Vector Laboratories, Burlingame, CA, USA) with 3-amino-9-ethylcarbazole (AEC, Dako, Glostrup, Denmark) as chromogen and hematoxylin counterstain.

    Techniques: Histopathology, Immunohistochemistry, Avidin-Biotin Assay

    Immunohistochemical and subcellular localization of transgenic survivin expression. ( a ) Five-micrometer tissue sections were cut from fresh-frozen skin of K14-survivin transgenic mice (K14-survivin) or control nontransgenic (non-TG) littermates, fixed in ice-cold acetone, and analyzed by immunohistochemistry with Ab against survivin or a control Ab to EGFP. Binding of the primary Ab’s was visualized with a goat anti-rabbit polyclonal Ab using Vectastain Elite ABC and AEC peroxidase substrate kits. ( b ) Subcellular localization of transgenic survivin. Keratinocytes isolated from K14-survivin transgenic mice (K14-survivin) or nontransgenic littermates (non-TG) were adhered to glass coverslips, fixed in methanol-acetone, and incubated with an Ab to survivin, followed by Texas red–conjugated goat anti-rabbit Ab. Nuclei were stained with Hoechst 33342. Image merging analysis is shown.

    Journal: Journal of Clinical Investigation

    Article Title: Transgenic expression of survivin in keratinocytes counteracts UVB-induced apoptosis and cooperates with loss of p53

    doi:

    Figure Lengend Snippet: Immunohistochemical and subcellular localization of transgenic survivin expression. ( a ) Five-micrometer tissue sections were cut from fresh-frozen skin of K14-survivin transgenic mice (K14-survivin) or control nontransgenic (non-TG) littermates, fixed in ice-cold acetone, and analyzed by immunohistochemistry with Ab against survivin or a control Ab to EGFP. Binding of the primary Ab’s was visualized with a goat anti-rabbit polyclonal Ab using Vectastain Elite ABC and AEC peroxidase substrate kits. ( b ) Subcellular localization of transgenic survivin. Keratinocytes isolated from K14-survivin transgenic mice (K14-survivin) or nontransgenic littermates (non-TG) were adhered to glass coverslips, fixed in methanol-acetone, and incubated with an Ab to survivin, followed by Texas red–conjugated goat anti-rabbit Ab. Nuclei were stained with Hoechst 33342. Image merging analysis is shown.

    Article Snippet: Binding of the primary Ab was visualized using a goat anti-rabbit polyclonal Ab (1:100) and Vectastain Elite ABC and 3-amino-9-ethyl carbazole (AEC) peroxidase substrate kits (Vector Laboratories) according to the manufacturer’s instructions.

    Techniques: Immunohistochemistry, Transgenic Assay, Expressing, Mouse Assay, Binding Assay, Isolation, Incubation, Staining

    Detection of GNAQ in TG neurons during latency-reactivation cycle. TG were collected from 3 uninfected calves (U) (A), latently infected calves (L) (B), or latently infected calves treated with DEX for 6 h to initiate reactivation from latency (DEX) (C). Thin sections were cut from formalin-fixed, paraffin-embedded TG sections. The GNAQ antibody (ab75825; Abcam) was diluted 1:450. Biotinylated goat anti-rabbit IgG (Vector Laboratories) was used as a secondary antibody. Arrows denote GNAQ + neurons in the respective samples. (D) The percentage of GNAQ-positive neurons from 296 uninfected neurons, 241 latently infected neurons, and 209 TG neurons at 6 h after latently infected calves were treated with DEX. An asterisk denotes significant differences ( P

    Journal: Journal of Virology

    Article Title: The Wnt Signaling Pathway Is Differentially Expressed during the Bovine Herpesvirus 1 Latency-Reactivation Cycle: Evidence That Two Protein Kinases Associated with Neuronal Survival, Akt3 and BMPR2, Are Expressed at Higher Levels during Latency

    doi: 10.1128/JVI.01937-17

    Figure Lengend Snippet: Detection of GNAQ in TG neurons during latency-reactivation cycle. TG were collected from 3 uninfected calves (U) (A), latently infected calves (L) (B), or latently infected calves treated with DEX for 6 h to initiate reactivation from latency (DEX) (C). Thin sections were cut from formalin-fixed, paraffin-embedded TG sections. The GNAQ antibody (ab75825; Abcam) was diluted 1:450. Biotinylated goat anti-rabbit IgG (Vector Laboratories) was used as a secondary antibody. Arrows denote GNAQ + neurons in the respective samples. (D) The percentage of GNAQ-positive neurons from 296 uninfected neurons, 241 latently infected neurons, and 209 TG neurons at 6 h after latently infected calves were treated with DEX. An asterisk denotes significant differences ( P

    Article Snippet: The next day, slides were washed in 1× Tris-buffered saline (TBS) and incubated in biotinylated goat anti-rabbit IgG (PK-6101; Vector Laboratories) for 30 min at room temperature in a humidified chamber.

    Techniques: Infection, Formalin-fixed Paraffin-Embedded, Plasmid Preparation

    Akt3 is frequently detected in ORF2 + neurons during latency. Consecutive sections from formalin-fixed paraffin-embedded TG sections from latently infected calves were prepared. One section was stained with the Akt3 antibody (ab152157; Abcam) that was diluted 1:500. A consecutive section was stained with a peptide-specific ORF2 antibody (1:500 dilution). Biotinylated goat anti-rabbit IgG (Vector Laboratories) was used as a secondary antibody for the sections. Areas of sections that contained ORF2 + neurons were subsequently examined for Akt3 staining. Numbers denote the ORF2-positive neurons, and neurons 1, 3, 4, and 5 were also Akt3 + . Magnification is approximately 400×, and these sections are representative of many sections that were examined.

    Journal: Journal of Virology

    Article Title: The Wnt Signaling Pathway Is Differentially Expressed during the Bovine Herpesvirus 1 Latency-Reactivation Cycle: Evidence That Two Protein Kinases Associated with Neuronal Survival, Akt3 and BMPR2, Are Expressed at Higher Levels during Latency

    doi: 10.1128/JVI.01937-17

    Figure Lengend Snippet: Akt3 is frequently detected in ORF2 + neurons during latency. Consecutive sections from formalin-fixed paraffin-embedded TG sections from latently infected calves were prepared. One section was stained with the Akt3 antibody (ab152157; Abcam) that was diluted 1:500. A consecutive section was stained with a peptide-specific ORF2 antibody (1:500 dilution). Biotinylated goat anti-rabbit IgG (Vector Laboratories) was used as a secondary antibody for the sections. Areas of sections that contained ORF2 + neurons were subsequently examined for Akt3 staining. Numbers denote the ORF2-positive neurons, and neurons 1, 3, 4, and 5 were also Akt3 + . Magnification is approximately 400×, and these sections are representative of many sections that were examined.

    Article Snippet: The next day, slides were washed in 1× Tris-buffered saline (TBS) and incubated in biotinylated goat anti-rabbit IgG (PK-6101; Vector Laboratories) for 30 min at room temperature in a humidified chamber.

    Techniques: Formalin-fixed Paraffin-Embedded, Infection, Staining, Plasmid Preparation

    Comparison of Akt3 expression during the BoHV-1 latency-reactivation cycle. (A) TG were collected from 3 uninfected calves, 3 latently infected calves, or 3 latently infected calves treated with DEX for 6 h to initiate reactivation from latency. Thin sections were cut from formalin-fixed paraffin-embedded TG sections. The Akt3 antibody (ab152157; Abcam) was diluted 1:500. Biotinylated goat anti-rabbit IgG (Vector Laboratories) was used as a secondary antibody. Blue arrows denote Akt3-positive TG neurons that contained an Akt3-positive nucleus and a counterstained nucleolus. Black arrows denote TG neurons in which the nucleus was not visible, but they were Akt3 + . Closed circles denote TG neurons that contain a nucleus in which the nucleolus is counterstained but was not stained by the Akt3 antibody. These images are representative of many sections stained with the Akt3 antibody. Magnification is approximately 400×. (B) The percentage of Akt3-positive TG neurons from 500 total neurons was estimated from sections derived from 3 latently infected calves, 3 mock-infected calves, and 3 latently infected calves treated with DEX for 6 h. An asterisk denotes significant differences ( P

    Journal: Journal of Virology

    Article Title: The Wnt Signaling Pathway Is Differentially Expressed during the Bovine Herpesvirus 1 Latency-Reactivation Cycle: Evidence That Two Protein Kinases Associated with Neuronal Survival, Akt3 and BMPR2, Are Expressed at Higher Levels during Latency

    doi: 10.1128/JVI.01937-17

    Figure Lengend Snippet: Comparison of Akt3 expression during the BoHV-1 latency-reactivation cycle. (A) TG were collected from 3 uninfected calves, 3 latently infected calves, or 3 latently infected calves treated with DEX for 6 h to initiate reactivation from latency. Thin sections were cut from formalin-fixed paraffin-embedded TG sections. The Akt3 antibody (ab152157; Abcam) was diluted 1:500. Biotinylated goat anti-rabbit IgG (Vector Laboratories) was used as a secondary antibody. Blue arrows denote Akt3-positive TG neurons that contained an Akt3-positive nucleus and a counterstained nucleolus. Black arrows denote TG neurons in which the nucleus was not visible, but they were Akt3 + . Closed circles denote TG neurons that contain a nucleus in which the nucleolus is counterstained but was not stained by the Akt3 antibody. These images are representative of many sections stained with the Akt3 antibody. Magnification is approximately 400×. (B) The percentage of Akt3-positive TG neurons from 500 total neurons was estimated from sections derived from 3 latently infected calves, 3 mock-infected calves, and 3 latently infected calves treated with DEX for 6 h. An asterisk denotes significant differences ( P

    Article Snippet: The next day, slides were washed in 1× Tris-buffered saline (TBS) and incubated in biotinylated goat anti-rabbit IgG (PK-6101; Vector Laboratories) for 30 min at room temperature in a humidified chamber.

    Techniques: Expressing, Infection, Formalin-fixed Paraffin-Embedded, Plasmid Preparation, Staining, Derivative Assay