a549 cells  (Novus Biologicals)

 
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    Name:
    A549 Cell Lysate
    Description:

    Catalog Number:
    nbp2-25049
    Price:
    199
    Immunogen:
    A549 cell line lysate (lung carcinoma)
    Size:
    0 1 mg
    Category:
    Lysates
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    Structured Review

    Novus Biologicals a549 cells
    NEU3 surface expression on airway ECs. Flow cytometry of surface NEU3 on ( A ) 1HAEo − , ( B ) CFTE29o − , ( C ) 16HBE14o − , ( D ) BEAS-2B, ( E ) SAEC, and ( F ) <t>A549</t> airway ECs. The gray-shaded histograms represent staining with control nonimmune

    https://www.bioz.com/result/a549 cells/product/Novus Biologicals
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a549 cells - by Bioz Stars, 2021-01
    94/100 stars

    Images

    1) Product Images from "Human airway epithelia express catalytically active NEU3 sialidase"

    Article Title: Human airway epithelia express catalytically active NEU3 sialidase

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    doi: 10.1152/ajplung.00322.2013

    NEU3 surface expression on airway ECs. Flow cytometry of surface NEU3 on ( A ) 1HAEo − , ( B ) CFTE29o − , ( C ) 16HBE14o − , ( D ) BEAS-2B, ( E ) SAEC, and ( F ) A549 airway ECs. The gray-shaded histograms represent staining with control nonimmune
    Figure Legend Snippet: NEU3 surface expression on airway ECs. Flow cytometry of surface NEU3 on ( A ) 1HAEo − , ( B ) CFTE29o − , ( C ) 16HBE14o − , ( D ) BEAS-2B, ( E ) SAEC, and ( F ) A549 airway ECs. The gray-shaded histograms represent staining with control nonimmune

    Techniques Used: Expressing, Flow Cytometry, Cytometry, Staining

    Airway epithelial cell (EC) sialidase activity. A : increasing primary small airway EC (SAEC; n = 2) and A549 ( n = 3) cell numbers were assayed for sialidase activity for a ganglioside mixture. B : A549 cells (1.0 × 10 6 ) were assayed for sialidase
    Figure Legend Snippet: Airway epithelial cell (EC) sialidase activity. A : increasing primary small airway EC (SAEC; n = 2) and A549 ( n = 3) cell numbers were assayed for sialidase activity for a ganglioside mixture. B : A549 cells (1.0 × 10 6 ) were assayed for sialidase

    Techniques Used: Activity Assay

    NEU3 catalytic activity in airway ECs. A–C : A549 cells were infected with increasing multiplicities of infection (MOIs) of Ad-NEU3-HA and cultured for 48 h. A : cells were lysed and the lysates were processed for immunoblotting with anti-hemagglutinin
    Figure Legend Snippet: NEU3 catalytic activity in airway ECs. A–C : A549 cells were infected with increasing multiplicities of infection (MOIs) of Ad-NEU3-HA and cultured for 48 h. A : cells were lysed and the lysates were processed for immunoblotting with anti-hemagglutinin

    Techniques Used: Activity Assay, Infection, Cell Culture

    Subcellular localization of NEU3. A : subconfluent A549 cells were probed with anti-NEU3 antibody ( I and ii ), or nonimmune IgG as a negative control ( iii ), and counterstained with DAPI (overlays in i and iii ). Arrows indicate the nuclear regions in NEU3-stained
    Figure Legend Snippet: Subcellular localization of NEU3. A : subconfluent A549 cells were probed with anti-NEU3 antibody ( I and ii ), or nonimmune IgG as a negative control ( iii ), and counterstained with DAPI (overlays in i and iii ). Arrows indicate the nuclear regions in NEU3-stained

    Techniques Used: Negative Control, Staining

    2) Product Images from "High-throughput single-EV liquid biopsy: Rapid, simultaneous, and multiplexed detection of nucleic acids, proteins, and their combinations"

    Article Title: High-throughput single-EV liquid biopsy: Rapid, simultaneous, and multiplexed detection of nucleic acids, proteins, and their combinations

    Journal: Science Advances

    doi: 10.1126/sciadv.abc1204

    Verification of PD-L1 protein expression on EVs. ( A ) Fluorescence signals of PBS only (no EVs and no antibodies), EV only (EVs without staining with any antibodies), EV isotype (EVs stained with isotype control antibody), and EV CD63 antibodies (Abs) (EVs stained with CD63 antibody). ( B ) Fluorescence signals of PBS only, EV only, EV isotype, and EV PD-L1 Abs (EVs stained with PD-L1 antibody). ( C ) Particle size and zeta potential measurement of the EVs isolated from A549 cells overexpressed with CD63-GFP by nanoparticle tracking analysis technique. d.nm, diameter (nm). ( D ) EVs isolated from human A549 cells overexpressed with CD63-GFP or PD-L1–mCherry ( E ) and diluted to different concentrations. 1:1, initial concentration; 1:10, 10-fold dilution; 1:100, 100-fold dilution; 1:1000, 1000-fold dilution.
    Figure Legend Snippet: Verification of PD-L1 protein expression on EVs. ( A ) Fluorescence signals of PBS only (no EVs and no antibodies), EV only (EVs without staining with any antibodies), EV isotype (EVs stained with isotype control antibody), and EV CD63 antibodies (Abs) (EVs stained with CD63 antibody). ( B ) Fluorescence signals of PBS only, EV only, EV isotype, and EV PD-L1 Abs (EVs stained with PD-L1 antibody). ( C ) Particle size and zeta potential measurement of the EVs isolated from A549 cells overexpressed with CD63-GFP by nanoparticle tracking analysis technique. d.nm, diameter (nm). ( D ) EVs isolated from human A549 cells overexpressed with CD63-GFP or PD-L1–mCherry ( E ) and diluted to different concentrations. 1:1, initial concentration; 1:10, 10-fold dilution; 1:100, 100-fold dilution; 1:1000, 1000-fold dilution.

    Techniques Used: Expressing, Fluorescence, Staining, Isolation, Concentration Assay

    Double immunofluorescence staining of CD63 and PD-L1 membrane protein. ( A ) EVs isolated from 1000 μl of cell supernatant of human A549 cells overexpressed with CD63-GFP and PD-L1–mCherry. The dots in the colocalized only image represent EVs expressing both CD63 and PD-L1 protein. ( B ) EVs isolated from 90 μl of human plasma are stained with CD63-AF488 and PD-L1–AF647 antibodies. The dots in the colocalized only image represent EVs expressing both CD63 and PD-L1 protein. ( C ) Ratio of CD63- and PD-L1–positive EVs to CD63-positive EVs isolated from human A549 cells overexpressed with CD63-GFP and PD-L1–mCherry or isolated from plasma of 20 patients with LUAD.
    Figure Legend Snippet: Double immunofluorescence staining of CD63 and PD-L1 membrane protein. ( A ) EVs isolated from 1000 μl of cell supernatant of human A549 cells overexpressed with CD63-GFP and PD-L1–mCherry. The dots in the colocalized only image represent EVs expressing both CD63 and PD-L1 protein. ( B ) EVs isolated from 90 μl of human plasma are stained with CD63-AF488 and PD-L1–AF647 antibodies. The dots in the colocalized only image represent EVs expressing both CD63 and PD-L1 protein. ( C ) Ratio of CD63- and PD-L1–positive EVs to CD63-positive EVs isolated from human A549 cells overexpressed with CD63-GFP and PD-L1–mCherry or isolated from plasma of 20 patients with LUAD.

    Techniques Used: Double Immunofluorescence Staining, Isolation, Expressing, Staining

    Measurement of EV mRNA and protein expression in vitro. ( A ) RT-qPCR and ( B ) Western blotting analysis of PD-L1 expression of vector and PD-L1–overexpressed A549 cells. ( C ) HNCIB analysis of PD-L1 mRNA and protein expression of EVs isolated from supernatant of A549 cells overexpressing vector and PD-L1 ( D ). ** P
    Figure Legend Snippet: Measurement of EV mRNA and protein expression in vitro. ( A ) RT-qPCR and ( B ) Western blotting analysis of PD-L1 expression of vector and PD-L1–overexpressed A549 cells. ( C ) HNCIB analysis of PD-L1 mRNA and protein expression of EVs isolated from supernatant of A549 cells overexpressing vector and PD-L1 ( D ). ** P

    Techniques Used: Expressing, In Vitro, Quantitative RT-PCR, Western Blot, Plasmid Preparation, Isolation

    3) Product Images from "Noninvasive Imaging and Quantification of Radiotherapy-Induced PD-L1 Upregulation with 89Zr–Df–Atezolizumab"

    Article Title: Noninvasive Imaging and Quantification of Radiotherapy-Induced PD-L1 Upregulation with 89Zr–Df–Atezolizumab

    Journal: Bioconjugate chemistry

    doi: 10.1021/acs.bioconjchem.9b00178

    A549 PD-L1 PET. PET imaging of mice with PD-L1(−) A549 xenografts with 89 Zr–Df–atezolizumab. Tumors are indicated by red dashed circles in the representative MIPs.
    Figure Legend Snippet: A549 PD-L1 PET. PET imaging of mice with PD-L1(−) A549 xenografts with 89 Zr–Df–atezolizumab. Tumors are indicated by red dashed circles in the representative MIPs.

    Techniques Used: Positron Emission Tomography, Imaging, Mouse Assay

    Quantification of tracer biodistribution. (A) Uptake of 89 Zr–Df–atezolizumab in H460 tumors as determined through region-of-interest analysis of PET images. (B) Tumor-to-muscle ratios for all studied H460 groups over time. Both analyses indicate the highest accumulation of 89 Zr–Df–atezolizumab in the fractionated radiotherapy group. (C) Tumor uptake over time in H460 and A549 tumor xenografts receiving 2 Gy × 5 Fx. (D) Ex vivo biodistribution study results in the two tumor models at 96 h postinjection of tracers. * p
    Figure Legend Snippet: Quantification of tracer biodistribution. (A) Uptake of 89 Zr–Df–atezolizumab in H460 tumors as determined through region-of-interest analysis of PET images. (B) Tumor-to-muscle ratios for all studied H460 groups over time. Both analyses indicate the highest accumulation of 89 Zr–Df–atezolizumab in the fractionated radiotherapy group. (C) Tumor uptake over time in H460 and A549 tumor xenografts receiving 2 Gy × 5 Fx. (D) Ex vivo biodistribution study results in the two tumor models at 96 h postinjection of tracers. * p

    Techniques Used: Positron Emission Tomography, Ex Vivo

    A549 tumor staining. Minimal PD-L1 staining was observed in all groups of A549 tumor-bearing mice. Scale bar = 20 μ m.
    Figure Legend Snippet: A549 tumor staining. Minimal PD-L1 staining was observed in all groups of A549 tumor-bearing mice. Scale bar = 20 μ m.

    Techniques Used: Staining, Mouse Assay

    4) Product Images from "Optimization of the Enrichment of Circulating Tumor Cells for Downstream Phenotypic Analysis in Patients with Non-Small Cell Lung Cancer Treated with Anti-PD-1 Immunotherapy"

    Article Title: Optimization of the Enrichment of Circulating Tumor Cells for Downstream Phenotypic Analysis in Patients with Non-Small Cell Lung Cancer Treated with Anti-PD-1 Immunotherapy

    Journal: Cancers

    doi: 10.3390/cancers12061556

    Comparison of manual circulating tumor cell (CTC)-enrichment approaches in spiking experiments: ( A ) Recovery rates of pre-labeled A549, H1975, and SKMES-1 cells following enrichment with each method. Values represent mean recoveries; error bars represent standard error of mean (SEM). * , ** One-way ANOVA test; statistical significance at the p
    Figure Legend Snippet: Comparison of manual circulating tumor cell (CTC)-enrichment approaches in spiking experiments: ( A ) Recovery rates of pre-labeled A549, H1975, and SKMES-1 cells following enrichment with each method. Values represent mean recoveries; error bars represent standard error of mean (SEM). * , ** One-way ANOVA test; statistical significance at the p

    Techniques Used: Labeling

    Optimization of cell harvest on slides for downstream protein expression analysis: ( Ai ) Recovery of pre-labeled H1975 and A549 spiked cells transferred on lysine-non-coated and lysine-coated SuperFrost Plus™ adhesion slides. ( Aii ) Immunofluorescence staining among lysine-non-coated and lysine-coated slides; representative staining of leukocyte common antigen (CD45)/DAPI on peripheral blood mononuclear cells (PBMCs) and of cytokeratins (CK)/DAPI on non-pre-labeled A549 cells (Ariol microscopy system, Genetix, New Milton, UK) (400×). ( Bi ) Recovery of pre-labeled cells using different cell harvest approaches. * , ** One-way analysis of variance (ANOVA) test; statistical significance at the p
    Figure Legend Snippet: Optimization of cell harvest on slides for downstream protein expression analysis: ( Ai ) Recovery of pre-labeled H1975 and A549 spiked cells transferred on lysine-non-coated and lysine-coated SuperFrost Plus™ adhesion slides. ( Aii ) Immunofluorescence staining among lysine-non-coated and lysine-coated slides; representative staining of leukocyte common antigen (CD45)/DAPI on peripheral blood mononuclear cells (PBMCs) and of cytokeratins (CK)/DAPI on non-pre-labeled A549 cells (Ariol microscopy system, Genetix, New Milton, UK) (400×). ( Bi ) Recovery of pre-labeled cells using different cell harvest approaches. * , ** One-way analysis of variance (ANOVA) test; statistical significance at the p

    Techniques Used: Expressing, Labeling, Immunofluorescence, Staining, Microscopy

    Comparison of the automated CTC-enrichment systems Parsortix and ISET in spiking experiments: Three representative non-small cell lung cancer (NSCLC) cell lines, A549, H1975, and SKMES-1, were used. ( A ) Recovery rates following enrichment by using Parsortix and ISET. Values represent mean recoveries; ± SEM (error bars). * Two-sided t-test; statistical significance observed at the p
    Figure Legend Snippet: Comparison of the automated CTC-enrichment systems Parsortix and ISET in spiking experiments: Three representative non-small cell lung cancer (NSCLC) cell lines, A549, H1975, and SKMES-1, were used. ( A ) Recovery rates following enrichment by using Parsortix and ISET. Values represent mean recoveries; ± SEM (error bars). * Two-sided t-test; statistical significance observed at the p

    Techniques Used:

    5) Product Images from "Optimization of the Enrichment of Circulating Tumor Cells for Downstream Phenotypic Analysis in Patients with Non-Small Cell Lung Cancer Treated with Anti-PD-1 Immunotherapy"

    Article Title: Optimization of the Enrichment of Circulating Tumor Cells for Downstream Phenotypic Analysis in Patients with Non-Small Cell Lung Cancer Treated with Anti-PD-1 Immunotherapy

    Journal: Cancers

    doi: 10.3390/cancers12061556

    Comparison of manual circulating tumor cell (CTC)-enrichment approaches in spiking experiments: ( A ) Recovery rates of pre-labeled A549, H1975, and SKMES-1 cells following enrichment with each method. Values represent mean recoveries; error bars represent standard error of mean (SEM). * , ** One-way ANOVA test; statistical significance at the p
    Figure Legend Snippet: Comparison of manual circulating tumor cell (CTC)-enrichment approaches in spiking experiments: ( A ) Recovery rates of pre-labeled A549, H1975, and SKMES-1 cells following enrichment with each method. Values represent mean recoveries; error bars represent standard error of mean (SEM). * , ** One-way ANOVA test; statistical significance at the p

    Techniques Used: Labeling

    Optimization of cell harvest on slides for downstream protein expression analysis: ( Ai ) Recovery of pre-labeled H1975 and A549 spiked cells transferred on lysine-non-coated and lysine-coated SuperFrost Plus™ adhesion slides. ( Aii ) Immunofluorescence staining among lysine-non-coated and lysine-coated slides; representative staining of leukocyte common antigen (CD45)/DAPI on peripheral blood mononuclear cells (PBMCs) and of cytokeratins (CK)/DAPI on non-pre-labeled A549 cells (Ariol microscopy system, Genetix, New Milton, UK) (400×). ( Bi ) Recovery of pre-labeled cells using different cell harvest approaches. * , ** One-way analysis of variance (ANOVA) test; statistical significance at the p
    Figure Legend Snippet: Optimization of cell harvest on slides for downstream protein expression analysis: ( Ai ) Recovery of pre-labeled H1975 and A549 spiked cells transferred on lysine-non-coated and lysine-coated SuperFrost Plus™ adhesion slides. ( Aii ) Immunofluorescence staining among lysine-non-coated and lysine-coated slides; representative staining of leukocyte common antigen (CD45)/DAPI on peripheral blood mononuclear cells (PBMCs) and of cytokeratins (CK)/DAPI on non-pre-labeled A549 cells (Ariol microscopy system, Genetix, New Milton, UK) (400×). ( Bi ) Recovery of pre-labeled cells using different cell harvest approaches. * , ** One-way analysis of variance (ANOVA) test; statistical significance at the p

    Techniques Used: Expressing, Labeling, Immunofluorescence, Staining, Microscopy

    Comparison of the automated CTC-enrichment systems Parsortix and ISET in spiking experiments: Three representative non-small cell lung cancer (NSCLC) cell lines, A549, H1975, and SKMES-1, were used. ( A ) Recovery rates following enrichment by using Parsortix and ISET. Values represent mean recoveries; ± SEM (error bars). * Two-sided t-test; statistical significance observed at the p
    Figure Legend Snippet: Comparison of the automated CTC-enrichment systems Parsortix and ISET in spiking experiments: Three representative non-small cell lung cancer (NSCLC) cell lines, A549, H1975, and SKMES-1, were used. ( A ) Recovery rates following enrichment by using Parsortix and ISET. Values represent mean recoveries; ± SEM (error bars). * Two-sided t-test; statistical significance observed at the p

    Techniques Used:

    6) Product Images from "Distinct Roles of the Adenovirus E4 ORF3 Protein in Viral DNA Replication and Inhibition of Genome Concatenation"

    Article Title: Distinct Roles of the Adenovirus E4 ORF3 Protein in Viral DNA Replication and Inhibition of Genome Concatenation

    Journal: Journal of Virology

    doi: 10.1128/JVI.77.9.5295-5304.2003

    Localization of PML and viral proteins. A549 cells were infected with wild-type or mutant viruses at a multiplicity of 200 particles per cell. (A) Cells were infected for 4 h, and the POD structure was visualized with anti-PML antibody and fluorescein isothiocyanate-labeled secondary antibody. Uninf., uninfected. (B) Cells were infected for 14 h, and the POD structure, E4 ORF3 protein, and DBP-containing virus replication centers were visualized with anti-PML, anti-E4 ORF3, and anti-DBP, as described in Materials and Methods.
    Figure Legend Snippet: Localization of PML and viral proteins. A549 cells were infected with wild-type or mutant viruses at a multiplicity of 200 particles per cell. (A) Cells were infected for 4 h, and the POD structure was visualized with anti-PML antibody and fluorescein isothiocyanate-labeled secondary antibody. Uninf., uninfected. (B) Cells were infected for 14 h, and the POD structure, E4 ORF3 protein, and DBP-containing virus replication centers were visualized with anti-PML, anti-E4 ORF3, and anti-DBP, as described in Materials and Methods.

    Techniques Used: Infection, Mutagenesis, Labeling

    Viral protein synthesis in A549 cells. A549 cells were infected with wild-type or mutant viruses at a multiplicity of 200 particles per cell. Cells were infected for 9 h (top) or 18 h (bottom) and lysed in RIPA lysis buffer. Proteins were separated by SDS-12.5% PAGE, transferred to Hybond-P, and probed with the antibodies indicated on the left. The proteins were visualized with ECL reagents.
    Figure Legend Snippet: Viral protein synthesis in A549 cells. A549 cells were infected with wild-type or mutant viruses at a multiplicity of 200 particles per cell. Cells were infected for 9 h (top) or 18 h (bottom) and lysed in RIPA lysis buffer. Proteins were separated by SDS-12.5% PAGE, transferred to Hybond-P, and probed with the antibodies indicated on the left. The proteins were visualized with ECL reagents.

    Techniques Used: Infection, Mutagenesis, Lysis, Polyacrylamide Gel Electrophoresis

    Relocalization of Rad50 by E4 ORF3. A549 cells on coverslips were infected with wild-type or mutant viruses at multiplicity of 200 particles per cell. Cells were infected for 18 h, and the Rad50 and E4 ORF3 proteins were visualized by using anti-Rad50 and anti-E4 ORF3, as described in Materials and Methods.
    Figure Legend Snippet: Relocalization of Rad50 by E4 ORF3. A549 cells on coverslips were infected with wild-type or mutant viruses at multiplicity of 200 particles per cell. Cells were infected for 18 h, and the Rad50 and E4 ORF3 proteins were visualized by using anti-Rad50 and anti-E4 ORF3, as described in Materials and Methods.

    Techniques Used: Infection, Mutagenesis

    Viral DNA accumulation in A549 cells. A549 cells were infected with the wild type or mutant viruses at a multiplicity of 200 particles per cell. Total nuclear DNA was isolated at the times indicated above the blot, diluted as indicated below the blot, and applied to nylon membrane by using a slot blot apparatus. The blots were hybridized with a fluorescently labeled oligonucleotide probe. The signal was detected and quantified with a Molecular Dynamics Storm 860 phosphorimager and ImageQuant software.
    Figure Legend Snippet: Viral DNA accumulation in A549 cells. A549 cells were infected with the wild type or mutant viruses at a multiplicity of 200 particles per cell. Total nuclear DNA was isolated at the times indicated above the blot, diluted as indicated below the blot, and applied to nylon membrane by using a slot blot apparatus. The blots were hybridized with a fluorescently labeled oligonucleotide probe. The signal was detected and quantified with a Molecular Dynamics Storm 860 phosphorimager and ImageQuant software.

    Techniques Used: Infection, Mutagenesis, Isolation, Dot Blot, Labeling, Software

    Ad genome concatemer formation. A549 cells were infected with wild-type or mutant viruses at a multiplicity of 200 particles per cell. Cells were harvested 36 h after infection, solidified in agarose plugs, and treated with SDS-proteinase K solution. The DNA were separated on 1.0% agarose by pulsed-field gel electrophoresis and transferred to Hybond-N. Viral unit-length genome and multimers were hybridized with 32 P-labeled Ad genomic probe. Uninf., uninfected; D, dimer; M, monomer.
    Figure Legend Snippet: Ad genome concatemer formation. A549 cells were infected with wild-type or mutant viruses at a multiplicity of 200 particles per cell. Cells were harvested 36 h after infection, solidified in agarose plugs, and treated with SDS-proteinase K solution. The DNA were separated on 1.0% agarose by pulsed-field gel electrophoresis and transferred to Hybond-N. Viral unit-length genome and multimers were hybridized with 32 P-labeled Ad genomic probe. Uninf., uninfected; D, dimer; M, monomer.

    Techniques Used: Infection, Mutagenesis, Pulsed-Field Gel, Electrophoresis, Labeling

    Related Articles

    other:

    Article Title: Noninvasive Imaging and Quantification of Radiotherapy-Induced PD-L1 Upregulation with 89Zr–Df–Atezolizumab
    Article Snippet: For the first study, protein was simply extracted from H460 and A549 cells at 60% confluence to explore baseline PD-L1 levels.

    Article Title: Anticancer activity of CX-3543: a direct inhibitor of rRNA biogenesis.
    Article Snippet: A549 cells were seeded at 1.5 106 cells per 150-mm plate 1 d before 2-h treatment with 10 Amol/L with CX3543.

    Recombinant:

    Article Title: Characterizing the cellular attachment receptor for Langat virus
    Article Snippet: .. Before binding to reactivated A549 and SW620 cells, LGTV was pre-incubated either without (untreated control) or with 50 and 5 μg/mL of recombinant human LGR6, 25 to 567aa, (Novus Biologicals) in cold PBS, for 1 h on ice at 4°C with agitation. ..

    Binding Assay:

    Article Title: Characterizing the cellular attachment receptor for Langat virus
    Article Snippet: .. Before binding to reactivated A549 and SW620 cells, LGTV was pre-incubated either without (untreated control) or with 50 and 5 μg/mL of recombinant human LGR6, 25 to 567aa, (Novus Biologicals) in cold PBS, for 1 h on ice at 4°C with agitation. ..

    Infection:

    Article Title: Distinct Roles of the Adenovirus E4 ORF3 Protein in Viral DNA Replication and Inhibition of Genome Concatenation
    Article Snippet: .. For Rad50, A549 cells were infected for 18 h and a 1:250 dilution of anti-Rad50 (2C6) mouse monoclonal antibody (Novus Biologicals) was used. ..

    Staining:

    Article Title: Human airway epithelia express catalytically active NEU3 sialidase
    Article Snippet: .. Nonpermeabilized unstimulated 1HAEo− , CFTE29o− , 16HBE14o− , and BEAS-2B cells, SAECs, and A549 cells were stained with rabbit polyclonal anti-human NEU3 antibody (Novus Biologicals, Littleton, CO) or control nonimmune rabbit IgG followed by phycoerythrin-conjugated goat anti-rabbit secondary antibody (Jackson ImmunoResearch). .. Cellular fluorescence was measured with an Accuri C6 Flow Cytometer (Becton Dickinson, Franklin Lakes, NJ) and data were analyzed with FlowJo Software (Tree Star, Ashland, OR) ( , ).

    Article Title: Optimization of the Enrichment of Circulating Tumor Cells for Downstream Phenotypic Analysis in Patients with Non-Small Cell Lung Cancer Treated with Anti-PD-1 Immunotherapy
    Article Snippet: .. For this purpose, A549 cells were plated at a density of 4 × 105 cells in 6 well plates, allowed to adhere, and then treated with different concentrations of IFN-γ (10, 25, 50, 100, and 500 ng/mL) for 24 h and 48 h. Cells were detached with 0.25% Trypsin/EDTA, and cytospins of 5 × 105 cells were prepared in order to serve as controls for the optimization of the CK/IDO/PD-L1 staining. .. The optimum induction of IDO expression in A549 cells was obtained after IFN-γ treatment at 100 ng/mL for 48 h ( ).

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    Novus Biologicals a549 cells
    NEU3 surface expression on airway ECs. Flow cytometry of surface NEU3 on ( A ) 1HAEo − , ( B ) CFTE29o − , ( C ) 16HBE14o − , ( D ) BEAS-2B, ( E ) SAEC, and ( F ) <t>A549</t> airway ECs. The gray-shaded histograms represent staining with control nonimmune
    A549 Cells, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a549 cells/product/Novus Biologicals
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a549 cells - by Bioz Stars, 2021-01
    94/100 stars
      Buy from Supplier


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    NEU3 surface expression on airway ECs. Flow cytometry of surface NEU3 on ( A ) 1HAEo − , ( B ) CFTE29o − , ( C ) 16HBE14o − , ( D ) BEAS-2B, ( E ) SAEC, and ( F ) A549 airway ECs. The gray-shaded histograms represent staining with control nonimmune

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Human airway epithelia express catalytically active NEU3 sialidase

    doi: 10.1152/ajplung.00322.2013

    Figure Lengend Snippet: NEU3 surface expression on airway ECs. Flow cytometry of surface NEU3 on ( A ) 1HAEo − , ( B ) CFTE29o − , ( C ) 16HBE14o − , ( D ) BEAS-2B, ( E ) SAEC, and ( F ) A549 airway ECs. The gray-shaded histograms represent staining with control nonimmune

    Article Snippet: Nonpermeabilized unstimulated 1HAEo− , CFTE29o− , 16HBE14o− , and BEAS-2B cells, SAECs, and A549 cells were stained with rabbit polyclonal anti-human NEU3 antibody (Novus Biologicals, Littleton, CO) or control nonimmune rabbit IgG followed by phycoerythrin-conjugated goat anti-rabbit secondary antibody (Jackson ImmunoResearch).

    Techniques: Expressing, Flow Cytometry, Cytometry, Staining

    Airway epithelial cell (EC) sialidase activity. A : increasing primary small airway EC (SAEC; n = 2) and A549 ( n = 3) cell numbers were assayed for sialidase activity for a ganglioside mixture. B : A549 cells (1.0 × 10 6 ) were assayed for sialidase

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Human airway epithelia express catalytically active NEU3 sialidase

    doi: 10.1152/ajplung.00322.2013

    Figure Lengend Snippet: Airway epithelial cell (EC) sialidase activity. A : increasing primary small airway EC (SAEC; n = 2) and A549 ( n = 3) cell numbers were assayed for sialidase activity for a ganglioside mixture. B : A549 cells (1.0 × 10 6 ) were assayed for sialidase

    Article Snippet: Nonpermeabilized unstimulated 1HAEo− , CFTE29o− , 16HBE14o− , and BEAS-2B cells, SAECs, and A549 cells were stained with rabbit polyclonal anti-human NEU3 antibody (Novus Biologicals, Littleton, CO) or control nonimmune rabbit IgG followed by phycoerythrin-conjugated goat anti-rabbit secondary antibody (Jackson ImmunoResearch).

    Techniques: Activity Assay

    NEU3 catalytic activity in airway ECs. A–C : A549 cells were infected with increasing multiplicities of infection (MOIs) of Ad-NEU3-HA and cultured for 48 h. A : cells were lysed and the lysates were processed for immunoblotting with anti-hemagglutinin

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Human airway epithelia express catalytically active NEU3 sialidase

    doi: 10.1152/ajplung.00322.2013

    Figure Lengend Snippet: NEU3 catalytic activity in airway ECs. A–C : A549 cells were infected with increasing multiplicities of infection (MOIs) of Ad-NEU3-HA and cultured for 48 h. A : cells were lysed and the lysates were processed for immunoblotting with anti-hemagglutinin

    Article Snippet: Nonpermeabilized unstimulated 1HAEo− , CFTE29o− , 16HBE14o− , and BEAS-2B cells, SAECs, and A549 cells were stained with rabbit polyclonal anti-human NEU3 antibody (Novus Biologicals, Littleton, CO) or control nonimmune rabbit IgG followed by phycoerythrin-conjugated goat anti-rabbit secondary antibody (Jackson ImmunoResearch).

    Techniques: Activity Assay, Infection, Cell Culture

    Subcellular localization of NEU3. A : subconfluent A549 cells were probed with anti-NEU3 antibody ( I and ii ), or nonimmune IgG as a negative control ( iii ), and counterstained with DAPI (overlays in i and iii ). Arrows indicate the nuclear regions in NEU3-stained

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Human airway epithelia express catalytically active NEU3 sialidase

    doi: 10.1152/ajplung.00322.2013

    Figure Lengend Snippet: Subcellular localization of NEU3. A : subconfluent A549 cells were probed with anti-NEU3 antibody ( I and ii ), or nonimmune IgG as a negative control ( iii ), and counterstained with DAPI (overlays in i and iii ). Arrows indicate the nuclear regions in NEU3-stained

    Article Snippet: Nonpermeabilized unstimulated 1HAEo− , CFTE29o− , 16HBE14o− , and BEAS-2B cells, SAECs, and A549 cells were stained with rabbit polyclonal anti-human NEU3 antibody (Novus Biologicals, Littleton, CO) or control nonimmune rabbit IgG followed by phycoerythrin-conjugated goat anti-rabbit secondary antibody (Jackson ImmunoResearch).

    Techniques: Negative Control, Staining

    Verification of PD-L1 protein expression on EVs. ( A ) Fluorescence signals of PBS only (no EVs and no antibodies), EV only (EVs without staining with any antibodies), EV isotype (EVs stained with isotype control antibody), and EV CD63 antibodies (Abs) (EVs stained with CD63 antibody). ( B ) Fluorescence signals of PBS only, EV only, EV isotype, and EV PD-L1 Abs (EVs stained with PD-L1 antibody). ( C ) Particle size and zeta potential measurement of the EVs isolated from A549 cells overexpressed with CD63-GFP by nanoparticle tracking analysis technique. d.nm, diameter (nm). ( D ) EVs isolated from human A549 cells overexpressed with CD63-GFP or PD-L1–mCherry ( E ) and diluted to different concentrations. 1:1, initial concentration; 1:10, 10-fold dilution; 1:100, 100-fold dilution; 1:1000, 1000-fold dilution.

    Journal: Science Advances

    Article Title: High-throughput single-EV liquid biopsy: Rapid, simultaneous, and multiplexed detection of nucleic acids, proteins, and their combinations

    doi: 10.1126/sciadv.abc1204

    Figure Lengend Snippet: Verification of PD-L1 protein expression on EVs. ( A ) Fluorescence signals of PBS only (no EVs and no antibodies), EV only (EVs without staining with any antibodies), EV isotype (EVs stained with isotype control antibody), and EV CD63 antibodies (Abs) (EVs stained with CD63 antibody). ( B ) Fluorescence signals of PBS only, EV only, EV isotype, and EV PD-L1 Abs (EVs stained with PD-L1 antibody). ( C ) Particle size and zeta potential measurement of the EVs isolated from A549 cells overexpressed with CD63-GFP by nanoparticle tracking analysis technique. d.nm, diameter (nm). ( D ) EVs isolated from human A549 cells overexpressed with CD63-GFP or PD-L1–mCherry ( E ) and diluted to different concentrations. 1:1, initial concentration; 1:10, 10-fold dilution; 1:100, 100-fold dilution; 1:1000, 1000-fold dilution.

    Article Snippet: Immunofluorescence staining for the membrane protein of cellsTo stain CD63 or PD-L1 membrane protein, A549 cells were fixed with 4% paraformaldehyde and stained with CD63-AF488 (NBP2-42225AF488; Novus Biologicals, Littleton, CO, USA) or PD-L1–AF647 (ab209960; Abcam, Cambridge, MA, USA) fluorescent antibodies, respectively (table S3).

    Techniques: Expressing, Fluorescence, Staining, Isolation, Concentration Assay

    Double immunofluorescence staining of CD63 and PD-L1 membrane protein. ( A ) EVs isolated from 1000 μl of cell supernatant of human A549 cells overexpressed with CD63-GFP and PD-L1–mCherry. The dots in the colocalized only image represent EVs expressing both CD63 and PD-L1 protein. ( B ) EVs isolated from 90 μl of human plasma are stained with CD63-AF488 and PD-L1–AF647 antibodies. The dots in the colocalized only image represent EVs expressing both CD63 and PD-L1 protein. ( C ) Ratio of CD63- and PD-L1–positive EVs to CD63-positive EVs isolated from human A549 cells overexpressed with CD63-GFP and PD-L1–mCherry or isolated from plasma of 20 patients with LUAD.

    Journal: Science Advances

    Article Title: High-throughput single-EV liquid biopsy: Rapid, simultaneous, and multiplexed detection of nucleic acids, proteins, and their combinations

    doi: 10.1126/sciadv.abc1204

    Figure Lengend Snippet: Double immunofluorescence staining of CD63 and PD-L1 membrane protein. ( A ) EVs isolated from 1000 μl of cell supernatant of human A549 cells overexpressed with CD63-GFP and PD-L1–mCherry. The dots in the colocalized only image represent EVs expressing both CD63 and PD-L1 protein. ( B ) EVs isolated from 90 μl of human plasma are stained with CD63-AF488 and PD-L1–AF647 antibodies. The dots in the colocalized only image represent EVs expressing both CD63 and PD-L1 protein. ( C ) Ratio of CD63- and PD-L1–positive EVs to CD63-positive EVs isolated from human A549 cells overexpressed with CD63-GFP and PD-L1–mCherry or isolated from plasma of 20 patients with LUAD.

    Article Snippet: Immunofluorescence staining for the membrane protein of cellsTo stain CD63 or PD-L1 membrane protein, A549 cells were fixed with 4% paraformaldehyde and stained with CD63-AF488 (NBP2-42225AF488; Novus Biologicals, Littleton, CO, USA) or PD-L1–AF647 (ab209960; Abcam, Cambridge, MA, USA) fluorescent antibodies, respectively (table S3).

    Techniques: Double Immunofluorescence Staining, Isolation, Expressing, Staining

    Measurement of EV mRNA and protein expression in vitro. ( A ) RT-qPCR and ( B ) Western blotting analysis of PD-L1 expression of vector and PD-L1–overexpressed A549 cells. ( C ) HNCIB analysis of PD-L1 mRNA and protein expression of EVs isolated from supernatant of A549 cells overexpressing vector and PD-L1 ( D ). ** P

    Journal: Science Advances

    Article Title: High-throughput single-EV liquid biopsy: Rapid, simultaneous, and multiplexed detection of nucleic acids, proteins, and their combinations

    doi: 10.1126/sciadv.abc1204

    Figure Lengend Snippet: Measurement of EV mRNA and protein expression in vitro. ( A ) RT-qPCR and ( B ) Western blotting analysis of PD-L1 expression of vector and PD-L1–overexpressed A549 cells. ( C ) HNCIB analysis of PD-L1 mRNA and protein expression of EVs isolated from supernatant of A549 cells overexpressing vector and PD-L1 ( D ). ** P

    Article Snippet: Immunofluorescence staining for the membrane protein of cellsTo stain CD63 or PD-L1 membrane protein, A549 cells were fixed with 4% paraformaldehyde and stained with CD63-AF488 (NBP2-42225AF488; Novus Biologicals, Littleton, CO, USA) or PD-L1–AF647 (ab209960; Abcam, Cambridge, MA, USA) fluorescent antibodies, respectively (table S3).

    Techniques: Expressing, In Vitro, Quantitative RT-PCR, Western Blot, Plasmid Preparation, Isolation

    A549 PD-L1 PET. PET imaging of mice with PD-L1(−) A549 xenografts with 89 Zr–Df–atezolizumab. Tumors are indicated by red dashed circles in the representative MIPs.

    Journal: Bioconjugate chemistry

    Article Title: Noninvasive Imaging and Quantification of Radiotherapy-Induced PD-L1 Upregulation with 89Zr–Df–Atezolizumab

    doi: 10.1021/acs.bioconjchem.9b00178

    Figure Lengend Snippet: A549 PD-L1 PET. PET imaging of mice with PD-L1(−) A549 xenografts with 89 Zr–Df–atezolizumab. Tumors are indicated by red dashed circles in the representative MIPs.

    Article Snippet: For the first study, protein was simply extracted from H460 and A549 cells at 60% confluence to explore baseline PD-L1 levels.

    Techniques: Positron Emission Tomography, Imaging, Mouse Assay

    Quantification of tracer biodistribution. (A) Uptake of 89 Zr–Df–atezolizumab in H460 tumors as determined through region-of-interest analysis of PET images. (B) Tumor-to-muscle ratios for all studied H460 groups over time. Both analyses indicate the highest accumulation of 89 Zr–Df–atezolizumab in the fractionated radiotherapy group. (C) Tumor uptake over time in H460 and A549 tumor xenografts receiving 2 Gy × 5 Fx. (D) Ex vivo biodistribution study results in the two tumor models at 96 h postinjection of tracers. * p

    Journal: Bioconjugate chemistry

    Article Title: Noninvasive Imaging and Quantification of Radiotherapy-Induced PD-L1 Upregulation with 89Zr–Df–Atezolizumab

    doi: 10.1021/acs.bioconjchem.9b00178

    Figure Lengend Snippet: Quantification of tracer biodistribution. (A) Uptake of 89 Zr–Df–atezolizumab in H460 tumors as determined through region-of-interest analysis of PET images. (B) Tumor-to-muscle ratios for all studied H460 groups over time. Both analyses indicate the highest accumulation of 89 Zr–Df–atezolizumab in the fractionated radiotherapy group. (C) Tumor uptake over time in H460 and A549 tumor xenografts receiving 2 Gy × 5 Fx. (D) Ex vivo biodistribution study results in the two tumor models at 96 h postinjection of tracers. * p

    Article Snippet: For the first study, protein was simply extracted from H460 and A549 cells at 60% confluence to explore baseline PD-L1 levels.

    Techniques: Positron Emission Tomography, Ex Vivo

    A549 tumor staining. Minimal PD-L1 staining was observed in all groups of A549 tumor-bearing mice. Scale bar = 20 μ m.

    Journal: Bioconjugate chemistry

    Article Title: Noninvasive Imaging and Quantification of Radiotherapy-Induced PD-L1 Upregulation with 89Zr–Df–Atezolizumab

    doi: 10.1021/acs.bioconjchem.9b00178

    Figure Lengend Snippet: A549 tumor staining. Minimal PD-L1 staining was observed in all groups of A549 tumor-bearing mice. Scale bar = 20 μ m.

    Article Snippet: For the first study, protein was simply extracted from H460 and A549 cells at 60% confluence to explore baseline PD-L1 levels.

    Techniques: Staining, Mouse Assay

    Comparison of manual circulating tumor cell (CTC)-enrichment approaches in spiking experiments: ( A ) Recovery rates of pre-labeled A549, H1975, and SKMES-1 cells following enrichment with each method. Values represent mean recoveries; error bars represent standard error of mean (SEM). * , ** One-way ANOVA test; statistical significance at the p

    Journal: Cancers

    Article Title: Optimization of the Enrichment of Circulating Tumor Cells for Downstream Phenotypic Analysis in Patients with Non-Small Cell Lung Cancer Treated with Anti-PD-1 Immunotherapy

    doi: 10.3390/cancers12061556

    Figure Lengend Snippet: Comparison of manual circulating tumor cell (CTC)-enrichment approaches in spiking experiments: ( A ) Recovery rates of pre-labeled A549, H1975, and SKMES-1 cells following enrichment with each method. Values represent mean recoveries; error bars represent standard error of mean (SEM). * , ** One-way ANOVA test; statistical significance at the p

    Article Snippet: For this purpose, A549 cells were plated at a density of 4 × 105 cells in 6 well plates, allowed to adhere, and then treated with different concentrations of IFN-γ (10, 25, 50, 100, and 500 ng/mL) for 24 h and 48 h. Cells were detached with 0.25% Trypsin/EDTA, and cytospins of 5 × 105 cells were prepared in order to serve as controls for the optimization of the CK/IDO/PD-L1 staining.

    Techniques: Labeling

    Optimization of cell harvest on slides for downstream protein expression analysis: ( Ai ) Recovery of pre-labeled H1975 and A549 spiked cells transferred on lysine-non-coated and lysine-coated SuperFrost Plus™ adhesion slides. ( Aii ) Immunofluorescence staining among lysine-non-coated and lysine-coated slides; representative staining of leukocyte common antigen (CD45)/DAPI on peripheral blood mononuclear cells (PBMCs) and of cytokeratins (CK)/DAPI on non-pre-labeled A549 cells (Ariol microscopy system, Genetix, New Milton, UK) (400×). ( Bi ) Recovery of pre-labeled cells using different cell harvest approaches. * , ** One-way analysis of variance (ANOVA) test; statistical significance at the p

    Journal: Cancers

    Article Title: Optimization of the Enrichment of Circulating Tumor Cells for Downstream Phenotypic Analysis in Patients with Non-Small Cell Lung Cancer Treated with Anti-PD-1 Immunotherapy

    doi: 10.3390/cancers12061556

    Figure Lengend Snippet: Optimization of cell harvest on slides for downstream protein expression analysis: ( Ai ) Recovery of pre-labeled H1975 and A549 spiked cells transferred on lysine-non-coated and lysine-coated SuperFrost Plus™ adhesion slides. ( Aii ) Immunofluorescence staining among lysine-non-coated and lysine-coated slides; representative staining of leukocyte common antigen (CD45)/DAPI on peripheral blood mononuclear cells (PBMCs) and of cytokeratins (CK)/DAPI on non-pre-labeled A549 cells (Ariol microscopy system, Genetix, New Milton, UK) (400×). ( Bi ) Recovery of pre-labeled cells using different cell harvest approaches. * , ** One-way analysis of variance (ANOVA) test; statistical significance at the p

    Article Snippet: For this purpose, A549 cells were plated at a density of 4 × 105 cells in 6 well plates, allowed to adhere, and then treated with different concentrations of IFN-γ (10, 25, 50, 100, and 500 ng/mL) for 24 h and 48 h. Cells were detached with 0.25% Trypsin/EDTA, and cytospins of 5 × 105 cells were prepared in order to serve as controls for the optimization of the CK/IDO/PD-L1 staining.

    Techniques: Expressing, Labeling, Immunofluorescence, Staining, Microscopy

    Comparison of the automated CTC-enrichment systems Parsortix and ISET in spiking experiments: Three representative non-small cell lung cancer (NSCLC) cell lines, A549, H1975, and SKMES-1, were used. ( A ) Recovery rates following enrichment by using Parsortix and ISET. Values represent mean recoveries; ± SEM (error bars). * Two-sided t-test; statistical significance observed at the p

    Journal: Cancers

    Article Title: Optimization of the Enrichment of Circulating Tumor Cells for Downstream Phenotypic Analysis in Patients with Non-Small Cell Lung Cancer Treated with Anti-PD-1 Immunotherapy

    doi: 10.3390/cancers12061556

    Figure Lengend Snippet: Comparison of the automated CTC-enrichment systems Parsortix and ISET in spiking experiments: Three representative non-small cell lung cancer (NSCLC) cell lines, A549, H1975, and SKMES-1, were used. ( A ) Recovery rates following enrichment by using Parsortix and ISET. Values represent mean recoveries; ± SEM (error bars). * Two-sided t-test; statistical significance observed at the p

    Article Snippet: For this purpose, A549 cells were plated at a density of 4 × 105 cells in 6 well plates, allowed to adhere, and then treated with different concentrations of IFN-γ (10, 25, 50, 100, and 500 ng/mL) for 24 h and 48 h. Cells were detached with 0.25% Trypsin/EDTA, and cytospins of 5 × 105 cells were prepared in order to serve as controls for the optimization of the CK/IDO/PD-L1 staining.

    Techniques: