venom phosphodiesterase vph  (Worthington Biochemical)


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    Name:
    Phosphodiesterase I
    Description:
    Purified by the method of Williams Sung and Laskowski J Biol Chem 236 1130 1961 Further treated to inactivate contaminating 5 nucleotidase activity according to Sulkowski and Laskowski Biochim Biophys Acta 240 443 1961 Lyophilized in vials
    Catalog Number:
    LS003926
    Price:
    79
    Source:
    Crotalus adamanteus Venom
    Size:
    100 un
    Cas Number:
    9025.82.5
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    Structured Review

    Worthington Biochemical venom phosphodiesterase vph
    Purified by the method of Williams Sung and Laskowski J Biol Chem 236 1130 1961 Further treated to inactivate contaminating 5 nucleotidase activity according to Sulkowski and Laskowski Biochim Biophys Acta 240 443 1961 Lyophilized in vials
    https://www.bioz.com/result/venom phosphodiesterase vph/product/Worthington Biochemical
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    venom phosphodiesterase vph - by Bioz Stars, 2021-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Combined exposure to polychlorinated biphenyls and high-fat diet modifies the global epitranscriptomic landscape in mouse liver
    Article Snippet: .. The mixture was incubated at 37°C for 4 h. After removing nuclease S1 using a Microcon centrifugal filter (Microcon YM-10, Sigma-Aldrich, St. Louis, MO), the pH of the solution was adjusted to basic using 2.5 µl of a mixture (1 mM MgCl2 , 0.1 mM ZnCl2, 1 mM spermidine, 50 mM Tris-HCl, pH = 9.3), and 0.5 units of phosphodiesterase I (Worthington Biochemical Corporation, Lakewood, NJ) was added. ..

    Mass Spectrometry:

    Article Title: Complete chemical structures of human mitochondrial tRNAs
    Article Snippet: .. RNA mass spectrometry For nucleoside analysis of tRNAs , the isolated tRNA (10 pmol) was digested into nucleosides 5′-monophosphate with 0.2 U nuclease P1 (FUJIFILM Wako Pure Chemical) in 10 mM ammonium acetate (pH 5.3) at 37 °C for 60 min, followed by adding 0.1 U phosphodiesterase I (Worthington) in 50 mM ammonium bicarbonate (to adjust pH from 5.3 to 8.2) and incubating at 37 °C for 60 min. ..

    Isolation:

    Article Title: Complete chemical structures of human mitochondrial tRNAs
    Article Snippet: .. RNA mass spectrometry For nucleoside analysis of tRNAs , the isolated tRNA (10 pmol) was digested into nucleosides 5′-monophosphate with 0.2 U nuclease P1 (FUJIFILM Wako Pure Chemical) in 10 mM ammonium acetate (pH 5.3) at 37 °C for 60 min, followed by adding 0.1 U phosphodiesterase I (Worthington) in 50 mM ammonium bicarbonate (to adjust pH from 5.3 to 8.2) and incubating at 37 °C for 60 min. ..

    High Performance Liquid Chromatography:

    Article Title: Comprehensive analysis of translation from overexpressed circular RNAs reveals pervasive translation from linear transcripts
    Article Snippet: .. Digestion of RNA and subsequent HPLC analysis of nucleosides After Poly(A)+ purification of total RNA using oligo d(T)25 Magnetic beads (NEB), the RNA (total RNA, poly(A)+ RNA and poly(A)- RNA) was digested into single nucleosides by incubating with an enzyme mix of Benzonase (Sigma No. E8263), Phosphodiesterase I (Worthington Cat. No. LS003926) and FastAP (Thermo Scientific) for 3 h at 37°C ( ). ..

    Purification:

    Article Title: Comprehensive analysis of translation from overexpressed circular RNAs reveals pervasive translation from linear transcripts
    Article Snippet: .. Digestion of RNA and subsequent HPLC analysis of nucleosides After Poly(A)+ purification of total RNA using oligo d(T)25 Magnetic beads (NEB), the RNA (total RNA, poly(A)+ RNA and poly(A)- RNA) was digested into single nucleosides by incubating with an enzyme mix of Benzonase (Sigma No. E8263), Phosphodiesterase I (Worthington Cat. No. LS003926) and FastAP (Thermo Scientific) for 3 h at 37°C ( ). ..

    Magnetic Beads:

    Article Title: Comprehensive analysis of translation from overexpressed circular RNAs reveals pervasive translation from linear transcripts
    Article Snippet: .. Digestion of RNA and subsequent HPLC analysis of nucleosides After Poly(A)+ purification of total RNA using oligo d(T)25 Magnetic beads (NEB), the RNA (total RNA, poly(A)+ RNA and poly(A)- RNA) was digested into single nucleosides by incubating with an enzyme mix of Benzonase (Sigma No. E8263), Phosphodiesterase I (Worthington Cat. No. LS003926) and FastAP (Thermo Scientific) for 3 h at 37°C ( ). ..

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  • 94
    Worthington Biochemical venom phosphodiesterase vph
    Venom Phosphodiesterase Vph, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/venom phosphodiesterase vph/product/Worthington Biochemical
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    venom phosphodiesterase vph - by Bioz Stars, 2021-10
    94/100 stars
      Buy from Supplier

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