a pleuropneumoniae field  (ATCC)

Journal:

Article Title: Isolation of an Atypical Strain of Actinobacillus pleuropneumoniae Serotype 1 with a Truncated Lipopolysaccharide Outer Core and No O-Antigen

doi: 10.1128/JCM.43.7.3522-3525.2005

Figure Lengend Snippet: Immunoblot of whole-cell, proteinase K-treated preparations of A. pleuropneumoniae reference strain S4074 (lane 1) and strain FMV 91-6514 (lane 2). The immunoblot was probed with a monoclonal antibody against A. pleuropneumoniae serotype 1 O-antigen. Molecular mass markers (in kilodaltons) are indicated on the left.

Article Snippet: A. pleuropneumoniae field isolate FMV 91-6514 and reference strain S4074 (ATCC 27088) were grown on brain heart infusion agar plates (Difco Laboratories, Detroit, MI) supplemented with 15 μg/ml of NAD.

Techniques: Western Blot

Journal:

Article Title: Isolation of an Atypical Strain of Actinobacillus pleuropneumoniae Serotype 1 with a Truncated Lipopolysaccharide Outer Core and No O-Antigen

doi: 10.1128/JCM.43.7.3522-3525.2005

Figure Lengend Snippet: Silver-stained Tricine-SDS-PAGE profiles of whole-cell, proteinase K-treated preparations of A. pleuropneumoniae reference strain S4074 (lane 2), strain FMV 91-6514 (lane 3), and strain FMV 91-6514 recovered from lungs of experimentally infected animals (lane 4). For comparison, LPS from Salmonella enterica serovar Typhimurium TV119 (Ra mutant, lane 1) and SL1181 (Re mutant, lane 5) are shown. Molecular mass markers (in kilodaltons) are indicated on the left.

Article Snippet: A. pleuropneumoniae field isolate FMV 91-6514 and reference strain S4074 (ATCC 27088) were grown on brain heart infusion agar plates (Difco Laboratories, Detroit, MI) supplemented with 15 μg/ml of NAD.

Techniques: Staining, SDS Page, Infection, Mutagenesis

Journal:

Article Title: Isolation of an Atypical Strain of Actinobacillus pleuropneumoniae Serotype 1 with a Truncated Lipopolysaccharide Outer Core and No O-Antigen

doi: 10.1128/JCM.43.7.3522-3525.2005

Figure Lengend Snippet: Primers used for PCR amplification of A. pleuropneumoniae serotype 1 genes involved in LPS biosynthesis

Article Snippet: A. pleuropneumoniae field isolate FMV 91-6514 and reference strain S4074 (ATCC 27088) were grown on brain heart infusion agar plates (Difco Laboratories, Detroit, MI) supplemented with 15 μg/ml of NAD.

Techniques: Amplification, Sequencing

Journal:

Article Title: Isolation of an Atypical Strain of Actinobacillus pleuropneumoniae Serotype 1 with a Truncated Lipopolysaccharide Outer Core and No O-Antigen

doi: 10.1128/JCM.43.7.3522-3525.2005

Figure Lengend Snippet: Negative ion capillary electrophoresis-electrospray-MS data and proposed compositions of O-deacylated LPS from A. pleuropneumoniae strain FMV 91-6514 a

Article Snippet: A. pleuropneumoniae field isolate FMV 91-6514 and reference strain S4074 (ATCC 27088) were grown on brain heart infusion agar plates (Difco Laboratories, Detroit, MI) supplemented with 15 μg/ml of NAD.

Techniques: Electrophoresis

Journal: BMC Genomics

Article Title: Transcriptional profiling of Actinobacillus pleuropneumoniae during the acute phase of a natural infection in pigs

doi: 10.1186/1471-2164-11-98

Figure Lengend Snippet: Hierarchical clustering of reference and field strains of A. pleuropneumoniae , including strain 896-07, based on CGH data obtained with AppChip2 . Strains are identified according to "serotype-strain number". Field strains are the same as those used by Gouré et al. R: reference strain. NT: Non-typeable.

Article Snippet: The A. pleuropneumoniae field strain 896-07 was isolated directly from the infected lung tissues and was routinely grown in BHI medium (Difco) supplemented with either 15 μg/ml (agar) or 5 μg/ml (broth) of NAD.

Techniques:

Journal: BMC Genomics

Article Title: Transcriptional profiling of Actinobacillus pleuropneumoniae during the acute phase of a natural infection in pigs

doi: 10.1186/1471-2164-11-98

Figure Lengend Snippet: Functional classification of A. pleuropneumoniae genes that are differentially expressed in infected pig lungs . AAB, amino acid biosynthesis. BCPGC, biosynthesis of cofactors, prosthetic groups and carriers. CE, cell enveloppe. CP, cellular process. CIM, central intermediary metabolism. DM, DNA metabolism. EM, energy metabolism. FAPM, fatty acid and phospholipid metabolism. HUU, hypothetical/unclassified/unknown. MEE, mobile extrachromosomal element functions. PF, protein fate. PS, protein synthesis. PPNN, purines, pyrimidins, nucleosides and nucleotides. RF, regulatory functions. ST, signal transduction. Tr, transcription. TBP, transport and binding proteins.

Article Snippet: The A. pleuropneumoniae field strain 896-07 was isolated directly from the infected lung tissues and was routinely grown in BHI medium (Difco) supplemented with either 15 μg/ml (agar) or 5 μg/ml (broth) of NAD.

Techniques: Functional Assay, Infection, Transduction, Binding Assay

Journal: BMC Genomics

Article Title: Microarray-based comparative genomic profiling of reference strains and selected Canadian field isolates of Actinobacillus pleuropneumoniae

doi: 10.1186/1471-2164-10-88

Figure Lengend Snippet: Hierarchical clustering of A. pleuropneumoniae reference strains based on M-CGH data excluding phage and transposase genes . The dendrogram was produced using the MEV software from the J. Craig Venter Institute with Euclidean distance and average linkage clustering (n = 1000 bootstrap iterations).

Article Snippet: The microarrays for the A. pleuropneumoniae field strains were scanned with a Perkin-Elmer ScanArray Express scanner according to the manufacturer's recommendations.

Techniques: Produced, Software

Journal: BMC Genomics

Article Title: Microarray-based comparative genomic profiling of reference strains and selected Canadian field isolates of Actinobacillus pleuropneumoniae

doi: 10.1186/1471-2164-10-88

Figure Lengend Snippet: Variability of genes involved in toxin and capsule biosynthesis across the A. pleuropneumoniae reference strains representing the 15 serovars . Green indicates that the gene is divergent in sequence or absent in the tester strain.

Article Snippet: The microarrays for the A. pleuropneumoniae field strains were scanned with a Perkin-Elmer ScanArray Express scanner according to the manufacturer's recommendations.

Techniques: Sequencing

Journal: BMC Genomics

Article Title: Microarray-based comparative genomic profiling of reference strains and selected Canadian field isolates of Actinobacillus pleuropneumoniae

doi: 10.1186/1471-2164-10-88

Figure Lengend Snippet: Number of strains from 15 fresh field isolates of A. pleuropneumoniae serovars 1, 7 and 15 where the sequence is divergent or absent for each gene, ordered as in A. pleuropneumoniae L20 genome sequence and based on M-CGH results .

Article Snippet: The microarrays for the A. pleuropneumoniae field strains were scanned with a Perkin-Elmer ScanArray Express scanner according to the manufacturer's recommendations.

Techniques: Sequencing

Journal: BMC Genomics

Article Title: Microarray-based comparative genomic profiling of reference strains and selected Canadian field isolates of Actinobacillus pleuropneumoniae

doi: 10.1186/1471-2164-10-88

Figure Lengend Snippet: Hierarchical clustering of field isolates of A. pleuropneumoniae based on M-CGH data excluding phage and transposase genes .

Article Snippet: The microarrays for the A. pleuropneumoniae field strains were scanned with a Perkin-Elmer ScanArray Express scanner according to the manufacturer's recommendations.

Techniques:

Journal: BMC Genomics

Article Title: Microarray-based comparative genomic profiling of reference strains and selected Canadian field isolates of Actinobacillus pleuropneumoniae

doi: 10.1186/1471-2164-10-88

Figure Lengend Snippet: In silico predicted OM proteins and lipoproteins from A. pleuropneumoniae (according to Chung et al. 2007), which are conserved amongst the 15 reference strains and the 21 field isolates tested by M-CGH in this study.

Article Snippet: The microarrays for the A. pleuropneumoniae field strains were scanned with a Perkin-Elmer ScanArray Express scanner according to the manufacturer's recommendations.

Techniques: In Silico, Membrane, Binding Assay, Solvent

Journal: BMC Genomics

Article Title: Microarray-based comparative genomic profiling of reference strains and selected Canadian field isolates of Actinobacillus pleuropneumoniae

doi: 10.1186/1471-2164-10-88

Figure Lengend Snippet: Comparison between M-CGH Log2Ratio data from A. pleuropneumoniae serovar 1 versus serovar 5b hybridizations independently collected, scanned and processed using either ArrayPro software or SpotFinder/MIDAS software . At least 2 independent hybridizations were performed for each dataset, and the processed normalized data was averaged between replicate experiments and between pairs of duplicate spots on the microarray.

Article Snippet: The microarrays for the A. pleuropneumoniae field strains were scanned with a Perkin-Elmer ScanArray Express scanner according to the manufacturer's recommendations.

Techniques: Comparison, Software, Microarray