collagenase type ii  (Worthington Biochemical)


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    Name:
    Collagenase Type 2
    Description:
    Prepared to contain higher clostripain activity Suggested for bone heart liver thyroid and salivary primary cell isolation Supplied as a dialyzed lyophilized powder
    Catalog Number:
    LS004174
    Price:
    35
    Source:
    Clostridium histolyticum
    Size:
    100 mg
    Cas Number:
    9001.12.1
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    Structured Review

    Worthington Biochemical collagenase type ii
    Prepared to contain higher clostripain activity Suggested for bone heart liver thyroid and salivary primary cell isolation Supplied as a dialyzed lyophilized powder
    https://www.bioz.com/result/collagenase type ii/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase type ii - by Bioz Stars, 2021-10
    99/100 stars

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    Related Articles

    other:

    Article Title: Identification of candidate regulators of mandibular bone loss in FcγRIIB-/- Mice
    Article Snippet: Mandibles were minced and digested by collagenase type II (Worthington Biochemical Corporation, Lakewood, NJ, USA) for 2 h at 37 °C.

    Article Title: Self-organized emergence of hyaline cartilage in hiPSC-derived multi-tissue organoids
    Article Snippet: Organoid tissue retained on the filter was then back-flushed with 25 ml of cold HBSS and this tissue was further disaggregated in 2 ml Collagenase II (Celase GMP, Worthington Biochemical Corporation, Lakewood, NJ) also containing 200 μg DNAse1, for 5 minutes at 37°C.

    Article Title: Insulin signaling alters antioxidant capacity in the diabetic heart
    Article Snippet: Briefly, after isoflurane administration the heart was excised, the aorta was cannulated, and it was then perfused with type II collagenase (Worthington #LS004176).

    Article Title: Angiotensin-(1-7) protects against sepsis-associated left ventricular dysfunction induced by lipopolysaccharide
    Article Snippet: The hearts were excised and digested in phosphate buffer saline (PBS) containing collagenase type II (Worthington Biochemical Corp., NJ, USA) and pancreatin (Sigma).

    Incubation:

    Article Title: WNT16 is Robustly Increased by Oncostatin M in Mouse Calvarial Osteoblasts and Acts as a Negative Feedback Regulator of Osteoclast Formation Induced by Oncostatin M
    Article Snippet: .. Thereafter, the calvariae were incubated with 180 U/mL Collagenase type II (Worthington Biochemical Corporation, Lakewood, NJ, USA) in PBS for 10 sequential 10 min, 37°C, 600 rpm, 5 mL digestions. ..

    Isolation:

    Article Title: Interleukin-18 accelerates cardiac inflammation and dysfunction during ischemia/reperfusion injury by transcriptional activation of CXCL16
    Article Snippet: .. Then the hearts were dissected, LV tissues were isolated and minced into small fragments carefully, and digested gently in a solution containing collagenase-II (1.5 mg/mL, Worthington Biochemical Corporation), elastase (0.25 mg/ mL, Worthington Biochemical Corporation) and DNase I (0.5 mg/mL, Worthington Biochemical Corporation) for 45 min at 37 ◦C. ..

    Article Title: The oxidation‐resistant CaMKII‐MM281/282VV mutation does not prevent arrhythmias in CPVT1). The oxidation‐resistant CaMKII‐MM281/282VV mutation does not prevent arrhythmias in CPVT1
    Article Snippet: .. The perfusion solution was gradually switched to an isolation buffer containing 0.012 mM Ca2+ and ~500 U/mL of collagenase type II (Worthington). ..

    Article Title: Inhibition of cardiac PERK signaling promotes peripartum cardiac dysfunction
    Article Snippet: .. Briefly, the cardiacmyocytes were isolated from mouse hearts via coronary perfusion with collagenase type 2 (Worthington). ..

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  • 99
    Worthington Biochemical collagenase type ii
    Collagenase Type Ii, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase type ii/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase type ii - by Bioz Stars, 2021-10
    99/100 stars
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