Journal: Infectious Agents and Cancer

Article Title: Is human cytomegalovirus associated with breast cancer progression?

doi: 10.1186/1750-9378-8-12

Figure Lengend Snippet: Quality control for breast carcinomas and fibroadenomas. Multiplex PCR for GADPH. ( A ) Lines 1−9 and 19−26 breast carcinomas; 10−18 fibroadenomas. ( B ) Lines 27, 37−45 breast carcinomas; 19−30, 46 and 47 fibroadenomas. Base pairs (bp); control positive (+), HCMV strain AD-169 (ATCC® VR-583); control negative (−), DNA white blood cells from healthy individual (blood donor) and reaction mix without DNA.

Article Snippet: Positive control was done with HCMV strain AD-169 (ATCC VR-583).

Techniques: Multiplex Assay

Journal: NPJ Vaccines

Article Title: Measles-based Zika vaccine induces long-term immunity and requires NS1 antibodies to protect the female reproductive tract

doi: 10.1038/s41541-022-00464-2

Figure Lengend Snippet: a Timeline of vaccination, bone-marrow and spleen harvesting. ZIKV-E specific ( b ), ZIKV-NS1 specific ( c ), and MV-H specific ( d ) Plasma cells in the bone marrow and spleen. The mean of triplicates is depicted for each animal. Mean ± SD is depicted per group. e ZIKV Neutralization with PRVABC59 Asian strain. FRNT was performed on necropsy sera from vaccinated animals and controls. The mean 50% neutralizing titer of triplicate values is depicted per animal as IU/mL as ZIKV standards (Bei) were used. The Mean ± SD is depicted per group. f MV Neutralization with low-passage MV Edmonston strain. Nt-EIA assay was performed on necropsy sera from vaccinated animals and controls. The mean of 50% neutralizing titer (Nt-EIA titers) of triplicates is plotted per animal on the graph in terms of mIU/mL as bei standards were used. The Mean ± SD is depicted per group. Statistics for Fig. 5b–f were done using the one-way ANOVA with post hoc Tukey HSD test and performed on log-transformed data for each time point. P value of 0.1234(ns), 0.0332(*), 0.0021(**), 0.0002(***), <0.0001(****) are depicted accordingly. A horizontal line (—) is used to include all groups below it.

Article Snippet: Zika virus African MR766 strain (ATCC, VR-1838), Zika virus Asian PRVABC59 strain (ATCC, VR-1843), and Measles virus low-passage Edmonston strain (ATCC, VR-24).

Techniques: Neutralization, Enzyme Immunoassay, Transformation Assay

Journal: NPJ Vaccines

Article Title: Measles-based Zika vaccine induces long-term immunity and requires NS1 antibodies to protect the female reproductive tract

doi: 10.1038/s41541-022-00464-2

Figure Lengend Snippet: a Timeline of vaccination, bone-marrow and spleen harvesting. ZIKV-E specific ( b ), ZIKV-NS1 specific ( c ), and MV-H specific ( d ) Plasma cells in the bone marrow and spleen. The mean of triplicates is depicted for each animal. Mean ± SD is depicted per group. e ZIKV Neutralization with PRVABC59 Asian strain. FRNT was performed on necropsy sera from vaccinated animals and controls. The mean 50% neutralizing titer of triplicate values is depicted per animal as IU/mL as ZIKV standards (Bei) were used. The Mean ± SD is depicted per group. f MV Neutralization with low-passage MV Edmonston strain. Nt-EIA assay was performed on necropsy sera from vaccinated animals and controls. The mean of 50% neutralizing titer (Nt-EIA titers) of triplicates is plotted per animal on the graph in terms of mIU/mL as bei standards were used. The Mean ± SD is depicted per group. Statistics for Fig. 5b–f were done using the one-way ANOVA with post hoc Tukey HSD test and performed on log-transformed data for each time point. P value of 0.1234(ns), 0.0332(*), 0.0021(**), 0.0002(***), <0.0001(****) are depicted accordingly. A horizontal line (—) is used to include all groups below it.

Article Snippet: The low passage MV Edmonston strain (ATCC-VR 24) was propagated by inoculating 100 μL of the original ATCC vial into a T175 flask pre-seeded with Vero cells (80% confluent) in 32 mL of 1X DMEM (2% FBS) and placed 34 °C in a humidified 5% CO 2 atmosphere.

Techniques: Neutralization, Enzyme Immunoassay, Transformation Assay