aspergillus fumigatus  (ATCC)


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    Name:
    Aspergillus fumigatus
    Description:
    Applications Biomedical Research and Development MaterialEmerging infectious disease research
    Catalog Number:
    26933
    Price:
    None
    Applications:
    Biomedical Research and Development MaterialEmerging infectious disease research
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    Structured Review

    ATCC aspergillus fumigatus
    Growth of P. aeruginosa (CFU/ml) cultured in Czapek-Dox media for 48 h compared with growth of P. aeruginosa cultured in Czapek-Dox media supplemented with P. aeruginosa CuF, A. <t>fumigatus</t> CuF and Co-culture CuF. Changes in bacterial growth was greatest where P. aeruginosa was exposed to Co-culture CuF. ***: p
    Applications Biomedical Research and Development MaterialEmerging infectious disease research
    https://www.bioz.com/result/aspergillus fumigatus/product/ATCC
    Average 93 stars, based on 1 article reviews
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    aspergillus fumigatus - by Bioz Stars, 2021-09
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    1) Product Images from "The Aspergillus fumigatus Secretome Alters the Proteome of Pseudomonas aeruginosa to Stimulate Bacterial Growth: Implications for Co-infection"

    Article Title: The Aspergillus fumigatus Secretome Alters the Proteome of Pseudomonas aeruginosa to Stimulate Bacterial Growth: Implications for Co-infection

    Journal: Molecular & Cellular Proteomics : MCP

    doi: 10.1074/mcp.RA120.002059

    Growth of P. aeruginosa (CFU/ml) cultured in Czapek-Dox media for 48 h compared with growth of P. aeruginosa cultured in Czapek-Dox media supplemented with P. aeruginosa CuF, A. fumigatus CuF and Co-culture CuF. Changes in bacterial growth was greatest where P. aeruginosa was exposed to Co-culture CuF. ***: p
    Figure Legend Snippet: Growth of P. aeruginosa (CFU/ml) cultured in Czapek-Dox media for 48 h compared with growth of P. aeruginosa cultured in Czapek-Dox media supplemented with P. aeruginosa CuF, A. fumigatus CuF and Co-culture CuF. Changes in bacterial growth was greatest where P. aeruginosa was exposed to Co-culture CuF. ***: p

    Techniques Used: Cell Culture, Co-Culture Assay

    Volcano plots derived from pairwise comparisons between P. aeruginosa cultured in ( A ) A. fumigatus CuF and P. aeruginosa CuF, ( B ) Co-culture CuF and A. fumigatus CuF and C ) Co-culture CuF and P. aeruginosa CuF. The distribution of quantified proteins according to the p value (- log10 p value) and fold change (log 2 mean LFQ intensity difference) are shown. Proteins above the line are considered statistically significant ( p value
    Figure Legend Snippet: Volcano plots derived from pairwise comparisons between P. aeruginosa cultured in ( A ) A. fumigatus CuF and P. aeruginosa CuF, ( B ) Co-culture CuF and A. fumigatus CuF and C ) Co-culture CuF and P. aeruginosa CuF. The distribution of quantified proteins according to the p value (- log10 p value) and fold change (log 2 mean LFQ intensity difference) are shown. Proteins above the line are considered statistically significant ( p value

    Techniques Used: Derivative Assay, Cell Culture, Co-Culture Assay

    A , Principal component analysis (PCA) of P. aeruginosa exposed to Co-culture CuF (green) A. fumigatus CuF (orange) and P. aeruginosa CuF (blue). A clear distinction can be observed between each of the groups. B . Clusters based on protein-abundance profile similarities were resolved by hierarchical clustering of multi-sample comparisons between the three sample groups of P. aeruginosa . Nine clusters (A–I) were resolved comprising proteins that display similar expression profiles across treatments. Of these, six clusters (A, C, D, G–I) had statistically enriched Gene Ontology (GO) and KEGG terms associated with them ( supplemental Data set S3 ) and the main terms are summarized for each in C .
    Figure Legend Snippet: A , Principal component analysis (PCA) of P. aeruginosa exposed to Co-culture CuF (green) A. fumigatus CuF (orange) and P. aeruginosa CuF (blue). A clear distinction can be observed between each of the groups. B . Clusters based on protein-abundance profile similarities were resolved by hierarchical clustering of multi-sample comparisons between the three sample groups of P. aeruginosa . Nine clusters (A–I) were resolved comprising proteins that display similar expression profiles across treatments. Of these, six clusters (A, C, D, G–I) had statistically enriched Gene Ontology (GO) and KEGG terms associated with them ( supplemental Data set S3 ) and the main terms are summarized for each in C .

    Techniques Used: Co-Culture Assay, Expressing

    A , Protein hydrolysate analysis performed on A. fumigatus CuF produced at 48 h detected seven amino acids by RP-HPLC including aspartic acid, glutamic acid, serine, threonine, methionine, valine and leucine. B , P. aeruginosa (24-hour cultures) were exposed to un-supplemented Czapek-Dox or Czapek-Dox supplemented with the seven amino acids detected in the A. fumigatus CuF hyrolysates. P. aeruginosa growth (CFU/ml) increased by 1.6-fold when cultured in amino acid-supplemented Czapek-Dox for 24 h compared with that of bacteria exposed to un-supplemented (- amino acids) Czapek-Dox for 24 h, where growth increased by 1.17-fold.
    Figure Legend Snippet: A , Protein hydrolysate analysis performed on A. fumigatus CuF produced at 48 h detected seven amino acids by RP-HPLC including aspartic acid, glutamic acid, serine, threonine, methionine, valine and leucine. B , P. aeruginosa (24-hour cultures) were exposed to un-supplemented Czapek-Dox or Czapek-Dox supplemented with the seven amino acids detected in the A. fumigatus CuF hyrolysates. P. aeruginosa growth (CFU/ml) increased by 1.6-fold when cultured in amino acid-supplemented Czapek-Dox for 24 h compared with that of bacteria exposed to un-supplemented (- amino acids) Czapek-Dox for 24 h, where growth increased by 1.17-fold.

    Techniques Used: Produced, High Performance Liquid Chromatography, Cell Culture

    A , Changes in growth of a 24-hour P. aeruginosa culture incubated with sterile Czapek-Dox media (control) or 24-hour, 48-hour, 72-hour or 96-hour A. fumigatus wild-type CuF for 24 h. Maximum growth increase was observed in bacteria exposed to the 48-hour CuF and growth inhibition was observed in bacteria incubated with 96-hour CuF. B , Changes in growth of a 24-hour P. aeruginosa culture incubated in sterile Czapek-Dox media, or 24-hour, 48-hour, 72-hour or 96-hour A. fumigatus Δ giZ CuF for 24 h. Growth was not inhibited by the 96-hour Δ gliZ CuF.
    Figure Legend Snippet: A , Changes in growth of a 24-hour P. aeruginosa culture incubated with sterile Czapek-Dox media (control) or 24-hour, 48-hour, 72-hour or 96-hour A. fumigatus wild-type CuF for 24 h. Maximum growth increase was observed in bacteria exposed to the 48-hour CuF and growth inhibition was observed in bacteria incubated with 96-hour CuF. B , Changes in growth of a 24-hour P. aeruginosa culture incubated in sterile Czapek-Dox media, or 24-hour, 48-hour, 72-hour or 96-hour A. fumigatus Δ giZ CuF for 24 h. Growth was not inhibited by the 96-hour Δ gliZ CuF.

    Techniques Used: Incubation, Inhibition

    Related Articles

    Concentration Assay:

    Article Title: The Aspergillus fumigatus Secretome Alters the Proteome of Pseudomonas aeruginosa to Stimulate Bacterial Growth: Implications for Co-infection
    Article Snippet: .. The concentration of gliotoxin produced by A. fumigatus ATCC 26933 and ΔgliZ in Czapek-Dox at different time-points was detected by RP-HPLC. ..

    Produced:

    Article Title: The Aspergillus fumigatus Secretome Alters the Proteome of Pseudomonas aeruginosa to Stimulate Bacterial Growth: Implications for Co-infection
    Article Snippet: .. The concentration of gliotoxin produced by A. fumigatus ATCC 26933 and ΔgliZ in Czapek-Dox at different time-points was detected by RP-HPLC. ..

    other:

    Article Title: Interplay between Gliotoxin Resistance, Secretion, and the Methyl/Methionine Cycle in Aspergillus fumigatus
    Article Snippet: Indeed, it is clear that cellular SAH levels are most significantly increased in the Δ gliT ATCC 46645 and Δ gliT ATCC 26933 strains ( P = 0.0135 and P = 0.0002, respectively), with significant depletion of SAM levels in the Δ gliT ATCC 46645 strain ( P = 0.0359) and the Δ gliT ATCC 26933 strain ( P = 0.0028) ( and ), the gli single-deletion strain whose growth is most inhibited by exogenous gliotoxin, compared to all other strains tested. gliT complementation restores the wild-type scenario ( and ).

    Article Title: Interplay between Gliotoxin Resistance, Secretion, and the Methyl/Methionine Cycle in Aspergillus fumigatus
    Article Snippet: Following 2D-PAGE, 33 protein spots displayed significant changes in abundance in the A. fumigatus Δ gliK ATCC 26933 strain (19 with ≥1.5-fold-increased and 14 with ≥1.5-fold-decreased abundances; P < 0.05) ( ) upon exposure to gliotoxin (10 μg/ml) for 4 h ( and and ).

    Evaporation:

    Article Title: The Aspergillus fumigatus Secretome Alters the Proteome of Pseudomonas aeruginosa to Stimulate Bacterial Growth: Implications for Co-infection
    Article Snippet: .. Extraction of Gliotoxin from A. fumigatus Culture Filtrate The CuF of A. fumigatus ATCC 26933 type and ΔgliZ strains were mixed with an equal volume (20 ml) chloroform (Fisher Scientific) and mixed for 2 h. The chloroform fraction was collected and dried by rotary evaporation in a Büchi rotor evaporator (Brinkmann Instruments; Westbury, NY). ..

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    ATCC a fumigatus
    Iron saturation of transferrin following incubation with A. <t>fumigatus</t> siderophores. Purified desferrisiderophores were incubated with holotransferrin (25 μM) at 37°C for 16 h. Desferriferrichrome, desferriferricrocin, and desferritriacetylfusarinine C were serially diluted to final concentrations of 5 μM (lanes 1), 50 μM (lanes 2), 500 μM (lanes 3), and 5 mM (lanes 4). Controls containing holotransferrin alone also were run (lanes 0). holo-Tf, holotransferrin; Fe-Tf, monoferric transferrin; apo-Tf, apotransferrin.
    A Fumigatus, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Iron saturation of transferrin following incubation with A. fumigatus siderophores. Purified desferrisiderophores were incubated with holotransferrin (25 μM) at 37°C for 16 h. Desferriferrichrome, desferriferricrocin, and desferritriacetylfusarinine C were serially diluted to final concentrations of 5 μM (lanes 1), 50 μM (lanes 2), 500 μM (lanes 3), and 5 mM (lanes 4). Controls containing holotransferrin alone also were run (lanes 0). holo-Tf, holotransferrin; Fe-Tf, monoferric transferrin; apo-Tf, apotransferrin.

    Journal: Infection and Immunity

    Article Title: Survival of Aspergillus fumigatus in Serum Involves Removal of Iron from Transferrin: the Role of Siderophores

    doi: 10.1128/IAI.72.3.1402-1408.2004

    Figure Lengend Snippet: Iron saturation of transferrin following incubation with A. fumigatus siderophores. Purified desferrisiderophores were incubated with holotransferrin (25 μM) at 37°C for 16 h. Desferriferrichrome, desferriferricrocin, and desferritriacetylfusarinine C were serially diluted to final concentrations of 5 μM (lanes 1), 50 μM (lanes 2), 500 μM (lanes 3), and 5 mM (lanes 4). Controls containing holotransferrin alone also were run (lanes 0). holo-Tf, holotransferrin; Fe-Tf, monoferric transferrin; apo-Tf, apotransferrin.

    Article Snippet: This conclusion was further supported by results showing that A. fumigatus was able to grow in MEM containing transferrin alone without any degradation of transferrin.

    Techniques: Incubation, Purification

    Iron removal from transferrin by A. fumigatus. A. fumigatus was cultured in MEM containing 2.5 μM purified human holotransferrin (A) or 10% human serum (B). Culture media were withdrawn, and the iron saturation of transferrin was analyzed by urea-PAGE. Transferrin was visualized by Western blotting. The numbers above the lanes represent the hours of incubation with A. fumigatus . Fe 2 -Tf, holotransferrin; Apo-Tf, apotransferrin.

    Journal: Infection and Immunity

    Article Title: Survival of Aspergillus fumigatus in Serum Involves Removal of Iron from Transferrin: the Role of Siderophores

    doi: 10.1128/IAI.72.3.1402-1408.2004

    Figure Lengend Snippet: Iron removal from transferrin by A. fumigatus. A. fumigatus was cultured in MEM containing 2.5 μM purified human holotransferrin (A) or 10% human serum (B). Culture media were withdrawn, and the iron saturation of transferrin was analyzed by urea-PAGE. Transferrin was visualized by Western blotting. The numbers above the lanes represent the hours of incubation with A. fumigatus . Fe 2 -Tf, holotransferrin; Apo-Tf, apotransferrin.

    Article Snippet: This conclusion was further supported by results showing that A. fumigatus was able to grow in MEM containing transferrin alone without any degradation of transferrin.

    Techniques: Cell Culture, Purification, Polyacrylamide Gel Electrophoresis, Western Blot, Incubation

    Degradation of transferrin by A. fumigatus in liquid cultures. A. fumigatus was incubated in MEM containing 10% human serum (A) or 2.5 μM holotransferrin (B). Supernatants were withdrawn from the cultures after the number of hours indicated above the lanes, and the presence of transferrin was determined by Western blotting following sodium dodecyl sulfate-PAGE. Controls (lanes C) were uninoculated samples incubated for 286 h. The band underneath transferrin in panel A is another protein that cross-reacted with the polyclonal antitransferrin antibody.

    Journal: Infection and Immunity

    Article Title: Survival of Aspergillus fumigatus in Serum Involves Removal of Iron from Transferrin: the Role of Siderophores

    doi: 10.1128/IAI.72.3.1402-1408.2004

    Figure Lengend Snippet: Degradation of transferrin by A. fumigatus in liquid cultures. A. fumigatus was incubated in MEM containing 10% human serum (A) or 2.5 μM holotransferrin (B). Supernatants were withdrawn from the cultures after the number of hours indicated above the lanes, and the presence of transferrin was determined by Western blotting following sodium dodecyl sulfate-PAGE. Controls (lanes C) were uninoculated samples incubated for 286 h. The band underneath transferrin in panel A is another protein that cross-reacted with the polyclonal antitransferrin antibody.

    Article Snippet: This conclusion was further supported by results showing that A. fumigatus was able to grow in MEM containing transferrin alone without any degradation of transferrin.

    Techniques: Incubation, Western Blot, Polyacrylamide Gel Electrophoresis

    Siderophore secretion by A. fumigatus in MEM containing holotransferrin or human serum. A. fumigatus spores (10 6 /ml) were added to MEM containing 2.5 μM holotransferrin (holo-Tf) or 10% human serum at time zero. The cultures were incubated at 37°C and 150 rpm. The siderophore concentrations in the culture supernatants were determined by the CAS assay. Error bars indicate standard deviations.

    Journal: Infection and Immunity

    Article Title: Survival of Aspergillus fumigatus in Serum Involves Removal of Iron from Transferrin: the Role of Siderophores

    doi: 10.1128/IAI.72.3.1402-1408.2004

    Figure Lengend Snippet: Siderophore secretion by A. fumigatus in MEM containing holotransferrin or human serum. A. fumigatus spores (10 6 /ml) were added to MEM containing 2.5 μM holotransferrin (holo-Tf) or 10% human serum at time zero. The cultures were incubated at 37°C and 150 rpm. The siderophore concentrations in the culture supernatants were determined by the CAS assay. Error bars indicate standard deviations.

    Article Snippet: This conclusion was further supported by results showing that A. fumigatus was able to grow in MEM containing transferrin alone without any degradation of transferrin.

    Techniques: Incubation

    Localization of A. fumigatus conidia within the airway epithelial cell monolayer. GFP-expressing A. fumigatus conidia and primary AECs were co-incubated for 6 hours and treated with DAPI and monoclonal E-cadherin Alexa 594 antibody before visualization by confocal microscopy. Labeling of nuclei (blue) and the membrane tight junctional protein E-cadherin (red) allowed visualization of AECs. Some GFP-expressing A. fumigatus conidia (green) are found outside the cells, while others localize within the cell monolayer, in close association with AEC nuclei.

    Journal: PLoS ONE

    Article Title: Dual Organism Transcriptomics of Airway Epithelial Cells Interacting with Conidia of Aspergillus fumigatus

    doi: 10.1371/journal.pone.0020527

    Figure Lengend Snippet: Localization of A. fumigatus conidia within the airway epithelial cell monolayer. GFP-expressing A. fumigatus conidia and primary AECs were co-incubated for 6 hours and treated with DAPI and monoclonal E-cadherin Alexa 594 antibody before visualization by confocal microscopy. Labeling of nuclei (blue) and the membrane tight junctional protein E-cadherin (red) allowed visualization of AECs. Some GFP-expressing A. fumigatus conidia (green) are found outside the cells, while others localize within the cell monolayer, in close association with AEC nuclei.

    Article Snippet: SkpA was shown to be up-regulated in mature biofilms of A. fumigatus , and may represent a requirement for methionine and cysteine-rich proteins during exposure to epithelial cells .

    Techniques: Expressing, Incubation, Confocal Microscopy, Labeling

    Relative mRNA expression levels of human genes obtained by RT-qPCR. RNA was obtained from four co-incubations each of 16HBE14o- (grey bars) or primary normal bronchial epithelial cells (AECs) (white bars) with conidia of A. fumigatus . Height of each bar represents expression of gene in co-incubated condition relative to cells alone control (mean ± SE). (* p

    Journal: PLoS ONE

    Article Title: Dual Organism Transcriptomics of Airway Epithelial Cells Interacting with Conidia of Aspergillus fumigatus

    doi: 10.1371/journal.pone.0020527

    Figure Lengend Snippet: Relative mRNA expression levels of human genes obtained by RT-qPCR. RNA was obtained from four co-incubations each of 16HBE14o- (grey bars) or primary normal bronchial epithelial cells (AECs) (white bars) with conidia of A. fumigatus . Height of each bar represents expression of gene in co-incubated condition relative to cells alone control (mean ± SE). (* p

    Article Snippet: SkpA was shown to be up-regulated in mature biofilms of A. fumigatus , and may represent a requirement for methionine and cysteine-rich proteins during exposure to epithelial cells .

    Techniques: Expressing, Quantitative RT-PCR, Incubation

    Relative mRNA expression levels of A. fumigatus genes as determined by RT-qPCR. RNA was obtained from four co-incubations each of A. fumigatus conidia with 16HBE14o- (grey bars) or primary human bronchial epithelial cells (AECs) (white bars). Height of each bar represents expression of gene in co-incubated condition relative to conida alone control (mean ± SE). (* p

    Journal: PLoS ONE

    Article Title: Dual Organism Transcriptomics of Airway Epithelial Cells Interacting with Conidia of Aspergillus fumigatus

    doi: 10.1371/journal.pone.0020527

    Figure Lengend Snippet: Relative mRNA expression levels of A. fumigatus genes as determined by RT-qPCR. RNA was obtained from four co-incubations each of A. fumigatus conidia with 16HBE14o- (grey bars) or primary human bronchial epithelial cells (AECs) (white bars). Height of each bar represents expression of gene in co-incubated condition relative to conida alone control (mean ± SE). (* p

    Article Snippet: SkpA was shown to be up-regulated in mature biofilms of A. fumigatus , and may represent a requirement for methionine and cysteine-rich proteins during exposure to epithelial cells .

    Techniques: Expressing, Quantitative RT-PCR, Incubation

    Expression of CD62P (A) and CD63 (B) in platelets upon stimulation with conidia (left), swollen conidia (center), and hyphae (right) from Aspergillus fumigatus as assessed by flow cytometry after incubation for 120 min.

    Journal: Infection and Immunity

    Article Title: Activation of Platelets by Aspergillus fumigatus and Potential Role of Platelets in the Immunopathogenesis of Aspergillosis ▿

    doi: 10.1128/IAI.01091-09

    Figure Lengend Snippet: Expression of CD62P (A) and CD63 (B) in platelets upon stimulation with conidia (left), swollen conidia (center), and hyphae (right) from Aspergillus fumigatus as assessed by flow cytometry after incubation for 120 min.

    Article Snippet: Monocytic phagocytes play an important role in the innate immune response to A. fumigatus by ingesting and inactivating conidia.

    Techniques: Expressing, Flow Cytometry, Cytometry, Incubation

    Release of IL-8 from THP-1 monocytes after culture for 4 h with or without unstimulated PRP, hyphae from Aspergillus fumigatus , or both. Data are given as means ± SEM ( n = 11). *, P

    Journal: Infection and Immunity

    Article Title: Activation of Platelets by Aspergillus fumigatus and Potential Role of Platelets in the Immunopathogenesis of Aspergillosis ▿

    doi: 10.1128/IAI.01091-09

    Figure Lengend Snippet: Release of IL-8 from THP-1 monocytes after culture for 4 h with or without unstimulated PRP, hyphae from Aspergillus fumigatus , or both. Data are given as means ± SEM ( n = 11). *, P

    Article Snippet: Monocytic phagocytes play an important role in the innate immune response to A. fumigatus by ingesting and inactivating conidia.

    Techniques:

    Expression of CD62P (A) and CD63 (B) in unstimulated (Us) platelets (left) compared with that in platelets stimulated with hyphae from Aspergillus fumigatus (center) and with that in hyphae separated from platelets with cell culture inserts (right) as

    Journal: Infection and Immunity

    Article Title: Activation of Platelets by Aspergillus fumigatus and Potential Role of Platelets in the Immunopathogenesis of Aspergillosis ▿

    doi: 10.1128/IAI.01091-09

    Figure Lengend Snippet: Expression of CD62P (A) and CD63 (B) in unstimulated (Us) platelets (left) compared with that in platelets stimulated with hyphae from Aspergillus fumigatus (center) and with that in hyphae separated from platelets with cell culture inserts (right) as

    Article Snippet: Monocytic phagocytes play an important role in the innate immune response to A. fumigatus by ingesting and inactivating conidia.

    Techniques: Expressing, Cell Culture

    Release of IL-8 from human monocytes after culture for 4 h with or without unstimulated PRP, hyphae from Aspergillus fumigatus , or both. Data are given as means ± SEM ( n = 6). *, P

    Journal: Infection and Immunity

    Article Title: Activation of Platelets by Aspergillus fumigatus and Potential Role of Platelets in the Immunopathogenesis of Aspergillosis ▿

    doi: 10.1128/IAI.01091-09

    Figure Lengend Snippet: Release of IL-8 from human monocytes after culture for 4 h with or without unstimulated PRP, hyphae from Aspergillus fumigatus , or both. Data are given as means ± SEM ( n = 6). *, P

    Article Snippet: Monocytic phagocytes play an important role in the innate immune response to A. fumigatus by ingesting and inactivating conidia.

    Techniques:

    Instrument repeatability as a function of cell quantity COV ( n = 7). The qPCR MDLs are 5 cells for B. atrophaeus and 0.05 cells for A. fumigatus .

    Journal: Applied and Environmental Microbiology

    Article Title: Accuracy, Precision, and Method Detection Limits of Quantitative PCR for Airborne Bacteria and Fungi ▿

    doi: 10.1128/AEM.01240-10

    Figure Lengend Snippet: Instrument repeatability as a function of cell quantity COV ( n = 7). The qPCR MDLs are 5 cells for B. atrophaeus and 0.05 cells for A. fumigatus .

    Article Snippet: The percent extraction efficiency, ηDNA , and standard deviations for five independent experiments, from highest to lowest, included the following: for E. coli , η DNA of 16.3% ± 2.1%; for A. fumigatus , η DNA of 8.8% ± 0.7%; and for B. atrophaeus , η DNA of 8.3% ± 1.0%.

    Techniques: Real-time Polymerase Chain Reaction