pancreata  (Worthington Biochemical)


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  • 92
    Name:
    Collagenase Type 4
    Description:
    Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity Suggested for pancreatic islet primary isolation A dialyzed lyophilized powder
    Catalog Number:
    ls004186
    Price:
    35
    Size:
    100 mg
    Source:
    Clostridium histolyticum
    Cas Number:
    9001.12.1
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    Structured Review

    Worthington Biochemical pancreata
    iαβTs expand in PDA. (a) CD45 + leukocytes infiltrating day 21 orthotopic KPC tumors, normal pancreas, and spleens in WT mice were gated and tested for the frequency of TCRβ + CD4 – CD8 – NK1.1 – iαβTs. Representative contour plots and quantitative data are shown (n=10). (b) CD45 + TCRβ + NK1.1 – leukocytes from <t>pancreata</t> and spleens of 6 month-old KC mice were gated and tested for co-expression CD4 and CD8. Representative contour plots are shown (n=5). (c) Multiplex IHC of human PDA and adjacent normal pancreas were stained for CK19, CD3, CD4, and CD8. The frequency of CD3 + CD4 – CD8 – cells were quantified and representative images are shown. (d) Orthotopic KPC tumors were harvested from WT mice on day 21. CD45 + CD3 + leukocytes were purified by FACS and analyzed by single cell RNAseq. The distribution of cellular clusters was determined using the t-Distributed Stochastic Neighbor Embedding (t-SNE) algorithm. Each cluster is identified by a distinct color. Percent cellular abundance in each cluster is indicated. (e) Orthotopic KPC tumors were harvested from WT mice on days 7, 14, or 21 after tumor cell implantation and tumor-infiltrating CD45 + TCRβ + CD4 – CD8 – leukocytes were gated and tested for expression of NK1.1. Representative contour plots from days 7 and 21 and quantitative data comparing frequency of tumor-infiltrating iαβT per NKT cells at all time points are shown (n=5/time point). (f) Paraffin-embedded sections made from tumors of mice serially treated with anti-TCRγ/δ and NK1.1 depleting antibodies were tested for co-expression of Hematoxylin, CD3, CD4, and CD8 in the PDA TME. (g) CD45 + TCRβ + NK1.1 – leukocytes infiltrating orthotopic KPC tumors in WT and Fas lpr mice were gated and tested for expression of CD4 and CD8. Representative contour plots and quantitative data are shown (n=5/group). (h) The thymus from 6-month old WT and KC mice were harvested and CD45 + TCRβ + thymocytes were gated and tested for expression of CD4 and CD8. The frequency of iαβTs in the thymus was calculated (n=5/group). (i) CD4 + T cells, CD8 + T cells, or iαβTs were harvested from CD45.1 mice and transferred i.v. to orthotopic PDA-bearing CD45.2 mice. PDA tumors were harvested at 96 hours and CD45.1 + cells were gated and tested for CD4 and CD8 expression. Representative contour plots are shown (n=5/group). (j) WT mice were orthotopically administered KPC tumor cells and sacrificed on day 21. PDA-infiltrating CD4 + T cells, CD8 + T cells, and iαβTs were assayed for Ki67 proliferative index. Representative contour plots and quantitative data are shown (n=5). (k) Splenic and orthotopic PDA-infiltrating iαβTs were tested on day 21 for expression of CCR2, CCR5, and CCR6 (n=5). (l) The frequency of PDA-infiltrating iαβTs was tested on day 21 in WT, CCR2 –/– , CCR5 –/– , and CCR6 –/– hosts (n=5/group). All experiments were repeated at least 3 times (*p
    Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity Suggested for pancreatic islet primary isolation A dialyzed lyophilized powder
    https://www.bioz.com/result/pancreata/product/Worthington Biochemical
    Average 92 stars, based on 1461 article reviews
    Price from $9.99 to $1999.99
    pancreata - by Bioz Stars, 2020-10
    92/100 stars

    Images

    1) Product Images from "Innate αβ T cells Mediate Anti-tumor Immunity by Orchestrating Immunogenic Macrophage Programming"

    Article Title: Innate αβ T cells Mediate Anti-tumor Immunity by Orchestrating Immunogenic Macrophage Programming

    Journal: Cancer discovery

    doi: 10.1158/2159-8290.CD-19-0161

    iαβTs expand in PDA. (a) CD45 + leukocytes infiltrating day 21 orthotopic KPC tumors, normal pancreas, and spleens in WT mice were gated and tested for the frequency of TCRβ + CD4 – CD8 – NK1.1 – iαβTs. Representative contour plots and quantitative data are shown (n=10). (b) CD45 + TCRβ + NK1.1 – leukocytes from pancreata and spleens of 6 month-old KC mice were gated and tested for co-expression CD4 and CD8. Representative contour plots are shown (n=5). (c) Multiplex IHC of human PDA and adjacent normal pancreas were stained for CK19, CD3, CD4, and CD8. The frequency of CD3 + CD4 – CD8 – cells were quantified and representative images are shown. (d) Orthotopic KPC tumors were harvested from WT mice on day 21. CD45 + CD3 + leukocytes were purified by FACS and analyzed by single cell RNAseq. The distribution of cellular clusters was determined using the t-Distributed Stochastic Neighbor Embedding (t-SNE) algorithm. Each cluster is identified by a distinct color. Percent cellular abundance in each cluster is indicated. (e) Orthotopic KPC tumors were harvested from WT mice on days 7, 14, or 21 after tumor cell implantation and tumor-infiltrating CD45 + TCRβ + CD4 – CD8 – leukocytes were gated and tested for expression of NK1.1. Representative contour plots from days 7 and 21 and quantitative data comparing frequency of tumor-infiltrating iαβT per NKT cells at all time points are shown (n=5/time point). (f) Paraffin-embedded sections made from tumors of mice serially treated with anti-TCRγ/δ and NK1.1 depleting antibodies were tested for co-expression of Hematoxylin, CD3, CD4, and CD8 in the PDA TME. (g) CD45 + TCRβ + NK1.1 – leukocytes infiltrating orthotopic KPC tumors in WT and Fas lpr mice were gated and tested for expression of CD4 and CD8. Representative contour plots and quantitative data are shown (n=5/group). (h) The thymus from 6-month old WT and KC mice were harvested and CD45 + TCRβ + thymocytes were gated and tested for expression of CD4 and CD8. The frequency of iαβTs in the thymus was calculated (n=5/group). (i) CD4 + T cells, CD8 + T cells, or iαβTs were harvested from CD45.1 mice and transferred i.v. to orthotopic PDA-bearing CD45.2 mice. PDA tumors were harvested at 96 hours and CD45.1 + cells were gated and tested for CD4 and CD8 expression. Representative contour plots are shown (n=5/group). (j) WT mice were orthotopically administered KPC tumor cells and sacrificed on day 21. PDA-infiltrating CD4 + T cells, CD8 + T cells, and iαβTs were assayed for Ki67 proliferative index. Representative contour plots and quantitative data are shown (n=5). (k) Splenic and orthotopic PDA-infiltrating iαβTs were tested on day 21 for expression of CCR2, CCR5, and CCR6 (n=5). (l) The frequency of PDA-infiltrating iαβTs was tested on day 21 in WT, CCR2 –/– , CCR5 –/– , and CCR6 –/– hosts (n=5/group). All experiments were repeated at least 3 times (*p
    Figure Legend Snippet: iαβTs expand in PDA. (a) CD45 + leukocytes infiltrating day 21 orthotopic KPC tumors, normal pancreas, and spleens in WT mice were gated and tested for the frequency of TCRβ + CD4 – CD8 – NK1.1 – iαβTs. Representative contour plots and quantitative data are shown (n=10). (b) CD45 + TCRβ + NK1.1 – leukocytes from pancreata and spleens of 6 month-old KC mice were gated and tested for co-expression CD4 and CD8. Representative contour plots are shown (n=5). (c) Multiplex IHC of human PDA and adjacent normal pancreas were stained for CK19, CD3, CD4, and CD8. The frequency of CD3 + CD4 – CD8 – cells were quantified and representative images are shown. (d) Orthotopic KPC tumors were harvested from WT mice on day 21. CD45 + CD3 + leukocytes were purified by FACS and analyzed by single cell RNAseq. The distribution of cellular clusters was determined using the t-Distributed Stochastic Neighbor Embedding (t-SNE) algorithm. Each cluster is identified by a distinct color. Percent cellular abundance in each cluster is indicated. (e) Orthotopic KPC tumors were harvested from WT mice on days 7, 14, or 21 after tumor cell implantation and tumor-infiltrating CD45 + TCRβ + CD4 – CD8 – leukocytes were gated and tested for expression of NK1.1. Representative contour plots from days 7 and 21 and quantitative data comparing frequency of tumor-infiltrating iαβT per NKT cells at all time points are shown (n=5/time point). (f) Paraffin-embedded sections made from tumors of mice serially treated with anti-TCRγ/δ and NK1.1 depleting antibodies were tested for co-expression of Hematoxylin, CD3, CD4, and CD8 in the PDA TME. (g) CD45 + TCRβ + NK1.1 – leukocytes infiltrating orthotopic KPC tumors in WT and Fas lpr mice were gated and tested for expression of CD4 and CD8. Representative contour plots and quantitative data are shown (n=5/group). (h) The thymus from 6-month old WT and KC mice were harvested and CD45 + TCRβ + thymocytes were gated and tested for expression of CD4 and CD8. The frequency of iαβTs in the thymus was calculated (n=5/group). (i) CD4 + T cells, CD8 + T cells, or iαβTs were harvested from CD45.1 mice and transferred i.v. to orthotopic PDA-bearing CD45.2 mice. PDA tumors were harvested at 96 hours and CD45.1 + cells were gated and tested for CD4 and CD8 expression. Representative contour plots are shown (n=5/group). (j) WT mice were orthotopically administered KPC tumor cells and sacrificed on day 21. PDA-infiltrating CD4 + T cells, CD8 + T cells, and iαβTs were assayed for Ki67 proliferative index. Representative contour plots and quantitative data are shown (n=5). (k) Splenic and orthotopic PDA-infiltrating iαβTs were tested on day 21 for expression of CCR2, CCR5, and CCR6 (n=5). (l) The frequency of PDA-infiltrating iαβTs was tested on day 21 in WT, CCR2 –/– , CCR5 –/– , and CCR6 –/– hosts (n=5/group). All experiments were repeated at least 3 times (*p

    Techniques Used: Mouse Assay, Expressing, Multiplex Assay, Immunohistochemistry, Staining, Purification, FACS

    Related Articles

    Centrifugation:

    Article Title: Myocardial infarction stabilization by cell‐based expression of controlled Vascular Endothelial Growth Factor levels
    Article Snippet: .. Tissue was minced and digested with 0.15% collagenase (Worthington Biochemical Corporation, Lakewood, NJ) in phosphate‐buffered saline (PBS) at 37°C under continuous shaking for 60 min. After centrifugation at 1500 rpm for 10 min, the lipid‐rich layer was discarded and the cellular pellet was washed once with PBS. .. Released cells were strained through a 100‐μm nylon‐mesh followed by a 70‐μm nylon‐mesh to remove fibrous debris, plated at a density of 105 cells/cm2 and cultured in high‐glucose Dulbecco's modified Eagle's medium with 10% foetal bovine serum, as described .

    Homogenization:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

    Isolation:

    Article Title: Altered mitochondrial quality control in Atg7-deficient VSMCs promotes enhanced apoptosis and is linked to unstable atherosclerotic plaque phenotype
    Article Snippet: .. Briefly, isolated aortas were incubated with Hank's Balanced Salt Solution (HBSS) containing 1 mg/ml collagenase (Worthington, type II CLS, 4176), 1 mg/mL trypsin inhibitor aprotinin, 0.74 units/mL elastase (Worthington, 2279) and 1% penicillin/streptomycin for 15 min at 37 °C in 5% CO2 . ..

    Purification:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

    Concentration Assay:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

    Incubation:

    Article Title: Altered mitochondrial quality control in Atg7-deficient VSMCs promotes enhanced apoptosis and is linked to unstable atherosclerotic plaque phenotype
    Article Snippet: .. Briefly, isolated aortas were incubated with Hank's Balanced Salt Solution (HBSS) containing 1 mg/ml collagenase (Worthington, type II CLS, 4176), 1 mg/mL trypsin inhibitor aprotinin, 0.74 units/mL elastase (Worthington, 2279) and 1% penicillin/streptomycin for 15 min at 37 °C in 5% CO2 . ..

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    Worthington Biochemical avian ty 1
    Avian Ty 1, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian ty 1/product/Worthington Biochemical
    Average 85 stars, based on 1 article reviews
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