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Viromed a549 cells
A549 Cells, supplied by Viromed, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/a549 cells/product/Viromed
Average 92 stars, based on 1 article reviews
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a549 cells - by Bioz Stars, 2020-09
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Article Title: Real-Time PCR for detection of herpes simplex virus without nucleic acid extraction
Article Snippet: Aliquots (1 ml) of all prospective and retrospective specimens were combined with A549 cells (Viromed Laboratories, Minnetonka, MN) in shell vials and placed at 37°C.

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    Viromed cell culture a549
    High-binders induce prolonged degradation of IκB compared to low-binders. All values are mean ± one standard deviation, based on three independent experiments. 2A. IκB degradation induced by serotype 3 evolved high-binder and 3 low-binding parent strains. Closed squares, IκB level relative to time zero in <t>A549</t> treated with low-binder. Open triangles, IκB level relative to time zero in A549 treated with high-binder. Representative Western blots for IκB are shown below each line graph. 2B. IκB degradation induced by serotype 9N low-binding clinical isolate and serotype 6B high-binding clinical isolate. Closed squares, IκB level relative to time zero in A549 treated with low-binder. Open triangles, IκB level relative to time zero in A549 treated with high-binder. Representative Western blots for IκB shown below line graph.
    Cell Culture A549, supplied by Viromed, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell culture a549/product/Viromed
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cell culture a549 - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    85
    Viromed a549 cells dhi
    High-binders induce prolonged degradation of IκB compared to low-binders. All values are mean ± one standard deviation, based on three independent experiments. 2A. IκB degradation induced by serotype 3 evolved high-binder and 3 low-binding parent strains. Closed squares, IκB level relative to time zero in <t>A549</t> treated with low-binder. Open triangles, IκB level relative to time zero in A549 treated with high-binder. Representative Western blots for IκB are shown below each line graph. 2B. IκB degradation induced by serotype 9N low-binding clinical isolate and serotype 6B high-binding clinical isolate. Closed squares, IκB level relative to time zero in A549 treated with low-binder. Open triangles, IκB level relative to time zero in A549 treated with high-binder. Representative Western blots for IκB shown below line graph.
    A549 Cells Dhi, supplied by Viromed, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a549 cells dhi/product/Viromed
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a549 cells dhi - by Bioz Stars, 2020-09
    85/100 stars
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    85
    Viromed human epidermoid tumor a549 cells
    High-binders induce prolonged degradation of IκB compared to low-binders. All values are mean ± one standard deviation, based on three independent experiments. 2A. IκB degradation induced by serotype 3 evolved high-binder and 3 low-binding parent strains. Closed squares, IκB level relative to time zero in <t>A549</t> treated with low-binder. Open triangles, IκB level relative to time zero in A549 treated with high-binder. Representative Western blots for IκB are shown below each line graph. 2B. IκB degradation induced by serotype 9N low-binding clinical isolate and serotype 6B high-binding clinical isolate. Closed squares, IκB level relative to time zero in A549 treated with low-binder. Open triangles, IκB level relative to time zero in A549 treated with high-binder. Representative Western blots for IκB shown below line graph.
    Human Epidermoid Tumor A549 Cells, supplied by Viromed, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human epidermoid tumor a549 cells/product/Viromed
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human epidermoid tumor a549 cells - by Bioz Stars, 2020-09
    85/100 stars
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    80
    Viromed epitheloid cell lines
    High-binders induce prolonged degradation of IκB compared to low-binders. All values are mean ± one standard deviation, based on three independent experiments. 2A. IκB degradation induced by serotype 3 evolved high-binder and 3 low-binding parent strains. Closed squares, IκB level relative to time zero in <t>A549</t> treated with low-binder. Open triangles, IκB level relative to time zero in A549 treated with high-binder. Representative Western blots for IκB are shown below each line graph. 2B. IκB degradation induced by serotype 9N low-binding clinical isolate and serotype 6B high-binding clinical isolate. Closed squares, IκB level relative to time zero in A549 treated with low-binder. Open triangles, IκB level relative to time zero in A549 treated with high-binder. Representative Western blots for IκB shown below line graph.
    Epitheloid Cell Lines, supplied by Viromed, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    epitheloid cell lines - by Bioz Stars, 2020-09
    80/100 stars
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    High-binders induce prolonged degradation of IκB compared to low-binders. All values are mean ± one standard deviation, based on three independent experiments. 2A. IκB degradation induced by serotype 3 evolved high-binder and 3 low-binding parent strains. Closed squares, IκB level relative to time zero in A549 treated with low-binder. Open triangles, IκB level relative to time zero in A549 treated with high-binder. Representative Western blots for IκB are shown below each line graph. 2B. IκB degradation induced by serotype 9N low-binding clinical isolate and serotype 6B high-binding clinical isolate. Closed squares, IκB level relative to time zero in A549 treated with low-binder. Open triangles, IκB level relative to time zero in A549 treated with high-binder. Representative Western blots for IκB shown below line graph.

    Journal: BMC Infectious Diseases

    Article Title: Differential activation of inflammatory pathways in A549 type II pneumocytes by Streptococcus pneumoniae strains with different adherence properties

    doi: 10.1186/1471-2334-6-71

    Figure Lengend Snippet: High-binders induce prolonged degradation of IκB compared to low-binders. All values are mean ± one standard deviation, based on three independent experiments. 2A. IκB degradation induced by serotype 3 evolved high-binder and 3 low-binding parent strains. Closed squares, IκB level relative to time zero in A549 treated with low-binder. Open triangles, IκB level relative to time zero in A549 treated with high-binder. Representative Western blots for IκB are shown below each line graph. 2B. IκB degradation induced by serotype 9N low-binding clinical isolate and serotype 6B high-binding clinical isolate. Closed squares, IκB level relative to time zero in A549 treated with low-binder. Open triangles, IκB level relative to time zero in A549 treated with high-binder. Representative Western blots for IκB shown below line graph.

    Article Snippet: Cell culture A549, a human pneumocyte II cell line, was obtained from ViroMed.

    Techniques: Standard Deviation, Binding Assay, Western Blot

    Adherence of high-binders, but not low-binders, is reduced by blocking CbpA or C3. Solid bars, negative control; open bars, bacteria pre-treated with anti-CbpA antibody; dotted bars, A549 cells pre-treated with anti-C3 antibody. Negative control A549 cells pre-treated with normal rabbit serum. All values are mean ± one standard deviation, based on three independent experiments. Mean values are above each bar. The Y-axis units differ on graphs 1A and 1B. 1A. High-binding S. pneumoniae are identified by serotype along X-axis. 6301 evolved, 3 evolved, and 18C evolved are high-binding descendants of low-binding parent strains. Isolates identifed as 6 B all share that serotype but have different PFGE patterns. 1B. Low-binding S. pneumoniae strains are identified by serotype along X-axis. 6301 parent, 3 parent, and 18C parent are initial low-binding strains of 6301 evolved, 3 evolved, and 18C evolved.

    Journal: BMC Infectious Diseases

    Article Title: Differential activation of inflammatory pathways in A549 type II pneumocytes by Streptococcus pneumoniae strains with different adherence properties

    doi: 10.1186/1471-2334-6-71

    Figure Lengend Snippet: Adherence of high-binders, but not low-binders, is reduced by blocking CbpA or C3. Solid bars, negative control; open bars, bacteria pre-treated with anti-CbpA antibody; dotted bars, A549 cells pre-treated with anti-C3 antibody. Negative control A549 cells pre-treated with normal rabbit serum. All values are mean ± one standard deviation, based on three independent experiments. Mean values are above each bar. The Y-axis units differ on graphs 1A and 1B. 1A. High-binding S. pneumoniae are identified by serotype along X-axis. 6301 evolved, 3 evolved, and 18C evolved are high-binding descendants of low-binding parent strains. Isolates identifed as 6 B all share that serotype but have different PFGE patterns. 1B. Low-binding S. pneumoniae strains are identified by serotype along X-axis. 6301 parent, 3 parent, and 18C parent are initial low-binding strains of 6301 evolved, 3 evolved, and 18C evolved.

    Article Snippet: Cell culture A549, a human pneumocyte II cell line, was obtained from ViroMed.

    Techniques: Blocking Assay, Negative Control, Standard Deviation, Binding Assay

    NFκB migrates to the nucleus after cell interaction with high-binders, but not low-binders, 4 hours after inoculation with bacteria. NFκB is detected as a dark brown stain against a pale lavender background. 3A, negative control. 3B, A549 incubated with serotype 3 low-binders (Black arrow shows NFκB protein in the cytoplasm). 3C, A549 incubated with serotype 3 high-binders (White arrow shows NFκB protein detected in the nucleus).

    Journal: BMC Infectious Diseases

    Article Title: Differential activation of inflammatory pathways in A549 type II pneumocytes by Streptococcus pneumoniae strains with different adherence properties

    doi: 10.1186/1471-2334-6-71

    Figure Lengend Snippet: NFκB migrates to the nucleus after cell interaction with high-binders, but not low-binders, 4 hours after inoculation with bacteria. NFκB is detected as a dark brown stain against a pale lavender background. 3A, negative control. 3B, A549 incubated with serotype 3 low-binders (Black arrow shows NFκB protein in the cytoplasm). 3C, A549 incubated with serotype 3 high-binders (White arrow shows NFκB protein detected in the nucleus).

    Article Snippet: Cell culture A549, a human pneumocyte II cell line, was obtained from ViroMed.

    Techniques: Staining, Negative Control, Incubation

    Supernatants from A549 cells treated with high- and low-binding S. pneumoniae were evaluated with a RayBio ® Human Cytokine Antibody Array III, which detects 42 different human cytokines. 1A and 2A, positive control; 3A and 4A, negative control; 5A, ENA-78; 6A, GCSF; 7A, GM-CSF, 8A, GRO; 9A, Gro-α; 10A, I-309; 11A, IL-1α; 12A, IL-1β; 1B, IL-2; 2B, IL-3; 3B, IL-4; 4B, IL-5; 5B, IL-6; 6B, IL-7; 7B, IL-8; 8B, IL-10; 9B, IL-12; 10B, IL-13; 11B, IL-15; 12B, IFN-γ; 1C, MCP-1; 2C, MCP-2; 3C, MCP-3; 4C, MCSF; 5C, MDC; 6C, MIG; 7C, MIP-1; 8C, RANTES; 9C, SCF; 10C, SDF-1; 11C, TARC; 12C, TGF-β; 1D, TNF-α; 2D, TNF-β; 3D, EGF; 4D, IGF-1; 5D, angiogenin; 6D, oncostatin M; 7D, thrombopoietin; 8D, VEGF; 9D, PDGF; 10D, leptin; 11D, negative control; 12D, positive control. The positive result for IL-8, in position 7B, with negative results for all other cytokines, was observed for A549 cells treated with both high- and low-binding bacteria. Experiment replicated with four different high-binding, and four different low-binding, S. pneumoniae strains.

    Journal: BMC Infectious Diseases

    Article Title: Differential activation of inflammatory pathways in A549 type II pneumocytes by Streptococcus pneumoniae strains with different adherence properties

    doi: 10.1186/1471-2334-6-71

    Figure Lengend Snippet: Supernatants from A549 cells treated with high- and low-binding S. pneumoniae were evaluated with a RayBio ® Human Cytokine Antibody Array III, which detects 42 different human cytokines. 1A and 2A, positive control; 3A and 4A, negative control; 5A, ENA-78; 6A, GCSF; 7A, GM-CSF, 8A, GRO; 9A, Gro-α; 10A, I-309; 11A, IL-1α; 12A, IL-1β; 1B, IL-2; 2B, IL-3; 3B, IL-4; 4B, IL-5; 5B, IL-6; 6B, IL-7; 7B, IL-8; 8B, IL-10; 9B, IL-12; 10B, IL-13; 11B, IL-15; 12B, IFN-γ; 1C, MCP-1; 2C, MCP-2; 3C, MCP-3; 4C, MCSF; 5C, MDC; 6C, MIG; 7C, MIP-1; 8C, RANTES; 9C, SCF; 10C, SDF-1; 11C, TARC; 12C, TGF-β; 1D, TNF-α; 2D, TNF-β; 3D, EGF; 4D, IGF-1; 5D, angiogenin; 6D, oncostatin M; 7D, thrombopoietin; 8D, VEGF; 9D, PDGF; 10D, leptin; 11D, negative control; 12D, positive control. The positive result for IL-8, in position 7B, with negative results for all other cytokines, was observed for A549 cells treated with both high- and low-binding bacteria. Experiment replicated with four different high-binding, and four different low-binding, S. pneumoniae strains.

    Article Snippet: Cell culture A549, a human pneumocyte II cell line, was obtained from ViroMed.

    Techniques: Binding Assay, Ab Array, Positive Control, Negative Control