uricase enzyme  (Worthington Biochemical)


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  • 90
    Name:
    Uricase
    Description:
    A soluble lyophilized preparation
    Catalog Number:
    ls003855
    Price:
    Inquire
    Size:
    Bulk
    Source:
    Candida utilis
    Cas Number:
    9002.12.4
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    Structured Review

    Worthington Biochemical uricase enzyme
    Interactions between uric acid crystals and rabbit peripheral blood leukocytes (PBLs). Uric acid was again generated by addition of 400 μM hypoxanthine +0.5 U/mL XO to suspensions of freshly isolated PBLs for 1.5 h, followed by Cytospin centrifugation and staining. In some conditions (Panels B and C), 600 U/mL catalase was added to break down hydrogen peroxide, while in others 2 U/mL <t>uricase</t> was added to remove uric acid by conversion to allantoin. Panel A, large cluster of PBLs surrounding a uric acid crystal 85 μm in length; original magnification, 400×. Panels B and C, a pair of images showing a uric acid crystal (Panel B, yellow arrow) completely surrounded by a large aggregate of PBLs (Panel C), where the yellow arrow again indicates the location of the crystal, which appears as a non-descript piece of debris on brightfield examination. Panel D, addition of uricase abolished the formation of crystals and also the aggregates of PBLs. Panel E, heterophil adhering to a uric acid crystal and showing release of its secretory granules (green arrow). Panel F, PBLs adhering to a uric acid crystals show elongation (green arrows), and a mononuclear cell shows signs of apoptosis (red arrow); original magnification of Panels E and F, 600 X.
    A soluble lyophilized preparation
    https://www.bioz.com/result/uricase enzyme/product/Worthington Biochemical
    Average 90 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    uricase enzyme - by Bioz Stars, 2020-05
    90/100 stars

    Images

    1) Product Images from "Pro-inflammatory effects of uric acid in the gastrointestinal tract"

    Article Title: Pro-inflammatory effects of uric acid in the gastrointestinal tract

    Journal: Immunological investigations

    doi: 10.3109/08820139.2013.864667

    Interactions between uric acid crystals and rabbit peripheral blood leukocytes (PBLs). Uric acid was again generated by addition of 400 μM hypoxanthine +0.5 U/mL XO to suspensions of freshly isolated PBLs for 1.5 h, followed by Cytospin centrifugation and staining. In some conditions (Panels B and C), 600 U/mL catalase was added to break down hydrogen peroxide, while in others 2 U/mL uricase was added to remove uric acid by conversion to allantoin. Panel A, large cluster of PBLs surrounding a uric acid crystal 85 μm in length; original magnification, 400×. Panels B and C, a pair of images showing a uric acid crystal (Panel B, yellow arrow) completely surrounded by a large aggregate of PBLs (Panel C), where the yellow arrow again indicates the location of the crystal, which appears as a non-descript piece of debris on brightfield examination. Panel D, addition of uricase abolished the formation of crystals and also the aggregates of PBLs. Panel E, heterophil adhering to a uric acid crystal and showing release of its secretory granules (green arrow). Panel F, PBLs adhering to a uric acid crystals show elongation (green arrows), and a mononuclear cell shows signs of apoptosis (red arrow); original magnification of Panels E and F, 600 X.
    Figure Legend Snippet: Interactions between uric acid crystals and rabbit peripheral blood leukocytes (PBLs). Uric acid was again generated by addition of 400 μM hypoxanthine +0.5 U/mL XO to suspensions of freshly isolated PBLs for 1.5 h, followed by Cytospin centrifugation and staining. In some conditions (Panels B and C), 600 U/mL catalase was added to break down hydrogen peroxide, while in others 2 U/mL uricase was added to remove uric acid by conversion to allantoin. Panel A, large cluster of PBLs surrounding a uric acid crystal 85 μm in length; original magnification, 400×. Panels B and C, a pair of images showing a uric acid crystal (Panel B, yellow arrow) completely surrounded by a large aggregate of PBLs (Panel C), where the yellow arrow again indicates the location of the crystal, which appears as a non-descript piece of debris on brightfield examination. Panel D, addition of uricase abolished the formation of crystals and also the aggregates of PBLs. Panel E, heterophil adhering to a uric acid crystal and showing release of its secretory granules (green arrow). Panel F, PBLs adhering to a uric acid crystals show elongation (green arrows), and a mononuclear cell shows signs of apoptosis (red arrow); original magnification of Panels E and F, 600 X.

    Techniques Used: Generated, Isolation, Centrifugation, Staining

    Related Articles

    other:

    Article Title: Role of Host Xanthine Oxidase in Infection Due to Enteropathogenic and Shiga-Toxigenic Escherichia coli
    Article Snippet: Uricase was from Worthington Biochemicals (Freehold, NJ).

    Article Title: Biological Activities of Uric Acid in Infection Due to Enteropathogenic and Shiga-Toxigenic Escherichia coli
    Article Snippet: DNase I, micrococcal nuclease, and uricase were from Worthington Biochemicals, Lakewood, NJ.

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