wrw4  (Alomone Labs)


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    Name:
    WRW4
    Description:
    WRW4 is a selective and potent antagonist of the Formylpeptide receptor FPR2
    Catalog Number:
    GPW-110
    Price:
    67.0
    Category:
    Peptide
    Source:
    Synthetic peptide
    Applications:
    0
    Purity:
    >98% (HPLC)
    Size:
    0 5 mg
    Format:
    Lyophilized powder.
    Formula:
    C61H65N15O6
    Molecular Weight:
    1104.3 Da.
    Molecule Name:
    WRW4, WRW4, Formyl Peptide Receptor - Like 1 (FPRL1) Antagonist
    Buy from Supplier


    Structured Review

    Alomone Labs wrw4
    WRW4
    WRW4 is a selective and potent antagonist of the Formylpeptide receptor FPR2
    https://www.bioz.com/result/wrw4/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wrw4 - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Inhibiting PSMα-induced neutrophil necroptosis protects mice with MRSA pneumonia by blocking the agr system"

    Article Title: Inhibiting PSMα-induced neutrophil necroptosis protects mice with MRSA pneumonia by blocking the agr system

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-018-0398-z

    PSMα1-induced neutrophil necroptosis was blocked by WRW4 or anti-TNFα. a, c Neutrophil were treated with PBS or TNFα neutralizing antibody at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6) and the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3). b, d, e Neutrophil were treated with PBS or WRW4 at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6), the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3), and the level of TNFα was detected by ELISA assay ( n = 8). Data were shown as mean ± SE (a, b, e). * P
    Figure Legend Snippet: PSMα1-induced neutrophil necroptosis was blocked by WRW4 or anti-TNFα. a, c Neutrophil were treated with PBS or TNFα neutralizing antibody at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6) and the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3). b, d, e Neutrophil were treated with PBS or WRW4 at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6), the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3), and the level of TNFα was detected by ELISA assay ( n = 8). Data were shown as mean ± SE (a, b, e). * P

    Techniques Used: Incubation, Western Blot, Enzyme-linked Immunosorbent Assay

    2) Product Images from "Inhibiting PSMα-induced neutrophil necroptosis protects mice with MRSA pneumonia by blocking the agr system"

    Article Title: Inhibiting PSMα-induced neutrophil necroptosis protects mice with MRSA pneumonia by blocking the agr system

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-018-0398-z

    PSMα1-induced neutrophil necroptosis was blocked by WRW4 or anti-TNFα. a, c Neutrophil were treated with PBS or TNFα neutralizing antibody at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6) and the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3). b, d, e Neutrophil were treated with PBS or WRW4 at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6), the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3), and the level of TNFα was detected by ELISA assay ( n = 8). Data were shown as mean ± SE (a, b, e). * P
    Figure Legend Snippet: PSMα1-induced neutrophil necroptosis was blocked by WRW4 or anti-TNFα. a, c Neutrophil were treated with PBS or TNFα neutralizing antibody at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6) and the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3). b, d, e Neutrophil were treated with PBS or WRW4 at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6), the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3), and the level of TNFα was detected by ELISA assay ( n = 8). Data were shown as mean ± SE (a, b, e). * P

    Techniques Used: Incubation, Western Blot, Enzyme-linked Immunosorbent Assay

    Related Articles

    other:

    Article Title: The N-Formyl Peptide Receptor 2 (FPR2) Agonist MR-39 Exhibits Anti-Inflammatory Activity in LPS-Stimulated Organotypic Hippocampal Cultures
    Article Snippet: The FPR2 antagonist WRW4 was purchased from Alomone Labs, Jerusalem, Israel.

    Article Title: Novel Ureidopropanamide Based N-Formyl Peptide Receptor 2 (FPR2) Agonists with Potential Application for Central Nervous System Disorders Characterized by Neuroinflammation
    Article Snippet: Additionally, in experiments where the secretion of NO or cytokines were measured, the FPR2 antagonist WRW4 (Alomone Labs, Israel) was added 30 min before agonists ( ).

    Cell Culture:

    Article Title: Inhibiting PSMα-induced neutrophil necroptosis protects mice with MRSA pneumonia by blocking the agr system
    Article Snippet: .. Neutrophil cells were respectively stimulated with PSM peptide for 1 h at 37 °C and then centrifugated (1000 rpm, 10 min) after pretreated with or without NSA, Nec, WRW4, anti-TNFα for 1 h. The neutrophil cell culture supernatant is used to detect the release of LDH. ..

    Sequencing:

    Article Title: Bacterial lipopolysaccharide and antimicrobial LL-37 enhance ICAM-1 expression and NF-κB p65 phosphorylation in senescent endothelial cells
    Article Snippet: .. The FPR2 antagonist WRW4 peptide (sequence: WRWWWW) was from Alomone Labs; the P2X7 inhibitor KN-62 was from Sigma-Aldrich (Merck KGaA). ..

    Staining:

    Article Title: The Annexin A1 Receptor FPR2 Regulates the Endosomal Export of Influenza Virus
    Article Snippet: .. Reagents Cells and VirusesThe following reagents were used in this study: antiviral M2 protein (Santa Cruz, Heidelberg, Germany), Alexa Fluor secondary antibodies (Life Technologies, Villebon-sur-Yvette, France), DAPI (4′,6′-diamidino-2-phenylindole, Sigma-Aldrich, Darmstadt, Germany), phalloidin (Invitrogen, Villebon-sur-Yvette, France), purple crystal staining (Sigma, Darmstadt, Germany), FPR2 antagonists WRW4 (Alomone Labs, Jerusalem, Israel), monoclonal anti-FPR2 antibody (clone FN-1D6-AI, Genova, Italy), monoclonal anti-Nucleoprotein (NP, HB-65, invivomab), conjugated Alexa Fluor 488 anti-Rab5 antibody (Santa Cruz, Heidelberg, Germany), IgG control antibody (ThermoFisher, Villebon-sur-Yvette, France), ERK inhibitor pathway U0126 (Sigma-Aldrich, Darmstadt, Germany). ..

    Article Title: The Annexin A1 Receptor FPR2 Regulates the Endosomal Export of Influenza Virus
    Article Snippet: .. The following reagents were used in this study: antiviral M2 protein (Santa Cruz, Heidelberg, Germany), Alexa Fluor secondary antibodies (Life Technologies, Villebon-sur-Yvette, France), DAPI (4′,6′-diamidino-2-phenylindole, Sigma-Aldrich, Darmstadt, Germany), phalloidin (Invitrogen, Villebon-sur-Yvette, France), purple crystal staining (Sigma, Darmstadt, Germany), FPR2 antagonists WRW4 (Alomone Labs, Jerusalem, Israel), monoclonal anti-FPR2 antibody (clone FN-1D6-AI, Genova, Italy), monoclonal anti-Nucleoprotein (NP, HB-65, invivomab), conjugated Alexa Fluor 488 anti-Rab5 antibody (Santa Cruz, Heidelberg, Germany), IgG control antibody (ThermoFisher, Villebon-sur-Yvette, France), ERK inhibitor pathway U0126 (Sigma-Aldrich, Darmstadt, Germany). ..

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  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Alomone Labs wrw4
    PSMα1-induced neutrophil necroptosis was blocked by <t>WRW4</t> or anti-TNFα. a, c Neutrophil were treated with PBS or TNFα neutralizing antibody at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6) and the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3). b, d, e Neutrophil were treated with PBS or WRW4 at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6), the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3), and the level of TNFα was detected by ELISA assay ( n = 8). Data were shown as mean ± SE (a, b, e). * P
    Wrw4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wrw4/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wrw4 - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    PSMα1-induced neutrophil necroptosis was blocked by WRW4 or anti-TNFα. a, c Neutrophil were treated with PBS or TNFα neutralizing antibody at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6) and the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3). b, d, e Neutrophil were treated with PBS or WRW4 at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6), the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3), and the level of TNFα was detected by ELISA assay ( n = 8). Data were shown as mean ± SE (a, b, e). * P

    Journal: Cell Death & Disease

    Article Title: Inhibiting PSMα-induced neutrophil necroptosis protects mice with MRSA pneumonia by blocking the agr system

    doi: 10.1038/s41419-018-0398-z

    Figure Lengend Snippet: PSMα1-induced neutrophil necroptosis was blocked by WRW4 or anti-TNFα. a, c Neutrophil were treated with PBS or TNFα neutralizing antibody at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6) and the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3). b, d, e Neutrophil were treated with PBS or WRW4 at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6), the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3), and the level of TNFα was detected by ELISA assay ( n = 8). Data were shown as mean ± SE (a, b, e). * P

    Article Snippet: Neutrophil cells were respectively stimulated with PSM peptide for 1 h at 37 °C and then centrifugated (1000 rpm, 10 min) after pretreated with or without NSA, Nec, WRW4, anti-TNFα for 1 h. The neutrophil cell culture supernatant is used to detect the release of LDH.

    Techniques: Incubation, Western Blot, Enzyme-linked Immunosorbent Assay