pancreata  (Worthington Biochemical)


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    Name:
    Collagenase Type 4
    Description:
    Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity Suggested for pancreatic islet primary isolation A dialyzed lyophilized powder
    Catalog Number:
    ls004186
    Price:
    35
    Size:
    100 mg
    Source:
    Clostridium histolyticum
    Cas Number:
    9001.12.1
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    Structured Review

    Worthington Biochemical pancreata
    iαβTs expand in PDA. (a) CD45 + leukocytes infiltrating day 21 orthotopic KPC tumors, normal pancreas, and spleens in WT mice were gated and tested for the frequency of TCRβ + CD4 – CD8 – NK1.1 – iαβTs. Representative contour plots and quantitative data are shown (n=10). (b) CD45 + TCRβ + NK1.1 – leukocytes from <t>pancreata</t> and spleens of 6 month-old KC mice were gated and tested for co-expression CD4 and CD8. Representative contour plots are shown (n=5). (c) Multiplex IHC of human PDA and adjacent normal pancreas were stained for CK19, CD3, CD4, and CD8. The frequency of CD3 + CD4 – CD8 – cells were quantified and representative images are shown. (d) Orthotopic KPC tumors were harvested from WT mice on day 21. CD45 + CD3 + leukocytes were purified by FACS and analyzed by single cell RNAseq. The distribution of cellular clusters was determined using the t-Distributed Stochastic Neighbor Embedding (t-SNE) algorithm. Each cluster is identified by a distinct color. Percent cellular abundance in each cluster is indicated. (e) Orthotopic KPC tumors were harvested from WT mice on days 7, 14, or 21 after tumor cell implantation and tumor-infiltrating CD45 + TCRβ + CD4 – CD8 – leukocytes were gated and tested for expression of NK1.1. Representative contour plots from days 7 and 21 and quantitative data comparing frequency of tumor-infiltrating iαβT per NKT cells at all time points are shown (n=5/time point). (f) Paraffin-embedded sections made from tumors of mice serially treated with anti-TCRγ/δ and NK1.1 depleting antibodies were tested for co-expression of Hematoxylin, CD3, CD4, and CD8 in the PDA TME. (g) CD45 + TCRβ + NK1.1 – leukocytes infiltrating orthotopic KPC tumors in WT and Fas lpr mice were gated and tested for expression of CD4 and CD8. Representative contour plots and quantitative data are shown (n=5/group). (h) The thymus from 6-month old WT and KC mice were harvested and CD45 + TCRβ + thymocytes were gated and tested for expression of CD4 and CD8. The frequency of iαβTs in the thymus was calculated (n=5/group). (i) CD4 + T cells, CD8 + T cells, or iαβTs were harvested from CD45.1 mice and transferred i.v. to orthotopic PDA-bearing CD45.2 mice. PDA tumors were harvested at 96 hours and CD45.1 + cells were gated and tested for CD4 and CD8 expression. Representative contour plots are shown (n=5/group). (j) WT mice were orthotopically administered KPC tumor cells and sacrificed on day 21. PDA-infiltrating CD4 + T cells, CD8 + T cells, and iαβTs were assayed for Ki67 proliferative index. Representative contour plots and quantitative data are shown (n=5). (k) Splenic and orthotopic PDA-infiltrating iαβTs were tested on day 21 for expression of CCR2, CCR5, and CCR6 (n=5). (l) The frequency of PDA-infiltrating iαβTs was tested on day 21 in WT, CCR2 –/– , CCR5 –/– , and CCR6 –/– hosts (n=5/group). All experiments were repeated at least 3 times (*p
    Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity Suggested for pancreatic islet primary isolation A dialyzed lyophilized powder
    https://www.bioz.com/result/pancreata/product/Worthington Biochemical
    Average 92 stars, based on 729 article reviews
    Price from $9.99 to $1999.99
    pancreata - by Bioz Stars, 2020-08
    92/100 stars

    Images

    1) Product Images from "Innate αβ T cells Mediate Anti-tumor Immunity by Orchestrating Immunogenic Macrophage Programming"

    Article Title: Innate αβ T cells Mediate Anti-tumor Immunity by Orchestrating Immunogenic Macrophage Programming

    Journal: Cancer discovery

    doi: 10.1158/2159-8290.CD-19-0161

    iαβTs expand in PDA. (a) CD45 + leukocytes infiltrating day 21 orthotopic KPC tumors, normal pancreas, and spleens in WT mice were gated and tested for the frequency of TCRβ + CD4 – CD8 – NK1.1 – iαβTs. Representative contour plots and quantitative data are shown (n=10). (b) CD45 + TCRβ + NK1.1 – leukocytes from pancreata and spleens of 6 month-old KC mice were gated and tested for co-expression CD4 and CD8. Representative contour plots are shown (n=5). (c) Multiplex IHC of human PDA and adjacent normal pancreas were stained for CK19, CD3, CD4, and CD8. The frequency of CD3 + CD4 – CD8 – cells were quantified and representative images are shown. (d) Orthotopic KPC tumors were harvested from WT mice on day 21. CD45 + CD3 + leukocytes were purified by FACS and analyzed by single cell RNAseq. The distribution of cellular clusters was determined using the t-Distributed Stochastic Neighbor Embedding (t-SNE) algorithm. Each cluster is identified by a distinct color. Percent cellular abundance in each cluster is indicated. (e) Orthotopic KPC tumors were harvested from WT mice on days 7, 14, or 21 after tumor cell implantation and tumor-infiltrating CD45 + TCRβ + CD4 – CD8 – leukocytes were gated and tested for expression of NK1.1. Representative contour plots from days 7 and 21 and quantitative data comparing frequency of tumor-infiltrating iαβT per NKT cells at all time points are shown (n=5/time point). (f) Paraffin-embedded sections made from tumors of mice serially treated with anti-TCRγ/δ and NK1.1 depleting antibodies were tested for co-expression of Hematoxylin, CD3, CD4, and CD8 in the PDA TME. (g) CD45 + TCRβ + NK1.1 – leukocytes infiltrating orthotopic KPC tumors in WT and Fas lpr mice were gated and tested for expression of CD4 and CD8. Representative contour plots and quantitative data are shown (n=5/group). (h) The thymus from 6-month old WT and KC mice were harvested and CD45 + TCRβ + thymocytes were gated and tested for expression of CD4 and CD8. The frequency of iαβTs in the thymus was calculated (n=5/group). (i) CD4 + T cells, CD8 + T cells, or iαβTs were harvested from CD45.1 mice and transferred i.v. to orthotopic PDA-bearing CD45.2 mice. PDA tumors were harvested at 96 hours and CD45.1 + cells were gated and tested for CD4 and CD8 expression. Representative contour plots are shown (n=5/group). (j) WT mice were orthotopically administered KPC tumor cells and sacrificed on day 21. PDA-infiltrating CD4 + T cells, CD8 + T cells, and iαβTs were assayed for Ki67 proliferative index. Representative contour plots and quantitative data are shown (n=5). (k) Splenic and orthotopic PDA-infiltrating iαβTs were tested on day 21 for expression of CCR2, CCR5, and CCR6 (n=5). (l) The frequency of PDA-infiltrating iαβTs was tested on day 21 in WT, CCR2 –/– , CCR5 –/– , and CCR6 –/– hosts (n=5/group). All experiments were repeated at least 3 times (*p
    Figure Legend Snippet: iαβTs expand in PDA. (a) CD45 + leukocytes infiltrating day 21 orthotopic KPC tumors, normal pancreas, and spleens in WT mice were gated and tested for the frequency of TCRβ + CD4 – CD8 – NK1.1 – iαβTs. Representative contour plots and quantitative data are shown (n=10). (b) CD45 + TCRβ + NK1.1 – leukocytes from pancreata and spleens of 6 month-old KC mice were gated and tested for co-expression CD4 and CD8. Representative contour plots are shown (n=5). (c) Multiplex IHC of human PDA and adjacent normal pancreas were stained for CK19, CD3, CD4, and CD8. The frequency of CD3 + CD4 – CD8 – cells were quantified and representative images are shown. (d) Orthotopic KPC tumors were harvested from WT mice on day 21. CD45 + CD3 + leukocytes were purified by FACS and analyzed by single cell RNAseq. The distribution of cellular clusters was determined using the t-Distributed Stochastic Neighbor Embedding (t-SNE) algorithm. Each cluster is identified by a distinct color. Percent cellular abundance in each cluster is indicated. (e) Orthotopic KPC tumors were harvested from WT mice on days 7, 14, or 21 after tumor cell implantation and tumor-infiltrating CD45 + TCRβ + CD4 – CD8 – leukocytes were gated and tested for expression of NK1.1. Representative contour plots from days 7 and 21 and quantitative data comparing frequency of tumor-infiltrating iαβT per NKT cells at all time points are shown (n=5/time point). (f) Paraffin-embedded sections made from tumors of mice serially treated with anti-TCRγ/δ and NK1.1 depleting antibodies were tested for co-expression of Hematoxylin, CD3, CD4, and CD8 in the PDA TME. (g) CD45 + TCRβ + NK1.1 – leukocytes infiltrating orthotopic KPC tumors in WT and Fas lpr mice were gated and tested for expression of CD4 and CD8. Representative contour plots and quantitative data are shown (n=5/group). (h) The thymus from 6-month old WT and KC mice were harvested and CD45 + TCRβ + thymocytes were gated and tested for expression of CD4 and CD8. The frequency of iαβTs in the thymus was calculated (n=5/group). (i) CD4 + T cells, CD8 + T cells, or iαβTs were harvested from CD45.1 mice and transferred i.v. to orthotopic PDA-bearing CD45.2 mice. PDA tumors were harvested at 96 hours and CD45.1 + cells were gated and tested for CD4 and CD8 expression. Representative contour plots are shown (n=5/group). (j) WT mice were orthotopically administered KPC tumor cells and sacrificed on day 21. PDA-infiltrating CD4 + T cells, CD8 + T cells, and iαβTs were assayed for Ki67 proliferative index. Representative contour plots and quantitative data are shown (n=5). (k) Splenic and orthotopic PDA-infiltrating iαβTs were tested on day 21 for expression of CCR2, CCR5, and CCR6 (n=5). (l) The frequency of PDA-infiltrating iαβTs was tested on day 21 in WT, CCR2 –/– , CCR5 –/– , and CCR6 –/– hosts (n=5/group). All experiments were repeated at least 3 times (*p

    Techniques Used: Mouse Assay, Expressing, Multiplex Assay, Immunohistochemistry, Staining, Purification, FACS

    Related Articles

    Homogenization:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

    Mouse Assay:

    Article Title: Increasing antigen presentation on HSV-1-infected cells increases lesion size but does not alter neural infection or latency
    Article Snippet: .. To prepare single cell suspensions of DRG, DRG were collected from mice from spinal levels T5 to L1, trimmed of excess tissue, and placed directly into 1 ml of 1 mg ml−1 Type IV Collagenase (at least 160 units ml−1 ; Worthington) and 0.03 mg ml−1 DNase (at least 600 units ml−1 ; Roche) in DMEM supplemented with 2 % FCS. .. The DRG were incubated at 37 °C, 200 r.p.m. for 60 min, and gently ground through a 70 µM cell strainer and washed with excess cold PBS containing 2 % FCS.

    Concentration Assay:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

    Incubation:

    Article Title: Excitation and Modulation of TRPA1, TRPV1, and TRPM8 Channel-expressing Sensory Neurons by the Pruritogen Chloroquine *
    Article Snippet: .. DRGs were collected from all cervical, thoracic, and lumbar segments and incubated in calcium- and magnesium-free Hanks' balanced salt solution (Invitrogen) containing 2.5 mg/ml type IV collagenase (Worthington Biochemicals) at 37 °C for 1 h. After incubation, DRGs were washed with DMEM (Invitrogen) followed by trituration with 25-gauge needles to dissociate neurons. .. The dissociated neurons were plated onto coverslips precoated with poly- l -lysine (Sigma) and laminin (BD Biosciences) and cultured in DMEM containing 10% fetal bovine serum (Invitrogen), 2 m m l -glutamine, 100 IU/ml penicillin, and 100 μg/ml streptomycin supplemented with 5 μ m cytosine β- d -arabinofuranoside (Sigma) and 1× N2 supplement (Invitrogen).

    FACS:

    Article Title: RIP1 Kinase Drives Macrophage Mediated Adaptive Immune Tolerance in Pancreatic Cancer
    Article Snippet: .. Briefly, pancreata were placed in cold 2 % FACS (PBS with 2 % FBS) with Collagenase IV (1 mg/mL; Worthington Biochemical, Lakewood, NJ), Trypsin inhibitor (1 mg/mL; EMD Millipore, Billerica, MA) and DNase I (2 U/mL; Promega, Madison, WI), and minced with scissors to sub-millimeter pieces. .. Cell pellets were re-suspended and cell labeling was performed after blocking FcγRIII/II with an anti-CD16/CD32 mAb (eBiosciences, San Diego, CA) by incubating 1x106 cells with 1 μg of fluorescently conjugated mAbs directed against mouse CD44 (IM7), CD206 (C068C2), PD-1 (29F.1A12), CD3 (17A2), CD4 (RM4-5), CD8 (53-6.7), CD45 (30-F11), CD11b (M1/70), CD11c (N418), CD86 (GL-1), Gr1 (RB6-8C5), MHC II (M5/114.15.2), IL-10 (JES5-16E3), IFNγ (XMG1.2), TNFα (MP6-XT22), F4/80 (BM8), ICOS (15F9), CD62L (MEL-14), CD25 (3C7), CD40 (3/23), CD69 (H1.2F3), IL-17A (TC11-18H10.1), TGFβ (TW7-16B4), LFA-1 (H155-78), LAG-3 (C9B7W), pSTAT1 (A15158B), pSTAT3 (13A3-1), pSTAT6 (A15137E; all BioLegend, San Diego, CA), pSTAT5 (47/Stat5(pY694); BD Biosciences), T-bet (eBio4B10), RORγt (AFKJS-9), Granzyme B (NGZB), Perforin (eBioOMAK-D), and FoxP3 (FJK-16s; all eBiosciences).

    Purification:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

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    Worthington Biochemical avian ty 1
    Avian Ty 1, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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