dnaase (Worthington Biochemical)

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Name:

Deoxyribonuclease I

Description:

Chromatographically purified A lyophilized powder with glycine as a stabilizer

Catalog Number:

ls002004

Price:

Size:

5 mg

Source:

Bovine Pancreas

Cas Number:

9003.98.9

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Structured Review

Worthington Biochemical dnaase
Chromatographically purified A lyophilized powder with glycine as a stabilizer
https://www.bioz.com/result/dnaase/product/Worthington Biochemical
Average 99 stars, based on 245 article reviews
Price from $9.99 to $1999.99

dnaase - by Bioz Stars, 2020-08

99/100 stars

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Images

Produced:

Article Title: Novel High-Throughput Deoxyribonuclease 1 Assay
Article Snippet: .. The percentage of DNase I activity was calculated using Equation 1: DNase\u00a0I\u00a0activity (%) =\u00a0 (mean\u00a0velocity\u00a0of\u00a0a\u00a0compound/mean\u00a0velocity\u00a0of\u00a0DMSO)\u00a0\u00d7\u00a0100 (1) In similar assays, recombinant murine EndoG (produced in-house) was used at a concentration of 0.14 μM in 0.1 mM MgCl2 , 10 mM Tris-HCl, pH 7.4; and DNase II (Worthington, Lakewood, NJ) (3.32 nM) was tested in 100 mM sodium citrate buffer, pH 5.0. .. For evaluation of the quality of the assay, Z’ values were calculated using Equation 2: Z\u2019 =\u00a01\u00a0\u2212\u00a0(3SDC +\u00a03SDB )/(MC \u00a0\u2212\u00a0MB ) (2) where M = mean value; SD = standard deviation; C = control; and B = background.

Concentration Assay:

other:

Article Title: DNase I aggravates islet β-cell apoptosis in type 2 diabetes
Article Snippet: The role of DNase I in SEL and AMI has been previously investigated, however at present to the best of our knowledge, no correlative studies on its association with type 2 diabetes have been conducted ( , , ).

Activity Assay:

Expressing:

Article Title: DNase I aggravates islet β-cell apoptosis in type 2 diabetes
Article Snippet: .. In order to confirm that apoptosis was correlated with DNase I expression, siRNA was used to knock down DNase I. .. It was observed that a reduction in DNase I expression resulted in significant reductions in apoptotic rate and caspase-3 protein levels, even in the presence of high glucose.

Staining:

Article Title: Constitutive Nucleosome Depletion and Ordered Factor Assembly at the GRP78 Promoter Revealed by Single Molecule Footprinting
Article Snippet: .. These were then digested at 37 °C for 15 min using various concentrations of DNase I (Worthington, San Francisco, California, United States) to obtain a suitable range of digestion of genomic DNA as revealed by EtBr staining. .. Digested genomic DNA was purified, redigested by RsaI, resolved on a 1.5% agarose gel, and Southern blotted.