a13 vl  (New England Biolabs)


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    Structured Review

    New England Biolabs a13 vl
    Competition ELISA for comparison of relative affinities of anti-EGFR IgGs. ER414 is a fully human IgG1 originated from the mAb <t>A13</t> by guided panning. Binding inhibition of anti-EGFR IgGs to EGFR was analyzed on EGFR-coated plates with different concentrations
    A13 Vl, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a13 vl/product/New England Biolabs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a13 vl - by Bioz Stars, 2020-08
    85/100 stars

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    a13 vh

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    1) Product Images from "Conversion of a murine monoclonal antibody A13 targeting epidermal growth factor receptor to a human monoclonal antibody by guided selection"

    Article Title: Conversion of a murine monoclonal antibody A13 targeting epidermal growth factor receptor to a human monoclonal antibody by guided selection

    Journal: Experimental & Molecular Medicine

    doi: 10.3858/emm.2012.44.1.005

    Competition ELISA for comparison of relative affinities of anti-EGFR IgGs. ER414 is a fully human IgG1 originated from the mAb A13 by guided panning. Binding inhibition of anti-EGFR IgGs to EGFR was analyzed on EGFR-coated plates with different concentrations
    Figure Legend Snippet: Competition ELISA for comparison of relative affinities of anti-EGFR IgGs. ER414 is a fully human IgG1 originated from the mAb A13 by guided panning. Binding inhibition of anti-EGFR IgGs to EGFR was analyzed on EGFR-coated plates with different concentrations

    Techniques Used: Enzyme-linked Immunosorbent Assay, Binding Assay, Inhibition

    Competition ELISA to analyze whether the ER414 has the same binding site with the murine counterpart A13 mAb. The competition was performed on the EGFR-coated plates with 1 µg of biotinylated ER414 and different concentrations of free ER414, A13
    Figure Legend Snippet: Competition ELISA to analyze whether the ER414 has the same binding site with the murine counterpart A13 mAb. The competition was performed on the EGFR-coated plates with 1 µg of biotinylated ER414 and different concentrations of free ER414, A13

    Techniques Used: Enzyme-linked Immunosorbent Assay, Binding Assay

    Competition ELISA for measuring relative affinities of anti-EGFR human scFvs which were isolated by guided panning of mAb A13. The inhibition of binding of anti-EGFR human scFvs to EGFR was analyzed on EGFR-coated plates with different concentrations
    Figure Legend Snippet: Competition ELISA for measuring relative affinities of anti-EGFR human scFvs which were isolated by guided panning of mAb A13. The inhibition of binding of anti-EGFR human scFvs to EGFR was analyzed on EGFR-coated plates with different concentrations

    Techniques Used: Enzyme-linked Immunosorbent Assay, Isolation, Inhibition, Binding Assay

    FACS analysis for binding activity of anti-EGFR human scFvs to cell surface-expressed EGFR, in which the scFvs were isolated by guided panning of mAb A13. Differential binding of scFvs to EGFR-positive A431 tumor cells and EGFR-negative HL60 cells (indicated
    Figure Legend Snippet: FACS analysis for binding activity of anti-EGFR human scFvs to cell surface-expressed EGFR, in which the scFvs were isolated by guided panning of mAb A13. Differential binding of scFvs to EGFR-positive A431 tumor cells and EGFR-negative HL60 cells (indicated

    Techniques Used: FACS, Binding Assay, Activity Assay, Isolation

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Conversion of a murine monoclonal antibody A13 targeting epidermal growth factor receptor to a human monoclonal antibody by guided selection
    Article Snippet: .. Again the PCR DNAs of A13 VH and A13 VL were amplified with primers H-extension(F)/H-extension(R) and K-extension(F)/K-extension(R) ( ) and inserted to pSC73His vector ( ) by digestion with Sfi I/BstE II (New England Bio Labs, Beverly, MA) for VH and BstE II/Not I (New England Bio Lab) for VL , which was designated as pSC73His-13VH -13VL . .. The pSC73His-13VH -13VL plasmid was transformed to electrocompetant E. coli XL1-Blue (Stratagene, La Jolla, CA) by electroporation, and plated on the SB (Super Broth; ) agar plates containing 50 µg/ml of carbenicillin.

    Amplification:

    Article Title: Conversion of a murine monoclonal antibody A13 targeting epidermal growth factor receptor to a human monoclonal antibody by guided selection
    Article Snippet: .. Again the PCR DNAs of A13 VH and A13 VL were amplified with primers H-extension(F)/H-extension(R) and K-extension(F)/K-extension(R) ( ) and inserted to pSC73His vector ( ) by digestion with Sfi I/BstE II (New England Bio Labs, Beverly, MA) for VH and BstE II/Not I (New England Bio Lab) for VL , which was designated as pSC73His-13VH -13VL . .. The pSC73His-13VH -13VL plasmid was transformed to electrocompetant E. coli XL1-Blue (Stratagene, La Jolla, CA) by electroporation, and plated on the SB (Super Broth; ) agar plates containing 50 µg/ml of carbenicillin.

    Plasmid Preparation:

    Article Title: Conversion of a murine monoclonal antibody A13 targeting epidermal growth factor receptor to a human monoclonal antibody by guided selection
    Article Snippet: .. Again the PCR DNAs of A13 VH and A13 VL were amplified with primers H-extension(F)/H-extension(R) and K-extension(F)/K-extension(R) ( ) and inserted to pSC73His vector ( ) by digestion with Sfi I/BstE II (New England Bio Labs, Beverly, MA) for VH and BstE II/Not I (New England Bio Lab) for VL , which was designated as pSC73His-13VH -13VL . .. The pSC73His-13VH -13VL plasmid was transformed to electrocompetant E. coli XL1-Blue (Stratagene, La Jolla, CA) by electroporation, and plated on the SB (Super Broth; ) agar plates containing 50 µg/ml of carbenicillin.

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    New England Biolabs a13 vl
    Competition ELISA for comparison of relative affinities of anti-EGFR IgGs. ER414 is a fully human IgG1 originated from the mAb <t>A13</t> by guided panning. Binding inhibition of anti-EGFR IgGs to EGFR was analyzed on EGFR-coated plates with different concentrations
    A13 Vl, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a13 vl/product/New England Biolabs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a13 vl - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

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    Competition ELISA for comparison of relative affinities of anti-EGFR IgGs. ER414 is a fully human IgG1 originated from the mAb A13 by guided panning. Binding inhibition of anti-EGFR IgGs to EGFR was analyzed on EGFR-coated plates with different concentrations

    Journal: Experimental & Molecular Medicine

    Article Title: Conversion of a murine monoclonal antibody A13 targeting epidermal growth factor receptor to a human monoclonal antibody by guided selection

    doi: 10.3858/emm.2012.44.1.005

    Figure Lengend Snippet: Competition ELISA for comparison of relative affinities of anti-EGFR IgGs. ER414 is a fully human IgG1 originated from the mAb A13 by guided panning. Binding inhibition of anti-EGFR IgGs to EGFR was analyzed on EGFR-coated plates with different concentrations

    Article Snippet: Again the PCR DNAs of A13 VH and A13 VL were amplified with primers H-extension(F)/H-extension(R) and K-extension(F)/K-extension(R) ( ) and inserted to pSC73His vector ( ) by digestion with Sfi I/BstE II (New England Bio Labs, Beverly, MA) for VH and BstE II/Not I (New England Bio Lab) for VL , which was designated as pSC73His-13VH -13VL .

    Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay, Inhibition

    Competition ELISA to analyze whether the ER414 has the same binding site with the murine counterpart A13 mAb. The competition was performed on the EGFR-coated plates with 1 µg of biotinylated ER414 and different concentrations of free ER414, A13

    Journal: Experimental & Molecular Medicine

    Article Title: Conversion of a murine monoclonal antibody A13 targeting epidermal growth factor receptor to a human monoclonal antibody by guided selection

    doi: 10.3858/emm.2012.44.1.005

    Figure Lengend Snippet: Competition ELISA to analyze whether the ER414 has the same binding site with the murine counterpart A13 mAb. The competition was performed on the EGFR-coated plates with 1 µg of biotinylated ER414 and different concentrations of free ER414, A13

    Article Snippet: Again the PCR DNAs of A13 VH and A13 VL were amplified with primers H-extension(F)/H-extension(R) and K-extension(F)/K-extension(R) ( ) and inserted to pSC73His vector ( ) by digestion with Sfi I/BstE II (New England Bio Labs, Beverly, MA) for VH and BstE II/Not I (New England Bio Lab) for VL , which was designated as pSC73His-13VH -13VL .

    Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay

    Competition ELISA for measuring relative affinities of anti-EGFR human scFvs which were isolated by guided panning of mAb A13. The inhibition of binding of anti-EGFR human scFvs to EGFR was analyzed on EGFR-coated plates with different concentrations

    Journal: Experimental & Molecular Medicine

    Article Title: Conversion of a murine monoclonal antibody A13 targeting epidermal growth factor receptor to a human monoclonal antibody by guided selection

    doi: 10.3858/emm.2012.44.1.005

    Figure Lengend Snippet: Competition ELISA for measuring relative affinities of anti-EGFR human scFvs which were isolated by guided panning of mAb A13. The inhibition of binding of anti-EGFR human scFvs to EGFR was analyzed on EGFR-coated plates with different concentrations

    Article Snippet: Again the PCR DNAs of A13 VH and A13 VL were amplified with primers H-extension(F)/H-extension(R) and K-extension(F)/K-extension(R) ( ) and inserted to pSC73His vector ( ) by digestion with Sfi I/BstE II (New England Bio Labs, Beverly, MA) for VH and BstE II/Not I (New England Bio Lab) for VL , which was designated as pSC73His-13VH -13VL .

    Techniques: Enzyme-linked Immunosorbent Assay, Isolation, Inhibition, Binding Assay

    FACS analysis for binding activity of anti-EGFR human scFvs to cell surface-expressed EGFR, in which the scFvs were isolated by guided panning of mAb A13. Differential binding of scFvs to EGFR-positive A431 tumor cells and EGFR-negative HL60 cells (indicated

    Journal: Experimental & Molecular Medicine

    Article Title: Conversion of a murine monoclonal antibody A13 targeting epidermal growth factor receptor to a human monoclonal antibody by guided selection

    doi: 10.3858/emm.2012.44.1.005

    Figure Lengend Snippet: FACS analysis for binding activity of anti-EGFR human scFvs to cell surface-expressed EGFR, in which the scFvs were isolated by guided panning of mAb A13. Differential binding of scFvs to EGFR-positive A431 tumor cells and EGFR-negative HL60 cells (indicated

    Article Snippet: Again the PCR DNAs of A13 VH and A13 VL were amplified with primers H-extension(F)/H-extension(R) and K-extension(F)/K-extension(R) ( ) and inserted to pSC73His vector ( ) by digestion with Sfi I/BstE II (New England Bio Labs, Beverly, MA) for VH and BstE II/Not I (New England Bio Lab) for VL , which was designated as pSC73His-13VH -13VL .

    Techniques: FACS, Binding Assay, Activity Assay, Isolation