a actinomycetemcomitans cdt  (ATCC)


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    Structured Review

    ATCC a actinomycetemcomitans cdt
    Genes linked to different phenotypes A. Gene trap insertions identified in loci essential for <t>CDT</t> intoxication. A color code distinguishes gene trap insertions that were enriched using distinct CDTs: A. <t>actinomycetemcomitans</t> in green, E. coli in red, H. ducreyi in yellow and C. jejuni in blue. B. Loci linked to 12 separate phenotypes. Cells were exposed to small molecule inhibitors of the Bcl2-family (ABT-737), chk1-kinase activity (AZD7762), Bcr-Abl-activity (imatinib), or DNA methylation (decitabine). Additional phenotypic selections were done using biological agents including TRAIL, CDTs, diphtheria toxin, ricin toxin and reovirus. Gene trap insertions in exonic sequences or in the sense orientation of genes were counted per individual selection. Enrichment p-value was calculated for each gene locus by comparing this number to the number of insertions identified in the same locus within the unselected cell population. Each screen resulted in a distinct set of between 1 and 8 genes with high significance. These include already known entry factors used by pathogens such as the entry receptor for diphtheria toxin (HBEGF), the reovirus receptor (F11R) and an enzyme involved in carbohydrate synthesis required for ricin entry (MGAT2). It also includes downstream effectors of kinases for example CDC25A in a screen with a Chk1 inhibitor or PTPN1 and PTPN12 identified by BCR-ABL inhibition using imatinib. Strong resistance against decitabine was observed in cells containing mutations in deoxycytidine kinase (DCK), the rate-limiting kinase for activation of several nucleoside analogs 20 .
    A Actinomycetemcomitans Cdt, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Related Products / Commonly Used Together

    subunits cdta
    cdtc
    aggregatibacter actinomycetemcomitans

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    1) Product Images from "Global gene disruption in human cells to assign genes to phenotypes"

    Article Title: Global gene disruption in human cells to assign genes to phenotypes

    Journal: Nature biotechnology

    doi: 10.1038/nbt.1857

    Genes linked to different phenotypes A. Gene trap insertions identified in loci essential for CDT intoxication. A color code distinguishes gene trap insertions that were enriched using distinct CDTs: A. actinomycetemcomitans in green, E. coli in red, H. ducreyi in yellow and C. jejuni in blue. B. Loci linked to 12 separate phenotypes. Cells were exposed to small molecule inhibitors of the Bcl2-family (ABT-737), chk1-kinase activity (AZD7762), Bcr-Abl-activity (imatinib), or DNA methylation (decitabine). Additional phenotypic selections were done using biological agents including TRAIL, CDTs, diphtheria toxin, ricin toxin and reovirus. Gene trap insertions in exonic sequences or in the sense orientation of genes were counted per individual selection. Enrichment p-value was calculated for each gene locus by comparing this number to the number of insertions identified in the same locus within the unselected cell population. Each screen resulted in a distinct set of between 1 and 8 genes with high significance. These include already known entry factors used by pathogens such as the entry receptor for diphtheria toxin (HBEGF), the reovirus receptor (F11R) and an enzyme involved in carbohydrate synthesis required for ricin entry (MGAT2). It also includes downstream effectors of kinases for example CDC25A in a screen with a Chk1 inhibitor or PTPN1 and PTPN12 identified by BCR-ABL inhibition using imatinib. Strong resistance against decitabine was observed in cells containing mutations in deoxycytidine kinase (DCK), the rate-limiting kinase for activation of several nucleoside analogs 20 .
    Figure Legend Snippet: Genes linked to different phenotypes A. Gene trap insertions identified in loci essential for CDT intoxication. A color code distinguishes gene trap insertions that were enriched using distinct CDTs: A. actinomycetemcomitans in green, E. coli in red, H. ducreyi in yellow and C. jejuni in blue. B. Loci linked to 12 separate phenotypes. Cells were exposed to small molecule inhibitors of the Bcl2-family (ABT-737), chk1-kinase activity (AZD7762), Bcr-Abl-activity (imatinib), or DNA methylation (decitabine). Additional phenotypic selections were done using biological agents including TRAIL, CDTs, diphtheria toxin, ricin toxin and reovirus. Gene trap insertions in exonic sequences or in the sense orientation of genes were counted per individual selection. Enrichment p-value was calculated for each gene locus by comparing this number to the number of insertions identified in the same locus within the unselected cell population. Each screen resulted in a distinct set of between 1 and 8 genes with high significance. These include already known entry factors used by pathogens such as the entry receptor for diphtheria toxin (HBEGF), the reovirus receptor (F11R) and an enzyme involved in carbohydrate synthesis required for ricin entry (MGAT2). It also includes downstream effectors of kinases for example CDC25A in a screen with a Chk1 inhibitor or PTPN1 and PTPN12 identified by BCR-ABL inhibition using imatinib. Strong resistance against decitabine was observed in cells containing mutations in deoxycytidine kinase (DCK), the rate-limiting kinase for activation of several nucleoside analogs 20 .

    Techniques Used: Activity Assay, DNA Methylation Assay, Selection, Inhibition, Activation Assay

    Related Articles

    Sequencing:

    Article Title: Global gene disruption in human cells to assign genes to phenotypes
    Article Snippet: .. A. actinomycetemcomitans CDT The operon sequence between subunits CdtA and CdtC was amplified from genomic DNA of Aggregatibacter actinomycetemcomitans (ATCC 700685D-5) using the following forward: 5′-ATCTAAGGAGAGGTACAATGAAA-3′ and reverse 5′-TTAGCTACCCTGATTTCTCC-3′ primers. .. The PCR product was purified from agarose gel using a QIAquick Gel Extraction Kit (Qiagen) and cloned into the pGemTeasy vector (Promega).

    Amplification:

    Article Title: Global gene disruption in human cells to assign genes to phenotypes
    Article Snippet: .. A. actinomycetemcomitans CDT The operon sequence between subunits CdtA and CdtC was amplified from genomic DNA of Aggregatibacter actinomycetemcomitans (ATCC 700685D-5) using the following forward: 5′-ATCTAAGGAGAGGTACAATGAAA-3′ and reverse 5′-TTAGCTACCCTGATTTCTCC-3′ primers. .. The PCR product was purified from agarose gel using a QIAquick Gel Extraction Kit (Qiagen) and cloned into the pGemTeasy vector (Promega).

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    ATCC cdt locus
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    ATCC a actinomycetemcomitans cdt
    Genes linked to different phenotypes A. Gene trap insertions identified in loci essential for <t>CDT</t> intoxication. A color code distinguishes gene trap insertions that were enriched using distinct CDTs: A. <t>actinomycetemcomitans</t> in green, E. coli in red, H. ducreyi in yellow and C. jejuni in blue. B. Loci linked to 12 separate phenotypes. Cells were exposed to small molecule inhibitors of the Bcl2-family (ABT-737), chk1-kinase activity (AZD7762), Bcr-Abl-activity (imatinib), or DNA methylation (decitabine). Additional phenotypic selections were done using biological agents including TRAIL, CDTs, diphtheria toxin, ricin toxin and reovirus. Gene trap insertions in exonic sequences or in the sense orientation of genes were counted per individual selection. Enrichment p-value was calculated for each gene locus by comparing this number to the number of insertions identified in the same locus within the unselected cell population. Each screen resulted in a distinct set of between 1 and 8 genes with high significance. These include already known entry factors used by pathogens such as the entry receptor for diphtheria toxin (HBEGF), the reovirus receptor (F11R) and an enzyme involved in carbohydrate synthesis required for ricin entry (MGAT2). It also includes downstream effectors of kinases for example CDC25A in a screen with a Chk1 inhibitor or PTPN1 and PTPN12 identified by BCR-ABL inhibition using imatinib. Strong resistance against decitabine was observed in cells containing mutations in deoxycytidine kinase (DCK), the rate-limiting kinase for activation of several nucleoside analogs 20 .
    A Actinomycetemcomitans Cdt, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a actinomycetemcomitans cdt/product/ATCC
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a actinomycetemcomitans cdt - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

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    Genes linked to different phenotypes A. Gene trap insertions identified in loci essential for CDT intoxication. A color code distinguishes gene trap insertions that were enriched using distinct CDTs: A. actinomycetemcomitans in green, E. coli in red, H. ducreyi in yellow and C. jejuni in blue. B. Loci linked to 12 separate phenotypes. Cells were exposed to small molecule inhibitors of the Bcl2-family (ABT-737), chk1-kinase activity (AZD7762), Bcr-Abl-activity (imatinib), or DNA methylation (decitabine). Additional phenotypic selections were done using biological agents including TRAIL, CDTs, diphtheria toxin, ricin toxin and reovirus. Gene trap insertions in exonic sequences or in the sense orientation of genes were counted per individual selection. Enrichment p-value was calculated for each gene locus by comparing this number to the number of insertions identified in the same locus within the unselected cell population. Each screen resulted in a distinct set of between 1 and 8 genes with high significance. These include already known entry factors used by pathogens such as the entry receptor for diphtheria toxin (HBEGF), the reovirus receptor (F11R) and an enzyme involved in carbohydrate synthesis required for ricin entry (MGAT2). It also includes downstream effectors of kinases for example CDC25A in a screen with a Chk1 inhibitor or PTPN1 and PTPN12 identified by BCR-ABL inhibition using imatinib. Strong resistance against decitabine was observed in cells containing mutations in deoxycytidine kinase (DCK), the rate-limiting kinase for activation of several nucleoside analogs 20 .

    Journal: Nature biotechnology

    Article Title: Global gene disruption in human cells to assign genes to phenotypes

    doi: 10.1038/nbt.1857

    Figure Lengend Snippet: Genes linked to different phenotypes A. Gene trap insertions identified in loci essential for CDT intoxication. A color code distinguishes gene trap insertions that were enriched using distinct CDTs: A. actinomycetemcomitans in green, E. coli in red, H. ducreyi in yellow and C. jejuni in blue. B. Loci linked to 12 separate phenotypes. Cells were exposed to small molecule inhibitors of the Bcl2-family (ABT-737), chk1-kinase activity (AZD7762), Bcr-Abl-activity (imatinib), or DNA methylation (decitabine). Additional phenotypic selections were done using biological agents including TRAIL, CDTs, diphtheria toxin, ricin toxin and reovirus. Gene trap insertions in exonic sequences or in the sense orientation of genes were counted per individual selection. Enrichment p-value was calculated for each gene locus by comparing this number to the number of insertions identified in the same locus within the unselected cell population. Each screen resulted in a distinct set of between 1 and 8 genes with high significance. These include already known entry factors used by pathogens such as the entry receptor for diphtheria toxin (HBEGF), the reovirus receptor (F11R) and an enzyme involved in carbohydrate synthesis required for ricin entry (MGAT2). It also includes downstream effectors of kinases for example CDC25A in a screen with a Chk1 inhibitor or PTPN1 and PTPN12 identified by BCR-ABL inhibition using imatinib. Strong resistance against decitabine was observed in cells containing mutations in deoxycytidine kinase (DCK), the rate-limiting kinase for activation of several nucleoside analogs 20 .

    Article Snippet: A. actinomycetemcomitans CDT The operon sequence between subunits CdtA and CdtC was amplified from genomic DNA of Aggregatibacter actinomycetemcomitans (ATCC 700685D-5) using the following forward: 5′-ATCTAAGGAGAGGTACAATGAAA-3′ and reverse 5′-TTAGCTACCCTGATTTCTCC-3′ primers.

    Techniques: Activity Assay, DNA Methylation Assay, Selection, Inhibition, Activation Assay