Journal: bioRxiv
Article Title: Sex-specific glycosylation of secreted immunomodulatory proteins in the filarial nematode Brugia malayi
doi: 10.1101/2021.02.24.432741
Figure Lengend Snippet: Fluorescence microscopy of male and female B. malayi adult worm heads and tails stained with fluorophore-labeled lectins. A. Heads of adult male and female worms. B. Tails of adult male and female worms. Images show full adult worms fixed in 4% formaldehyde 2:1 in heptane and stained with AAL-FITC for Fucosylated glycan epitopes (Green), GS1-Rhodamine for Galactosylated glycan epitopes (Yellow), Phalloidin for actin (Magenta) and DAPI for DNA (Cyan). Images are taken at 20x magnification for both males and females. Images shown are representative of multiple biological replicates (N=3). Arrow heads point to sensory organs in cephalic regions of both males and females, arrows highlight the intestines of both males and females and the pointy arrow indicates male spicules (reproductive organs).
Article Snippet: Elution was done in 4 bed volumes with corresponding elution buffers (Glycoprotein Eluting Solution, Cat. No. ES-3100 Vector labs for AAL column and 0.1M Melibiose monohydrate for GSI Cat# AK-2401-2).
Techniques: Fluorescence, Microscopy, Staining, Labeling