dnaase (Worthington Biochemical)

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Name:

Deoxyribonuclease I

Description:

Chromatographically purified A lyophilized powder with glycine as a stabilizer

Catalog Number:

ls002004

Price:

Size:

5 mg

Source:

Bovine Pancreas

Cas Number:

9003.98.9

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Structured Review

Worthington Biochemical dnaase
Chromatographically purified A lyophilized powder with glycine as a stabilizer
https://www.bioz.com/result/dnaase/product/Worthington Biochemical
Average 99 stars, based on 245 article reviews
Price from $9.99 to $1999.99

dnaase - by Bioz Stars, 2020-05

99/100 stars

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Images

In Vitro:

Article Title: Extracellular traps are associated with human and mouse neutrophil and macrophage mediated killing of larval Strongyloides stercoralis
Article Snippet: .. Treatment with DNase I eliminated the presence of released DNA, but did not block killing of the larvae by mouse neutrophils and macrophages in vitro. .. This observation suggests that in contrast to human neutrophils and macrophages, mouse cells do not require ET formation in vitro to kill the worms.

Positive Control:

Article Title: STAT3, STAT4, NFATc1, and CTCF regulate PD-1 through multiple novel regulatory regions in murine T cells
Article Snippet: .. Concentration ranges for DNase I were determined empirically for each lot and cell type by titrating DNase I for its ability to digest CR-C as the positive control but not regions previously found to be resistant to DNase I (e.g., +6.3 region). .. Following purification of the digested DNA, PCR was performed across the Pdcd1 locus using a set of 59 primer pairs ( ).

Blocking Assay:

Concentration Assay:

Article Title: Interactions of NBU1 IntN1 and Orf2x Proteins with Attachment Site DNA
Article Snippet: .. DNase I (Worthington) diluted in DNase I dilution buffer (2.5 mM MgCl2 , 0.5 mM CaCl2 , 10 mM Tris-HCl, pH 7.6, 0.1 mg/ml BSA) to a final concentration of 0.0625 μg/ml was incubated with reaction mixtures for 1 min. ..

Incubation:

Article Title: Epigenetic Control of Cell Cycle-Dependent Histone Gene Expression Is a Principal Component of the Abbreviated Pluripotent Cell Cycle
Article Snippet: .. Nuclei were then resuspended in RSB buffer supplemented with 1 mM CaCl2 and incubated with increasing concentrations of DNase I (DPRF; Worthington Biochemical Corporation, Lakewood, NJ) for 10 min at room temperature with gentle agitation. ..

other:

Article Title: Interactions of NBU1 IntN1 and Orf2x Proteins with Attachment Site DNA
Article Snippet: The region is dA+dT rich, and protection seen from positions +126 to +129 is subtle because DNase I does not cleave DNA efficiently in this region.

Article Title: Structural and functional conservation at the boundaries of the chicken ?-globin domain
Article Snippet: The DNase I HS, 3′HS, was detected by digestion of nuclei with increasing amounts of DNase I as above followed by digestion of extracted DNA with Kpn I and probing with i112 (1000 bp; Kpn I –Pst I at 23.3–24.3 map units).