Journal: Molecules

Article Title: Synthesis and Biological Evaluation of Fangchinoline Derivatives as Anti-Inflammatory Agents through Inactivation of Inflammasome

doi: 10.3390/molecules24061154

Figure Lengend Snippet: ( A ) Immunoblot analysis of indicated proteins in culture supernatants (SN) and cell lysis (Input) of PMA primed THP-1 cells treated with target compounds (5 μM) and then stimulated with LPS (5 µg/mL) and NIG (20 µM). ( B ) Caspase 1 activity assay for purified caspase1 with or without presence of compounds 3h and 6 or positive control. ( C ) Immunoblot analysis of NF-κB and MAPK signaling pathway in 293 cells treated with TNF-α (20 ng/mL) with or without compounds 3h and 6 (5 μM). The protein levels were normalized against tublin.

Article Snippet: Anti-IL-1β, Anti-Caspase1, TNF-α were purchased from R&D Systems (Minneapolis, MN, USA); Anti-cleaved caspase1, Anti-NLRP3, Anti-IκBα, Anti-phospho-P38, Anti-phospho-ERK, Anti-phospho-JNK, Anti-phospho-IKKα/β, and Anti-Tublin were purchased from Cell Signaling Technology (Boston, MA, USA); Anti-TAK1, Anti-phospho-TAK1, Anti-ASC and recombinant active caspase1 protein were purchased from Abcam (Shanghai, China).

Techniques: Western Blot, Lysis, Activity Assay, Purification, Positive Control