human a549 lung epithelial cancer cell lines  (ATCC)

Journal: Antibiotics

Article Title: Broad-Spectrum Antibacterial Activity of Antioxidant Octyl Gallate and Its Impact on Gut Microbiome

doi: 10.3390/antibiotics13080731

Figure Lengend Snippet: Effect of octyl gallate (OG) on human cell viability and hemolytic activity in sheep red blood cells. Human normal fibroblast HFF cells ( A ) and human lung cancer epithelial A549 cells ( B ) were exposed to various concentrations of OG. Negative control cells were treated with DMSO as a vehicle control. Cell viability was measured after 24 h of treatment using a BioTek Synergy II spectrophotometer, as described in . The symbols ** and *** indicates a significant difference between the control and treated cells at p < 0.01 and p < 0.001, respectively. No significant difference is indicated as “ns”. Hemolytic activity of OG was assessed in sheep red blood cells ( C ), with standard saponin serving as the positive control and PBS as the negative control. Hemolysis (%) was calculated using the following formular: [(A450 test sample − A450 negative control)/(A450 positive control − A450 negative control)] × 100. Data represent the average of three samples per condition in a single experiment. Each experiment was repeated at least three times.

Article Snippet: Human foreskin fibroblast (HFF) cells (kindly provided by Dr. Roberta O’Connor) and human lung cancer epithelial A549 cells (ATCC) were cultured in RPMI 1640 medium and DMEM supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA, USA), respectively, as described [ ].

Techniques: Activity Assay, Negative Control, Control, Spectrophotometry, Positive Control