Journal: Virology Journal

Article Title: Evaluation of three Polymerase chain reaction tests targeting morphological transforming region II, UL-83 gene and glycoprotein O gene for the detection of Human Cytomegalovirus genome in clinical specimens of immunocompromised patients in Chennai, India

doi: 10.1186/1743-422X-3-20

Figure Lengend Snippet: Results of the three PCRs for the diagnosis of suspected CMV infections in comparison to pp65 antigenemia (gold standard) a .

Article Snippet: The PCRs for the aforementioned regions were already standardized in our laboratory using cultures of CMV AD-169 strain (ATCC VR-538). pp65 antigenemia assay is a rapid, reliable and superior to both rapid shell vial assay and conventional test tube culture in the detection of HCMV in the clinical specimens from immunocompromised patients indicating active HCMV disease.

Techniques:

Journal: Bioinformation

Article Title: Functional characterization of novel mutations in UL54 of ganciclovir resistant HCMV strain using structural analysis

doi:

Figure Lengend Snippet: The amplified products of region IV of the specimens along with others specimens detected positive to HCMV. Agarose Gel Electrophoretogram of PCR amplified product targeting Human cytomegalovirus UL54 gene of Region IV. Round II applied on Clinical Specimens. Some of the specimens positive for the region IV along with sample 2256 is shown. Lanes NC2: Negative Control - II round NC1: Negative Control - I round PC: Positive Control DNA extract from Human cytomegalovirus AD 169 MWM: Molecular weight marker 100 bp Ladder. Specimens positive for UL54 partial region: 2526, 4474, 4869, 1650, 4069

Article Snippet: DNA extracted from HCMV AD 169 (ATCC VR 538) was used as a positive control.

Techniques: Amplification, Agarose Gel Electrophoresis, Negative Control, Positive Control, Molecular Weight, Marker

Journal: International Journal of Bacteriology

Article Title: Rapid PCR Detection of Mycoplasma hominis , Ureaplasma urealyticum , and Ureaplasma parvum

doi: 10.1155/2013/168742

Figure Lengend Snippet: Cross-reactivity and inclusivity panel. Only Mycoplasma hominis, Ureaplasma parvum , and Ureaplasma urealyticum were detected (by the appropriate assays).

Article Snippet: Mycoplasma lipophilum , ATCC 27104 , Cytomegalovirus , ATCC VR-538.

Techniques:

Journal: Viruses

Article Title: Role of PARP-1 in Human Cytomegalovirus Infection and Functional Partners Encoded by This Virus

doi: 10.3390/v14092049

Figure Lengend Snippet: HCMV UL76 binds to the BRCT domain of PARP-1 through its C terminal. ( A ) NAD + levels declined to varying degrees upon HSV-1 and HCMV infection. Fibroblasts were infected with HSV-1 (F strain) or HCMV (AD169 strain) at an MOI of 1. Cells were collected at 24 hpi by trypsin digestion and lysed in a NAD + extraction buffer. NAD + levels in these cells were normalized to total protein and compared to mock-infected cells. Values are averages of triplicate experiments (±1 SD), * p < 0.05. ( B ) Protein PARylation and the protein levels of PARP-1 and PARG were compared between HSV-1- and HCMV-infected cells. MRC-5 cells were infected with HSV-1 or HCMV at an MOI of 1. Pp65 of HCMV and glycoprotein C of HSV-1 served as infection markers for each virus. The whole cell lysates were analyzed by indicated antibodies. ( C ) HCMV tegument protein UL76 was a PARP-1 binding protein. MRC-5 cells were infected or mock infected with HCMV at an MOI of 1 for 96 h. Cells were harvested and lysed in NETN 420 in the presence of protease inhibitors. Cell lysates were prepared and subjected to immunoprecipitation with PARP-1 antibody or control IgG. The presence of PARP-1 and UL76 in the immune complexes was determined by Western blot analysis. * indicates nonspecific band. ( D ) UL76 directly interacts with PARP-1 in vitro. Purified His-PARP-1 was incubated with equal amount of GST or GST-UL76. Samples were subjected to GST pull-down, and bound complexes were analyzed by SDS-PAGE. ( E ) Schematic depiction of PARP-1 and its deletion mutants. Results from domain mapping experiments indicated that UL76 binds to the BRCT domain of PARP-1. ( F ) BRCT domain of PARP-1 was responsible for the association between UL76 and PARP-1. SFB vector or SFP-UL76 was co-transfected with GFP-tagged PARP-1 WT or each deletion mutant. UL76 did not bind with the PARP-1 BRCT domain deletion mutant. ( G ) Schematic depiction of HCMV UL76. Results from domain mapping experiments indicated that PARP-1 binds to the C terminal of UL76. ( H ) HEK293T cells were transfected with a construct for SFB vector, SFB-tagged UL76, UL76 truncates or UL76 homologs in HSV-1 and KSHV, respectively. Cell lysates were subjected to SFB immunoprecipitation, and the presence of PARP-1 was analyzed by immunoblotting.

Article Snippet: HCMV AD169 strain (ATCC-VR538) was propagated in MRC-5 cells.

Techniques: Infection, Binding Assay, Immunoprecipitation, Western Blot, In Vitro, Purification, Incubation, SDS Page, Plasmid Preparation, Transfection, Mutagenesis, Construct

Journal: iScience

Article Title: Cytomegalovirus-specific neutralizing antibodies effectively prevent uncontrolled infection after allogeneic hematopoietic stem cell transplantation

doi: 10.1016/j.isci.2022.105065

Figure Lengend Snippet:

Article Snippet: HCMV-AD169 , ATCC , ATCC VR-538.

Techniques: Recombinant, Modification, Enzyme-linked Immunosorbent Assay, Staining, Software