westar nova 2 0 ecl solution  (Cyanagen)


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    Cyanagen westar nova 2 0 ecl solution
    Westar Nova 2 0 Ecl Solution, supplied by Cyanagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    westar nova 2 0 ecl solution  (Cyanagen)


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    Cyanagen westar nova 2 0 ecl solution
    Westar Nova 2 0 Ecl Solution, supplied by Cyanagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    chemiluminescence ecl detection kit westar nova 2 0  (Cyanagen)


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    Cyanagen chemiluminescence ecl detection kit westar nova 2 0
    Chemiluminescence Ecl Detection Kit Westar Nova 2 0, supplied by Cyanagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    chemiluminescence  (Cyanagen)


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    Cyanagen chemiluminescence
    Chemiluminescence, supplied by Cyanagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    chemiluminescence  (Cyanagen)


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    Cyanagen chemiluminescence
    Chemiluminescence, supplied by Cyanagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    westar nova 2 0 chemiluminescent substrate  (Cyanagen)


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    Cyanagen westar nova 2 0 chemiluminescent substrate
    Westar Nova 2 0 Chemiluminescent Substrate, supplied by Cyanagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    western blotting kit  (Cyanagen)


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    Cyanagen western blotting kit
    Western Blotting Kit, supplied by Cyanagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    westar nova 2 0 β actin  (Cyanagen)


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    Cyanagen westar nova 2 0 β actin
    Cisplatin, doxorubicin, PAC-1, and serum depletion up-regulate FGFR1 in MDCK cells. ( A ) Arithmetic mean ± SEM of FGFR1 mRNA levels relative to TBP in MDCK cells treated with or without 10 μM cisplatin for 24 h ( n = 5, paired t- test). ( B ) Left panel: Arithmetic mean ± SEM of FGFR1 protein abundance normalized to the abundance of <t>β-actin</t> in MDCK cells following treatment with or without 10 μM cisplatin for 24 h ( n = 7, one-sample t -test). Right panel: Original Western Blot demonstrating the abundance of FGFR1 in MDCK cells treated with (cis) or without (ctr) 10 μM cisplatin for 24 h. ( C ) Arithmetic mean ± SEM of FGFR1 transcript levels relative to TBP in MDCK cells treated with or without 300 nM doxorubicin for 24 h ( n = 4, paired t -test). ( D ) Arithmetic mean ± SEM of FGFR1 transcript level relative to TBP in MDCK cells treated with or without 10 μM PAC-1 for 24 h ( n = 5, paired t -test). ( E ) Arithmetic mean ± SEM of FGFR1 transcripts relative to TBP in MDCK cells incubated without or with 5 % FBS in culture medium for 24 h ( n = 5, paired t -test). * p < 0.05, ** p < 0.01 indicate significant difference from vehicle control; Abbreviations: a. u.: arbitrary units; cis cisplatin; ctr: control.
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    1) Product Images from "Impact of cytotoxic agents or apoptosis stimulants on αklotho in MDCK, NRK-52E and HK2 kidney cells"

    Article Title: Impact of cytotoxic agents or apoptosis stimulants on αklotho in MDCK, NRK-52E and HK2 kidney cells

    Journal: Aging (Albany NY)

    doi: 10.18632/aging.204238

    Cisplatin, doxorubicin, PAC-1, and serum depletion up-regulate FGFR1 in MDCK cells. ( A ) Arithmetic mean ± SEM of FGFR1 mRNA levels relative to TBP in MDCK cells treated with or without 10 μM cisplatin for 24 h ( n = 5, paired t- test). ( B ) Left panel: Arithmetic mean ± SEM of FGFR1 protein abundance normalized to the abundance of β-actin in MDCK cells following treatment with or without 10 μM cisplatin for 24 h ( n = 7, one-sample t -test). Right panel: Original Western Blot demonstrating the abundance of FGFR1 in MDCK cells treated with (cis) or without (ctr) 10 μM cisplatin for 24 h. ( C ) Arithmetic mean ± SEM of FGFR1 transcript levels relative to TBP in MDCK cells treated with or without 300 nM doxorubicin for 24 h ( n = 4, paired t -test). ( D ) Arithmetic mean ± SEM of FGFR1 transcript level relative to TBP in MDCK cells treated with or without 10 μM PAC-1 for 24 h ( n = 5, paired t -test). ( E ) Arithmetic mean ± SEM of FGFR1 transcripts relative to TBP in MDCK cells incubated without or with 5 % FBS in culture medium for 24 h ( n = 5, paired t -test). * p < 0.05, ** p < 0.01 indicate significant difference from vehicle control; Abbreviations: a. u.: arbitrary units; cis cisplatin; ctr: control.
    Figure Legend Snippet: Cisplatin, doxorubicin, PAC-1, and serum depletion up-regulate FGFR1 in MDCK cells. ( A ) Arithmetic mean ± SEM of FGFR1 mRNA levels relative to TBP in MDCK cells treated with or without 10 μM cisplatin for 24 h ( n = 5, paired t- test). ( B ) Left panel: Arithmetic mean ± SEM of FGFR1 protein abundance normalized to the abundance of β-actin in MDCK cells following treatment with or without 10 μM cisplatin for 24 h ( n = 7, one-sample t -test). Right panel: Original Western Blot demonstrating the abundance of FGFR1 in MDCK cells treated with (cis) or without (ctr) 10 μM cisplatin for 24 h. ( C ) Arithmetic mean ± SEM of FGFR1 transcript levels relative to TBP in MDCK cells treated with or without 300 nM doxorubicin for 24 h ( n = 4, paired t -test). ( D ) Arithmetic mean ± SEM of FGFR1 transcript level relative to TBP in MDCK cells treated with or without 10 μM PAC-1 for 24 h ( n = 5, paired t -test). ( E ) Arithmetic mean ± SEM of FGFR1 transcripts relative to TBP in MDCK cells incubated without or with 5 % FBS in culture medium for 24 h ( n = 5, paired t -test). * p < 0.05, ** p < 0.01 indicate significant difference from vehicle control; Abbreviations: a. u.: arbitrary units; cis cisplatin; ctr: control.

    Techniques Used: Serum Depletion, Western Blot, Incubation

    westar nova 2 0 chemiluminescent substrate  (Cyanagen)


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    Cyanagen westar nova 2 0 chemiluminescent substrate
    Westar Nova 2 0 Chemiluminescent Substrate, supplied by Cyanagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecl westar nova 2 0 detection system  (Cyanagen)


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    Cyanagen ecl westar nova 2 0 detection system
    Ecl Westar Nova 2 0 Detection System, supplied by Cyanagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    westar nova 2 0 chemiluminescent substrate  (Cyanagen)


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    Cyanagen westar nova 2 0 chemiluminescent substrate
    Westar Nova 2 0 Chemiluminescent Substrate, supplied by Cyanagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cyanagen westar nova 2 0 β actin
    Cisplatin, doxorubicin, PAC-1, and serum depletion up-regulate FGFR1 in MDCK cells. ( A ) Arithmetic mean ± SEM of FGFR1 mRNA levels relative to TBP in MDCK cells treated with or without 10 μM cisplatin for 24 h ( n = 5, paired t- test). ( B ) Left panel: Arithmetic mean ± SEM of FGFR1 protein abundance normalized to the abundance of <t>β-actin</t> in MDCK cells following treatment with or without 10 μM cisplatin for 24 h ( n = 7, one-sample t -test). Right panel: Original Western Blot demonstrating the abundance of FGFR1 in MDCK cells treated with (cis) or without (ctr) 10 μM cisplatin for 24 h. ( C ) Arithmetic mean ± SEM of FGFR1 transcript levels relative to TBP in MDCK cells treated with or without 300 nM doxorubicin for 24 h ( n = 4, paired t -test). ( D ) Arithmetic mean ± SEM of FGFR1 transcript level relative to TBP in MDCK cells treated with or without 10 μM PAC-1 for 24 h ( n = 5, paired t -test). ( E ) Arithmetic mean ± SEM of FGFR1 transcripts relative to TBP in MDCK cells incubated without or with 5 % FBS in culture medium for 24 h ( n = 5, paired t -test). * p < 0.05, ** p < 0.01 indicate significant difference from vehicle control; Abbreviations: a. u.: arbitrary units; cis cisplatin; ctr: control.
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    Cyanagen ecl westar nova 2 0 detection system
    Cisplatin, doxorubicin, PAC-1, and serum depletion up-regulate FGFR1 in MDCK cells. ( A ) Arithmetic mean ± SEM of FGFR1 mRNA levels relative to TBP in MDCK cells treated with or without 10 μM cisplatin for 24 h ( n = 5, paired t- test). ( B ) Left panel: Arithmetic mean ± SEM of FGFR1 protein abundance normalized to the abundance of <t>β-actin</t> in MDCK cells following treatment with or without 10 μM cisplatin for 24 h ( n = 7, one-sample t -test). Right panel: Original Western Blot demonstrating the abundance of FGFR1 in MDCK cells treated with (cis) or without (ctr) 10 μM cisplatin for 24 h. ( C ) Arithmetic mean ± SEM of FGFR1 transcript levels relative to TBP in MDCK cells treated with or without 300 nM doxorubicin for 24 h ( n = 4, paired t -test). ( D ) Arithmetic mean ± SEM of FGFR1 transcript level relative to TBP in MDCK cells treated with or without 10 μM PAC-1 for 24 h ( n = 5, paired t -test). ( E ) Arithmetic mean ± SEM of FGFR1 transcripts relative to TBP in MDCK cells incubated without or with 5 % FBS in culture medium for 24 h ( n = 5, paired t -test). * p < 0.05, ** p < 0.01 indicate significant difference from vehicle control; Abbreviations: a. u.: arbitrary units; cis cisplatin; ctr: control.
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    Image Search Results


    Cisplatin, doxorubicin, PAC-1, and serum depletion up-regulate FGFR1 in MDCK cells. ( A ) Arithmetic mean ± SEM of FGFR1 mRNA levels relative to TBP in MDCK cells treated with or without 10 μM cisplatin for 24 h ( n = 5, paired t- test). ( B ) Left panel: Arithmetic mean ± SEM of FGFR1 protein abundance normalized to the abundance of β-actin in MDCK cells following treatment with or without 10 μM cisplatin for 24 h ( n = 7, one-sample t -test). Right panel: Original Western Blot demonstrating the abundance of FGFR1 in MDCK cells treated with (cis) or without (ctr) 10 μM cisplatin for 24 h. ( C ) Arithmetic mean ± SEM of FGFR1 transcript levels relative to TBP in MDCK cells treated with or without 300 nM doxorubicin for 24 h ( n = 4, paired t -test). ( D ) Arithmetic mean ± SEM of FGFR1 transcript level relative to TBP in MDCK cells treated with or without 10 μM PAC-1 for 24 h ( n = 5, paired t -test). ( E ) Arithmetic mean ± SEM of FGFR1 transcripts relative to TBP in MDCK cells incubated without or with 5 % FBS in culture medium for 24 h ( n = 5, paired t -test). * p < 0.05, ** p < 0.01 indicate significant difference from vehicle control; Abbreviations: a. u.: arbitrary units; cis cisplatin; ctr: control.

    Journal: Aging (Albany NY)

    Article Title: Impact of cytotoxic agents or apoptosis stimulants on αklotho in MDCK, NRK-52E and HK2 kidney cells

    doi: 10.18632/aging.204238

    Figure Lengend Snippet: Cisplatin, doxorubicin, PAC-1, and serum depletion up-regulate FGFR1 in MDCK cells. ( A ) Arithmetic mean ± SEM of FGFR1 mRNA levels relative to TBP in MDCK cells treated with or without 10 μM cisplatin for 24 h ( n = 5, paired t- test). ( B ) Left panel: Arithmetic mean ± SEM of FGFR1 protein abundance normalized to the abundance of β-actin in MDCK cells following treatment with or without 10 μM cisplatin for 24 h ( n = 7, one-sample t -test). Right panel: Original Western Blot demonstrating the abundance of FGFR1 in MDCK cells treated with (cis) or without (ctr) 10 μM cisplatin for 24 h. ( C ) Arithmetic mean ± SEM of FGFR1 transcript levels relative to TBP in MDCK cells treated with or without 300 nM doxorubicin for 24 h ( n = 4, paired t -test). ( D ) Arithmetic mean ± SEM of FGFR1 transcript level relative to TBP in MDCK cells treated with or without 10 μM PAC-1 for 24 h ( n = 5, paired t -test). ( E ) Arithmetic mean ± SEM of FGFR1 transcripts relative to TBP in MDCK cells incubated without or with 5 % FBS in culture medium for 24 h ( n = 5, paired t -test). * p < 0.05, ** p < 0.01 indicate significant difference from vehicle control; Abbreviations: a. u.: arbitrary units; cis cisplatin; ctr: control.

    Article Snippet: For visualization, membranes were incubated for 2 min with Westar Nova 2.0 (β-actin) or Westar Supernova (FGFR1) ECL substrate (both from Cyanagen, Bologna, Italy).

    Techniques: Serum Depletion, Western Blot, Incubation