alkaline phosphatase alp  (Worthington Biochemical)


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    • 92
    Name:
    Phosphodiesterase I
    Description:
    Purified by the method of Williams Sung and Laskowski J Biol Chem 236 1130 1961 Further treated to inactivate contaminating 5 nucleotidase activity according to Sulkowski and Laskowski Biochim Biophys Acta 240 443 1961 Lyophilized in vials
    Catalog Number:
    ls003926
    Price:
    79
    Size:
    100 un
    Source:
    Crotalus adamanteus Venom
    Cas Number:
    9025.82.5
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    Structured Review

    Worthington Biochemical alkaline phosphatase alp
    Optimization of hydrolysis conditions for detecting stable 4-OHEN-DNA adducts. A) Method I, incubation with NP1 and <t>ALP</t> together in 25 mM ammonium acetate (pH 5.3) for 45 min at 37 °C. Method II, the DNA solutions were incubated with NP1 for 4 h in ammonium acetate at 55 °C, and then ALP and <t>VPH</t> were added to mixture for 4 h at 37 °C after pH adjustment to 9.8 by 0.1 M diethanolamine. B) Equal amounts of calf thymus DNA were hydrolyzed using Method I and Method II. The hydrolysates were analyzed by LC-MS/MS and compared by total relative intensity of each adduct peak.
    Purified by the method of Williams Sung and Laskowski J Biol Chem 236 1130 1961 Further treated to inactivate contaminating 5 nucleotidase activity according to Sulkowski and Laskowski Biochim Biophys Acta 240 443 1961 Lyophilized in vials
    https://www.bioz.com/result/alkaline phosphatase alp/product/Worthington Biochemical
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    alkaline phosphatase alp - by Bioz Stars, 2020-10
    92/100 stars

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    Images

    1) Product Images from "Development of a Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry Method for Analysis of Stable 4-Hydroxyequilenin-DNA Adducts in Human Breast Cancer Cells"

    Article Title: Development of a Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry Method for Analysis of Stable 4-Hydroxyequilenin-DNA Adducts in Human Breast Cancer Cells

    Journal: Chemical research in toxicology

    doi: 10.1021/tx900063g

    Optimization of hydrolysis conditions for detecting stable 4-OHEN-DNA adducts. A) Method I, incubation with NP1 and ALP together in 25 mM ammonium acetate (pH 5.3) for 45 min at 37 °C. Method II, the DNA solutions were incubated with NP1 for 4 h in ammonium acetate at 55 °C, and then ALP and VPH were added to mixture for 4 h at 37 °C after pH adjustment to 9.8 by 0.1 M diethanolamine. B) Equal amounts of calf thymus DNA were hydrolyzed using Method I and Method II. The hydrolysates were analyzed by LC-MS/MS and compared by total relative intensity of each adduct peak.
    Figure Legend Snippet: Optimization of hydrolysis conditions for detecting stable 4-OHEN-DNA adducts. A) Method I, incubation with NP1 and ALP together in 25 mM ammonium acetate (pH 5.3) for 45 min at 37 °C. Method II, the DNA solutions were incubated with NP1 for 4 h in ammonium acetate at 55 °C, and then ALP and VPH were added to mixture for 4 h at 37 °C after pH adjustment to 9.8 by 0.1 M diethanolamine. B) Equal amounts of calf thymus DNA were hydrolyzed using Method I and Method II. The hydrolysates were analyzed by LC-MS/MS and compared by total relative intensity of each adduct peak.

    Techniques Used: Incubation, ALP Assay, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    Related Articles

    Concentration Assay:

    Article Title: Solution Structure of the B3 DNA Binding Domain of the Arabidopsis Cold-Responsive Transcription Factor RAV1 W⃞
    Article Snippet: .. The concentration of the double-stranded DNA was determined using an extinction coefficient calculated after digestion of the strands with phosphodiesterase I (Worthington Biochemical, Lakewood, NJ). .. The HOMOLOGY module of Insight II (Accelrys, San Diego, CA) was used for the structural modeling of the ARF1- and ABI3-B3 domains based on the sequence homology to RAV1-B3.

    Incubation:

    Article Title: Functional characterization of the YmcB and YqeV tRNA methylthiotransferases of Bacillus subtilis
    Article Snippet: .. Four microliter 0.1 M ammonium acetate pH 5.3 and 8 U Nuclease P1 (Sigma-Aldrich) were added, and samples were incubated at 45°C for 2 h. Four microliters of 0.1 M ammonium bicarbonate and 0.1 U Phosphodiesterase I (Worthington Biochemical, Lakewood, NJ, USA) were added, and samples were incubated at 37°C for 2 h. Five units Antarctic phosphatase (New England Biolabs) were added, and samples were incubated at 37°C for 1 h. .. Liquid chromatography and ESI-MS analysis Nucleosides were separated, at room temperature, on a Hitachi HPLC system (L-7100 pump) with UV detection at 260 nm (L-7400 UV detector).

    Article Title: Selective alkylation of T–T mismatched DNA using vinyldiaminotriazine–acridine conjugate
    Article Snippet: .. A solution (100 μl) of the alkylated ODN 5 or ODN 6 (100 μM), alkaline phosphatase (0.03 U/μl, Takara Bio Inc.) and phosphodiesterase I (0.03 U/μl, Worthington Biochemical Corp.) in alkaline phosphatase buffer was incubated at 37°C for 1 h. The reaction mixture was heated at 90°C for 3 min, cooled to 0°C for 3 min and analyzed by RP-HPLC (CAPCELL PAL MG-II, Shiseido, 4.6 × 250 mm, solvent A: 50 mM TEAA for analysis or HCOONH4 for purification, solvent B: MeCN, linear gradient: B 5–40%/ 30 min, flow rate: 1.0 ml/min). .. The alkylated thymidine dT* obtained by enzymatic hydrolysis of the alkylated ODN 5 and ODN 6 was dissolved in DMSO-d6 containing 10 μM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS), the final concentration being 400 μM.

    other:

    Article Title: Mono(ADP-ribosyl)ation of 2?-deoxyguanosine residue in DNA by an apoptosis-inducing protein, pierisin-1, from cabbage butterfly
    Article Snippet: Micrococcal nuclease and phosphodiesterase I ( Crotalus adamanteus venom) and II (bovine spleen) were obtained from Worthington. β-[ Adenylate -32 P]NAD ([32 P]NAD) was purchased from NEN.

    Flow Cytometry:

    Article Title: Selective alkylation of T–T mismatched DNA using vinyldiaminotriazine–acridine conjugate
    Article Snippet: .. A solution (100 μl) of the alkylated ODN 5 or ODN 6 (100 μM), alkaline phosphatase (0.03 U/μl, Takara Bio Inc.) and phosphodiesterase I (0.03 U/μl, Worthington Biochemical Corp.) in alkaline phosphatase buffer was incubated at 37°C for 1 h. The reaction mixture was heated at 90°C for 3 min, cooled to 0°C for 3 min and analyzed by RP-HPLC (CAPCELL PAL MG-II, Shiseido, 4.6 × 250 mm, solvent A: 50 mM TEAA for analysis or HCOONH4 for purification, solvent B: MeCN, linear gradient: B 5–40%/ 30 min, flow rate: 1.0 ml/min). .. The alkylated thymidine dT* obtained by enzymatic hydrolysis of the alkylated ODN 5 and ODN 6 was dissolved in DMSO-d6 containing 10 μM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS), the final concentration being 400 μM.

    Purification:

    Article Title: Selective alkylation of T–T mismatched DNA using vinyldiaminotriazine–acridine conjugate
    Article Snippet: .. A solution (100 μl) of the alkylated ODN 5 or ODN 6 (100 μM), alkaline phosphatase (0.03 U/μl, Takara Bio Inc.) and phosphodiesterase I (0.03 U/μl, Worthington Biochemical Corp.) in alkaline phosphatase buffer was incubated at 37°C for 1 h. The reaction mixture was heated at 90°C for 3 min, cooled to 0°C for 3 min and analyzed by RP-HPLC (CAPCELL PAL MG-II, Shiseido, 4.6 × 250 mm, solvent A: 50 mM TEAA for analysis or HCOONH4 for purification, solvent B: MeCN, linear gradient: B 5–40%/ 30 min, flow rate: 1.0 ml/min). .. The alkylated thymidine dT* obtained by enzymatic hydrolysis of the alkylated ODN 5 and ODN 6 was dissolved in DMSO-d6 containing 10 μM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS), the final concentration being 400 μM.

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    • venom phosphodiesterase i vph  (Worthington Biochemical)
    95
    Worthington Biochemical venom phosphodiesterase i vph
    Venom Phosphodiesterase I Vph, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/venom phosphodiesterase i vph/product/Worthington Biochemical
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    venom phosphodiesterase i vph - by Bioz Stars, 2020-10
    95/100 stars
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