Article Title: Insulin potentiates the synchronous firing of arcuate nucleus Kiss1 neurons that protects against diet-induced obesity
Figure Lengend Snippet: Insulin augments the slow EPSP in Kiss1 ARH neurons from Kiss1 Cre ::Ai32 mice in a PI3K dependent manner and senktide-induced depolarization is antagonized by TRPC5 channel blockers. A–D , representative traces of the slow EPSPs in the presence or absence of PI3 kinase inhibitor wortmannin (100 nM) or insulin (20 nM). E , summary of the effects of wortmannin on the insulin-potentiated slow EPSP. The PI3 kinase blocker was applied for 15 min before the application of insulin (20 nM). Comparisons between different treatments were assessed using a one-way ANOVA (F (3, 47) = 5.0301, p
Article Snippet: Thapsigargin (Tg, 2 mM), TacR3 agonist senktide (1 mM), TRPC4/5 antagonist, HC 070 (from MCE, 10 mM), and the PI3K inhibitor wortmannin (Alomone Labs; 100 μM) were prepared in dimethylsulfoxide (DMSO).
Techniques: Mouse Assay