venom phosphodiesterase vph  (Worthington Biochemical)


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    Name:
    Phosphodiesterase I
    Description:
    Purified by the method of Williams Sung and Laskowski J Biol Chem 236 1130 1961 Further treated to inactivate contaminating 5 nucleotidase activity according to Sulkowski and Laskowski Biochim Biophys Acta 240 443 1961 Lyophilized in vials
    Catalog Number:
    LS003926
    Price:
    79
    Source:
    Crotalus adamanteus Venom
    Size:
    100 un
    Cas Number:
    9025.82.5
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    Structured Review

    Worthington Biochemical venom phosphodiesterase vph
    Purified by the method of Williams Sung and Laskowski J Biol Chem 236 1130 1961 Further treated to inactivate contaminating 5 nucleotidase activity according to Sulkowski and Laskowski Biochim Biophys Acta 240 443 1961 Lyophilized in vials
    https://www.bioz.com/result/venom phosphodiesterase vph/product/Worthington Biochemical
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    venom phosphodiesterase vph - by Bioz Stars, 2021-04
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Selective alkylation of T–T mismatched DNA using vinyldiaminotriazine–acridine conjugate
    Article Snippet: Enzymatic hydrolysis of the alkylated ODN5 and ODN6 The alkylated ODN 5 and ODN 6 were synthesized with ODN 3 (600 nmol), ODN 4 (Y = dT) (600 nmol) and VDAT–acridine conjugate 2 (3.0 μmol) in the same manner as the alkylation conditions with ODN 1 (X = dT) and ODN 2 (Y = dT) and purified by RP-HPLC (CAPCELL PAL MG-II, Shiseido, 4.6 × 250 mm, solvent A: 50 mM TEAA, solvent B: MeCN, linear gradient: B 10–30%/ 20 min, flow rate: 1.0 ml/min, temperature: 40°C). .. A solution (100 μl) of the alkylated ODN 5 or ODN 6 (100 μM), alkaline phosphatase (0.03 U/μl, Takara Bio Inc.) and phosphodiesterase I (0.03 U/μl, Worthington Biochemical Corp.) in alkaline phosphatase buffer was incubated at 37°C for 1 h. The reaction mixture was heated at 90°C for 3 min, cooled to 0°C for 3 min and analyzed by RP-HPLC (CAPCELL PAL MG-II, Shiseido, 4.6 × 250 mm, solvent A: 50 mM TEAA for analysis or HCOONH4 for purification, solvent B: MeCN, linear gradient: B 5–40%/ 30 min, flow rate: 1.0 ml/min). .. Determination of the alkylation position of dT* by NMR The alkylated thymidine dT* obtained by enzymatic hydrolysis of the alkylated ODN 5 and ODN 6 was dissolved in DMSO-d6 containing 10 μM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS), the final concentration being 400 μM.

    Purification:

    Article Title: Selective alkylation of T–T mismatched DNA using vinyldiaminotriazine–acridine conjugate
    Article Snippet: Enzymatic hydrolysis of the alkylated ODN5 and ODN6 The alkylated ODN 5 and ODN 6 were synthesized with ODN 3 (600 nmol), ODN 4 (Y = dT) (600 nmol) and VDAT–acridine conjugate 2 (3.0 μmol) in the same manner as the alkylation conditions with ODN 1 (X = dT) and ODN 2 (Y = dT) and purified by RP-HPLC (CAPCELL PAL MG-II, Shiseido, 4.6 × 250 mm, solvent A: 50 mM TEAA, solvent B: MeCN, linear gradient: B 10–30%/ 20 min, flow rate: 1.0 ml/min, temperature: 40°C). .. A solution (100 μl) of the alkylated ODN 5 or ODN 6 (100 μM), alkaline phosphatase (0.03 U/μl, Takara Bio Inc.) and phosphodiesterase I (0.03 U/μl, Worthington Biochemical Corp.) in alkaline phosphatase buffer was incubated at 37°C for 1 h. The reaction mixture was heated at 90°C for 3 min, cooled to 0°C for 3 min and analyzed by RP-HPLC (CAPCELL PAL MG-II, Shiseido, 4.6 × 250 mm, solvent A: 50 mM TEAA for analysis or HCOONH4 for purification, solvent B: MeCN, linear gradient: B 5–40%/ 30 min, flow rate: 1.0 ml/min). .. Determination of the alkylation position of dT* by NMR The alkylated thymidine dT* obtained by enzymatic hydrolysis of the alkylated ODN 5 and ODN 6 was dissolved in DMSO-d6 containing 10 μM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS), the final concentration being 400 μM.

    Flow Cytometry:

    Article Title: Selective alkylation of T–T mismatched DNA using vinyldiaminotriazine–acridine conjugate
    Article Snippet: Enzymatic hydrolysis of the alkylated ODN5 and ODN6 The alkylated ODN 5 and ODN 6 were synthesized with ODN 3 (600 nmol), ODN 4 (Y = dT) (600 nmol) and VDAT–acridine conjugate 2 (3.0 μmol) in the same manner as the alkylation conditions with ODN 1 (X = dT) and ODN 2 (Y = dT) and purified by RP-HPLC (CAPCELL PAL MG-II, Shiseido, 4.6 × 250 mm, solvent A: 50 mM TEAA, solvent B: MeCN, linear gradient: B 10–30%/ 20 min, flow rate: 1.0 ml/min, temperature: 40°C). .. A solution (100 μl) of the alkylated ODN 5 or ODN 6 (100 μM), alkaline phosphatase (0.03 U/μl, Takara Bio Inc.) and phosphodiesterase I (0.03 U/μl, Worthington Biochemical Corp.) in alkaline phosphatase buffer was incubated at 37°C for 1 h. The reaction mixture was heated at 90°C for 3 min, cooled to 0°C for 3 min and analyzed by RP-HPLC (CAPCELL PAL MG-II, Shiseido, 4.6 × 250 mm, solvent A: 50 mM TEAA for analysis or HCOONH4 for purification, solvent B: MeCN, linear gradient: B 5–40%/ 30 min, flow rate: 1.0 ml/min). .. Determination of the alkylation position of dT* by NMR The alkylated thymidine dT* obtained by enzymatic hydrolysis of the alkylated ODN 5 and ODN 6 was dissolved in DMSO-d6 containing 10 μM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS), the final concentration being 400 μM.

    other:

    Article Title: Mono(ADP-ribosyl)ation of 2?-deoxyguanosine residue in DNA by an apoptosis-inducing protein, pierisin-1, from cabbage butterfly
    Article Snippet: Micrococcal nuclease and phosphodiesterase I ( Crotalus adamanteus venom) and II (bovine spleen) were obtained from Worthington. β-[ Adenylate -32 P]NAD ([32 P]NAD) was purchased from NEN.

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  • 95
    Worthington Biochemical venom phosphodiesterase vph
    Venom Phosphodiesterase Vph, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/venom phosphodiesterase vph/product/Worthington Biochemical
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    venom phosphodiesterase vph - by Bioz Stars, 2021-04
    95/100 stars
      Buy from Supplier

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