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Thermo Fisher aari
Aari, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/aari/product/Thermo Fisher
Average 99 stars, based on 2 article reviews
Price from $9.99 to $1999.99
aari - by Bioz Stars, 2020-02
99/100 stars

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Related Articles

Clone Assay:

Article Title: BCL6 corepressor contributes to Th17 cell formation by inhibiting Th17 fate suppressors
Article Snippet: Then, the shRNA-encoding segment was replaced with cytotoxic protein CCDB bacterial gene flanked by SapI cut sites using In-Fusion cloning (Takara Bio). .. A golden gate reaction was performed to generate the tRNA–gRNA arrays ( ; ; ) using AarI (Thermo Fisher Scientific) and T4 DNA ligase (New England Biolabs).

Transfection:

Article Title: BCL6 corepressor contributes to Th17 cell formation by inhibiting Th17 fate suppressors
Article Snippet: A golden gate reaction was performed to generate the tRNA–gRNA arrays ( ; ; ) using AarI (Thermo Fisher Scientific) and T4 DNA ligase (New England Biolabs). .. Briefly, Platinum-E cells (Cell Biolabs) were plated in 6-well plates the evening before transfection.

shRNA:

Article Title: BCL6 corepressor contributes to Th17 cell formation by inhibiting Th17 fate suppressors
Article Snippet: Then, the shRNA-encoding segment was replaced with cytotoxic protein CCDB bacterial gene flanked by SapI cut sites using In-Fusion cloning (Takara Bio). .. A golden gate reaction was performed to generate the tRNA–gRNA arrays ( ; ; ) using AarI (Thermo Fisher Scientific) and T4 DNA ligase (New England Biolabs).

Purification:

Article Title: BCL6 corepressor contributes to Th17 cell formation by inhibiting Th17 fate suppressors
Article Snippet: A golden gate reaction was performed to generate the tRNA–gRNA arrays ( ; ; ) using AarI (Thermo Fisher Scientific) and T4 DNA ligase (New England Biolabs). .. The product was purified with a MinElute PCR Purification Kit (Qiagen), and a second golden gate reaction was performed using SapI (New England Biolabs) and T4 DNA ligase (New England Biolabs) to replace the CCDB gene in the pMCIA vector with the tRNA–gRNA arrays made from the first golden gate reaction.

Modification:

Article Title: BCL6 corepressor contributes to Th17 cell formation by inhibiting Th17 fate suppressors
Article Snippet: This vector involves modification of the LMP-Amt vector , a gift from S. Crotty (La Jolla Institute, La Jolla, CA). .. A golden gate reaction was performed to generate the tRNA–gRNA arrays ( ; ; ) using AarI (Thermo Fisher Scientific) and T4 DNA ligase (New England Biolabs).

Derivative Assay:

Article Title: BCL6 corepressor contributes to Th17 cell formation by inhibiting Th17 fate suppressors
Article Snippet: The CCDB gene derived from the pMOD_B2303 plasmid from D. Voytas (University of Minnesota, Minneapolis, MN). .. A golden gate reaction was performed to generate the tRNA–gRNA arrays ( ; ; ) using AarI (Thermo Fisher Scientific) and T4 DNA ligase (New England Biolabs).

CRISPR:

Article Title: BCL6 corepressor contributes to Th17 cell formation by inhibiting Th17 fate suppressors
Article Snippet: Paragraph title: CRISPR/Cas9 and retroviral transductions ... A golden gate reaction was performed to generate the tRNA–gRNA arrays ( ; ; ) using AarI (Thermo Fisher Scientific) and T4 DNA ligase (New England Biolabs).

Polymerase Chain Reaction:

Article Title: BCL6 corepressor contributes to Th17 cell formation by inhibiting Th17 fate suppressors
Article Snippet: A golden gate reaction was performed to generate the tRNA–gRNA arrays ( ; ; ) using AarI (Thermo Fisher Scientific) and T4 DNA ligase (New England Biolabs). .. The product was purified with a MinElute PCR Purification Kit (Qiagen), and a second golden gate reaction was performed using SapI (New England Biolabs) and T4 DNA ligase (New England Biolabs) to replace the CCDB gene in the pMCIA vector with the tRNA–gRNA arrays made from the first golden gate reaction.

Plasmid Preparation:

Article Title: BCL6 corepressor contributes to Th17 cell formation by inhibiting Th17 fate suppressors
Article Snippet: The PGK promoter present in the LMP-Amt vector was replaced with an internal ribosome entry site (plasmid 52109; Addgene) to generate the pMCIA vector. .. A golden gate reaction was performed to generate the tRNA–gRNA arrays ( ; ; ) using AarI (Thermo Fisher Scientific) and T4 DNA ligase (New England Biolabs).

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    Thermo Fisher aari
    <t>pKIR1.1</t> construction. (A) For pKIR1.1, U6.26p::2 × <t>AarI:sgRNA</t> was inserted into the Sbf I site of pKIR1.0. (B) Flowchart of pKIR1.1-based vector construction. The upper sequence is an enlarged view of the boundary (yellow) between the U6.26 promoter (pale green) and the sgRNA scaffold (orange). This boundary contains an inverted repeat of Aar I recognition sites, and Aar I digestion generates four-base overhangs. A hybridized primer with overhangs can be inserted into this site as shown. G with a red triangle is the first base for RNA transcription from the U6.26 promoter. G + (N) 19 indicates the target sequence.
    Aari, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aari/product/Thermo Fisher
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    aari - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    82
    Thermo Fisher oligos thermocycler aari enzyme
    Insertion of gRNA sequences into AAV vector gRNAs are inserted into AAV plasmid containing U6 promoters and cardiac troponin T (cTNT) promoter-driven Cre recombinase. Annealed <t>oligos</t> corresponding to the targeting portions of gRNA1 and gRNA2 are cloned between <t>AarI</t> and SapI sites, respectively. The entire construct is contained within AAV inverted terminal repeat sequences, allowing for packaging into AAV capsids. Examples of gRNAs designed against Gata4 are shown.
    Oligos Thermocycler Aari Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligos thermocycler aari enzyme/product/Thermo Fisher
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    oligos thermocycler aari enzyme - by Bioz Stars, 2020-02
    82/100 stars
      Buy from Supplier

    N/A
    Thermo Scientific 10X Buffer AarI AjiI Bpu10I ScaI PasI is the optimal buffer recommended for use with AarI AjiI Bpu10I ScaI and PasI restriction enzymes and is premixed with BSA
      Buy from Supplier

    Image Search Results


    pKIR1.1 construction. (A) For pKIR1.1, U6.26p::2 × AarI:sgRNA was inserted into the Sbf I site of pKIR1.0. (B) Flowchart of pKIR1.1-based vector construction. The upper sequence is an enlarged view of the boundary (yellow) between the U6.26 promoter (pale green) and the sgRNA scaffold (orange). This boundary contains an inverted repeat of Aar I recognition sites, and Aar I digestion generates four-base overhangs. A hybridized primer with overhangs can be inserted into this site as shown. G with a red triangle is the first base for RNA transcription from the U6.26 promoter. G + (N) 19 indicates the target sequence.

    Journal: Plant and Cell Physiology

    Article Title: pKAMA-ITACHI Vectors for Highly Efficient CRISPR/Cas9-Mediated Gene Knockout in Arabidopsis thaliana

    doi: 10.1093/pcp/pcw191

    Figure Lengend Snippet: pKIR1.1 construction. (A) For pKIR1.1, U6.26p::2 × AarI:sgRNA was inserted into the Sbf I site of pKIR1.0. (B) Flowchart of pKIR1.1-based vector construction. The upper sequence is an enlarged view of the boundary (yellow) between the U6.26 promoter (pale green) and the sgRNA scaffold (orange). This boundary contains an inverted repeat of Aar I recognition sites, and Aar I digestion generates four-base overhangs. A hybridized primer with overhangs can be inserted into this site as shown. G with a red triangle is the first base for RNA transcription from the U6.26 promoter. G + (N) 19 indicates the target sequence.

    Article Snippet: For pKIR1.1_PSD3, pKIR1.1_ADH1-2 and pKI1.1R_PDS3, pKIR1.1 or pKI1.1R was digested by Aar I (Thermo Fisher Scientific, #ER1581) with alkaline phosphatase rAPid (Roche, #04898133001).

    Techniques: Plasmid Preparation, Sequencing

    Primer design for introducing point mutations using AarI and SapI. (A) Recognition sites and cutting patterns for the SapI and AarI restriction enzymes. (B) Hypothetical sequence that will be targeted for mutagenesis. (C) Primers that can be used to mutate the sequence shown in (B) with the SIMPLE method, using the SapI enzyme.

    Journal: Journal of molecular biology

    Article Title: Development of the SapI/AarI Incision Mediated Plasmid Editing Method

    doi: 10.1016/j.jmb.2018.03.030

    Figure Lengend Snippet: Primer design for introducing point mutations using AarI and SapI. (A) Recognition sites and cutting patterns for the SapI and AarI restriction enzymes. (B) Hypothetical sequence that will be targeted for mutagenesis. (C) Primers that can be used to mutate the sequence shown in (B) with the SIMPLE method, using the SapI enzyme.

    Article Snippet: After amplification, PCR products were first digested with DpnI (NEB) to eliminate the parental plasmid, cleaned up over columns (Qiagen), and then digested with either the SapI (NEB) or AarI (Thermo) restriction enzyme, following the manufacturer’s specifications.

    Techniques: Sequencing, Mutagenesis

    Insertion of gRNA sequences into AAV vector gRNAs are inserted into AAV plasmid containing U6 promoters and cardiac troponin T (cTNT) promoter-driven Cre recombinase. Annealed oligos corresponding to the targeting portions of gRNA1 and gRNA2 are cloned between AarI and SapI sites, respectively. The entire construct is contained within AAV inverted terminal repeat sequences, allowing for packaging into AAV capsids. Examples of gRNAs designed against Gata4 are shown.

    Journal: Current protocols in molecular biology

    Article Title: CASAAV: a CRISPR based platform for rapid dissection of gene function in vivo

    doi: 10.1002/cpmb.46

    Figure Lengend Snippet: Insertion of gRNA sequences into AAV vector gRNAs are inserted into AAV plasmid containing U6 promoters and cardiac troponin T (cTNT) promoter-driven Cre recombinase. Annealed oligos corresponding to the targeting portions of gRNA1 and gRNA2 are cloned between AarI and SapI sites, respectively. The entire construct is contained within AAV inverted terminal repeat sequences, allowing for packaging into AAV capsids. Examples of gRNAs designed against Gata4 are shown.

    Article Snippet: AAV-U6gRNA1-U6gRNA2-TnT-Cre Vector (Addgene #87682) gRNA sequences from Basic Protocol 1/DNA oligos Thermocycler AarI enzyme and buffer (ThermoFisher ER1581) SapI enzyme and buffer (NEB R0569L) 37°C incubator 37°C shaking incubator Agarose (GeneMate E-3120-500) 50× TAE (Boston BioProducts BM250) Ethidium bromide (Sigma E7637) DNA loading dye (NEB B7024S) Gel electrophoresis chamber UV transilluminator (365 nm) Gel purification kit (Invitrogen K210012) Quick ligation kit (NEB M2200L) Chemically competent cells such as ThermoFisher MAX Efficiency Stbl2 Competent Cells (Cat# 10268019).

    Techniques: Plasmid Preparation, Clone Assay, Construct

    Insertion of gRNA sequences into AAV vector gRNAs are inserted into AAV plasmid containing U6 promoters and cardiac troponin T (cTNT) promoter-driven Cre recombinase. Annealed oligos corresponding to the targeting portions of gRNA1 and gRNA2 are cloned between AarI and SapI sites, respectively. The entire construct is contained within AAV inverted terminal repeat sequences, allowing for packaging into AAV capsids. Examples of gRNAs designed against Gata4 are shown.

    Journal: Current protocols in molecular biology

    Article Title: CASAAV: a CRISPR based platform for rapid dissection of gene function in vivo

    doi: 10.1002/cpmb.46

    Figure Lengend Snippet: Insertion of gRNA sequences into AAV vector gRNAs are inserted into AAV plasmid containing U6 promoters and cardiac troponin T (cTNT) promoter-driven Cre recombinase. Annealed oligos corresponding to the targeting portions of gRNA1 and gRNA2 are cloned between AarI and SapI sites, respectively. The entire construct is contained within AAV inverted terminal repeat sequences, allowing for packaging into AAV capsids. Examples of gRNAs designed against Gata4 are shown.

    Article Snippet: AAV-U6gRNA1-U6gRNA2-TnT-Cre Vector (Addgene #87682) gRNA sequences from Basic Protocol 1/DNA oligos Thermocycler AarI enzyme and buffer (ThermoFisher ER1581) SapI enzyme and buffer (NEB R0569L) 37°C incubator 37°C shaking incubator Agarose (GeneMate E-3120-500) 50× TAE (Boston BioProducts BM250) Ethidium bromide (Sigma E7637) DNA loading dye (NEB B7024S) Gel electrophoresis chamber UV transilluminator (365 nm) Gel purification kit (Invitrogen K210012) Quick ligation kit (NEB M2200L) Chemically competent cells such as ThermoFisher MAX Efficiency Stbl2 Competent Cells (Cat# 10268019).

    Techniques: Plasmid Preparation, Clone Assay, Construct