hcov 229e alpha coronavirus vr 740  (ATCC)


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    ATCC hcov 229e alpha coronavirus vr 740
    Hcov 229e Alpha Coronavirus Vr 740, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcov 229e vr 740 virus strain  (ATCC)


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    ATCC hcov 229e vr 740 virus strain
    Hcov 229e Vr 740 Virus Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcov 229e vr 740 strains  (ATCC)


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    ATCC hcov 229e vr 740 strains
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    hcov 229e strain vr 740  (ATCC)


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    ATCC hcov 229e strain vr 740
    Hcov 229e Strain Vr 740, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcov 229e strain vr 740  (ATCC)


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    ATCC hcov 229e strain vr 740
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    human coronavirus 229e vr 740  (ATCC)


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    ATCC human coronavirus 229e vr 740
    a, Phylogenetic tree based on spike protein amino acid sequences of representative coronaviruses. b, Workflow demonstrating the customization of nanobody-based CVRs for specific coronaviruses. c, Binding kinetics between optimal nanobodies and their respective antigens. d, Coronavirus CTD or S1 binding in HEK293T cells transiently expressing the corresponding CVRs. Dashed lines indicate thresholds for positive ratio calculation. e, PSV entry efficiencies of 12 representative coronaviruses in HEK293T cells transiently expressing the indicated CVRs. f, <t>229E</t> S1-mFc binding, fusion, and authentic infection in HEK293T stably expressing APN or 4H5-CVR. g-i, Evaluation of NTD-mediated (g) or CTD-mediated (h) MHV antigen binding, cell-cell fusion, and authentic MHV infection in HEK293T cells stably expressing mCEACAM1a or the indicated CVRs. MHV-A59 RNA copies (N gene) in supernatant was quantified for infected cells expressing mCEACAM1a or 1B3-CVR ( i ). j, Summary of the IC 50 of several broadly neutralizing antibodies against PSV entry of representative coronaviruses in HEK293T stably expressing the corresponding CVRs. The RBD-targeting REGN 10933 (REGN) was employed as a control. / : no inhibition detected. k, Inhibitory efficacy of inhibitors against PSV entry of SARS-CoV-2, HKU1, HKU3 and HKU5 in HEK293T cells stably expressing the indicated CVRs. Infection was examined by S protein immunofluorescence at 24 hpi for f , g , and h . Scale bars: 100 μm. Data are presented as mean ± SD and n=2-3 biologically repeats for d, e, i, and k . One-way ANOVA analysis followed by Dunnett’s test for k; unpaired two-tailed Student’s t-tests for e .
    Human Coronavirus 229e Vr 740, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Engineering customized viral receptors for various coronaviruses"

    Article Title: Engineering customized viral receptors for various coronaviruses

    Journal: bioRxiv

    doi: 10.1101/2024.03.03.583237

    a, Phylogenetic tree based on spike protein amino acid sequences of representative coronaviruses. b, Workflow demonstrating the customization of nanobody-based CVRs for specific coronaviruses. c, Binding kinetics between optimal nanobodies and their respective antigens. d, Coronavirus CTD or S1 binding in HEK293T cells transiently expressing the corresponding CVRs. Dashed lines indicate thresholds for positive ratio calculation. e, PSV entry efficiencies of 12 representative coronaviruses in HEK293T cells transiently expressing the indicated CVRs. f, 229E S1-mFc binding, fusion, and authentic infection in HEK293T stably expressing APN or 4H5-CVR. g-i, Evaluation of NTD-mediated (g) or CTD-mediated (h) MHV antigen binding, cell-cell fusion, and authentic MHV infection in HEK293T cells stably expressing mCEACAM1a or the indicated CVRs. MHV-A59 RNA copies (N gene) in supernatant was quantified for infected cells expressing mCEACAM1a or 1B3-CVR ( i ). j, Summary of the IC 50 of several broadly neutralizing antibodies against PSV entry of representative coronaviruses in HEK293T stably expressing the corresponding CVRs. The RBD-targeting REGN 10933 (REGN) was employed as a control. / : no inhibition detected. k, Inhibitory efficacy of inhibitors against PSV entry of SARS-CoV-2, HKU1, HKU3 and HKU5 in HEK293T cells stably expressing the indicated CVRs. Infection was examined by S protein immunofluorescence at 24 hpi for f , g , and h . Scale bars: 100 μm. Data are presented as mean ± SD and n=2-3 biologically repeats for d, e, i, and k . One-way ANOVA analysis followed by Dunnett’s test for k; unpaired two-tailed Student’s t-tests for e .
    Figure Legend Snippet: a, Phylogenetic tree based on spike protein amino acid sequences of representative coronaviruses. b, Workflow demonstrating the customization of nanobody-based CVRs for specific coronaviruses. c, Binding kinetics between optimal nanobodies and their respective antigens. d, Coronavirus CTD or S1 binding in HEK293T cells transiently expressing the corresponding CVRs. Dashed lines indicate thresholds for positive ratio calculation. e, PSV entry efficiencies of 12 representative coronaviruses in HEK293T cells transiently expressing the indicated CVRs. f, 229E S1-mFc binding, fusion, and authentic infection in HEK293T stably expressing APN or 4H5-CVR. g-i, Evaluation of NTD-mediated (g) or CTD-mediated (h) MHV antigen binding, cell-cell fusion, and authentic MHV infection in HEK293T cells stably expressing mCEACAM1a or the indicated CVRs. MHV-A59 RNA copies (N gene) in supernatant was quantified for infected cells expressing mCEACAM1a or 1B3-CVR ( i ). j, Summary of the IC 50 of several broadly neutralizing antibodies against PSV entry of representative coronaviruses in HEK293T stably expressing the corresponding CVRs. The RBD-targeting REGN 10933 (REGN) was employed as a control. / : no inhibition detected. k, Inhibitory efficacy of inhibitors against PSV entry of SARS-CoV-2, HKU1, HKU3 and HKU5 in HEK293T cells stably expressing the indicated CVRs. Infection was examined by S protein immunofluorescence at 24 hpi for f , g , and h . Scale bars: 100 μm. Data are presented as mean ± SD and n=2-3 biologically repeats for d, e, i, and k . One-way ANOVA analysis followed by Dunnett’s test for k; unpaired two-tailed Student’s t-tests for e .

    Techniques Used: Binding Assay, Expressing, Infection, Stable Transfection, Inhibition, Immunofluorescence, Two Tailed Test

    vr 740  (ATCC)


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    ATCC vr 740
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    vr 740  (ATCC)


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    n a human coronavirus 229e atcc vr 740 betacov hong kong vm20001061 2020 lv  (ATCC)


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    ATCC n a human coronavirus 229e atcc vr 740 betacov hong kong vm20001061 2020 lv
    N A Human Coronavirus 229e Atcc Vr 740 Betacov Hong Kong Vm20001061 2020 Lv, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcov 229e strain vr 740  (ATCC)


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    ATCC hcov 229e strain vr 740
    Screening of the antiviral activity of crude methanolic extracts <t>on</t> <t>HCoV-229E-Luc.</t> Huh-7 or Huh-7/TMPRSS2 cells were inoculated with HCoV-229E-Luc in the presence of various plant extracts at 25 µg/mL. Cells were lysed 7 h post-inoculation, and luciferase activity was quantified. Experiments were performed in triplicate, with each experiment being repeated thrice. The data bars represent the mean ± SEM. The asterisk indicates a statistical difference compared to the control (*, p < 0.05; **, p < 0.01).
    Hcov 229e Strain Vr 740, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Discovery of Anti-Coronavirus Cinnamoyl Triterpenoids Isolated from Hippophae rhamnoides during a Screening of Halophytes from the North Sea and Channel Coasts in Northern France"

    Article Title: Discovery of Anti-Coronavirus Cinnamoyl Triterpenoids Isolated from Hippophae rhamnoides during a Screening of Halophytes from the North Sea and Channel Coasts in Northern France

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms242316617

    Screening of the antiviral activity of crude methanolic extracts on HCoV-229E-Luc. Huh-7 or Huh-7/TMPRSS2 cells were inoculated with HCoV-229E-Luc in the presence of various plant extracts at 25 µg/mL. Cells were lysed 7 h post-inoculation, and luciferase activity was quantified. Experiments were performed in triplicate, with each experiment being repeated thrice. The data bars represent the mean ± SEM. The asterisk indicates a statistical difference compared to the control (*, p < 0.05; **, p < 0.01).
    Figure Legend Snippet: Screening of the antiviral activity of crude methanolic extracts on HCoV-229E-Luc. Huh-7 or Huh-7/TMPRSS2 cells were inoculated with HCoV-229E-Luc in the presence of various plant extracts at 25 µg/mL. Cells were lysed 7 h post-inoculation, and luciferase activity was quantified. Experiments were performed in triplicate, with each experiment being repeated thrice. The data bars represent the mean ± SEM. The asterisk indicates a statistical difference compared to the control (*, p < 0.05; **, p < 0.01).

    Techniques Used: Activity Assay, Luciferase

    Cytotoxicity, antiviral activity, and SI of each of the crude methanolic extracts against  HCoV-229E.
    Figure Legend Snippet: Cytotoxicity, antiviral activity, and SI of each of the crude methanolic extracts against HCoV-229E.

    Techniques Used: Activity Assay

    Inhibitory activity of the three partitions obtained from each plant’s crude methanolic extract on HCoV-229E-Luc infection. Experiments were conducted as described earlier. Data are represented as the mean ± SEM of three independent experiments. (*, p < 0.05; **, p < 0.01; ***, p < 0.001).
    Figure Legend Snippet: Inhibitory activity of the three partitions obtained from each plant’s crude methanolic extract on HCoV-229E-Luc infection. Experiments were conducted as described earlier. Data are represented as the mean ± SEM of three independent experiments. (*, p < 0.05; **, p < 0.01; ***, p < 0.001).

    Techniques Used: Activity Assay, Infection

    Cytotoxicity, activity, and SI of each of the sub-extracts against  HCoV-229E.
    Figure Legend Snippet: Cytotoxicity, activity, and SI of each of the sub-extracts against HCoV-229E.

    Techniques Used: Activity Assay

    Antiviral activity of Hippophae rhamnoides fractions. ( A ) Screening of the antiviral activity of the fractions of Hippophae rhamnoides with HCoV-229E-Luc. The different fractions were tested at 25 μg/mL. ( B ) Western blotting analysis showing the effects of the different fractions of Hippophae rhamnoides on N protein expression in Vero-81 cells. Vero-81 cells were infected with SARS-CoV-2 in the presence of the different fractions at 10 and 25 µg/mL. Cell lysates were collected after 16 h and subjected to Western blotting. (***, p < 0.001).
    Figure Legend Snippet: Antiviral activity of Hippophae rhamnoides fractions. ( A ) Screening of the antiviral activity of the fractions of Hippophae rhamnoides with HCoV-229E-Luc. The different fractions were tested at 25 μg/mL. ( B ) Western blotting analysis showing the effects of the different fractions of Hippophae rhamnoides on N protein expression in Vero-81 cells. Vero-81 cells were infected with SARS-CoV-2 in the presence of the different fractions at 10 and 25 µg/mL. Cell lysates were collected after 16 h and subjected to Western blotting. (***, p < 0.001).

    Techniques Used: Activity Assay, Western Blot, Expressing, Infection

    Cytotoxicity, antiviral activity, and SI of each purified compound against  HCoV-229E.
    Figure Legend Snippet: Cytotoxicity, antiviral activity, and SI of each purified compound against HCoV-229E.

    Techniques Used: Activity Assay, Purification

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    ATCC hcov 229e alpha coronavirus vr 740
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    ATCC human coronavirus 229e vr 740
    a, Phylogenetic tree based on spike protein amino acid sequences of representative coronaviruses. b, Workflow demonstrating the customization of nanobody-based CVRs for specific coronaviruses. c, Binding kinetics between optimal nanobodies and their respective antigens. d, Coronavirus CTD or S1 binding in HEK293T cells transiently expressing the corresponding CVRs. Dashed lines indicate thresholds for positive ratio calculation. e, PSV entry efficiencies of 12 representative coronaviruses in HEK293T cells transiently expressing the indicated CVRs. f, <t>229E</t> S1-mFc binding, fusion, and authentic infection in HEK293T stably expressing APN or 4H5-CVR. g-i, Evaluation of NTD-mediated (g) or CTD-mediated (h) MHV antigen binding, cell-cell fusion, and authentic MHV infection in HEK293T cells stably expressing mCEACAM1a or the indicated CVRs. MHV-A59 RNA copies (N gene) in supernatant was quantified for infected cells expressing mCEACAM1a or 1B3-CVR ( i ). j, Summary of the IC 50 of several broadly neutralizing antibodies against PSV entry of representative coronaviruses in HEK293T stably expressing the corresponding CVRs. The RBD-targeting REGN 10933 (REGN) was employed as a control. / : no inhibition detected. k, Inhibitory efficacy of inhibitors against PSV entry of SARS-CoV-2, HKU1, HKU3 and HKU5 in HEK293T cells stably expressing the indicated CVRs. Infection was examined by S protein immunofluorescence at 24 hpi for f , g , and h . Scale bars: 100 μm. Data are presented as mean ± SD and n=2-3 biologically repeats for d, e, i, and k . One-way ANOVA analysis followed by Dunnett’s test for k; unpaired two-tailed Student’s t-tests for e .
    Human Coronavirus 229e Vr 740, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vr 740  (ATCC)
    86
    ATCC vr 740
    a, Phylogenetic tree based on spike protein amino acid sequences of representative coronaviruses. b, Workflow demonstrating the customization of nanobody-based CVRs for specific coronaviruses. c, Binding kinetics between optimal nanobodies and their respective antigens. d, Coronavirus CTD or S1 binding in HEK293T cells transiently expressing the corresponding CVRs. Dashed lines indicate thresholds for positive ratio calculation. e, PSV entry efficiencies of 12 representative coronaviruses in HEK293T cells transiently expressing the indicated CVRs. f, <t>229E</t> S1-mFc binding, fusion, and authentic infection in HEK293T stably expressing APN or 4H5-CVR. g-i, Evaluation of NTD-mediated (g) or CTD-mediated (h) MHV antigen binding, cell-cell fusion, and authentic MHV infection in HEK293T cells stably expressing mCEACAM1a or the indicated CVRs. MHV-A59 RNA copies (N gene) in supernatant was quantified for infected cells expressing mCEACAM1a or 1B3-CVR ( i ). j, Summary of the IC 50 of several broadly neutralizing antibodies against PSV entry of representative coronaviruses in HEK293T stably expressing the corresponding CVRs. The RBD-targeting REGN 10933 (REGN) was employed as a control. / : no inhibition detected. k, Inhibitory efficacy of inhibitors against PSV entry of SARS-CoV-2, HKU1, HKU3 and HKU5 in HEK293T cells stably expressing the indicated CVRs. Infection was examined by S protein immunofluorescence at 24 hpi for f , g , and h . Scale bars: 100 μm. Data are presented as mean ± SD and n=2-3 biologically repeats for d, e, i, and k . One-way ANOVA analysis followed by Dunnett’s test for k; unpaired two-tailed Student’s t-tests for e .
    Vr 740, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC n a human coronavirus 229e atcc vr 740 betacov hong kong vm20001061 2020 lv
    a, Phylogenetic tree based on spike protein amino acid sequences of representative coronaviruses. b, Workflow demonstrating the customization of nanobody-based CVRs for specific coronaviruses. c, Binding kinetics between optimal nanobodies and their respective antigens. d, Coronavirus CTD or S1 binding in HEK293T cells transiently expressing the corresponding CVRs. Dashed lines indicate thresholds for positive ratio calculation. e, PSV entry efficiencies of 12 representative coronaviruses in HEK293T cells transiently expressing the indicated CVRs. f, <t>229E</t> S1-mFc binding, fusion, and authentic infection in HEK293T stably expressing APN or 4H5-CVR. g-i, Evaluation of NTD-mediated (g) or CTD-mediated (h) MHV antigen binding, cell-cell fusion, and authentic MHV infection in HEK293T cells stably expressing mCEACAM1a or the indicated CVRs. MHV-A59 RNA copies (N gene) in supernatant was quantified for infected cells expressing mCEACAM1a or 1B3-CVR ( i ). j, Summary of the IC 50 of several broadly neutralizing antibodies against PSV entry of representative coronaviruses in HEK293T stably expressing the corresponding CVRs. The RBD-targeting REGN 10933 (REGN) was employed as a control. / : no inhibition detected. k, Inhibitory efficacy of inhibitors against PSV entry of SARS-CoV-2, HKU1, HKU3 and HKU5 in HEK293T cells stably expressing the indicated CVRs. Infection was examined by S protein immunofluorescence at 24 hpi for f , g , and h . Scale bars: 100 μm. Data are presented as mean ± SD and n=2-3 biologically repeats for d, e, i, and k . One-way ANOVA analysis followed by Dunnett’s test for k; unpaired two-tailed Student’s t-tests for e .
    N A Human Coronavirus 229e Atcc Vr 740 Betacov Hong Kong Vm20001061 2020 Lv, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a, Phylogenetic tree based on spike protein amino acid sequences of representative coronaviruses. b, Workflow demonstrating the customization of nanobody-based CVRs for specific coronaviruses. c, Binding kinetics between optimal nanobodies and their respective antigens. d, Coronavirus CTD or S1 binding in HEK293T cells transiently expressing the corresponding CVRs. Dashed lines indicate thresholds for positive ratio calculation. e, PSV entry efficiencies of 12 representative coronaviruses in HEK293T cells transiently expressing the indicated CVRs. f, 229E S1-mFc binding, fusion, and authentic infection in HEK293T stably expressing APN or 4H5-CVR. g-i, Evaluation of NTD-mediated (g) or CTD-mediated (h) MHV antigen binding, cell-cell fusion, and authentic MHV infection in HEK293T cells stably expressing mCEACAM1a or the indicated CVRs. MHV-A59 RNA copies (N gene) in supernatant was quantified for infected cells expressing mCEACAM1a or 1B3-CVR ( i ). j, Summary of the IC 50 of several broadly neutralizing antibodies against PSV entry of representative coronaviruses in HEK293T stably expressing the corresponding CVRs. The RBD-targeting REGN 10933 (REGN) was employed as a control. / : no inhibition detected. k, Inhibitory efficacy of inhibitors against PSV entry of SARS-CoV-2, HKU1, HKU3 and HKU5 in HEK293T cells stably expressing the indicated CVRs. Infection was examined by S protein immunofluorescence at 24 hpi for f , g , and h . Scale bars: 100 μm. Data are presented as mean ± SD and n=2-3 biologically repeats for d, e, i, and k . One-way ANOVA analysis followed by Dunnett’s test for k; unpaired two-tailed Student’s t-tests for e .

    Journal: bioRxiv

    Article Title: Engineering customized viral receptors for various coronaviruses

    doi: 10.1101/2024.03.03.583237

    Figure Lengend Snippet: a, Phylogenetic tree based on spike protein amino acid sequences of representative coronaviruses. b, Workflow demonstrating the customization of nanobody-based CVRs for specific coronaviruses. c, Binding kinetics between optimal nanobodies and their respective antigens. d, Coronavirus CTD or S1 binding in HEK293T cells transiently expressing the corresponding CVRs. Dashed lines indicate thresholds for positive ratio calculation. e, PSV entry efficiencies of 12 representative coronaviruses in HEK293T cells transiently expressing the indicated CVRs. f, 229E S1-mFc binding, fusion, and authentic infection in HEK293T stably expressing APN or 4H5-CVR. g-i, Evaluation of NTD-mediated (g) or CTD-mediated (h) MHV antigen binding, cell-cell fusion, and authentic MHV infection in HEK293T cells stably expressing mCEACAM1a or the indicated CVRs. MHV-A59 RNA copies (N gene) in supernatant was quantified for infected cells expressing mCEACAM1a or 1B3-CVR ( i ). j, Summary of the IC 50 of several broadly neutralizing antibodies against PSV entry of representative coronaviruses in HEK293T stably expressing the corresponding CVRs. The RBD-targeting REGN 10933 (REGN) was employed as a control. / : no inhibition detected. k, Inhibitory efficacy of inhibitors against PSV entry of SARS-CoV-2, HKU1, HKU3 and HKU5 in HEK293T cells stably expressing the indicated CVRs. Infection was examined by S protein immunofluorescence at 24 hpi for f , g , and h . Scale bars: 100 μm. Data are presented as mean ± SD and n=2-3 biologically repeats for d, e, i, and k . One-way ANOVA analysis followed by Dunnett’s test for k; unpaired two-tailed Student’s t-tests for e .

    Article Snippet: Human coronavirus 229E (VR-740) is obtained from ATCC and amplified in Huh-7 cells.

    Techniques: Binding Assay, Expressing, Infection, Stable Transfection, Inhibition, Immunofluorescence, Two Tailed Test