copy number  (ATCC)

Journal: Virus Research

Article Title: Mechanisms of antiviral action and toxicities of ipecac alkaloids: Emetine and dehydroemetine exhibit anti-coronaviral activities at non-cardiotoxic concentrations

doi: 10.1016/j.virusres.2024.199322

Figure Lengend Snippet: Antiviral activity and selectivity of ipecac alkaloids toward HCoV-OC43 virus in Vero E6 cells. A – Emetine, or an analog, was introduced after absorption of HCoV-OC43 (MOI 20). Viral RNA in the medium 72 h following infection was isolated and viral growth was evaluated by RT-qPCR for the N-gene. B - Non-infected Vero E6 cells were exposed to ipecac alkaloids for 72 h followed by MTS assay to evaluate growth inhibition and toxicity in response to drugs. Experimental details are described in Materials and Methods. Filled circles – emetine; empty circles – DHE4; filled squares – DHE2; empty squares – isoemetine; filled diamonds – DHE1; empty diamonds – DHE3.

Article Snippet: Viral genome copy number was determined by reverse transcription coupled with quantitative PCR (RT-qPCR) using Quantitative Genomic RNA from Betacoronavirus 1 Strain OC43 (ATCC; VR-1558DQ) as a standard.

Techniques: Activity Assay, Virus, Infection, Isolation, Quantitative RT-PCR, MTS Assay, Inhibition

Journal: Virus Research

Article Title: Mechanisms of antiviral action and toxicities of ipecac alkaloids: Emetine and dehydroemetine exhibit anti-coronaviral activities at non-cardiotoxic concentrations

doi: 10.1016/j.virusres.2024.199322

Figure Lengend Snippet: Antiviral activity of selected ipecac alkaloids against SARS-CoV-2 virus in BEC-hACE2 cells. BEC-hACE2 cells were infected with SARS-CoV-2 (0.01 pfu per cell) for 2 h followed by incubation with emetine (filled circles), DHE4 (empty circles) or isoemetine (empty squares) for 24 hpi. Viral RNA from media was isolated and viral propagation was evaluated by RT-qPCR. Experimental details are described in Materials and Methods.

Article Snippet: Viral genome copy number was determined by reverse transcription coupled with quantitative PCR (RT-qPCR) using Quantitative Genomic RNA from Betacoronavirus 1 Strain OC43 (ATCC; VR-1558DQ) as a standard.

Techniques: Activity Assay, Virus, Infection, Incubation, Isolation, Quantitative RT-PCR

Journal: Virus Research

Article Title: Mechanisms of antiviral action and toxicities of ipecac alkaloids: Emetine and dehydroemetine exhibit anti-coronaviral activities at non-cardiotoxic concentrations

doi: 10.1016/j.virusres.2024.199322

Figure Lengend Snippet: Antiviral activity and selectivity of ipecac alkaloids toward HCoV-OC43 virus in Vero E6 cells. A – Emetine, or an analog, was introduced after absorption of HCoV-OC43 (MOI 20). Viral RNA in the medium 72 h following infection was isolated and viral growth was evaluated by RT-qPCR for the N-gene. B - Non-infected Vero E6 cells were exposed to ipecac alkaloids for 72 h followed by MTS assay to evaluate growth inhibition and toxicity in response to drugs. Experimental details are described in Materials and Methods. Filled circles – emetine; empty circles – DHE4; filled squares – DHE2; empty squares – isoemetine; filled diamonds – DHE1; empty diamonds – DHE3.

Article Snippet: Viral genome copy number was determined by reverse transcription coupled with quantitative PCR (RT-qPCR) using Quantitative Genomic RNA from Betacoronavirus 1 Strain OC43 (ATCC; VR-1558DQ) as a standard.

Techniques: Activity Assay, Virus, Infection, Isolation, Quantitative RT-PCR, MTS Assay, Inhibition

Journal: Virus Research

Article Title: Mechanisms of antiviral action and toxicities of ipecac alkaloids: Emetine and dehydroemetine exhibit anti-coronaviral activities at non-cardiotoxic concentrations

doi: 10.1016/j.virusres.2024.199322

Figure Lengend Snippet: Selectivity of ipecac alkaloids toward HCoV-OC43 virus in Vero E6 cells and their effects on protein synthesis in this cell line. Quantification of data as such shown in Figs. 4 and 5 . Conditions when drugs were introduced for 1 h before viral absorption and present 72 h following infections are indicated as 1 hbi/72 hpi. Protein synthesis inhibition was identified by puromycin labeling and shown as a percent signal of puromycilated proteins in comparison with untreated control.

Article Snippet: Viral genome copy number was determined by reverse transcription coupled with quantitative PCR (RT-qPCR) using Quantitative Genomic RNA from Betacoronavirus 1 Strain OC43 (ATCC; VR-1558DQ) as a standard.

Techniques: Virus, Inhibition, Labeling, Comparison, Control

Journal: Heliyon

Article Title: Direct NP- A cost-effective extraction-free RT-qPCR based test for SARS-CoV-2

doi: 10.1016/j.heliyon.2022.e09735

Figure Lengend Snippet: Cross-reactivity for Direct NP. For each viral genome, we tested three technical replicate.

Article Snippet: Betacoronavirus 1 , OC43 , ATCC#VR-1558DQ , 0/3 , Negative.

Techniques: