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Nanjing KeyGen Biotech Co Ltd a549 cells
( A ) Antitumor effect of CK and CK mixed micelles to <t>A549</t> cell on different concentrations. * P
A549 Cells, supplied by Nanjing KeyGen Biotech Co Ltd, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo"

Article Title: Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S119226

( A ) Antitumor effect of CK and CK mixed micelles to A549 cell on different concentrations. * P
Figure Legend Snippet: ( A ) Antitumor effect of CK and CK mixed micelles to A549 cell on different concentrations. * P

Techniques Used:

Fluorescence microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.
Figure Legend Snippet: Fluorescence microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.

Techniques Used: Fluorescence, Microscopy

Confocal microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.
Figure Legend Snippet: Confocal microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.

Techniques Used: Confocal Microscopy

Cell cycle analysis assay (green G1, brown S, and blue G2 phase). ( A ) and wound healing assay ( B ) on A549 cell. Notes: n=3, mean ± SE. Scale 50 μm. Abbreviations: CK, compound K; SE, standard error.
Figure Legend Snippet: Cell cycle analysis assay (green G1, brown S, and blue G2 phase). ( A ) and wound healing assay ( B ) on A549 cell. Notes: n=3, mean ± SE. Scale 50 μm. Abbreviations: CK, compound K; SE, standard error.

Techniques Used: Cell Cycle Assay, Wound Healing Assay

2) Product Images from "Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo"

Article Title: Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S119226

( A ) Antitumor effect of CK and CK mixed micelles to A549 cell on different concentrations. * P
Figure Legend Snippet: ( A ) Antitumor effect of CK and CK mixed micelles to A549 cell on different concentrations. * P

Techniques Used:

Fluorescence microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.
Figure Legend Snippet: Fluorescence microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.

Techniques Used: Fluorescence, Microscopy

Confocal microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.
Figure Legend Snippet: Confocal microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.

Techniques Used: Confocal Microscopy

Cell cycle analysis assay (green G1, brown S, and blue G2 phase). ( A ) and wound healing assay ( B ) on A549 cell. Notes: n=3, mean ± SE. Scale 50 μm. Abbreviations: CK, compound K; SE, standard error.
Figure Legend Snippet: Cell cycle analysis assay (green G1, brown S, and blue G2 phase). ( A ) and wound healing assay ( B ) on A549 cell. Notes: n=3, mean ± SE. Scale 50 μm. Abbreviations: CK, compound K; SE, standard error.

Techniques Used: Cell Cycle Assay, Wound Healing Assay

3) Product Images from "Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo"

Article Title: Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S119226

( A ) Antitumor effect of CK and CK mixed micelles to A549 cell on different concentrations. * P
Figure Legend Snippet: ( A ) Antitumor effect of CK and CK mixed micelles to A549 cell on different concentrations. * P

Techniques Used:

Fluorescence microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.
Figure Legend Snippet: Fluorescence microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.

Techniques Used: Fluorescence, Microscopy

Confocal microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.
Figure Legend Snippet: Confocal microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.

Techniques Used: Confocal Microscopy

Cell cycle analysis assay (green G1, brown S, and blue G2 phase). ( A ) and wound healing assay ( B ) on A549 cell. Notes: n=3, mean ± SE. Scale 50 μm. Abbreviations: CK, compound K; SE, standard error.
Figure Legend Snippet: Cell cycle analysis assay (green G1, brown S, and blue G2 phase). ( A ) and wound healing assay ( B ) on A549 cell. Notes: n=3, mean ± SE. Scale 50 μm. Abbreviations: CK, compound K; SE, standard error.

Techniques Used: Cell Cycle Assay, Wound Healing Assay

4) Product Images from "Metformin inhibits growth of lung adenocarcinoma cells by inducing apoptosis via the mitochondria-mediated pathway"

Article Title: Metformin inhibits growth of lung adenocarcinoma cells by inducing apoptosis via the mitochondria-mediated pathway

Journal: Oncology Letters

doi: 10.3892/ol.2015.3450

Effects of metformin on the proliferation of A549 cells. (A) Metformin inhibited the proliferation of the cells in a dose- and time-dependent manner. The A549 cells were exposed to increasing doses of metformin for 24, 48 and 72 h respectively, and cell
Figure Legend Snippet: Effects of metformin on the proliferation of A549 cells. (A) Metformin inhibited the proliferation of the cells in a dose- and time-dependent manner. The A549 cells were exposed to increasing doses of metformin for 24, 48 and 72 h respectively, and cell

Techniques Used:

Metformin induces apoptosis of A549 cells. (A) A549 cells were treated with 10 mM metformin for 48 h. Images of the cells were captured under a light microscope, or the cells were stained with Hoechst 33342 and visualized under a fluorescence microscope.
Figure Legend Snippet: Metformin induces apoptosis of A549 cells. (A) A549 cells were treated with 10 mM metformin for 48 h. Images of the cells were captured under a light microscope, or the cells were stained with Hoechst 33342 and visualized under a fluorescence microscope.

Techniques Used: Light Microscopy, Staining, Fluorescence, Microscopy

Metformin blocks the cell cycle in the G 0 /G 1 phase. Flow cytometry analysis of proliferating A549 cells 48 h after the treatment with metformin (10 and 20 mM). Fractions of cells in the G 0 /G 1 , S and G 2 /M phases of the cell cycle are indicted. Untreated
Figure Legend Snippet: Metformin blocks the cell cycle in the G 0 /G 1 phase. Flow cytometry analysis of proliferating A549 cells 48 h after the treatment with metformin (10 and 20 mM). Fractions of cells in the G 0 /G 1 , S and G 2 /M phases of the cell cycle are indicted. Untreated

Techniques Used: Flow Cytometry, Cytometry

Metformin decreases A549 xenograft tumor proliferation. (A) The final tumor weight at necropsy at 18 days post-administration. (B) Effect of metformin on the body weight of mice during 18 days of treatment. (C) Examples of tumor regression in metformin-treated
Figure Legend Snippet: Metformin decreases A549 xenograft tumor proliferation. (A) The final tumor weight at necropsy at 18 days post-administration. (B) Effect of metformin on the body weight of mice during 18 days of treatment. (C) Examples of tumor regression in metformin-treated

Techniques Used: Mouse Assay

Metformin induces apoptosis of A549 cells mainly through the mitochondia-mediated pathway. (A) Effect of metformin on the expression of apoptosis-regulating proteins, as assessed by western blotting. Protein fractions of total cells were assessed for
Figure Legend Snippet: Metformin induces apoptosis of A549 cells mainly through the mitochondia-mediated pathway. (A) Effect of metformin on the expression of apoptosis-regulating proteins, as assessed by western blotting. Protein fractions of total cells were assessed for

Techniques Used: Expressing, Western Blot

5) Product Images from "Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo"

Article Title: Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S119226

( A ) Antitumor effect of CK and CK mixed micelles to A549 cell on different concentrations. * P
Figure Legend Snippet: ( A ) Antitumor effect of CK and CK mixed micelles to A549 cell on different concentrations. * P

Techniques Used:

Fluorescence microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.
Figure Legend Snippet: Fluorescence microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.

Techniques Used: Fluorescence, Microscopy

Confocal microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.
Figure Legend Snippet: Confocal microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.

Techniques Used: Confocal Microscopy

Cell cycle analysis assay (green G1, brown S, and blue G2 phase). ( A ) and wound healing assay ( B ) on A549 cell. Notes: n=3, mean ± SE. Scale 50 μm. Abbreviations: CK, compound K; SE, standard error.
Figure Legend Snippet: Cell cycle analysis assay (green G1, brown S, and blue G2 phase). ( A ) and wound healing assay ( B ) on A549 cell. Notes: n=3, mean ± SE. Scale 50 μm. Abbreviations: CK, compound K; SE, standard error.

Techniques Used: Cell Cycle Assay, Wound Healing Assay

6) Product Images from "Combination treatment of gemcitabine and sorafenib exerts a synergistic inhibitory effect on non-small cell lung cancer in vitro and in vivo via the epithelial-to-mesenchymal transition process"

Article Title: Combination treatment of gemcitabine and sorafenib exerts a synergistic inhibitory effect on non-small cell lung cancer in vitro and in vivo via the epithelial-to-mesenchymal transition process

Journal: Oncology Letters

doi: 10.3892/ol.2020.11536

Co-treatment with gemcitabine and sorafenib induces apoptosis and cell cycle arrest in A549 cells. A549 cells were treated with 10 µg/ml gemcitabine and 10 µM sorafenib, either alone or in combination for 48 h. (A) Cell cycle distribution was determined by flow cytometry analysis. (B) The quantification of cells in different phases of the cell cycle is presented in bar charts. (C) The percentage of apoptotic cells was determined by flow cytometry analysis. PI, propidium iodide. (D) The percentage of A549 cells positive for Annexin V staining was presented in bar charts. *P
Figure Legend Snippet: Co-treatment with gemcitabine and sorafenib induces apoptosis and cell cycle arrest in A549 cells. A549 cells were treated with 10 µg/ml gemcitabine and 10 µM sorafenib, either alone or in combination for 48 h. (A) Cell cycle distribution was determined by flow cytometry analysis. (B) The quantification of cells in different phases of the cell cycle is presented in bar charts. (C) The percentage of apoptotic cells was determined by flow cytometry analysis. PI, propidium iodide. (D) The percentage of A549 cells positive for Annexin V staining was presented in bar charts. *P

Techniques Used: Flow Cytometry, Staining

Effects of co-treatment with gemcitabine and sorafenib on migration of A549 cells. (A) A549 cells were treated with a combination of 10 µg/ml gemcitabine and 10 µM sorafenib for 48 h, and cell migration was determined by a wound healing assay using an inverted microscope (magnification, ×200). (B) Quantification of A549 migratory cells. *P
Figure Legend Snippet: Effects of co-treatment with gemcitabine and sorafenib on migration of A549 cells. (A) A549 cells were treated with a combination of 10 µg/ml gemcitabine and 10 µM sorafenib for 48 h, and cell migration was determined by a wound healing assay using an inverted microscope (magnification, ×200). (B) Quantification of A549 migratory cells. *P

Techniques Used: Migration, Wound Healing Assay, Inverted Microscopy

Dose-dependent cytotoxic effect of gemcitabine and sorafenib on A549 cells. (A and B) The molecular structure of (A) gemcitabine and (B) sorafenib. (C and D) A549 cells were treated with (C) 0–100 µg/ml of gemcitabine and (D) 0–100 µM of sorafenib for 48 h, and cell viability was determined by an MTT assay. *P
Figure Legend Snippet: Dose-dependent cytotoxic effect of gemcitabine and sorafenib on A549 cells. (A and B) The molecular structure of (A) gemcitabine and (B) sorafenib. (C and D) A549 cells were treated with (C) 0–100 µg/ml of gemcitabine and (D) 0–100 µM of sorafenib for 48 h, and cell viability was determined by an MTT assay. *P

Techniques Used: MTT Assay

Synergistic antitumor effects of gemcitabine and sorafenib on A549 cells. (A) A549 cells were treated with 5 or 10 µg/ml gemcitabine and 5 or 10 µM sorafenib, either alone or in combination for 48 h, and cell viability was determined by an MTT assay. (B) A549 cells were treated with a combination of 10 µg/ml gemcitabine and 10 µM sorafenib for 48 h, and cell morphology was subsequently observed using an inverted microscope. (C) H1975 and (D) H1650 cells were treated with 10 µg/ml gemcitabine and 10 µM sorafenib, either alone or in combination for 48 h, and cell viability was determined by an MTT assay. *P
Figure Legend Snippet: Synergistic antitumor effects of gemcitabine and sorafenib on A549 cells. (A) A549 cells were treated with 5 or 10 µg/ml gemcitabine and 5 or 10 µM sorafenib, either alone or in combination for 48 h, and cell viability was determined by an MTT assay. (B) A549 cells were treated with a combination of 10 µg/ml gemcitabine and 10 µM sorafenib for 48 h, and cell morphology was subsequently observed using an inverted microscope. (C) H1975 and (D) H1650 cells were treated with 10 µg/ml gemcitabine and 10 µM sorafenib, either alone or in combination for 48 h, and cell viability was determined by an MTT assay. *P

Techniques Used: MTT Assay, Inverted Microscopy

Combination treatment with gemcitabine and sorafenib induces EMT inhibition of A549 cells. A549 cells were treated with a combination of 10 µg/ml gemcitabine and 10 µM sorafenib for 48 h. (A) Protein expression levels of EMT-associated markers N-cadherin, E-cadherin and Twist-1 were assessed by western blot analysis. (B-D) Quantitative analysis of (B) E-cadherin, (C) N-cadherin and (D) Twist-1. (E-G) mRNA expression levels of (E) E-cadherin, (F) N-cadherin and (G) Twist-1. *P
Figure Legend Snippet: Combination treatment with gemcitabine and sorafenib induces EMT inhibition of A549 cells. A549 cells were treated with a combination of 10 µg/ml gemcitabine and 10 µM sorafenib for 48 h. (A) Protein expression levels of EMT-associated markers N-cadherin, E-cadherin and Twist-1 were assessed by western blot analysis. (B-D) Quantitative analysis of (B) E-cadherin, (C) N-cadherin and (D) Twist-1. (E-G) mRNA expression levels of (E) E-cadherin, (F) N-cadherin and (G) Twist-1. *P

Techniques Used: Inhibition, Expressing, Western Blot

Effects of co-treatment with gemcitabine and sorafenib on invasion of A549 cells. (A) The result of cell invasion. (B) Quantification of A549 invasive cells. *P
Figure Legend Snippet: Effects of co-treatment with gemcitabine and sorafenib on invasion of A549 cells. (A) The result of cell invasion. (B) Quantification of A549 invasive cells. *P

Techniques Used:

7) Product Images from "Combination treatment of gemcitabine and sorafenib exerts a synergistic inhibitory effect on non-small cell lung cancer in vitro and in vivo via the epithelial-to-mesenchymal transition process"

Article Title: Combination treatment of gemcitabine and sorafenib exerts a synergistic inhibitory effect on non-small cell lung cancer in vitro and in vivo via the epithelial-to-mesenchymal transition process

Journal: Oncology Letters

doi: 10.3892/ol.2020.11536

Co-treatment with gemcitabine and sorafenib induces apoptosis and cell cycle arrest in A549 cells. A549 cells were treated with 10 µg/ml gemcitabine and 10 µM sorafenib, either alone or in combination for 48 h. (A) Cell cycle distribution was determined by flow cytometry analysis. (B) The quantification of cells in different phases of the cell cycle is presented in bar charts. (C) The percentage of apoptotic cells was determined by flow cytometry analysis. PI, propidium iodide. (D) The percentage of A549 cells positive for Annexin V staining was presented in bar charts. *P
Figure Legend Snippet: Co-treatment with gemcitabine and sorafenib induces apoptosis and cell cycle arrest in A549 cells. A549 cells were treated with 10 µg/ml gemcitabine and 10 µM sorafenib, either alone or in combination for 48 h. (A) Cell cycle distribution was determined by flow cytometry analysis. (B) The quantification of cells in different phases of the cell cycle is presented in bar charts. (C) The percentage of apoptotic cells was determined by flow cytometry analysis. PI, propidium iodide. (D) The percentage of A549 cells positive for Annexin V staining was presented in bar charts. *P

Techniques Used: Flow Cytometry, Staining

Effects of co-treatment with gemcitabine and sorafenib on migration of A549 cells. (A) A549 cells were treated with a combination of 10 µg/ml gemcitabine and 10 µM sorafenib for 48 h, and cell migration was determined by a wound healing assay using an inverted microscope (magnification, ×200). (B) Quantification of A549 migratory cells. *P
Figure Legend Snippet: Effects of co-treatment with gemcitabine and sorafenib on migration of A549 cells. (A) A549 cells were treated with a combination of 10 µg/ml gemcitabine and 10 µM sorafenib for 48 h, and cell migration was determined by a wound healing assay using an inverted microscope (magnification, ×200). (B) Quantification of A549 migratory cells. *P

Techniques Used: Migration, Wound Healing Assay, Inverted Microscopy

Dose-dependent cytotoxic effect of gemcitabine and sorafenib on A549 cells. (A and B) The molecular structure of (A) gemcitabine and (B) sorafenib. (C and D) A549 cells were treated with (C) 0–100 µg/ml of gemcitabine and (D) 0–100 µM of sorafenib for 48 h, and cell viability was determined by an MTT assay. *P
Figure Legend Snippet: Dose-dependent cytotoxic effect of gemcitabine and sorafenib on A549 cells. (A and B) The molecular structure of (A) gemcitabine and (B) sorafenib. (C and D) A549 cells were treated with (C) 0–100 µg/ml of gemcitabine and (D) 0–100 µM of sorafenib for 48 h, and cell viability was determined by an MTT assay. *P

Techniques Used: MTT Assay

Synergistic antitumor effects of gemcitabine and sorafenib on A549 cells. (A) A549 cells were treated with 5 or 10 µg/ml gemcitabine and 5 or 10 µM sorafenib, either alone or in combination for 48 h, and cell viability was determined by an MTT assay. (B) A549 cells were treated with a combination of 10 µg/ml gemcitabine and 10 µM sorafenib for 48 h, and cell morphology was subsequently observed using an inverted microscope. (C) H1975 and (D) H1650 cells were treated with 10 µg/ml gemcitabine and 10 µM sorafenib, either alone or in combination for 48 h, and cell viability was determined by an MTT assay. *P
Figure Legend Snippet: Synergistic antitumor effects of gemcitabine and sorafenib on A549 cells. (A) A549 cells were treated with 5 or 10 µg/ml gemcitabine and 5 or 10 µM sorafenib, either alone or in combination for 48 h, and cell viability was determined by an MTT assay. (B) A549 cells were treated with a combination of 10 µg/ml gemcitabine and 10 µM sorafenib for 48 h, and cell morphology was subsequently observed using an inverted microscope. (C) H1975 and (D) H1650 cells were treated with 10 µg/ml gemcitabine and 10 µM sorafenib, either alone or in combination for 48 h, and cell viability was determined by an MTT assay. *P

Techniques Used: MTT Assay, Inverted Microscopy

Combination treatment with gemcitabine and sorafenib induces EMT inhibition of A549 cells. A549 cells were treated with a combination of 10 µg/ml gemcitabine and 10 µM sorafenib for 48 h. (A) Protein expression levels of EMT-associated markers N-cadherin, E-cadherin and Twist-1 were assessed by western blot analysis. (B-D) Quantitative analysis of (B) E-cadherin, (C) N-cadherin and (D) Twist-1. (E-G) mRNA expression levels of (E) E-cadherin, (F) N-cadherin and (G) Twist-1. *P
Figure Legend Snippet: Combination treatment with gemcitabine and sorafenib induces EMT inhibition of A549 cells. A549 cells were treated with a combination of 10 µg/ml gemcitabine and 10 µM sorafenib for 48 h. (A) Protein expression levels of EMT-associated markers N-cadherin, E-cadherin and Twist-1 were assessed by western blot analysis. (B-D) Quantitative analysis of (B) E-cadherin, (C) N-cadherin and (D) Twist-1. (E-G) mRNA expression levels of (E) E-cadherin, (F) N-cadherin and (G) Twist-1. *P

Techniques Used: Inhibition, Expressing, Western Blot

Effects of co-treatment with gemcitabine and sorafenib on invasion of A549 cells. (A) The result of cell invasion. (B) Quantification of A549 invasive cells. *P
Figure Legend Snippet: Effects of co-treatment with gemcitabine and sorafenib on invasion of A549 cells. (A) The result of cell invasion. (B) Quantification of A549 invasive cells. *P

Techniques Used:

8) Product Images from "Miltirone-induced apoptosis in cisplatin-resistant lung cancer cells through upregulation of p53 signaling pathways"

Article Title: Miltirone-induced apoptosis in cisplatin-resistant lung cancer cells through upregulation of p53 signaling pathways

Journal: Oncology Letters

doi: 10.3892/ol.2018.8440

Miltirone induces apoptosis in HCC827 and A549 cisplatin-resistant lung cancer cells. **P
Figure Legend Snippet: Miltirone induces apoptosis in HCC827 and A549 cisplatin-resistant lung cancer cells. **P

Techniques Used:

Miltirone decreases the viability of (A) HCC827 and (B) A549 cisplatin-resistant lung cancer cells. **P
Figure Legend Snippet: Miltirone decreases the viability of (A) HCC827 and (B) A549 cisplatin-resistant lung cancer cells. **P

Techniques Used:

Miltirone decreases mitochondrial ROS generation in HCC827 and A549 cisplatin-resistant lung cancer cells. **P
Figure Legend Snippet: Miltirone decreases mitochondrial ROS generation in HCC827 and A549 cisplatin-resistant lung cancer cells. **P

Techniques Used:

Miltirone induces Bax, AIF and p53 protein expression in HCC827 and A549 cisplatin-resistant lung cancer cells. (A) The effect of miltirone on Bax, AIF and p53 protein expression was determined using western blotting in HCC827 and A549 cisplatin-resistant lung cancer cells. The statistical analysis of (B) Bax, (C) AIF and (D) p53 protein expression. GAPDH served as a loading control. **P
Figure Legend Snippet: Miltirone induces Bax, AIF and p53 protein expression in HCC827 and A549 cisplatin-resistant lung cancer cells. (A) The effect of miltirone on Bax, AIF and p53 protein expression was determined using western blotting in HCC827 and A549 cisplatin-resistant lung cancer cells. The statistical analysis of (B) Bax, (C) AIF and (D) p53 protein expression. GAPDH served as a loading control. **P

Techniques Used: Expressing, Western Blot

Miltirone decreases MMP2/9 and increases PARP protein expression in HCC827 and A549 cisplatin-resistant lung cancer cells. (A) The effect of miltirone on MMP2/9 and PARP protein expression was determined using western blotting in HCC827 and A549 cisplatin-resistant lung cancer cells. The statistical analysis of (B) MMP2, (C) MMP9 and (D)PARP protein expression. GAPDH served as a loading control. **P
Figure Legend Snippet: Miltirone decreases MMP2/9 and increases PARP protein expression in HCC827 and A549 cisplatin-resistant lung cancer cells. (A) The effect of miltirone on MMP2/9 and PARP protein expression was determined using western blotting in HCC827 and A549 cisplatin-resistant lung cancer cells. The statistical analysis of (B) MMP2, (C) MMP9 and (D)PARP protein expression. GAPDH served as a loading control. **P

Techniques Used: Expressing, Western Blot

Miltirone decreases caspase-3/8 activity in (A) HCC827 and (B) A549 cisplatin-resistant lung cancer cells. **P
Figure Legend Snippet: Miltirone decreases caspase-3/8 activity in (A) HCC827 and (B) A549 cisplatin-resistant lung cancer cells. **P

Techniques Used: Activity Assay

9) Product Images from "Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo"

Article Title: Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S119226

( A ) Antitumor effect of CK and CK mixed micelles to A549 cell on different concentrations. * P
Figure Legend Snippet: ( A ) Antitumor effect of CK and CK mixed micelles to A549 cell on different concentrations. * P

Techniques Used:

Fluorescence microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.
Figure Legend Snippet: Fluorescence microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.

Techniques Used: Fluorescence, Microscopy

Confocal microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.
Figure Legend Snippet: Confocal microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.

Techniques Used: Confocal Microscopy

Cell cycle analysis assay (green G1, brown S, and blue G2 phase). ( A ) and wound healing assay ( B ) on A549 cell. Notes: n=3, mean ± SE. Scale 50 μm. Abbreviations: CK, compound K; SE, standard error.
Figure Legend Snippet: Cell cycle analysis assay (green G1, brown S, and blue G2 phase). ( A ) and wound healing assay ( B ) on A549 cell. Notes: n=3, mean ± SE. Scale 50 μm. Abbreviations: CK, compound K; SE, standard error.

Techniques Used: Cell Cycle Assay, Wound Healing Assay

10) Product Images from "Miltirone-induced apoptosis in cisplatin-resistant lung cancer cells through upregulation of p53 signaling pathways"

Article Title: Miltirone-induced apoptosis in cisplatin-resistant lung cancer cells through upregulation of p53 signaling pathways

Journal: Oncology Letters

doi: 10.3892/ol.2018.8440

Miltirone induces apoptosis in HCC827 and A549 cisplatin-resistant lung cancer cells. **P
Figure Legend Snippet: Miltirone induces apoptosis in HCC827 and A549 cisplatin-resistant lung cancer cells. **P

Techniques Used:

Miltirone decreases the viability of (A) HCC827 and (B) A549 cisplatin-resistant lung cancer cells. **P
Figure Legend Snippet: Miltirone decreases the viability of (A) HCC827 and (B) A549 cisplatin-resistant lung cancer cells. **P

Techniques Used:

Miltirone decreases mitochondrial ROS generation in HCC827 and A549 cisplatin-resistant lung cancer cells. **P
Figure Legend Snippet: Miltirone decreases mitochondrial ROS generation in HCC827 and A549 cisplatin-resistant lung cancer cells. **P

Techniques Used:

Miltirone induces Bax, AIF and p53 protein expression in HCC827 and A549 cisplatin-resistant lung cancer cells. (A) The effect of miltirone on Bax, AIF and p53 protein expression was determined using western blotting in HCC827 and A549 cisplatin-resistant lung cancer cells. The statistical analysis of (B) Bax, (C) AIF and (D) p53 protein expression. GAPDH served as a loading control. **P
Figure Legend Snippet: Miltirone induces Bax, AIF and p53 protein expression in HCC827 and A549 cisplatin-resistant lung cancer cells. (A) The effect of miltirone on Bax, AIF and p53 protein expression was determined using western blotting in HCC827 and A549 cisplatin-resistant lung cancer cells. The statistical analysis of (B) Bax, (C) AIF and (D) p53 protein expression. GAPDH served as a loading control. **P

Techniques Used: Expressing, Western Blot

Miltirone decreases MMP2/9 and increases PARP protein expression in HCC827 and A549 cisplatin-resistant lung cancer cells. (A) The effect of miltirone on MMP2/9 and PARP protein expression was determined using western blotting in HCC827 and A549 cisplatin-resistant lung cancer cells. The statistical analysis of (B) MMP2, (C) MMP9 and (D)PARP protein expression. GAPDH served as a loading control. **P
Figure Legend Snippet: Miltirone decreases MMP2/9 and increases PARP protein expression in HCC827 and A549 cisplatin-resistant lung cancer cells. (A) The effect of miltirone on MMP2/9 and PARP protein expression was determined using western blotting in HCC827 and A549 cisplatin-resistant lung cancer cells. The statistical analysis of (B) MMP2, (C) MMP9 and (D)PARP protein expression. GAPDH served as a loading control. **P

Techniques Used: Expressing, Western Blot

Miltirone decreases caspase-3/8 activity in (A) HCC827 and (B) A549 cisplatin-resistant lung cancer cells. **P
Figure Legend Snippet: Miltirone decreases caspase-3/8 activity in (A) HCC827 and (B) A549 cisplatin-resistant lung cancer cells. **P

Techniques Used: Activity Assay

11) Product Images from "Effect of phosphorylation and methylation on the function of the p16INK4a protein in non-small cell lung cancer A549 cells"

Article Title: Effect of phosphorylation and methylation on the function of the p16INK4a protein in non-small cell lung cancer A549 cells

Journal: Oncology Letters

doi: 10.3892/ol.2015.3617

Phosphorylation of the p16 protein in a variety of cell lines. Extracts from the A549, BT549, HeLa and 293T cell lines were prepared and precipitated with anti-p16 antibody, then detected on immunoblotting with antibodies against p16 or serine phosphorylation.
Figure Legend Snippet: Phosphorylation of the p16 protein in a variety of cell lines. Extracts from the A549, BT549, HeLa and 293T cell lines were prepared and precipitated with anti-p16 antibody, then detected on immunoblotting with antibodies against p16 or serine phosphorylation.

Techniques Used:

The p16 protein mutated at various serine residues exhibited a decreased ability to prevent the proliferation of A549 cells. (A) Immunoblotting of serine phosphorylation of p16 revealing that the mutation of residues 7, 8, 140 and 152 of p16 resulted
Figure Legend Snippet: The p16 protein mutated at various serine residues exhibited a decreased ability to prevent the proliferation of A549 cells. (A) Immunoblotting of serine phosphorylation of p16 revealing that the mutation of residues 7, 8, 140 and 152 of p16 resulted

Techniques Used: Mutagenesis

Crosstalk between the methylation of arginine and phosphorylation of serine in p16 in A549 cells. The cell extracts were prepared and precipitated with anti-p16 antibody, and then detected using immunoblotting with antibodies against GFP or serine phosphorylation.
Figure Legend Snippet: Crosstalk between the methylation of arginine and phosphorylation of serine in p16 in A549 cells. The cell extracts were prepared and precipitated with anti-p16 antibody, and then detected using immunoblotting with antibodies against GFP or serine phosphorylation.

Techniques Used: Methylation

The mutant p16KKK protein exhibited hypomethylation and enhanced function. (A) The arginine 22, 131 and 138 residues in the p16 protein were methylation sites. A549 cells transfected with the p16w and p16KKK expression vectors or control EGFP-N1 empty
Figure Legend Snippet: The mutant p16KKK protein exhibited hypomethylation and enhanced function. (A) The arginine 22, 131 and 138 residues in the p16 protein were methylation sites. A549 cells transfected with the p16w and p16KKK expression vectors or control EGFP-N1 empty

Techniques Used: Mutagenesis, Methylation, Transfection, Expressing

PRMT6 influences the functional properties of p16. (A) Western blot analysis of the expression of the PRMT1, PRMT4 and PRMT6 proteins in A549 cells transfected with the PRMT1, PRMT4 and PRMT6 expression vectors or the control pcDNA3.1 empty vector. (B)
Figure Legend Snippet: PRMT6 influences the functional properties of p16. (A) Western blot analysis of the expression of the PRMT1, PRMT4 and PRMT6 proteins in A549 cells transfected with the PRMT1, PRMT4 and PRMT6 expression vectors or the control pcDNA3.1 empty vector. (B)

Techniques Used: Functional Assay, Western Blot, Expressing, Transfection, Plasmid Preparation

12) Product Images from "Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo"

Article Title: Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S119226

( A ) Antitumor effect of CK and CK mixed micelles to A549 cell on different concentrations. * P
Figure Legend Snippet: ( A ) Antitumor effect of CK and CK mixed micelles to A549 cell on different concentrations. * P

Techniques Used:

Fluorescence microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.
Figure Legend Snippet: Fluorescence microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.

Techniques Used: Fluorescence, Microscopy

Confocal microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.
Figure Legend Snippet: Confocal microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.

Techniques Used: Confocal Microscopy

Cell cycle analysis assay (green G1, brown S, and blue G2 phase). ( A ) and wound healing assay ( B ) on A549 cell. Notes: n=3, mean ± SE. Scale 50 μm. Abbreviations: CK, compound K; SE, standard error.
Figure Legend Snippet: Cell cycle analysis assay (green G1, brown S, and blue G2 phase). ( A ) and wound healing assay ( B ) on A549 cell. Notes: n=3, mean ± SE. Scale 50 μm. Abbreviations: CK, compound K; SE, standard error.

Techniques Used: Cell Cycle Assay, Wound Healing Assay

13) Product Images from "Novel near-infrared fluorescent probe for live cell imaging"

Article Title: Novel near-infrared fluorescent probe for live cell imaging

Journal: Experimental and Therapeutic Medicine

doi: 10.3892/etm.2019.8323

Confocal fluorescence images of living A549 cells (magnification ×200). (A) Confocal fluorescence and (B) bright field images of living A549 cells incubated with 10 µM IR787 at 37°C for 30 min. (C) Confocal fluorescence and (D) bright field images of living A549 cells incubated with 10 µM ICG at 37°C for 30 min. ICG, indocyanine green.
Figure Legend Snippet: Confocal fluorescence images of living A549 cells (magnification ×200). (A) Confocal fluorescence and (B) bright field images of living A549 cells incubated with 10 µM IR787 at 37°C for 30 min. (C) Confocal fluorescence and (D) bright field images of living A549 cells incubated with 10 µM ICG at 37°C for 30 min. ICG, indocyanine green.

Techniques Used: Fluorescence, Incubation

Viability of A549 cells incubated with 0–120 µM IR787 and ICG. ICG, indocyanine green.
Figure Legend Snippet: Viability of A549 cells incubated with 0–120 µM IR787 and ICG. ICG, indocyanine green.

Techniques Used: Incubation

14) Product Images from "Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo"

Article Title: Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S119226

( A ) Antitumor effect of CK and CK mixed micelles to A549 cell on different concentrations. * P
Figure Legend Snippet: ( A ) Antitumor effect of CK and CK mixed micelles to A549 cell on different concentrations. * P

Techniques Used:

Fluorescence microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.
Figure Legend Snippet: Fluorescence microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.

Techniques Used: Fluorescence, Microscopy

Confocal microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.
Figure Legend Snippet: Confocal microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.

Techniques Used: Confocal Microscopy

Cell cycle analysis assay (green G1, brown S, and blue G2 phase). ( A ) and wound healing assay ( B ) on A549 cell. Notes: n=3, mean ± SE. Scale 50 μm. Abbreviations: CK, compound K; SE, standard error.
Figure Legend Snippet: Cell cycle analysis assay (green G1, brown S, and blue G2 phase). ( A ) and wound healing assay ( B ) on A549 cell. Notes: n=3, mean ± SE. Scale 50 μm. Abbreviations: CK, compound K; SE, standard error.

Techniques Used: Cell Cycle Assay, Wound Healing Assay

15) Product Images from "Combination treatment of gemcitabine and sorafenib exerts a synergistic inhibitory effect on non-small cell lung cancer in vitro and in vivo via the epithelial-to-mesenchymal transition process"

Article Title: Combination treatment of gemcitabine and sorafenib exerts a synergistic inhibitory effect on non-small cell lung cancer in vitro and in vivo via the epithelial-to-mesenchymal transition process

Journal: Oncology Letters

doi: 10.3892/ol.2020.11536

Co-treatment with gemcitabine and sorafenib induces apoptosis and cell cycle arrest in A549 cells. A549 cells were treated with 10 µg/ml gemcitabine and 10 µM sorafenib, either alone or in combination for 48 h. (A) Cell cycle distribution was determined by flow cytometry analysis. (B) The quantification of cells in different phases of the cell cycle is presented in bar charts. (C) The percentage of apoptotic cells was determined by flow cytometry analysis. PI, propidium iodide. (D) The percentage of A549 cells positive for Annexin V staining was presented in bar charts. *P
Figure Legend Snippet: Co-treatment with gemcitabine and sorafenib induces apoptosis and cell cycle arrest in A549 cells. A549 cells were treated with 10 µg/ml gemcitabine and 10 µM sorafenib, either alone or in combination for 48 h. (A) Cell cycle distribution was determined by flow cytometry analysis. (B) The quantification of cells in different phases of the cell cycle is presented in bar charts. (C) The percentage of apoptotic cells was determined by flow cytometry analysis. PI, propidium iodide. (D) The percentage of A549 cells positive for Annexin V staining was presented in bar charts. *P

Techniques Used: Flow Cytometry, Staining

Effects of co-treatment with gemcitabine and sorafenib on migration of A549 cells. (A) A549 cells were treated with a combination of 10 µg/ml gemcitabine and 10 µM sorafenib for 48 h, and cell migration was determined by a wound healing assay using an inverted microscope (magnification, ×200). (B) Quantification of A549 migratory cells. *P
Figure Legend Snippet: Effects of co-treatment with gemcitabine and sorafenib on migration of A549 cells. (A) A549 cells were treated with a combination of 10 µg/ml gemcitabine and 10 µM sorafenib for 48 h, and cell migration was determined by a wound healing assay using an inverted microscope (magnification, ×200). (B) Quantification of A549 migratory cells. *P

Techniques Used: Migration, Wound Healing Assay, Inverted Microscopy

Dose-dependent cytotoxic effect of gemcitabine and sorafenib on A549 cells. (A and B) The molecular structure of (A) gemcitabine and (B) sorafenib. (C and D) A549 cells were treated with (C) 0–100 µg/ml of gemcitabine and (D) 0–100 µM of sorafenib for 48 h, and cell viability was determined by an MTT assay. *P
Figure Legend Snippet: Dose-dependent cytotoxic effect of gemcitabine and sorafenib on A549 cells. (A and B) The molecular structure of (A) gemcitabine and (B) sorafenib. (C and D) A549 cells were treated with (C) 0–100 µg/ml of gemcitabine and (D) 0–100 µM of sorafenib for 48 h, and cell viability was determined by an MTT assay. *P

Techniques Used: MTT Assay

Synergistic antitumor effects of gemcitabine and sorafenib on A549 cells. (A) A549 cells were treated with 5 or 10 µg/ml gemcitabine and 5 or 10 µM sorafenib, either alone or in combination for 48 h, and cell viability was determined by an MTT assay. (B) A549 cells were treated with a combination of 10 µg/ml gemcitabine and 10 µM sorafenib for 48 h, and cell morphology was subsequently observed using an inverted microscope. (C) H1975 and (D) H1650 cells were treated with 10 µg/ml gemcitabine and 10 µM sorafenib, either alone or in combination for 48 h, and cell viability was determined by an MTT assay. *P
Figure Legend Snippet: Synergistic antitumor effects of gemcitabine and sorafenib on A549 cells. (A) A549 cells were treated with 5 or 10 µg/ml gemcitabine and 5 or 10 µM sorafenib, either alone or in combination for 48 h, and cell viability was determined by an MTT assay. (B) A549 cells were treated with a combination of 10 µg/ml gemcitabine and 10 µM sorafenib for 48 h, and cell morphology was subsequently observed using an inverted microscope. (C) H1975 and (D) H1650 cells were treated with 10 µg/ml gemcitabine and 10 µM sorafenib, either alone or in combination for 48 h, and cell viability was determined by an MTT assay. *P

Techniques Used: MTT Assay, Inverted Microscopy

Combination treatment with gemcitabine and sorafenib induces EMT inhibition of A549 cells. A549 cells were treated with a combination of 10 µg/ml gemcitabine and 10 µM sorafenib for 48 h. (A) Protein expression levels of EMT-associated markers N-cadherin, E-cadherin and Twist-1 were assessed by western blot analysis. (B-D) Quantitative analysis of (B) E-cadherin, (C) N-cadherin and (D) Twist-1. (E-G) mRNA expression levels of (E) E-cadherin, (F) N-cadherin and (G) Twist-1. *P
Figure Legend Snippet: Combination treatment with gemcitabine and sorafenib induces EMT inhibition of A549 cells. A549 cells were treated with a combination of 10 µg/ml gemcitabine and 10 µM sorafenib for 48 h. (A) Protein expression levels of EMT-associated markers N-cadherin, E-cadherin and Twist-1 were assessed by western blot analysis. (B-D) Quantitative analysis of (B) E-cadherin, (C) N-cadherin and (D) Twist-1. (E-G) mRNA expression levels of (E) E-cadherin, (F) N-cadherin and (G) Twist-1. *P

Techniques Used: Inhibition, Expressing, Western Blot

Effects of co-treatment with gemcitabine and sorafenib on invasion of A549 cells. (A) The result of cell invasion. (B) Quantification of A549 invasive cells. *P
Figure Legend Snippet: Effects of co-treatment with gemcitabine and sorafenib on invasion of A549 cells. (A) The result of cell invasion. (B) Quantification of A549 invasive cells. *P

Techniques Used:

Related Articles

Centrifugation:

Article Title: Combination treatment of gemcitabine and sorafenib exerts a synergistic inhibitory effect on non-small cell lung cancer in vitro and in vivo via the epithelial-to-mesenchymal transition process
Article Snippet: .. Flow cytometric analysis of apoptosis Following treatment with 10 µg/ml gemcitabine and 10 µM sorafenib at 37°C for 48 h, A549 cells were collected using centrifugation at 800 × g at 4°C for 10 min. Then, cells were stained with annexin V-fluorescein isothiocyanate (FITC) and PI using the Annexin V-FITC Apoptosis Detection kit (Nanjing KeyGen Biotech Co., Ltd.). .. Briefly, the cells were incubated with Annexin V-FITC/PI at 37°C for 20 min, and apoptotic cells were subsequently analyzed using a FACSCalibur flow cytometer (BD Biosciences); Annexin V+ /PI+ and Annexin V+ /PI− cells were considered to be apoptotic.

In Vitro:

Article Title: Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo
Article Snippet: .. In vitro cytotoxicity The results for in vitro cytotoxicity are shown in ; both free CK and CK mixed micelles have dose-dependent inhibitory effects on the viability of A549 cells. .. CK mixed micelles showed noticeable cytotoxicities in A549 tumor cells after 24 h incubation.

Protein Quantitation:

Article Title: Combination treatment of gemcitabine and sorafenib exerts a synergistic inhibitory effect on non-small cell lung cancer in vitro and in vivo via the epithelial-to-mesenchymal transition process
Article Snippet: .. Western blottingTotal protein was extracted from A549 cells and tumor tissue homogenates using RIPA cell lysis buffer (Nanjing KeyGen Biotech Co., Ltd.), and quantified using the BCA Protein Quantitation kit (Nanjing KeyGen Biotech Co., Ltd.). .. Equal amounts of total protein (20 µg) were separated by 12% SDS-PAGE and subsequently transferred to polyvinylidene difluoride membranes.

Cytometry:

Article Title: Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo
Article Snippet: .. Apoptosis in A549 cells Next, to further analyze apoptosis, flow cytometry was chosen to quantify the proportion of cells in early and late-stage apoptosis. ..

other:

Article Title: Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo
Article Snippet: After exposure to CK or CK mixed micelles at 10 μg/mL of CK for 24 h, A549 cells were harvested with trypsin–EDTA.

Article Title: Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo
Article Snippet: Briefly, after being treated with CK or CK mixed micelles, A549 cells were permeabilized by Tris-HCl and ethylenediaminetetraacetic acid (EDTA) for 10 min.

Staining:

Article Title: Combination treatment of gemcitabine and sorafenib exerts a synergistic inhibitory effect on non-small cell lung cancer in vitro and in vivo via the epithelial-to-mesenchymal transition process
Article Snippet: .. Flow cytometric analysis of apoptosis Following treatment with 10 µg/ml gemcitabine and 10 µM sorafenib at 37°C for 48 h, A549 cells were collected using centrifugation at 800 × g at 4°C for 10 min. Then, cells were stained with annexin V-fluorescein isothiocyanate (FITC) and PI using the Annexin V-FITC Apoptosis Detection kit (Nanjing KeyGen Biotech Co., Ltd.). .. Briefly, the cells were incubated with Annexin V-FITC/PI at 37°C for 20 min, and apoptotic cells were subsequently analyzed using a FACSCalibur flow cytometer (BD Biosciences); Annexin V+ /PI+ and Annexin V+ /PI− cells were considered to be apoptotic.

Western Blot:

Article Title: Combination treatment of gemcitabine and sorafenib exerts a synergistic inhibitory effect on non-small cell lung cancer in vitro and in vivo via the epithelial-to-mesenchymal transition process
Article Snippet: .. Western blottingTotal protein was extracted from A549 cells and tumor tissue homogenates using RIPA cell lysis buffer (Nanjing KeyGen Biotech Co., Ltd.), and quantified using the BCA Protein Quantitation kit (Nanjing KeyGen Biotech Co., Ltd.). .. Equal amounts of total protein (20 µg) were separated by 12% SDS-PAGE and subsequently transferred to polyvinylidene difluoride membranes.

Lysis:

Article Title: Combination treatment of gemcitabine and sorafenib exerts a synergistic inhibitory effect on non-small cell lung cancer in vitro and in vivo via the epithelial-to-mesenchymal transition process
Article Snippet: .. Western blottingTotal protein was extracted from A549 cells and tumor tissue homogenates using RIPA cell lysis buffer (Nanjing KeyGen Biotech Co., Ltd.), and quantified using the BCA Protein Quantitation kit (Nanjing KeyGen Biotech Co., Ltd.). .. Equal amounts of total protein (20 µg) were separated by 12% SDS-PAGE and subsequently transferred to polyvinylidene difluoride membranes.

Binding Assay:

Article Title: Miltirone-induced apoptosis in cisplatin-resistant lung cancer cells through upregulation of p53 signaling pathways
Article Snippet: .. HCC827 and A549 cells were seeded in duplicate in 6-well plates at a density of 2×105 cells per well and treated with 10, 20 or 40 µM miltirone for 48 h. HCC827 and A549 cells were then harvested and resuspended in 0.5 ml binding buffer containing Annexin V and propidium iodide (PI) (both from Nanjing KeyGen Biotech Co., Ltd., Nanjing, China) for 30 min at 37°C in the dark. .. Apoptotic cells were analyzed using a flow cytometer (BD FASCanto C6; BD Biosciences, Franklin Lakes, NJ, USA) and FlowJo 7.6.1. software (FlowJo, LLC, Ashland, OR, USA).

Flow Cytometry:

Article Title: Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo
Article Snippet: .. Apoptosis in A549 cells Next, to further analyze apoptosis, flow cytometry was chosen to quantify the proportion of cells in early and late-stage apoptosis. ..

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    Nanjing KeyGen Biotech Co Ltd ddp resistant lung cell line a549 ddp
    Propofol promoted the inhibitory effect of <t>DDP</t> to <t>A549</t> cell proliferation. We used 0, 10, 20, 40, 80, 160, and 320 μM DDP to treat A549 cells and then cultured in 5 μg/mL propofol or DMSO for 48 hours. MTT assay was conducted to determine cell proliferation ( A ) and measure the IC 50 value ( B ) in propofol-treated A549 cells. Results were exhibited as means±SD. * P
    Ddp Resistant Lung Cell Line A549 Ddp, supplied by Nanjing KeyGen Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ddp resistant lung cell line a549 ddp/product/Nanjing KeyGen Biotech Co Ltd
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ddp resistant lung cell line a549 ddp - by Bioz Stars, 2020-09
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    Nanjing KeyGen Biotech Co Ltd a549 cells
    ( A ) Antitumor effect of CK and CK mixed micelles to <t>A549</t> cell on different concentrations. * P
    A549 Cells, supplied by Nanjing KeyGen Biotech Co Ltd, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a549 cells/product/Nanjing KeyGen Biotech Co Ltd
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    a549 cells - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

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    Propofol promoted the inhibitory effect of DDP to A549 cell proliferation. We used 0, 10, 20, 40, 80, 160, and 320 μM DDP to treat A549 cells and then cultured in 5 μg/mL propofol or DMSO for 48 hours. MTT assay was conducted to determine cell proliferation ( A ) and measure the IC 50 value ( B ) in propofol-treated A549 cells. Results were exhibited as means±SD. * P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Propofol Improves Sensitivity of Lung Cancer Cells to Cisplatin and Its Mechanism

    doi: 10.12659/MSM.919786

    Figure Lengend Snippet: Propofol promoted the inhibitory effect of DDP to A549 cell proliferation. We used 0, 10, 20, 40, 80, 160, and 320 μM DDP to treat A549 cells and then cultured in 5 μg/mL propofol or DMSO for 48 hours. MTT assay was conducted to determine cell proliferation ( A ) and measure the IC 50 value ( B ) in propofol-treated A549 cells. Results were exhibited as means±SD. * P

    Article Snippet: Cell culture and drug treatment The human lung cancer cell line A549 and the DDP-resistant lung cell line A549/DDP were from Nanjing Keygen Biotech.

    Techniques: Cell Culture, MTT Assay

    Propofol promoted the suppression effect of DDP on A549/DDP cell proliferation. We used 0, 10, 20, 40, 80, 160, and 320 μM DDP to stimulate A549/DDP cells and then cultured in 5 μg/mL propofol or DMSO for 48 hours. The influence of propofol on cell growth in A549/DDP cells ( A ) were determined. MTT assay was conducted to evaluate the IC 50 value of DDP in A549/DDP ( B ) cells after propofol treatment. Results were exhibited as means±SD. * P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Propofol Improves Sensitivity of Lung Cancer Cells to Cisplatin and Its Mechanism

    doi: 10.12659/MSM.919786

    Figure Lengend Snippet: Propofol promoted the suppression effect of DDP on A549/DDP cell proliferation. We used 0, 10, 20, 40, 80, 160, and 320 μM DDP to stimulate A549/DDP cells and then cultured in 5 μg/mL propofol or DMSO for 48 hours. The influence of propofol on cell growth in A549/DDP cells ( A ) were determined. MTT assay was conducted to evaluate the IC 50 value of DDP in A549/DDP ( B ) cells after propofol treatment. Results were exhibited as means±SD. * P

    Article Snippet: Cell culture and drug treatment The human lung cancer cell line A549 and the DDP-resistant lung cell line A549/DDP were from Nanjing Keygen Biotech.

    Techniques: Cell Culture, MTT Assay

    DDP suppressed A549/DDP and A549 cells proliferation. The lung cancer cells were stimulated by various concentrations of DDP for 48 hours, and the A549/DDP and A549 cells proliferation suppression rates were determined by MTT assay. ( A ) The inhibitory effect of DDP on A549/DDP and A549 cell growth. ( B ) IC 50 value of DDP. The results were shown as means±SD. * P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Propofol Improves Sensitivity of Lung Cancer Cells to Cisplatin and Its Mechanism

    doi: 10.12659/MSM.919786

    Figure Lengend Snippet: DDP suppressed A549/DDP and A549 cells proliferation. The lung cancer cells were stimulated by various concentrations of DDP for 48 hours, and the A549/DDP and A549 cells proliferation suppression rates were determined by MTT assay. ( A ) The inhibitory effect of DDP on A549/DDP and A549 cell growth. ( B ) IC 50 value of DDP. The results were shown as means±SD. * P

    Article Snippet: Cell culture and drug treatment The human lung cancer cell line A549 and the DDP-resistant lung cell line A549/DDP were from Nanjing Keygen Biotech.

    Techniques: MTT Assay

    Effects of propofol on the Wnt/β-catenin signaling pathway in A549/DDP and A549 cells. A549/DDP and A549 cells were stimulated by 0, 1, 5, and 10 μg/mL propofol for 48 hours, then western blot assay and qRT-PCR were performed to assess protein and mRNA levels. ( A ) β-catenin and c-myc protein expressions were detected in A549/DDP cells. The mRNA levels of β-catenin ( B ) and c-myc ( C ) were evaluated by qRT-PCR in A549/DDP cells. ( D ) β-catenin and c-myc expressions were determined in A549 cells. qRT-PCR assay was used to test β-catenin ( E ) and c-myc ( F ) mRNA levels in A549 cells. Results were shown as the mean±SD. * P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Propofol Improves Sensitivity of Lung Cancer Cells to Cisplatin and Its Mechanism

    doi: 10.12659/MSM.919786

    Figure Lengend Snippet: Effects of propofol on the Wnt/β-catenin signaling pathway in A549/DDP and A549 cells. A549/DDP and A549 cells were stimulated by 0, 1, 5, and 10 μg/mL propofol for 48 hours, then western blot assay and qRT-PCR were performed to assess protein and mRNA levels. ( A ) β-catenin and c-myc protein expressions were detected in A549/DDP cells. The mRNA levels of β-catenin ( B ) and c-myc ( C ) were evaluated by qRT-PCR in A549/DDP cells. ( D ) β-catenin and c-myc expressions were determined in A549 cells. qRT-PCR assay was used to test β-catenin ( E ) and c-myc ( F ) mRNA levels in A549 cells. Results were shown as the mean±SD. * P

    Article Snippet: Cell culture and drug treatment The human lung cancer cell line A549 and the DDP-resistant lung cell line A549/DDP were from Nanjing Keygen Biotech.

    Techniques: Western Blot, Quantitative RT-PCR

    Propofol depressed A549/DDP cells growth and promoted apoptosis. We used 0, 1, 5, and 10 μg/mL propofol to induce A549/DDP cells for 48 hours. ( A ) Cell viability was evaluated by MTT assay. ( B ) Flow cytometry was carried out to determine cell apoptosis. ( C ) Apoptotic cell rate was calculated and presented. ( D ) The apoptosis-related proteins were assessed by western blot. These data were shown as means±SD. * P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Propofol Improves Sensitivity of Lung Cancer Cells to Cisplatin and Its Mechanism

    doi: 10.12659/MSM.919786

    Figure Lengend Snippet: Propofol depressed A549/DDP cells growth and promoted apoptosis. We used 0, 1, 5, and 10 μg/mL propofol to induce A549/DDP cells for 48 hours. ( A ) Cell viability was evaluated by MTT assay. ( B ) Flow cytometry was carried out to determine cell apoptosis. ( C ) Apoptotic cell rate was calculated and presented. ( D ) The apoptosis-related proteins were assessed by western blot. These data were shown as means±SD. * P

    Article Snippet: Cell culture and drug treatment The human lung cancer cell line A549 and the DDP-resistant lung cell line A549/DDP were from Nanjing Keygen Biotech.

    Techniques: MTT Assay, Flow Cytometry, Western Blot

    ( A ) Antitumor effect of CK and CK mixed micelles to A549 cell on different concentrations. * P

    Journal: International Journal of Nanomedicine

    Article Title: Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo

    doi: 10.2147/IJN.S119226

    Figure Lengend Snippet: ( A ) Antitumor effect of CK and CK mixed micelles to A549 cell on different concentrations. * P

    Article Snippet: Briefly, after being treated with CK or CK mixed micelles, A549 cells were permeabilized by Tris-HCl and ethylenediaminetetraacetic acid (EDTA) for 10 min.

    Techniques:

    Fluorescence microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.

    Journal: International Journal of Nanomedicine

    Article Title: Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo

    doi: 10.2147/IJN.S119226

    Figure Lengend Snippet: Fluorescence microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.

    Article Snippet: Briefly, after being treated with CK or CK mixed micelles, A549 cells were permeabilized by Tris-HCl and ethylenediaminetetraacetic acid (EDTA) for 10 min.

    Techniques: Fluorescence, Microscopy

    Confocal microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.

    Journal: International Journal of Nanomedicine

    Article Title: Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo

    doi: 10.2147/IJN.S119226

    Figure Lengend Snippet: Confocal microscopy of A549 cell following 4 h of treatment with C-6 or C-6 mixed micelles (n=3). Abbreviations: C-6, coumarin-6; DAPI, 4′,6-diamidino-2-phenylindole; h, hours.

    Article Snippet: Briefly, after being treated with CK or CK mixed micelles, A549 cells were permeabilized by Tris-HCl and ethylenediaminetetraacetic acid (EDTA) for 10 min.

    Techniques: Confocal Microscopy

    Cell cycle analysis assay (green G1, brown S, and blue G2 phase). ( A ) and wound healing assay ( B ) on A549 cell. Notes: n=3, mean ± SE. Scale 50 μm. Abbreviations: CK, compound K; SE, standard error.

    Journal: International Journal of Nanomedicine

    Article Title: Ascorbyl palmitate/d-α-tocopheryl polyethylene glycol 1000 succinate monoester mixed micelles for prolonged circulation and targeted delivery of compound K for antilung cancer therapy in vitro and in vivo

    doi: 10.2147/IJN.S119226

    Figure Lengend Snippet: Cell cycle analysis assay (green G1, brown S, and blue G2 phase). ( A ) and wound healing assay ( B ) on A549 cell. Notes: n=3, mean ± SE. Scale 50 μm. Abbreviations: CK, compound K; SE, standard error.

    Article Snippet: Briefly, after being treated with CK or CK mixed micelles, A549 cells were permeabilized by Tris-HCl and ethylenediaminetetraacetic acid (EDTA) for 10 min.

    Techniques: Cell Cycle Assay, Wound Healing Assay