collagenase type ii  (Worthington Biochemical)


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    Name:
    Collagenase Type 2
    Description:
    Prepared to contain higher clostripain activity Suggested for bone heart liver thyroid and salivary primary cell isolation Supplied as a dialyzed lyophilized powder
    Catalog Number:
    LS004174
    Price:
    35
    Source:
    Clostridium histolyticum
    Size:
    100 mg
    Cas Number:
    9001.12.1
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    Structured Review

    Worthington Biochemical collagenase type ii
    Prepared to contain higher clostripain activity Suggested for bone heart liver thyroid and salivary primary cell isolation Supplied as a dialyzed lyophilized powder
    https://www.bioz.com/result/collagenase type ii/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase type ii - by Bioz Stars, 2021-07
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Activation of intervertebral disc cells by co-culture with notochordal cells, conditioned medium and hypoxia
    Article Snippet: .. Porcine and bovine NP tissues were digested by 0.19% pronase (Roche, Basel, Switzerland) for 1 hour and subsequent collagenase type 2 at a concentration of 32 U/mL in 25 mL of HG-DMEM supplemented with 10% FCS (Worthington, London, UK) overnight (~14 hours) at 37°C and standard culture conditions (5% CO2 and 100% humidity) [ , ]. .. Cell separation using micro sieves and cytometry using FACS Cells isolated from porcine NP tissues were separated with three cell sieves, i.e., cells > 20 μm, 8-20 μm and cells < 8 μm (CellMicroSieves, BioDesign Inc., New York, USA) [ ] with the goal to identify NPC and NC.

    Mouse Assay:

    Article Title: The kallikrein-kinin system is falling into pieces: bradykinin fragments are biological active peptidesAngiotensin-( - Structure-function studies of Tityus serrulatus Hypotensin-I (TsHpt-I): A new agonist of B(
    Article Snippet: Cells were maintained with DMEM-F12 cell media supplemented by 10% FBS and 1% of antibiotic/antimycotic solution at 37°C until usage. .. Mice ventricular adult cardiomyocytes were isolated by collagenase type-2 (Worthington), as previously shown ( ) and kept in Tyrode media (1.3 x 10−1 mol.L-1 NaCl; 5.4 x 10−3 mol.L-1 KCl; 2,5 x 10−2 mol.L-1 HEPES; 5 x 10−4 mol.L-1 MgCl2 ; 3.3 x 10−4 mol.L-1 NaH2 PO4 ; 1.8 x 10−3 mol.L-1 mM CaCl2 ; 2.2 x 10−2 mol.L-1 glucose) until usage. .. For NO quantification, cells were starved with Hank’s Balanced Salt Solution (HBSS) for 1 hour and then loaded with DAF-FM diacetate (Thermo-Fisher) at a final concentration of 5 x 10−6 mol.L-1 for 30 minutes.

    Article Title: Macrophages redirect phagocytosis by non-professional phagocytes and influence inflammation
    Article Snippet: .. For lung harvests, mice were perfused through the right ventricle with PBS and the lungs were carefully excised and placed in type 2 collagenase (Worthington Biochemical Corporation) dissolved in HBSS containing Ca2+ and Mg2+ . .. The lung homogenate was then passed through a 70μm nylon strainer, spun down and treated with red blood cell lysis buffer (Sigma-Aldrich) for 5 minutes.

    Article Title: Protective role of angiotensin II type 2 receptor signaling in a mouse model of pancreatic fibrosis
    Article Snippet: .. Briefly, the dissected pancreases from 10 mice were finely chopped and placed in digestion solution containing 0.03% collagenase type 2 (Worthington Biomedical, Lakewood, NJ) and 0.1% DNAse I (Sigma). .. After digestion for 30 min at 37°C with shaking, cells were mechanically dispersed by pipetting, and undigested tissue was removed by filtration through a nylon mesh.

    Isolation:

    Article Title: The kallikrein-kinin system is falling into pieces: bradykinin fragments are biological active peptidesAngiotensin-( - Structure-function studies of Tityus serrulatus Hypotensin-I (TsHpt-I): A new agonist of B(
    Article Snippet: Cells were maintained with DMEM-F12 cell media supplemented by 10% FBS and 1% of antibiotic/antimycotic solution at 37°C until usage. .. Mice ventricular adult cardiomyocytes were isolated by collagenase type-2 (Worthington), as previously shown ( ) and kept in Tyrode media (1.3 x 10−1 mol.L-1 NaCl; 5.4 x 10−3 mol.L-1 KCl; 2,5 x 10−2 mol.L-1 HEPES; 5 x 10−4 mol.L-1 MgCl2 ; 3.3 x 10−4 mol.L-1 NaH2 PO4 ; 1.8 x 10−3 mol.L-1 mM CaCl2 ; 2.2 x 10−2 mol.L-1 glucose) until usage. .. For NO quantification, cells were starved with Hank’s Balanced Salt Solution (HBSS) for 1 hour and then loaded with DAF-FM diacetate (Thermo-Fisher) at a final concentration of 5 x 10−6 mol.L-1 for 30 minutes.

    Activity Assay:

    Article Title: Silk Fiber-Reinforced Hyaluronic Acid-Based Hydrogel for Cartilage Tissue Engineering
    Article Snippet: Articular Chondrocyte Isolation and Culture Human chondrocytes (hCh) (n = 6) were isolated from the femoral heads of patients (age 63 ± 5.8 years old) undergoing hip replacement surgery (Ethic votum was obtained from local committee of the University Kiel, Code: D558/19, Date: 5 December 2019). .. The cartilage tissue was dissected into small pieces and washed twice with phosphate buffer saline (PBS; Sigma-Aldrich, Buchs, Switzerland) for 15 min. Cartilage pieces were first digested with 0.1% pronase (Roche, Mannheim, Germany) for 2 h and then with type 2 collagenase (Worthington, Lakewood, NJ, USA) with an enzyme activity of 600 U/mL for 14 h. Digestion solutions were prepared in Dulbecco’s modified Eagle’s medium (DMEM; Biochrom, Berlin, Germany) and the digestions were carried out at 37 °C in an atmosphere of 5% CO2 . ..

    Modification:

    Article Title: Silk Fiber-Reinforced Hyaluronic Acid-Based Hydrogel for Cartilage Tissue Engineering
    Article Snippet: Articular Chondrocyte Isolation and Culture Human chondrocytes (hCh) (n = 6) were isolated from the femoral heads of patients (age 63 ± 5.8 years old) undergoing hip replacement surgery (Ethic votum was obtained from local committee of the University Kiel, Code: D558/19, Date: 5 December 2019). .. The cartilage tissue was dissected into small pieces and washed twice with phosphate buffer saline (PBS; Sigma-Aldrich, Buchs, Switzerland) for 15 min. Cartilage pieces were first digested with 0.1% pronase (Roche, Mannheim, Germany) for 2 h and then with type 2 collagenase (Worthington, Lakewood, NJ, USA) with an enzyme activity of 600 U/mL for 14 h. Digestion solutions were prepared in Dulbecco’s modified Eagle’s medium (DMEM; Biochrom, Berlin, Germany) and the digestions were carried out at 37 °C in an atmosphere of 5% CO2 . ..

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  • 99
    Worthington Biochemical collagenase type ii
    Collagenase Type Ii, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase type ii/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase type ii - by Bioz Stars, 2021-07
    99/100 stars
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