alkaline phosphatase alp (Worthington Biochemical)


92
Name:
Phosphodiesterase I
Description:
Purified by the method of Williams Sung and Laskowski J Biol Chem 236 1130 1961 Further treated to inactivate contaminating 5 nucleotidase activity according to Sulkowski and Laskowski Biochim Biophys Acta 240 443 1961 Lyophilized in vials
Catalog Number:
ls003926
Price:
79
Size:
100 un
Source:
Crotalus adamanteus Venom
Cas Number:
9025.82.5
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Structured Review
Worthington Biochemical
alkaline phosphatase alp

Purified by the method of Williams Sung and Laskowski J Biol Chem 236 1130 1961 Further treated to inactivate contaminating 5 nucleotidase activity according to Sulkowski and Laskowski Biochim Biophys Acta 240 443 1961 Lyophilized in vials
https://www.bioz.com/result/alkaline phosphatase alp/product/Worthington Biochemical
Average 92 stars, based on 8 article reviews
Price from $9.99 to $1999.99

Purified by the method of Williams Sung and Laskowski J Biol Chem 236 1130 1961 Further treated to inactivate contaminating 5 nucleotidase activity according to Sulkowski and Laskowski Biochim Biophys Acta 240 443 1961 Lyophilized in vials
https://www.bioz.com/result/alkaline phosphatase alp/product/Worthington Biochemical
Average 92 stars, based on 8 article reviews
Price from $9.99 to $1999.99
alkaline phosphatase alp - by Bioz Stars,
2021-01
92/100 stars
Related Products / Commonly Used Together
Images
1) Product Images from "Development of a Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry Method for Analysis of Stable 4-Hydroxyequilenin-DNA Adducts in Human Breast Cancer Cells"
Article Title: Development of a Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry Method for Analysis of Stable 4-Hydroxyequilenin-DNA Adducts in Human Breast Cancer Cells
Journal: Chemical research in toxicology
doi: 10.1021/tx900063g

Figure Legend Snippet: Optimization of hydrolysis conditions for detecting stable 4-OHEN-DNA adducts. A) Method I, incubation with NP1 and ALP together in 25 mM ammonium acetate (pH 5.3) for 45 min at 37 °C. Method II, the DNA solutions were incubated with NP1 for 4 h in ammonium acetate at 55 °C, and then ALP and VPH were added to mixture for 4 h at 37 °C after pH adjustment to 9.8 by 0.1 M diethanolamine. B) Equal amounts of calf thymus DNA were hydrolyzed using Method I and Method II. The hydrolysates were analyzed by LC-MS/MS and compared by total relative intensity of each adduct peak.
Techniques Used: Incubation, ALP Assay, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry
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Incubation:Article Title: Functional characterization of the YmcB and YqeV tRNA methylthiotransferases of Bacillus subtilis Article Snippet: .. Four microliter 0.1 M ammonium acetate pH 5.3 and 8 U Nuclease P1 (Sigma-Aldrich) were added, and samples were incubated at 45°C for 2 h. Four microliters of 0.1 M ammonium bicarbonate and 0.1 U Article Title: Selective alkylation of T–T mismatched DNA using vinyldiaminotriazine–acridine conjugate Article Snippet: .. A solution (100 μl) of the alkylated ODN 5 or ODN 6 (100 μM), alkaline phosphatase (0.03 U/μl, Takara Bio Inc.) and other:Article Title: Mono(ADP-ribosyl)ation of 2?-deoxyguanosine residue in DNA by an apoptosis-inducing protein, pierisin-1, from cabbage butterfly Article Snippet: Micrococcal nuclease and Flow Cytometry:Article Title: Selective alkylation of T–T mismatched DNA using vinyldiaminotriazine–acridine conjugate Article Snippet: .. A solution (100 μl) of the alkylated ODN 5 or ODN 6 (100 μM), alkaline phosphatase (0.03 U/μl, Takara Bio Inc.) and Purification:Article Title: Selective alkylation of T–T mismatched DNA using vinyldiaminotriazine–acridine conjugate Article Snippet: .. A solution (100 μl) of the alkylated ODN 5 or ODN 6 (100 μM), alkaline phosphatase (0.03 U/μl, Takara Bio Inc.) and |