a549 cells  (Aviva Systems)

 
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    Name:
    A549 Cell Lysate
    Description:
    A549 cell was lysed in RIPA buffer and mixed with equivalent volumes of 2X SDS PAGE loading buffer prior to lyophilization
    Catalog Number:
    AHL018
    Price:
    69
    Size:
    200ug
    Category:
    Cell Lysate
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    Structured Review

    Aviva Systems a549 cells
    Validation of ITGB3 as a direct target of miR-140-3p (A) Predicted binding sites of ITGB3 3’-UTR and miR-140-3p using an online tool TargetScan. The base pairing between the ITGB3 3’UTR binding site and the seed region of miR-140-3p is showed by vertical lines (top). The binding site of miR-140-3p and the mutated form of ITGB3 3’-UTR includes 3 nucleotides (bottom). (B) Relative luciferase activity levels following 24 hours transfection of <t>A549</t> cells with the Luciferase reporter vector containing the backbone vector only, ITGB3 3’-UTR (ITGB3wt) and the mutated form of the 3’-UTR (ITGB3mut), respectively. Data are presented as fold change of the mean + SEM, three individual experiments were undertaken in triplicate, ANOVA-test was used to assess significance with * p
    A549 cell was lysed in RIPA buffer and mixed with equivalent volumes of 2X SDS PAGE loading buffer prior to lyophilization
    https://www.bioz.com/result/a549 cells/product/Aviva Systems
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    a549 cells - by Bioz Stars, 2021-01
    92/100 stars

    Images

    1) Product Images from "Distinct mechanisms by which two forms of miR-140 suppress the malignant properties of lung cancer cells"

    Article Title: Distinct mechanisms by which two forms of miR-140 suppress the malignant properties of lung cancer cells

    Journal: Oncotarget

    doi: 10.18632/oncotarget.26356

    Validation of ITGB3 as a direct target of miR-140-3p (A) Predicted binding sites of ITGB3 3’-UTR and miR-140-3p using an online tool TargetScan. The base pairing between the ITGB3 3’UTR binding site and the seed region of miR-140-3p is showed by vertical lines (top). The binding site of miR-140-3p and the mutated form of ITGB3 3’-UTR includes 3 nucleotides (bottom). (B) Relative luciferase activity levels following 24 hours transfection of A549 cells with the Luciferase reporter vector containing the backbone vector only, ITGB3 3’-UTR (ITGB3wt) and the mutated form of the 3’-UTR (ITGB3mut), respectively. Data are presented as fold change of the mean + SEM, three individual experiments were undertaken in triplicate, ANOVA-test was used to assess significance with * p
    Figure Legend Snippet: Validation of ITGB3 as a direct target of miR-140-3p (A) Predicted binding sites of ITGB3 3’-UTR and miR-140-3p using an online tool TargetScan. The base pairing between the ITGB3 3’UTR binding site and the seed region of miR-140-3p is showed by vertical lines (top). The binding site of miR-140-3p and the mutated form of ITGB3 3’-UTR includes 3 nucleotides (bottom). (B) Relative luciferase activity levels following 24 hours transfection of A549 cells with the Luciferase reporter vector containing the backbone vector only, ITGB3 3’-UTR (ITGB3wt) and the mutated form of the 3’-UTR (ITGB3mut), respectively. Data are presented as fold change of the mean + SEM, three individual experiments were undertaken in triplicate, ANOVA-test was used to assess significance with * p

    Techniques Used: Binding Assay, Luciferase, Activity Assay, Transfection, Plasmid Preparation

    Change of miR expression and invasion properties in lung cancer cells after transfection with miR-140-3p mimic (A) Expression of miR-140-3p in A549 cells. (B) Expression of miR-140-3p in SK-MES-1 cells. (C) Transwell invasion property of A549 cells with response to miR-130-3p mimic. (D) Transwell invasion property of SK-MES-1 cells with response to miR-130-3p mimic. (E) Transwell migration property of A549 cells with response to miR-130-3p mimic. (F) Transwell migration property of SK-MES-1 cells with response to miR-130-3p mimic. Data are presented as mean + SEM, three individual experiments were undertaken in triplicate, Student's t -test was used to assess significance.
    Figure Legend Snippet: Change of miR expression and invasion properties in lung cancer cells after transfection with miR-140-3p mimic (A) Expression of miR-140-3p in A549 cells. (B) Expression of miR-140-3p in SK-MES-1 cells. (C) Transwell invasion property of A549 cells with response to miR-130-3p mimic. (D) Transwell invasion property of SK-MES-1 cells with response to miR-130-3p mimic. (E) Transwell migration property of A549 cells with response to miR-130-3p mimic. (F) Transwell migration property of SK-MES-1 cells with response to miR-130-3p mimic. Data are presented as mean + SEM, three individual experiments were undertaken in triplicate, Student's t -test was used to assess significance.

    Techniques Used: Expressing, Transfection, Migration

    The two strands of miR-140 have minor impact upon the metabolite profile of lung cancer cells Lung cancer cells (A549 and SK-MES-1) and BEAS-2B lung epithelial cells were treated with miRNA mimics for 24 hours before cell samples were collected for metabolite analysis using Mass spectrometry (GC-MS). (A) A principle component analysis of the metabolite data set for the three cell lines without treatment (NEG) and with either miRNA mimic. (B) Example GCMS chromatogram of m/z 174 representing glycine levels in the control (NEG, black line) and after treatment with miR-140-3P (red line) and miR-140-5P (blue line). (C) Normalized peak area of metabolites which differed in level in A549 cells dependent on treatment. (D) Normalized peak area of metabolites which differed in level dependent on the cell line studied. Data are presented as mean + SEM, experiments were undertaken in triplicate, T -test and Mann Whitney tests were used to assess significance among multiple groups with * p
    Figure Legend Snippet: The two strands of miR-140 have minor impact upon the metabolite profile of lung cancer cells Lung cancer cells (A549 and SK-MES-1) and BEAS-2B lung epithelial cells were treated with miRNA mimics for 24 hours before cell samples were collected for metabolite analysis using Mass spectrometry (GC-MS). (A) A principle component analysis of the metabolite data set for the three cell lines without treatment (NEG) and with either miRNA mimic. (B) Example GCMS chromatogram of m/z 174 representing glycine levels in the control (NEG, black line) and after treatment with miR-140-3P (red line) and miR-140-5P (blue line). (C) Normalized peak area of metabolites which differed in level in A549 cells dependent on treatment. (D) Normalized peak area of metabolites which differed in level dependent on the cell line studied. Data are presented as mean + SEM, experiments were undertaken in triplicate, T -test and Mann Whitney tests were used to assess significance among multiple groups with * p

    Techniques Used: Mass Spectrometry, Gas Chromatography-Mass Spectrometry, MANN-WHITNEY

    Tubule formation ability of the primary endothelial cells HUVECs co-cultured with TCM of lung cancer cells treated with miR-140-3p and 140-5p mimics HUVECs were seeded onto a layer of Matrigel with completed growth medium supplemented with 40% TCM from miR-treated lung cancer cells for 24 and 48 hours, respectively. The tubule formation was monitored in real-time by using the EVOS Cell Imaging System. Images were taken every 30 minutes for 6 hours. (A) Total length of the tubule structure form by HUVECs in the presence of TCM from A549. (B) Number of closed mesh structure formed by HUVECs in the presence of TCM from A549. (C) Total length of the tubule structure form by HUVECs in the presence of TCM from SK-MES-1. (D) Number of closed mesh structure formed by HUVECs in the presence of TCM from SK-MES-1. Data are presented as mean + SEM, three individual experiments were undertaken in triplicate, Kruskal-Wallis-test was used to assess significance with * p
    Figure Legend Snippet: Tubule formation ability of the primary endothelial cells HUVECs co-cultured with TCM of lung cancer cells treated with miR-140-3p and 140-5p mimics HUVECs were seeded onto a layer of Matrigel with completed growth medium supplemented with 40% TCM from miR-treated lung cancer cells for 24 and 48 hours, respectively. The tubule formation was monitored in real-time by using the EVOS Cell Imaging System. Images were taken every 30 minutes for 6 hours. (A) Total length of the tubule structure form by HUVECs in the presence of TCM from A549. (B) Number of closed mesh structure formed by HUVECs in the presence of TCM from A549. (C) Total length of the tubule structure form by HUVECs in the presence of TCM from SK-MES-1. (D) Number of closed mesh structure formed by HUVECs in the presence of TCM from SK-MES-1. Data are presented as mean + SEM, three individual experiments were undertaken in triplicate, Kruskal-Wallis-test was used to assess significance with * p

    Techniques Used: Cell Culture, Imaging

    2) Product Images from "Distinct mechanisms by which two forms of miR-140 suppress the malignant properties of lung cancer cells"

    Article Title: Distinct mechanisms by which two forms of miR-140 suppress the malignant properties of lung cancer cells

    Journal: Oncotarget

    doi: 10.18632/oncotarget.26356

    Validation of ITGB3 as a direct target of miR-140-3p (A) Predicted binding sites of ITGB3 3’-UTR and miR-140-3p using an online tool TargetScan. The base pairing between the ITGB3 3’UTR binding site and the seed region of miR-140-3p is showed by vertical lines (top). The binding site of miR-140-3p and the mutated form of ITGB3 3’-UTR includes 3 nucleotides (bottom). (B) Relative luciferase activity levels following 24 hours transfection of A549 cells with the Luciferase reporter vector containing the backbone vector only, ITGB3 3’-UTR (ITGB3wt) and the mutated form of the 3’-UTR (ITGB3mut), respectively. Data are presented as fold change of the mean + SEM, three individual experiments were undertaken in triplicate, ANOVA-test was used to assess significance with * p
    Figure Legend Snippet: Validation of ITGB3 as a direct target of miR-140-3p (A) Predicted binding sites of ITGB3 3’-UTR and miR-140-3p using an online tool TargetScan. The base pairing between the ITGB3 3’UTR binding site and the seed region of miR-140-3p is showed by vertical lines (top). The binding site of miR-140-3p and the mutated form of ITGB3 3’-UTR includes 3 nucleotides (bottom). (B) Relative luciferase activity levels following 24 hours transfection of A549 cells with the Luciferase reporter vector containing the backbone vector only, ITGB3 3’-UTR (ITGB3wt) and the mutated form of the 3’-UTR (ITGB3mut), respectively. Data are presented as fold change of the mean + SEM, three individual experiments were undertaken in triplicate, ANOVA-test was used to assess significance with * p

    Techniques Used: Binding Assay, Luciferase, Activity Assay, Transfection, Plasmid Preparation

    Change of miR expression and invasion properties in lung cancer cells after transfection with miR-140-3p mimic (A) Expression of miR-140-3p in A549 cells. (B) Expression of miR-140-3p in SK-MES-1 cells. (C) Transwell invasion property of A549 cells with response to miR-130-3p mimic. (D) Transwell invasion property of SK-MES-1 cells with response to miR-130-3p mimic. (E) Transwell migration property of A549 cells with response to miR-130-3p mimic. (F) Transwell migration property of SK-MES-1 cells with response to miR-130-3p mimic. Data are presented as mean + SEM, three individual experiments were undertaken in triplicate, Student's t -test was used to assess significance.
    Figure Legend Snippet: Change of miR expression and invasion properties in lung cancer cells after transfection with miR-140-3p mimic (A) Expression of miR-140-3p in A549 cells. (B) Expression of miR-140-3p in SK-MES-1 cells. (C) Transwell invasion property of A549 cells with response to miR-130-3p mimic. (D) Transwell invasion property of SK-MES-1 cells with response to miR-130-3p mimic. (E) Transwell migration property of A549 cells with response to miR-130-3p mimic. (F) Transwell migration property of SK-MES-1 cells with response to miR-130-3p mimic. Data are presented as mean + SEM, three individual experiments were undertaken in triplicate, Student's t -test was used to assess significance.

    Techniques Used: Expressing, Transfection, Migration

    The two strands of miR-140 have minor impact upon the metabolite profile of lung cancer cells Lung cancer cells (A549 and SK-MES-1) and BEAS-2B lung epithelial cells were treated with miRNA mimics for 24 hours before cell samples were collected for metabolite analysis using Mass spectrometry (GC-MS). (A) A principle component analysis of the metabolite data set for the three cell lines without treatment (NEG) and with either miRNA mimic. (B) Example GCMS chromatogram of m/z 174 representing glycine levels in the control (NEG, black line) and after treatment with miR-140-3P (red line) and miR-140-5P (blue line). (C) Normalized peak area of metabolites which differed in level in A549 cells dependent on treatment. (D) Normalized peak area of metabolites which differed in level dependent on the cell line studied. Data are presented as mean + SEM, experiments were undertaken in triplicate, T -test and Mann Whitney tests were used to assess significance among multiple groups with * p
    Figure Legend Snippet: The two strands of miR-140 have minor impact upon the metabolite profile of lung cancer cells Lung cancer cells (A549 and SK-MES-1) and BEAS-2B lung epithelial cells were treated with miRNA mimics for 24 hours before cell samples were collected for metabolite analysis using Mass spectrometry (GC-MS). (A) A principle component analysis of the metabolite data set for the three cell lines without treatment (NEG) and with either miRNA mimic. (B) Example GCMS chromatogram of m/z 174 representing glycine levels in the control (NEG, black line) and after treatment with miR-140-3P (red line) and miR-140-5P (blue line). (C) Normalized peak area of metabolites which differed in level in A549 cells dependent on treatment. (D) Normalized peak area of metabolites which differed in level dependent on the cell line studied. Data are presented as mean + SEM, experiments were undertaken in triplicate, T -test and Mann Whitney tests were used to assess significance among multiple groups with * p

    Techniques Used: Mass Spectrometry, Gas Chromatography-Mass Spectrometry, MANN-WHITNEY

    Tubule formation ability of the primary endothelial cells HUVECs co-cultured with TCM of lung cancer cells treated with miR-140-3p and 140-5p mimics HUVECs were seeded onto a layer of Matrigel with completed growth medium supplemented with 40% TCM from miR-treated lung cancer cells for 24 and 48 hours, respectively. The tubule formation was monitored in real-time by using the EVOS Cell Imaging System. Images were taken every 30 minutes for 6 hours. (A) Total length of the tubule structure form by HUVECs in the presence of TCM from A549. (B) Number of closed mesh structure formed by HUVECs in the presence of TCM from A549. (C) Total length of the tubule structure form by HUVECs in the presence of TCM from SK-MES-1. (D) Number of closed mesh structure formed by HUVECs in the presence of TCM from SK-MES-1. Data are presented as mean + SEM, three individual experiments were undertaken in triplicate, Kruskal-Wallis-test was used to assess significance with * p
    Figure Legend Snippet: Tubule formation ability of the primary endothelial cells HUVECs co-cultured with TCM of lung cancer cells treated with miR-140-3p and 140-5p mimics HUVECs were seeded onto a layer of Matrigel with completed growth medium supplemented with 40% TCM from miR-treated lung cancer cells for 24 and 48 hours, respectively. The tubule formation was monitored in real-time by using the EVOS Cell Imaging System. Images were taken every 30 minutes for 6 hours. (A) Total length of the tubule structure form by HUVECs in the presence of TCM from A549. (B) Number of closed mesh structure formed by HUVECs in the presence of TCM from A549. (C) Total length of the tubule structure form by HUVECs in the presence of TCM from SK-MES-1. (D) Number of closed mesh structure formed by HUVECs in the presence of TCM from SK-MES-1. Data are presented as mean + SEM, three individual experiments were undertaken in triplicate, Kruskal-Wallis-test was used to assess significance with * p

    Techniques Used: Cell Culture, Imaging

    Distinctive gene regulation profiles and cell signalling pathways induced by two strands of miR-140 (A) Heatmap of the RNA-seq data showing the altered upregulated (red) and downregulated (green) genes in A549 treated with miR-140-3p and miR-140-5p, respectively. Global transcriptome analysis of 20816 gene targets was performed using the Ion AmpliSeq™ human gene expression panel. (B) The Venn diagram showing the differential gene expression induced by miR-140-3p and miR-140-5p, respectively, based on the RNA-Seq data. (C) Heatmap of the Kinex antibody array data showing the distinctive cell signalling pathways altered by the two strands of miR-140. The values of Z-score reflected positive or negative shifts in differential protein expression fold-changes after normalization by protein concentration and negative miRNA mimics. (D) Evaluation the expression of some key downstream effectors of miR-140-3p in A549 cells after treatments for 48 hours by Western blotting.
    Figure Legend Snippet: Distinctive gene regulation profiles and cell signalling pathways induced by two strands of miR-140 (A) Heatmap of the RNA-seq data showing the altered upregulated (red) and downregulated (green) genes in A549 treated with miR-140-3p and miR-140-5p, respectively. Global transcriptome analysis of 20816 gene targets was performed using the Ion AmpliSeq™ human gene expression panel. (B) The Venn diagram showing the differential gene expression induced by miR-140-3p and miR-140-5p, respectively, based on the RNA-Seq data. (C) Heatmap of the Kinex antibody array data showing the distinctive cell signalling pathways altered by the two strands of miR-140. The values of Z-score reflected positive or negative shifts in differential protein expression fold-changes after normalization by protein concentration and negative miRNA mimics. (D) Evaluation the expression of some key downstream effectors of miR-140-3p in A549 cells after treatments for 48 hours by Western blotting.

    Techniques Used: RNA Sequencing Assay, Expressing, Ab Array, Protein Concentration, Western Blot

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Distinct mechanisms by which two forms of miR-140 suppress the malignant properties of lung cancer cells
    Article Snippet: .. Enzyme linked immunosorbent assay (ELISA) The human vascular endothelial growth factor A (VEGF-A) levels in the TCM of A549 cells transfected with negative and miR-140-3p mimics were quantified by using 96-well plates pre-coated with VEGF-A primary antibody by using a specific kit purchased from Aviva System Biology, San Diego, USA. ..

    Transfection:

    Article Title: Distinct mechanisms by which two forms of miR-140 suppress the malignant properties of lung cancer cells
    Article Snippet: .. Enzyme linked immunosorbent assay (ELISA) The human vascular endothelial growth factor A (VEGF-A) levels in the TCM of A549 cells transfected with negative and miR-140-3p mimics were quantified by using 96-well plates pre-coated with VEGF-A primary antibody by using a specific kit purchased from Aviva System Biology, San Diego, USA. ..

    Article Title: Distinct mechanisms by which two forms of miR-140 suppress the malignant properties of lung cancer cells
    Article Snippet: .. The human vascular endothelial growth factor A (VEGF-A) levels in the TCM of A549 cells transfected with negative and miR-140-3p mimics were quantified by using 96-well plates pre-coated with VEGF-A primary antibody by using a specific kit purchased from Aviva System Biology, San Diego, USA. ..

    Incubation:

    Article Title: KCTD10 interacts with proliferating cell nuclear antigen and its down-regulation could inhibit cell proliferation.
    Article Snippet: .. A novel gene (GenBank accession No. AF113208) named KCTD10 (potassium channel tetramerisation domain-containing 10) was cloned from our 5300 EST database of human aorta cDNA library. .. A novel gene (GenBank accession No. AF113208) named KCTD10 (potassium channel tetramerisation domain-containing 10) was cloned from our 5300 EST database of human aorta cDNA library.

    Blocking Assay:

    Article Title: KCTD10 interacts with proliferating cell nuclear antigen and its down-regulation could inhibit cell proliferation.
    Article Snippet: .. A novel gene (GenBank accession No. AF113208) named KCTD10 (potassium channel tetramerisation domain-containing 10) was cloned from our 5300 EST database of human aorta cDNA library. .. A novel gene (GenBank accession No. AF113208) named KCTD10 (potassium channel tetramerisation domain-containing 10) was cloned from our 5300 EST database of human aorta cDNA library.

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  • 92
    Aviva Systems a549 cells
    Validation of ITGB3 as a direct target of miR-140-3p (A) Predicted binding sites of ITGB3 3’-UTR and miR-140-3p using an online tool TargetScan. The base pairing between the ITGB3 3’UTR binding site and the seed region of miR-140-3p is showed by vertical lines (top). The binding site of miR-140-3p and the mutated form of ITGB3 3’-UTR includes 3 nucleotides (bottom). (B) Relative luciferase activity levels following 24 hours transfection of <t>A549</t> cells with the Luciferase reporter vector containing the backbone vector only, ITGB3 3’-UTR (ITGB3wt) and the mutated form of the 3’-UTR (ITGB3mut), respectively. Data are presented as fold change of the mean + SEM, three individual experiments were undertaken in triplicate, ANOVA-test was used to assess significance with * p
    A549 Cells, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a549 cells/product/Aviva Systems
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    a549 cells - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

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    Ready to use whole cell lysates produced by We are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality protein integrity and lot
      Buy from Supplier

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    A549 cell was lysed in RIPA buffer and mixed with equivalent volumes of 2X SDS PAGE loading buffer prior to lyophilization
      Buy from Supplier

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    Human lung carcinoma
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    Image Search Results


    Validation of ITGB3 as a direct target of miR-140-3p (A) Predicted binding sites of ITGB3 3’-UTR and miR-140-3p using an online tool TargetScan. The base pairing between the ITGB3 3’UTR binding site and the seed region of miR-140-3p is showed by vertical lines (top). The binding site of miR-140-3p and the mutated form of ITGB3 3’-UTR includes 3 nucleotides (bottom). (B) Relative luciferase activity levels following 24 hours transfection of A549 cells with the Luciferase reporter vector containing the backbone vector only, ITGB3 3’-UTR (ITGB3wt) and the mutated form of the 3’-UTR (ITGB3mut), respectively. Data are presented as fold change of the mean + SEM, three individual experiments were undertaken in triplicate, ANOVA-test was used to assess significance with * p

    Journal: Oncotarget

    Article Title: Distinct mechanisms by which two forms of miR-140 suppress the malignant properties of lung cancer cells

    doi: 10.18632/oncotarget.26356

    Figure Lengend Snippet: Validation of ITGB3 as a direct target of miR-140-3p (A) Predicted binding sites of ITGB3 3’-UTR and miR-140-3p using an online tool TargetScan. The base pairing between the ITGB3 3’UTR binding site and the seed region of miR-140-3p is showed by vertical lines (top). The binding site of miR-140-3p and the mutated form of ITGB3 3’-UTR includes 3 nucleotides (bottom). (B) Relative luciferase activity levels following 24 hours transfection of A549 cells with the Luciferase reporter vector containing the backbone vector only, ITGB3 3’-UTR (ITGB3wt) and the mutated form of the 3’-UTR (ITGB3mut), respectively. Data are presented as fold change of the mean + SEM, three individual experiments were undertaken in triplicate, ANOVA-test was used to assess significance with * p

    Article Snippet: The human vascular endothelial growth factor A (VEGF-A) levels in the TCM of A549 cells transfected with negative and miR-140-3p mimics were quantified by using 96-well plates pre-coated with VEGF-A primary antibody by using a specific kit purchased from Aviva System Biology, San Diego, USA.

    Techniques: Binding Assay, Luciferase, Activity Assay, Transfection, Plasmid Preparation

    Change of miR expression and invasion properties in lung cancer cells after transfection with miR-140-3p mimic (A) Expression of miR-140-3p in A549 cells. (B) Expression of miR-140-3p in SK-MES-1 cells. (C) Transwell invasion property of A549 cells with response to miR-130-3p mimic. (D) Transwell invasion property of SK-MES-1 cells with response to miR-130-3p mimic. (E) Transwell migration property of A549 cells with response to miR-130-3p mimic. (F) Transwell migration property of SK-MES-1 cells with response to miR-130-3p mimic. Data are presented as mean + SEM, three individual experiments were undertaken in triplicate, Student's t -test was used to assess significance.

    Journal: Oncotarget

    Article Title: Distinct mechanisms by which two forms of miR-140 suppress the malignant properties of lung cancer cells

    doi: 10.18632/oncotarget.26356

    Figure Lengend Snippet: Change of miR expression and invasion properties in lung cancer cells after transfection with miR-140-3p mimic (A) Expression of miR-140-3p in A549 cells. (B) Expression of miR-140-3p in SK-MES-1 cells. (C) Transwell invasion property of A549 cells with response to miR-130-3p mimic. (D) Transwell invasion property of SK-MES-1 cells with response to miR-130-3p mimic. (E) Transwell migration property of A549 cells with response to miR-130-3p mimic. (F) Transwell migration property of SK-MES-1 cells with response to miR-130-3p mimic. Data are presented as mean + SEM, three individual experiments were undertaken in triplicate, Student's t -test was used to assess significance.

    Article Snippet: The human vascular endothelial growth factor A (VEGF-A) levels in the TCM of A549 cells transfected with negative and miR-140-3p mimics were quantified by using 96-well plates pre-coated with VEGF-A primary antibody by using a specific kit purchased from Aviva System Biology, San Diego, USA.

    Techniques: Expressing, Transfection, Migration

    The two strands of miR-140 have minor impact upon the metabolite profile of lung cancer cells Lung cancer cells (A549 and SK-MES-1) and BEAS-2B lung epithelial cells were treated with miRNA mimics for 24 hours before cell samples were collected for metabolite analysis using Mass spectrometry (GC-MS). (A) A principle component analysis of the metabolite data set for the three cell lines without treatment (NEG) and with either miRNA mimic. (B) Example GCMS chromatogram of m/z 174 representing glycine levels in the control (NEG, black line) and after treatment with miR-140-3P (red line) and miR-140-5P (blue line). (C) Normalized peak area of metabolites which differed in level in A549 cells dependent on treatment. (D) Normalized peak area of metabolites which differed in level dependent on the cell line studied. Data are presented as mean + SEM, experiments were undertaken in triplicate, T -test and Mann Whitney tests were used to assess significance among multiple groups with * p

    Journal: Oncotarget

    Article Title: Distinct mechanisms by which two forms of miR-140 suppress the malignant properties of lung cancer cells

    doi: 10.18632/oncotarget.26356

    Figure Lengend Snippet: The two strands of miR-140 have minor impact upon the metabolite profile of lung cancer cells Lung cancer cells (A549 and SK-MES-1) and BEAS-2B lung epithelial cells were treated with miRNA mimics for 24 hours before cell samples were collected for metabolite analysis using Mass spectrometry (GC-MS). (A) A principle component analysis of the metabolite data set for the three cell lines without treatment (NEG) and with either miRNA mimic. (B) Example GCMS chromatogram of m/z 174 representing glycine levels in the control (NEG, black line) and after treatment with miR-140-3P (red line) and miR-140-5P (blue line). (C) Normalized peak area of metabolites which differed in level in A549 cells dependent on treatment. (D) Normalized peak area of metabolites which differed in level dependent on the cell line studied. Data are presented as mean + SEM, experiments were undertaken in triplicate, T -test and Mann Whitney tests were used to assess significance among multiple groups with * p

    Article Snippet: The human vascular endothelial growth factor A (VEGF-A) levels in the TCM of A549 cells transfected with negative and miR-140-3p mimics were quantified by using 96-well plates pre-coated with VEGF-A primary antibody by using a specific kit purchased from Aviva System Biology, San Diego, USA.

    Techniques: Mass Spectrometry, Gas Chromatography-Mass Spectrometry, MANN-WHITNEY

    Tubule formation ability of the primary endothelial cells HUVECs co-cultured with TCM of lung cancer cells treated with miR-140-3p and 140-5p mimics HUVECs were seeded onto a layer of Matrigel with completed growth medium supplemented with 40% TCM from miR-treated lung cancer cells for 24 and 48 hours, respectively. The tubule formation was monitored in real-time by using the EVOS Cell Imaging System. Images were taken every 30 minutes for 6 hours. (A) Total length of the tubule structure form by HUVECs in the presence of TCM from A549. (B) Number of closed mesh structure formed by HUVECs in the presence of TCM from A549. (C) Total length of the tubule structure form by HUVECs in the presence of TCM from SK-MES-1. (D) Number of closed mesh structure formed by HUVECs in the presence of TCM from SK-MES-1. Data are presented as mean + SEM, three individual experiments were undertaken in triplicate, Kruskal-Wallis-test was used to assess significance with * p

    Journal: Oncotarget

    Article Title: Distinct mechanisms by which two forms of miR-140 suppress the malignant properties of lung cancer cells

    doi: 10.18632/oncotarget.26356

    Figure Lengend Snippet: Tubule formation ability of the primary endothelial cells HUVECs co-cultured with TCM of lung cancer cells treated with miR-140-3p and 140-5p mimics HUVECs were seeded onto a layer of Matrigel with completed growth medium supplemented with 40% TCM from miR-treated lung cancer cells for 24 and 48 hours, respectively. The tubule formation was monitored in real-time by using the EVOS Cell Imaging System. Images were taken every 30 minutes for 6 hours. (A) Total length of the tubule structure form by HUVECs in the presence of TCM from A549. (B) Number of closed mesh structure formed by HUVECs in the presence of TCM from A549. (C) Total length of the tubule structure form by HUVECs in the presence of TCM from SK-MES-1. (D) Number of closed mesh structure formed by HUVECs in the presence of TCM from SK-MES-1. Data are presented as mean + SEM, three individual experiments were undertaken in triplicate, Kruskal-Wallis-test was used to assess significance with * p

    Article Snippet: The human vascular endothelial growth factor A (VEGF-A) levels in the TCM of A549 cells transfected with negative and miR-140-3p mimics were quantified by using 96-well plates pre-coated with VEGF-A primary antibody by using a specific kit purchased from Aviva System Biology, San Diego, USA.

    Techniques: Cell Culture, Imaging

    Validation of ITGB3 as a direct target of miR-140-3p (A) Predicted binding sites of ITGB3 3’-UTR and miR-140-3p using an online tool TargetScan. The base pairing between the ITGB3 3’UTR binding site and the seed region of miR-140-3p is showed by vertical lines (top). The binding site of miR-140-3p and the mutated form of ITGB3 3’-UTR includes 3 nucleotides (bottom). (B) Relative luciferase activity levels following 24 hours transfection of A549 cells with the Luciferase reporter vector containing the backbone vector only, ITGB3 3’-UTR (ITGB3wt) and the mutated form of the 3’-UTR (ITGB3mut), respectively. Data are presented as fold change of the mean + SEM, three individual experiments were undertaken in triplicate, ANOVA-test was used to assess significance with * p

    Journal: Oncotarget

    Article Title: Distinct mechanisms by which two forms of miR-140 suppress the malignant properties of lung cancer cells

    doi: 10.18632/oncotarget.26356

    Figure Lengend Snippet: Validation of ITGB3 as a direct target of miR-140-3p (A) Predicted binding sites of ITGB3 3’-UTR and miR-140-3p using an online tool TargetScan. The base pairing between the ITGB3 3’UTR binding site and the seed region of miR-140-3p is showed by vertical lines (top). The binding site of miR-140-3p and the mutated form of ITGB3 3’-UTR includes 3 nucleotides (bottom). (B) Relative luciferase activity levels following 24 hours transfection of A549 cells with the Luciferase reporter vector containing the backbone vector only, ITGB3 3’-UTR (ITGB3wt) and the mutated form of the 3’-UTR (ITGB3mut), respectively. Data are presented as fold change of the mean + SEM, three individual experiments were undertaken in triplicate, ANOVA-test was used to assess significance with * p

    Article Snippet: Enzyme linked immunosorbent assay (ELISA) The human vascular endothelial growth factor A (VEGF-A) levels in the TCM of A549 cells transfected with negative and miR-140-3p mimics were quantified by using 96-well plates pre-coated with VEGF-A primary antibody by using a specific kit purchased from Aviva System Biology, San Diego, USA.

    Techniques: Binding Assay, Luciferase, Activity Assay, Transfection, Plasmid Preparation

    Change of miR expression and invasion properties in lung cancer cells after transfection with miR-140-3p mimic (A) Expression of miR-140-3p in A549 cells. (B) Expression of miR-140-3p in SK-MES-1 cells. (C) Transwell invasion property of A549 cells with response to miR-130-3p mimic. (D) Transwell invasion property of SK-MES-1 cells with response to miR-130-3p mimic. (E) Transwell migration property of A549 cells with response to miR-130-3p mimic. (F) Transwell migration property of SK-MES-1 cells with response to miR-130-3p mimic. Data are presented as mean + SEM, three individual experiments were undertaken in triplicate, Student's t -test was used to assess significance.

    Journal: Oncotarget

    Article Title: Distinct mechanisms by which two forms of miR-140 suppress the malignant properties of lung cancer cells

    doi: 10.18632/oncotarget.26356

    Figure Lengend Snippet: Change of miR expression and invasion properties in lung cancer cells after transfection with miR-140-3p mimic (A) Expression of miR-140-3p in A549 cells. (B) Expression of miR-140-3p in SK-MES-1 cells. (C) Transwell invasion property of A549 cells with response to miR-130-3p mimic. (D) Transwell invasion property of SK-MES-1 cells with response to miR-130-3p mimic. (E) Transwell migration property of A549 cells with response to miR-130-3p mimic. (F) Transwell migration property of SK-MES-1 cells with response to miR-130-3p mimic. Data are presented as mean + SEM, three individual experiments were undertaken in triplicate, Student's t -test was used to assess significance.

    Article Snippet: Enzyme linked immunosorbent assay (ELISA) The human vascular endothelial growth factor A (VEGF-A) levels in the TCM of A549 cells transfected with negative and miR-140-3p mimics were quantified by using 96-well plates pre-coated with VEGF-A primary antibody by using a specific kit purchased from Aviva System Biology, San Diego, USA.

    Techniques: Expressing, Transfection, Migration

    The two strands of miR-140 have minor impact upon the metabolite profile of lung cancer cells Lung cancer cells (A549 and SK-MES-1) and BEAS-2B lung epithelial cells were treated with miRNA mimics for 24 hours before cell samples were collected for metabolite analysis using Mass spectrometry (GC-MS). (A) A principle component analysis of the metabolite data set for the three cell lines without treatment (NEG) and with either miRNA mimic. (B) Example GCMS chromatogram of m/z 174 representing glycine levels in the control (NEG, black line) and after treatment with miR-140-3P (red line) and miR-140-5P (blue line). (C) Normalized peak area of metabolites which differed in level in A549 cells dependent on treatment. (D) Normalized peak area of metabolites which differed in level dependent on the cell line studied. Data are presented as mean + SEM, experiments were undertaken in triplicate, T -test and Mann Whitney tests were used to assess significance among multiple groups with * p

    Journal: Oncotarget

    Article Title: Distinct mechanisms by which two forms of miR-140 suppress the malignant properties of lung cancer cells

    doi: 10.18632/oncotarget.26356

    Figure Lengend Snippet: The two strands of miR-140 have minor impact upon the metabolite profile of lung cancer cells Lung cancer cells (A549 and SK-MES-1) and BEAS-2B lung epithelial cells were treated with miRNA mimics for 24 hours before cell samples were collected for metabolite analysis using Mass spectrometry (GC-MS). (A) A principle component analysis of the metabolite data set for the three cell lines without treatment (NEG) and with either miRNA mimic. (B) Example GCMS chromatogram of m/z 174 representing glycine levels in the control (NEG, black line) and after treatment with miR-140-3P (red line) and miR-140-5P (blue line). (C) Normalized peak area of metabolites which differed in level in A549 cells dependent on treatment. (D) Normalized peak area of metabolites which differed in level dependent on the cell line studied. Data are presented as mean + SEM, experiments were undertaken in triplicate, T -test and Mann Whitney tests were used to assess significance among multiple groups with * p

    Article Snippet: Enzyme linked immunosorbent assay (ELISA) The human vascular endothelial growth factor A (VEGF-A) levels in the TCM of A549 cells transfected with negative and miR-140-3p mimics were quantified by using 96-well plates pre-coated with VEGF-A primary antibody by using a specific kit purchased from Aviva System Biology, San Diego, USA.

    Techniques: Mass Spectrometry, Gas Chromatography-Mass Spectrometry, MANN-WHITNEY

    Tubule formation ability of the primary endothelial cells HUVECs co-cultured with TCM of lung cancer cells treated with miR-140-3p and 140-5p mimics HUVECs were seeded onto a layer of Matrigel with completed growth medium supplemented with 40% TCM from miR-treated lung cancer cells for 24 and 48 hours, respectively. The tubule formation was monitored in real-time by using the EVOS Cell Imaging System. Images were taken every 30 minutes for 6 hours. (A) Total length of the tubule structure form by HUVECs in the presence of TCM from A549. (B) Number of closed mesh structure formed by HUVECs in the presence of TCM from A549. (C) Total length of the tubule structure form by HUVECs in the presence of TCM from SK-MES-1. (D) Number of closed mesh structure formed by HUVECs in the presence of TCM from SK-MES-1. Data are presented as mean + SEM, three individual experiments were undertaken in triplicate, Kruskal-Wallis-test was used to assess significance with * p

    Journal: Oncotarget

    Article Title: Distinct mechanisms by which two forms of miR-140 suppress the malignant properties of lung cancer cells

    doi: 10.18632/oncotarget.26356

    Figure Lengend Snippet: Tubule formation ability of the primary endothelial cells HUVECs co-cultured with TCM of lung cancer cells treated with miR-140-3p and 140-5p mimics HUVECs were seeded onto a layer of Matrigel with completed growth medium supplemented with 40% TCM from miR-treated lung cancer cells for 24 and 48 hours, respectively. The tubule formation was monitored in real-time by using the EVOS Cell Imaging System. Images were taken every 30 minutes for 6 hours. (A) Total length of the tubule structure form by HUVECs in the presence of TCM from A549. (B) Number of closed mesh structure formed by HUVECs in the presence of TCM from A549. (C) Total length of the tubule structure form by HUVECs in the presence of TCM from SK-MES-1. (D) Number of closed mesh structure formed by HUVECs in the presence of TCM from SK-MES-1. Data are presented as mean + SEM, three individual experiments were undertaken in triplicate, Kruskal-Wallis-test was used to assess significance with * p

    Article Snippet: Enzyme linked immunosorbent assay (ELISA) The human vascular endothelial growth factor A (VEGF-A) levels in the TCM of A549 cells transfected with negative and miR-140-3p mimics were quantified by using 96-well plates pre-coated with VEGF-A primary antibody by using a specific kit purchased from Aviva System Biology, San Diego, USA.

    Techniques: Cell Culture, Imaging

    Distinctive gene regulation profiles and cell signalling pathways induced by two strands of miR-140 (A) Heatmap of the RNA-seq data showing the altered upregulated (red) and downregulated (green) genes in A549 treated with miR-140-3p and miR-140-5p, respectively. Global transcriptome analysis of 20816 gene targets was performed using the Ion AmpliSeq™ human gene expression panel. (B) The Venn diagram showing the differential gene expression induced by miR-140-3p and miR-140-5p, respectively, based on the RNA-Seq data. (C) Heatmap of the Kinex antibody array data showing the distinctive cell signalling pathways altered by the two strands of miR-140. The values of Z-score reflected positive or negative shifts in differential protein expression fold-changes after normalization by protein concentration and negative miRNA mimics. (D) Evaluation the expression of some key downstream effectors of miR-140-3p in A549 cells after treatments for 48 hours by Western blotting.

    Journal: Oncotarget

    Article Title: Distinct mechanisms by which two forms of miR-140 suppress the malignant properties of lung cancer cells

    doi: 10.18632/oncotarget.26356

    Figure Lengend Snippet: Distinctive gene regulation profiles and cell signalling pathways induced by two strands of miR-140 (A) Heatmap of the RNA-seq data showing the altered upregulated (red) and downregulated (green) genes in A549 treated with miR-140-3p and miR-140-5p, respectively. Global transcriptome analysis of 20816 gene targets was performed using the Ion AmpliSeq™ human gene expression panel. (B) The Venn diagram showing the differential gene expression induced by miR-140-3p and miR-140-5p, respectively, based on the RNA-Seq data. (C) Heatmap of the Kinex antibody array data showing the distinctive cell signalling pathways altered by the two strands of miR-140. The values of Z-score reflected positive or negative shifts in differential protein expression fold-changes after normalization by protein concentration and negative miRNA mimics. (D) Evaluation the expression of some key downstream effectors of miR-140-3p in A549 cells after treatments for 48 hours by Western blotting.

    Article Snippet: Enzyme linked immunosorbent assay (ELISA) The human vascular endothelial growth factor A (VEGF-A) levels in the TCM of A549 cells transfected with negative and miR-140-3p mimics were quantified by using 96-well plates pre-coated with VEGF-A primary antibody by using a specific kit purchased from Aviva System Biology, San Diego, USA.

    Techniques: RNA Sequencing Assay, Expressing, Ab Array, Protein Concentration, Western Blot