a23187  (Alomone Labs)

Journal: International Journal of Nanomedicine

Article Title: A TEM-traceable physiologically functional gold nanoprobe that permeates non-endocytic cells

doi: 10.2147/IJN.S168149

Figure Lengend Snippet: Membrane-permeable CPP-MT and CPP-MT-AuNP inhibit acrosomal exocytosis in sperm. Notes: Human sperm were incubated for 1 hour at 37°C in HTF medium with increasing concentrations of recombinant CPP-MT unconjugated ( A ) or conjugated to AuNPs ( B ). Afterward, the cells were incubated for 15 minutes without stimulus (control) or with 10 µM A23187. After incubation, the samples were fixed and the acrosomal exocytosis index was evaluated using PSL-FITC and calculated as detailed in section “Materials and methods”. The data represent mean values ± SEM and were calculated from three independent experiments. Two-way ANOVA shows statistically significant difference (*** P

Article Snippet: A23187 was from Alomone Labs.

Techniques: Conditioned Place Preference, Incubation, Recombinant

Journal: PLoS ONE

Article Title: MARCKS Protein Is Phosphorylated and Regulates Calcium Mobilization during Human Acrosomal Exocytosis

doi: 10.1371/journal.pone.0064551

Figure Lengend Snippet: Assessing MARCKS function on acrosomal exocytosis in non-permeabilized sperm. ( A ) Capacitated and non-permeabilized sperm were treated for 30 minutes at 37°C with increasing concentrations of a permeable tetramethylrhodamine-labeled MARCKS ED peptide (ED-TMR). Acrosomal exocytosis was then initiated by adding 10 µM calcium ionophore A23187 (circles), 200 nM PMA (squares) or 15 µM progesterone (triangles), and the incubation continued for 15 minutes. The percentage of reacted sperm was normalized as described in Materials and Methods . The data represent the means±SEM of at least three independent experiments. The asterisks indicate significant differences from similar conditions without ED-TMR (*, p

Article Snippet: When data were normalized against MARCKS, similar results were obtained (see for the effect of A23187; the results for PMA and progesterone are not shown).

Techniques: Labeling, Incubation

Journal: Experimental Animals

Article Title: Novel phenotype in beagle dogs characterized by skin response to compound 48/80 focusing on skin mast cell degranulation

doi: 10.1538/expanim.15-0004

Figure Lengend Snippet: Illustration of main pathways to skin response via mast cells based on results shown in Fig. 4 . Nonresponder dogs showed skin responses to HCO-60, histamine dihydrochloride, concanavalin A, and A23187 comparable to those of wild-type dogs but no response to compound 48/80; the dysfunctional route in NR dogs would be downstream of the GPCR.

Article Snippet: ChemicalsCompound 48/80 was purchased from Sigma-Aldrich Corporation (St. Louis, MO, USA), HCO-60 was purchased from Nihon Surfactant Kogyo K.K. (Tokyo, Japan), histamine dihydrochloride was purchased from Nacalai Tesque, Inc. (Kyoto, Japan), Concanavalin A was purchased from Seikagaku Corp. (Tokyo, Japan), and A23187 was purchased from Alomone Labs, Ltd.. (Jerusalem, Israel).

Techniques:

Journal: Experimental Animals

Article Title: Novel phenotype in beagle dogs characterized by skin response to compound 48/80 focusing on skin mast cell degranulation

doi: 10.1538/expanim.15-0004

Figure Lengend Snippet: Vascular permeability to various compounds. Compound 48/80 (A), HCO-60 (B), histamine dihydrochloride (C), concanavalin A (D), and A23187 (E) at 0.01–100 mg/ml ID injected at 0.05 ml/site into the shaved thorax of anesthetized wild-type (WT) and nonresponder (NR) dogs; Evans blue (1 mg/kg) dissolved in saline IV injected just prior to ID injections; and 10 min later, ID injection sites collected for determination of pigment leakage. The significance of the differences between WT and NR groups was determined by Student’s t -test. ** P

Article Snippet: ChemicalsCompound 48/80 was purchased from Sigma-Aldrich Corporation (St. Louis, MO, USA), HCO-60 was purchased from Nihon Surfactant Kogyo K.K. (Tokyo, Japan), histamine dihydrochloride was purchased from Nacalai Tesque, Inc. (Kyoto, Japan), Concanavalin A was purchased from Seikagaku Corp. (Tokyo, Japan), and A23187 was purchased from Alomone Labs, Ltd.. (Jerusalem, Israel).

Techniques: Permeability, Injection