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3-D Matrix a549 cells
Click-iT Plus EdU proliferation assay. Plot depicts percent of <t>A549</t> cell nuclei positive for EdU on day 4 in monoculture and the A549/CCL-210 co-cultures, separated by gel and media type. The nondegradable gels contained a peptide crosslinker insensitive to MMP cleavage. GM6001 was added at 10 μM, and the DMSO control media contained 0.05% DMSO. The degradable bars refer to the original experiment in MMP-degradable gels with regular growth media. Results are presented as means ± SEM of three biological replicates of each condition. *p
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Images

1) Product Images from "Epithelial-mesenchymal crosstalk influences cellular behavior in a 3D alveolus-fibroblast model system"

Article Title: Epithelial-mesenchymal crosstalk influences cellular behavior in a 3D alveolus-fibroblast model system

Journal: Biomaterials

doi: 10.1016/j.biomaterials.2017.11.008

Click-iT Plus EdU proliferation assay. Plot depicts percent of A549 cell nuclei positive for EdU on day 4 in monoculture and the A549/CCL-210 co-cultures, separated by gel and media type. The nondegradable gels contained a peptide crosslinker insensitive to MMP cleavage. GM6001 was added at 10 μM, and the DMSO control media contained 0.05% DMSO. The degradable bars refer to the original experiment in MMP-degradable gels with regular growth media. Results are presented as means ± SEM of three biological replicates of each condition. *p
Figure Legend Snippet: Click-iT Plus EdU proliferation assay. Plot depicts percent of A549 cell nuclei positive for EdU on day 4 in monoculture and the A549/CCL-210 co-cultures, separated by gel and media type. The nondegradable gels contained a peptide crosslinker insensitive to MMP cleavage. GM6001 was added at 10 μM, and the DMSO control media contained 0.05% DMSO. The degradable bars refer to the original experiment in MMP-degradable gels with regular growth media. Results are presented as means ± SEM of three biological replicates of each condition. *p

Techniques Used: Proliferation Assay

2) Product Images from "Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device"

Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device

Journal: PLoS ONE

doi: 10.1371/journal.pone.0056448

Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.
Figure Legend Snippet: Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.

Techniques Used:

Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p
Figure Legend Snippet: Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p

Techniques Used: Tube Formation Assay, Cell Culture, Staining

Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.
Figure Legend Snippet: Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.

Techniques Used: Cell Culture

Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.
Figure Legend Snippet: Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.

Techniques Used: Cell Culture, Staining

3) Product Images from "Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device"

Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device

Journal: PLoS ONE

doi: 10.1371/journal.pone.0056448

Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.
Figure Legend Snippet: Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.

Techniques Used:

Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p
Figure Legend Snippet: Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p

Techniques Used: Tube Formation Assay, Cell Culture, Staining

Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.
Figure Legend Snippet: Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.

Techniques Used: Cell Culture

Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.
Figure Legend Snippet: Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.

Techniques Used: Cell Culture, Staining

4) Product Images from "Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device"

Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device

Journal: PLoS ONE

doi: 10.1371/journal.pone.0056448

Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.
Figure Legend Snippet: Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.

Techniques Used:

Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p
Figure Legend Snippet: Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p

Techniques Used: Tube Formation Assay, Cell Culture, Staining

Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.
Figure Legend Snippet: Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.

Techniques Used: Cell Culture

Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.
Figure Legend Snippet: Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.

Techniques Used: Cell Culture, Staining

5) Product Images from "Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device"

Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device

Journal: PLoS ONE

doi: 10.1371/journal.pone.0056448

Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.
Figure Legend Snippet: Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.

Techniques Used:

Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p
Figure Legend Snippet: Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p

Techniques Used: Tube Formation Assay, Cell Culture, Staining

Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.
Figure Legend Snippet: Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.

Techniques Used: Cell Culture

Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.
Figure Legend Snippet: Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.

Techniques Used: Cell Culture, Staining

6) Product Images from "Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device"

Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device

Journal: PLoS ONE

doi: 10.1371/journal.pone.0056448

Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.
Figure Legend Snippet: Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.

Techniques Used:

Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p
Figure Legend Snippet: Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p

Techniques Used: Tube Formation Assay, Cell Culture, Staining

Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.
Figure Legend Snippet: Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.

Techniques Used: Cell Culture

Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.
Figure Legend Snippet: Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.

Techniques Used: Cell Culture, Staining

7) Product Images from "Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device"

Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device

Journal: PLoS ONE

doi: 10.1371/journal.pone.0056448

Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.
Figure Legend Snippet: Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.

Techniques Used:

Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p
Figure Legend Snippet: Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p

Techniques Used: Tube Formation Assay, Cell Culture, Staining

Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.
Figure Legend Snippet: Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.

Techniques Used: Cell Culture

Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.
Figure Legend Snippet: Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.

Techniques Used: Cell Culture, Staining

8) Product Images from "Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device"

Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device

Journal: PLoS ONE

doi: 10.1371/journal.pone.0056448

Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.
Figure Legend Snippet: Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.

Techniques Used:

Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p
Figure Legend Snippet: Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p

Techniques Used: Tube Formation Assay, Cell Culture, Staining

Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.
Figure Legend Snippet: Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.

Techniques Used: Cell Culture

Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.
Figure Legend Snippet: Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.

Techniques Used: Cell Culture, Staining

9) Product Images from "Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device"

Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device

Journal: PLoS ONE

doi: 10.1371/journal.pone.0056448

Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.
Figure Legend Snippet: Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.

Techniques Used:

Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p
Figure Legend Snippet: Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p

Techniques Used: Tube Formation Assay, Cell Culture, Staining

Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.
Figure Legend Snippet: Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.

Techniques Used: Cell Culture

Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.
Figure Legend Snippet: Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.

Techniques Used: Cell Culture, Staining

10) Product Images from "Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device"

Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device

Journal: PLoS ONE

doi: 10.1371/journal.pone.0056448

Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.
Figure Legend Snippet: Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.

Techniques Used:

Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p
Figure Legend Snippet: Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p

Techniques Used: Tube Formation Assay, Cell Culture, Staining

Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.
Figure Legend Snippet: Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.

Techniques Used: Cell Culture

Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.
Figure Legend Snippet: Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.

Techniques Used: Cell Culture, Staining

Related Articles

Fluorescence:

Article Title: Epithelial-mesenchymal crosstalk influences cellular behavior in a 3D alveolus-fibroblast model system
Article Snippet: .. Furthermore, this inhibitor has previously been used with A549 cells to effectively block invasion into a 3D matrix and has been shown to reduce the fluorescence signal from the MMP sensor peptide used in this work [ , , ]. .. To confirm the effectiveness of GM6001 with our co-culture model, we repeated the MMP activity assay with 10 μM GM6001 in the media and observed a reduction by half in the normalized signal on both days compared with the DMSO control group ( ).

Blocking Assay:

Article Title: Epithelial-mesenchymal crosstalk influences cellular behavior in a 3D alveolus-fibroblast model system
Article Snippet: .. Furthermore, this inhibitor has previously been used with A549 cells to effectively block invasion into a 3D matrix and has been shown to reduce the fluorescence signal from the MMP sensor peptide used in this work [ , , ]. .. To confirm the effectiveness of GM6001 with our co-culture model, we repeated the MMP activity assay with 10 μM GM6001 in the media and observed a reduction by half in the normalized signal on both days compared with the DMSO control group ( ).

other:

Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device
Article Snippet: Only 17% of A549 cells formed actin dot-like structures ( ) and the cells displayed an average of 1.3 invadopodia foci per cell with the level near to that of the control group or even lower, suggesting that the EGF-induced formation of invadopodia could be blocked significantly by preincubation of cells with GM6001.

Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device
Article Snippet: The morphological features of A549 cells in 2D and 3D cultures mode were compared.

Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device
Article Snippet: This result indicated that the survival period of A549 cells in gelled BME in our microdevice was long enough for the following studies.

Inhibition:

Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device
Article Snippet: .. GM6001 inhibition assay on invadopodia formation induced by EGF for A549 cells In order to investigate whether the induction of invadopodia formation could be inhibited by MMP inhibitor (GM6001) in A549 cells, we performed the assay on the GM6001/EGF experimental group. .. As shown in , GM6001 neutralized the inductive effect of EGF and led to a decrease in the formation of actin dot-like structures to the level as low as that without EGF stimulation (the control group) or even lower.

Activity Assay:

Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device
Article Snippet: .. We provided evidence that epidermal growth factor receptor (EGFR) signaling played a significant role in invadopodia formation and related ECM degradation activity in A549 cells. ..

Cell Culture:

Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device
Article Snippet: .. Therefore, in our study, we investigated the invasion of lung cancer through the assay of F-actin and cortactin to illustrate invadopodia formation in a 3D microfluidic matrix model. To investigate whether EGF was capable of inducing invadopodia formation for A549 cells cultured in our microfluidic device in 3D matrix, F-actin and cortactin expression were assayed in the three groups of cells. presented a model of invadopodia formation in the device and showed the expression of invadopodia protein of F-actin and cortactin assay by immunofluorescence. .. In the latter figure, F-actin expression was indicated in red color and cortactin expression in green, whereas merged images with yellow color suggested colocalization of the two proteins.

Expressing:

Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device
Article Snippet: .. Therefore, in our study, we investigated the invasion of lung cancer through the assay of F-actin and cortactin to illustrate invadopodia formation in a 3D microfluidic matrix model. To investigate whether EGF was capable of inducing invadopodia formation for A549 cells cultured in our microfluidic device in 3D matrix, F-actin and cortactin expression were assayed in the three groups of cells. presented a model of invadopodia formation in the device and showed the expression of invadopodia protein of F-actin and cortactin assay by immunofluorescence. .. In the latter figure, F-actin expression was indicated in red color and cortactin expression in green, whereas merged images with yellow color suggested colocalization of the two proteins.

Immunofluorescence:

Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device
Article Snippet: .. Therefore, in our study, we investigated the invasion of lung cancer through the assay of F-actin and cortactin to illustrate invadopodia formation in a 3D microfluidic matrix model. To investigate whether EGF was capable of inducing invadopodia formation for A549 cells cultured in our microfluidic device in 3D matrix, F-actin and cortactin expression were assayed in the three groups of cells. presented a model of invadopodia formation in the device and showed the expression of invadopodia protein of F-actin and cortactin assay by immunofluorescence. .. In the latter figure, F-actin expression was indicated in red color and cortactin expression in green, whereas merged images with yellow color suggested colocalization of the two proteins.

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    3-D Matrix a549 cells
    Click-iT Plus EdU proliferation assay. Plot depicts percent of <t>A549</t> cell nuclei positive for EdU on day 4 in monoculture and the A549/CCL-210 co-cultures, separated by gel and media type. The nondegradable gels contained a peptide crosslinker insensitive to MMP cleavage. GM6001 was added at 10 μM, and the DMSO control media contained 0.05% DMSO. The degradable bars refer to the original experiment in MMP-degradable gels with regular growth media. Results are presented as means ± SEM of three biological replicates of each condition. *p
    A549 Cells, supplied by 3-D Matrix, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    3-D Matrix human non small cell lung cancer a549 cells
    The percentages of apoptotic <t>A549</t> cells. A549 cells were cultured in maintenance medium for 24h and continually cultured in triplicate in the same medium or mixture of maintenance medium and CAF matrix in the presence or absence of the PI3K or GRP78 inhibitor on the 3D chambers for 24h. Subsequently, the cells were stained with Hochest33342 and PI, imaged and the percentages of apoptotic A549 cells were counted. Data are representative images (Fig 4A) (magnification x 200) or expressed as the means ± SD of the percentages of apoptotic cells in individual groups of cells from three separate experiments (Fig 4B). * P
    Human Non Small Cell Lung Cancer A549 Cells, supplied by 3-D Matrix, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human non small cell lung cancer a549 cells/product/3-D Matrix
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
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    Click-iT Plus EdU proliferation assay. Plot depicts percent of A549 cell nuclei positive for EdU on day 4 in monoculture and the A549/CCL-210 co-cultures, separated by gel and media type. The nondegradable gels contained a peptide crosslinker insensitive to MMP cleavage. GM6001 was added at 10 μM, and the DMSO control media contained 0.05% DMSO. The degradable bars refer to the original experiment in MMP-degradable gels with regular growth media. Results are presented as means ± SEM of three biological replicates of each condition. *p

    Journal: Biomaterials

    Article Title: Epithelial-mesenchymal crosstalk influences cellular behavior in a 3D alveolus-fibroblast model system

    doi: 10.1016/j.biomaterials.2017.11.008

    Figure Lengend Snippet: Click-iT Plus EdU proliferation assay. Plot depicts percent of A549 cell nuclei positive for EdU on day 4 in monoculture and the A549/CCL-210 co-cultures, separated by gel and media type. The nondegradable gels contained a peptide crosslinker insensitive to MMP cleavage. GM6001 was added at 10 μM, and the DMSO control media contained 0.05% DMSO. The degradable bars refer to the original experiment in MMP-degradable gels with regular growth media. Results are presented as means ± SEM of three biological replicates of each condition. *p

    Article Snippet: Furthermore, this inhibitor has previously been used with A549 cells to effectively block invasion into a 3D matrix and has been shown to reduce the fluorescence signal from the MMP sensor peptide used in this work [ , , ].

    Techniques: Proliferation Assay

    Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.

    Journal: PLoS ONE

    Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device

    doi: 10.1371/journal.pone.0056448

    Figure Lengend Snippet: Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.

    Article Snippet: GM6001 inhibition assay on invadopodia formation induced by EGF for A549 cells In order to investigate whether the induction of invadopodia formation could be inhibited by MMP inhibitor (GM6001) in A549 cells, we performed the assay on the GM6001/EGF experimental group.

    Techniques:

    Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p

    Journal: PLoS ONE

    Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device

    doi: 10.1371/journal.pone.0056448

    Figure Lengend Snippet: Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p

    Article Snippet: GM6001 inhibition assay on invadopodia formation induced by EGF for A549 cells In order to investigate whether the induction of invadopodia formation could be inhibited by MMP inhibitor (GM6001) in A549 cells, we performed the assay on the GM6001/EGF experimental group.

    Techniques: Tube Formation Assay, Cell Culture, Staining

    Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.

    Journal: PLoS ONE

    Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device

    doi: 10.1371/journal.pone.0056448

    Figure Lengend Snippet: Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.

    Article Snippet: GM6001 inhibition assay on invadopodia formation induced by EGF for A549 cells In order to investigate whether the induction of invadopodia formation could be inhibited by MMP inhibitor (GM6001) in A549 cells, we performed the assay on the GM6001/EGF experimental group.

    Techniques: Cell Culture

    Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.

    Journal: PLoS ONE

    Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device

    doi: 10.1371/journal.pone.0056448

    Figure Lengend Snippet: Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.

    Article Snippet: GM6001 inhibition assay on invadopodia formation induced by EGF for A549 cells In order to investigate whether the induction of invadopodia formation could be inhibited by MMP inhibitor (GM6001) in A549 cells, we performed the assay on the GM6001/EGF experimental group.

    Techniques: Cell Culture, Staining

    The percentages of apoptotic A549 cells. A549 cells were cultured in maintenance medium for 24h and continually cultured in triplicate in the same medium or mixture of maintenance medium and CAF matrix in the presence or absence of the PI3K or GRP78 inhibitor on the 3D chambers for 24h. Subsequently, the cells were stained with Hochest33342 and PI, imaged and the percentages of apoptotic A549 cells were counted. Data are representative images (Fig 4A) (magnification x 200) or expressed as the means ± SD of the percentages of apoptotic cells in individual groups of cells from three separate experiments (Fig 4B). * P

    Journal: PLoS ONE

    Article Title: Cancer Associated Fibroblast-Derived Hepatocyte Growth Factor Inhibits the Paclitaxel-Induced Apoptosis of Lung Cancer A549 Cells by Up-Regulating the PI3K/Akt and GRP78 Signaling on a Microfluidic Platform

    doi: 10.1371/journal.pone.0129593

    Figure Lengend Snippet: The percentages of apoptotic A549 cells. A549 cells were cultured in maintenance medium for 24h and continually cultured in triplicate in the same medium or mixture of maintenance medium and CAF matrix in the presence or absence of the PI3K or GRP78 inhibitor on the 3D chambers for 24h. Subsequently, the cells were stained with Hochest33342 and PI, imaged and the percentages of apoptotic A549 cells were counted. Data are representative images (Fig 4A) (magnification x 200) or expressed as the means ± SD of the percentages of apoptotic cells in individual groups of cells from three separate experiments (Fig 4B). * P

    Article Snippet: In this study, we examined the impact of CAF or HGF on the Met/PI3K/AKT phosphorylation, GRP78 expression and paclitaxel-induced apoptosis in human non-small cell lung cancer A549 cells cultured in the 3D matrix.

    Techniques: Cell Culture, Staining

    The design and validation of a 3D culture microfluidic chip. (a)The schematic design of the microfluidic chip with CGG and downstream cell chambers (the upper panel) and the fabricated chip with pumping machine (the lower panel). (b)The diffused Rh-123 in the 3D chamber within 30 min and > 95% cells were viable (green). Magnification ×100. (c) The morphological features of A549 cells in the 3D chamber without or with CAF matrix. The white arrows indicate apoptotic cells. (d)The α-SMA immunofluorescence assay of HFL1 cells. HFL1 cells induced by A549 medium showed a positive α-SMA staining (right) compared to the untreated HFL1 (left). Magnification ×400. (e) Immunohistochemistry assay for lung cancer tissues. The expression of α-SMA protein in the lung cancer tissues is higher than that in adjacent tissues. Magnification ×200.

    Journal: PLoS ONE

    Article Title: Cancer Associated Fibroblast-Derived Hepatocyte Growth Factor Inhibits the Paclitaxel-Induced Apoptosis of Lung Cancer A549 Cells by Up-Regulating the PI3K/Akt and GRP78 Signaling on a Microfluidic Platform

    doi: 10.1371/journal.pone.0129593

    Figure Lengend Snippet: The design and validation of a 3D culture microfluidic chip. (a)The schematic design of the microfluidic chip with CGG and downstream cell chambers (the upper panel) and the fabricated chip with pumping machine (the lower panel). (b)The diffused Rh-123 in the 3D chamber within 30 min and > 95% cells were viable (green). Magnification ×100. (c) The morphological features of A549 cells in the 3D chamber without or with CAF matrix. The white arrows indicate apoptotic cells. (d)The α-SMA immunofluorescence assay of HFL1 cells. HFL1 cells induced by A549 medium showed a positive α-SMA staining (right) compared to the untreated HFL1 (left). Magnification ×400. (e) Immunohistochemistry assay for lung cancer tissues. The expression of α-SMA protein in the lung cancer tissues is higher than that in adjacent tissues. Magnification ×200.

    Article Snippet: In this study, we examined the impact of CAF or HGF on the Met/PI3K/AKT phosphorylation, GRP78 expression and paclitaxel-induced apoptosis in human non-small cell lung cancer A549 cells cultured in the 3D matrix.

    Techniques: Chromatin Immunoprecipitation, Immunofluorescence, Staining, Immunohistochemistry, Expressing

    Western blot analysis of the c-Met/PI3K/AKT activation and GRP78 expression in A549 cells. A549 cells were cultured in the condition as described above and the relative levels of phosphorylated Met, PI3Kp85, AKT and GRP78 expression in the different groups of cells were characterized by Western blot assays and quantified. Data are representative images and expressed as the means ± SD of each protein in individual groups of cells from three separate experiments. * P

    Journal: PLoS ONE

    Article Title: Cancer Associated Fibroblast-Derived Hepatocyte Growth Factor Inhibits the Paclitaxel-Induced Apoptosis of Lung Cancer A549 Cells by Up-Regulating the PI3K/Akt and GRP78 Signaling on a Microfluidic Platform

    doi: 10.1371/journal.pone.0129593

    Figure Lengend Snippet: Western blot analysis of the c-Met/PI3K/AKT activation and GRP78 expression in A549 cells. A549 cells were cultured in the condition as described above and the relative levels of phosphorylated Met, PI3Kp85, AKT and GRP78 expression in the different groups of cells were characterized by Western blot assays and quantified. Data are representative images and expressed as the means ± SD of each protein in individual groups of cells from three separate experiments. * P

    Article Snippet: In this study, we examined the impact of CAF or HGF on the Met/PI3K/AKT phosphorylation, GRP78 expression and paclitaxel-induced apoptosis in human non-small cell lung cancer A549 cells cultured in the 3D matrix.

    Techniques: Western Blot, Activation Assay, Expressing, Cell Culture

    Immunofluorescent analysis of the MET/PI3K/AKT activation and GRP78 expression on the microfluidic chip. A549 cells were cultured in triplicate in the maintenance medium alone, mixed with the CAF matrix in the presence or absence of anti-HGF or containing 40 ng/ml of human HGF in the 3D chambers for 48h. The cells were stained with the indicated FITC-conjugated antibodies, and examined under a fluorescent microscope. Furthermore, the cells were cultured in the mixture of maintenance medium and CAF matrix in the presence or absence of an inhibitor for c-Met, PI3K or GRP78 for 48h and stained as described above. Data are representative images (magnification x 200) from three separate experiments. (A)The CAF matrix or HGF enhances the c-Met/PI3K/AKT activation and GRP78 expression in A549 cells. (B)The effect of an inhibitor of c-Met, PI3K or GRP78 on the CAF-enhanced c-Met/PI3K/AKT activation and GRP78 expression in A549 cells.

    Journal: PLoS ONE

    Article Title: Cancer Associated Fibroblast-Derived Hepatocyte Growth Factor Inhibits the Paclitaxel-Induced Apoptosis of Lung Cancer A549 Cells by Up-Regulating the PI3K/Akt and GRP78 Signaling on a Microfluidic Platform

    doi: 10.1371/journal.pone.0129593

    Figure Lengend Snippet: Immunofluorescent analysis of the MET/PI3K/AKT activation and GRP78 expression on the microfluidic chip. A549 cells were cultured in triplicate in the maintenance medium alone, mixed with the CAF matrix in the presence or absence of anti-HGF or containing 40 ng/ml of human HGF in the 3D chambers for 48h. The cells were stained with the indicated FITC-conjugated antibodies, and examined under a fluorescent microscope. Furthermore, the cells were cultured in the mixture of maintenance medium and CAF matrix in the presence or absence of an inhibitor for c-Met, PI3K or GRP78 for 48h and stained as described above. Data are representative images (magnification x 200) from three separate experiments. (A)The CAF matrix or HGF enhances the c-Met/PI3K/AKT activation and GRP78 expression in A549 cells. (B)The effect of an inhibitor of c-Met, PI3K or GRP78 on the CAF-enhanced c-Met/PI3K/AKT activation and GRP78 expression in A549 cells.

    Article Snippet: In this study, we examined the impact of CAF or HGF on the Met/PI3K/AKT phosphorylation, GRP78 expression and paclitaxel-induced apoptosis in human non-small cell lung cancer A549 cells cultured in the 3D matrix.

    Techniques: Activation Assay, Expressing, Chromatin Immunoprecipitation, Cell Culture, Staining, Microscopy

    Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.

    Journal: PLoS ONE

    Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device

    doi: 10.1371/journal.pone.0056448

    Figure Lengend Snippet: Actual invadopodia formation of A549 cells in control group (A), EGF group (B), and GM6001/EGF group (C) in 3D extracellular matrix in the microfluidic device with confocal system. Invadopodia could be obviously induced by EGF in (B), while this induction could be inhibited by GM6001 in (C). White arrowheads represented invadopodia. Magnification: ×1200.

    Article Snippet: In this work, we built a straightforward and practical microfluidic 3D culture device to assay the formation of invadopodia by human non-small cell lung cancer cell line A549.

    Techniques:

    Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p

    Journal: PLoS ONE

    Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device

    doi: 10.1371/journal.pone.0056448

    Figure Lengend Snippet: Invadopodia formation assay and quantification analysis with confocal system in A549 cells. The cells in control group (A), EGF group (B), and GM6001/EGF group (C), were cultured on a 3D microfluidic device for 12 h. The cells were stained green represented combined with cortactin, stained red represented combined with F-actin, and arrowheads in merge pictures indicated cells displaying invadopodia. (D) The percent of the cells with invadopodia formation. (E) The average number of invadopodia per cell. Error bars represented the SD of three different determinations. *Statistically significant between control group and EGF group; **statistically significant between EGF group and GM6001/EGF group, p

    Article Snippet: In this work, we built a straightforward and practical microfluidic 3D culture device to assay the formation of invadopodia by human non-small cell lung cancer cell line A549.

    Techniques: Tube Formation Assay, Cell Culture, Staining

    Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.

    Journal: PLoS ONE

    Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device

    doi: 10.1371/journal.pone.0056448

    Figure Lengend Snippet: Morphology of A549 cells on 2D surfaces and in 3D matrix. (A) A549 cells were cultured on 2D surfaces. Cells exhibited to be flat with several protrusions. (B) A549 cells were cultured in 3D matrix. A majority of the cells were round without protrusions. Magnification: ×200.

    Article Snippet: In this work, we built a straightforward and practical microfluidic 3D culture device to assay the formation of invadopodia by human non-small cell lung cancer cell line A549.

    Techniques: Cell Culture

    Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.

    Journal: PLoS ONE

    Article Title: Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device

    doi: 10.1371/journal.pone.0056448

    Figure Lengend Snippet: Fluoresent analysis of apoptotic in A549 cells. Cells were cultured on the 3D microfluidic device for 96 h and then stained by Hoechst and PI. Live cells were stained blue and dead cells were stained red. Magnification: ×200.

    Article Snippet: In this work, we built a straightforward and practical microfluidic 3D culture device to assay the formation of invadopodia by human non-small cell lung cancer cell line A549.

    Techniques: Cell Culture, Staining