a20 cells  (ATCC)


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    ATCC a20 cells
    A20 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a20 tib 208 cell lines  (ATCC)


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    ATCC a20 tib 208 cell lines
    A20 Tib 208 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a20 atcc atcc tib 208 oligonucleotides  (ATCC)


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    ATCC a20 atcc atcc tib 208 oligonucleotides
    A20 Atcc Atcc Tib 208 Oligonucleotides, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a20 b cell lymphoma tib 208 cells  (ATCC)


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    ATCC a20 b cell lymphoma tib 208 cells
    A20 B Cell Lymphoma Tib 208 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tib 208  (ATCC)


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    ATCC tib 208
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    a20 cells  (ATCC)


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    ATCC a20 cells
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    a20  (ATCC)


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    ATCC a20
    CD4+ T-cell epitopes of OVA and DBY display differential behavior towards H2-M. CD4+TCRvα2/β5+ T-cells derived from OT-2 mice were stained with CFSE and co-cultured with irradiated OVA peptide loaded 291PC (A, B) or <t>A20</t> (C) cells transduced with H2-M or H2-O. T-cell proliferation on day 5 is depicted as measured by flow cytometry. CD4+ TCRvβ6+ T-cells derived from Marilyn mice were stained with CFSE and co-cultured with DBY peptide loaded 291PC (D, E) or A20 (F) cell lines overexpressing H2-M or H2-O. T-cell proliferation on day 3 was measured as indicator for peptide presentation. Data are shown as mean±SEM of n=5–6 individually performed experiments. Panel A and D show representatives of six to seven independent experiments. A data summary of the six to seven individually performed experiments is shown in B and E. Statistical differences were calculated using paired t-test. *p<0.05; **p<0.01; ***p<0.001. ns, not significant; OVA, ovalbumin; WT, wildtype.
    A20, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Influence of DM-sensitivity on immunogenicity of MHC class II restricted antigens"

    Article Title: Influence of DM-sensitivity on immunogenicity of MHC class II restricted antigens

    Journal: Journal for Immunotherapy of Cancer

    doi: 10.1136/jitc-2021-002401

    CD4+ T-cell epitopes of OVA and DBY display differential behavior towards H2-M. CD4+TCRvα2/β5+ T-cells derived from OT-2 mice were stained with CFSE and co-cultured with irradiated OVA peptide loaded 291PC (A, B) or A20 (C) cells transduced with H2-M or H2-O. T-cell proliferation on day 5 is depicted as measured by flow cytometry. CD4+ TCRvβ6+ T-cells derived from Marilyn mice were stained with CFSE and co-cultured with DBY peptide loaded 291PC (D, E) or A20 (F) cell lines overexpressing H2-M or H2-O. T-cell proliferation on day 3 was measured as indicator for peptide presentation. Data are shown as mean±SEM of n=5–6 individually performed experiments. Panel A and D show representatives of six to seven independent experiments. A data summary of the six to seven individually performed experiments is shown in B and E. Statistical differences were calculated using paired t-test. *p<0.05; **p<0.01; ***p<0.001. ns, not significant; OVA, ovalbumin; WT, wildtype.
    Figure Legend Snippet: CD4+ T-cell epitopes of OVA and DBY display differential behavior towards H2-M. CD4+TCRvα2/β5+ T-cells derived from OT-2 mice were stained with CFSE and co-cultured with irradiated OVA peptide loaded 291PC (A, B) or A20 (C) cells transduced with H2-M or H2-O. T-cell proliferation on day 5 is depicted as measured by flow cytometry. CD4+ TCRvβ6+ T-cells derived from Marilyn mice were stained with CFSE and co-cultured with DBY peptide loaded 291PC (D, E) or A20 (F) cell lines overexpressing H2-M or H2-O. T-cell proliferation on day 3 was measured as indicator for peptide presentation. Data are shown as mean±SEM of n=5–6 individually performed experiments. Panel A and D show representatives of six to seven independent experiments. A data summary of the six to seven individually performed experiments is shown in B and E. Statistical differences were calculated using paired t-test. *p<0.05; **p<0.01; ***p<0.001. ns, not significant; OVA, ovalbumin; WT, wildtype.

    Techniques Used: Derivative Assay, Staining, Cell Culture, Irradiation, Transduction, Flow Cytometry

    OVA-derived OT-2 epitope can be switched from DM-sensitive to resistant. CD4+TCRvα2/β5+ T-cells derived from OT-2 mice were stained with CFSE and co-cultured with irradiated 291PC (A) or A20 (B) cells transduced with H2-M or H2-O and loaded with the indicated peptides. For (A) one representative of six independent experiments is shown. Proliferation was measured on day 5 using flow cytometry. (C) Depicted are proliferation data from panel B as ratio of H2-O/H2-M expressing cells as stimulators. Ratios >1 are defined as DM-sensitive, ratios <1 as DM-resistant. Illustrated are means±SEM (n=5). (D) Intracellular TNF production was measured after co-culture with A20 cells on day 3. (E) Shown are intracellular TNF data from panel D as ratio of H2-O/H2-M expressing cells as stimulators. Ratios >1 are defined as DM-sensitive, ratios <1 as DM-resistant. Illustrated are means±SEM (n=3). Statistical differences were calculated using paired t-test (B, D). Significance for the ratios in panel C and E was calculated using Kruskal-Wallis test for non-parametric data with multiple comparisons. *p<0.05; **p<0.01. ns, not significant; OVA, ovalbumin; TNF, tumor necrosis factor; WT, wildtype.
    Figure Legend Snippet: OVA-derived OT-2 epitope can be switched from DM-sensitive to resistant. CD4+TCRvα2/β5+ T-cells derived from OT-2 mice were stained with CFSE and co-cultured with irradiated 291PC (A) or A20 (B) cells transduced with H2-M or H2-O and loaded with the indicated peptides. For (A) one representative of six independent experiments is shown. Proliferation was measured on day 5 using flow cytometry. (C) Depicted are proliferation data from panel B as ratio of H2-O/H2-M expressing cells as stimulators. Ratios >1 are defined as DM-sensitive, ratios <1 as DM-resistant. Illustrated are means±SEM (n=5). (D) Intracellular TNF production was measured after co-culture with A20 cells on day 3. (E) Shown are intracellular TNF data from panel D as ratio of H2-O/H2-M expressing cells as stimulators. Ratios >1 are defined as DM-sensitive, ratios <1 as DM-resistant. Illustrated are means±SEM (n=3). Statistical differences were calculated using paired t-test (B, D). Significance for the ratios in panel C and E was calculated using Kruskal-Wallis test for non-parametric data with multiple comparisons. *p<0.05; **p<0.01. ns, not significant; OVA, ovalbumin; TNF, tumor necrosis factor; WT, wildtype.

    Techniques Used: Derivative Assay, Staining, Cell Culture, Irradiation, Transduction, Flow Cytometry, Expressing, Co-Culture Assay

    Mutation of DBY induces DM sensitivity. CD4+TCRvβ6+ T-cells derived from Marilyn mice were stained with CFSE and cocultured with irradiated 291PC (A) or A20 (B) cells transduced with H2-M or H2-O and loaded with the indicated peptides. Proliferation was measured on day 3 using flow cytometry. For (A) one representative of six independent experiments is shown. (C) Depicted are proliferation data from panel B as ratio of H2-O/H2-M expressing cells as stimulators. Ratios >1 are defined as DM-sensitive, ratios <1 as DM-resistant. Means±SEM are indicated (n≥3). (D) IFN-γ secretion was measured by ELISA after co-culture with A20 cells by day 2. (E) Shown are IFN-γ data from panel D as ratio of H2-O/H2-M expressing cells as stimulators. Ratios >1 are defined as DM-sensitive, ratios <1 as DM-resistant. Demonstrated are means±SEM (n≥2). Statistical differences were calculated using paired t-test (B, D). Significance for the ratios in panel C and E was calculated using Kruskal-Wallis test for non-parametric data with multiple comparisons. *p<0.05; **p<0.01; ***p<0.001. IFN, interferon; ns, not significant; WT, wildtype.
    Figure Legend Snippet: Mutation of DBY induces DM sensitivity. CD4+TCRvβ6+ T-cells derived from Marilyn mice were stained with CFSE and cocultured with irradiated 291PC (A) or A20 (B) cells transduced with H2-M or H2-O and loaded with the indicated peptides. Proliferation was measured on day 3 using flow cytometry. For (A) one representative of six independent experiments is shown. (C) Depicted are proliferation data from panel B as ratio of H2-O/H2-M expressing cells as stimulators. Ratios >1 are defined as DM-sensitive, ratios <1 as DM-resistant. Means±SEM are indicated (n≥3). (D) IFN-γ secretion was measured by ELISA after co-culture with A20 cells by day 2. (E) Shown are IFN-γ data from panel D as ratio of H2-O/H2-M expressing cells as stimulators. Ratios >1 are defined as DM-sensitive, ratios <1 as DM-resistant. Demonstrated are means±SEM (n≥2). Statistical differences were calculated using paired t-test (B, D). Significance for the ratios in panel C and E was calculated using Kruskal-Wallis test for non-parametric data with multiple comparisons. *p<0.05; **p<0.01; ***p<0.001. IFN, interferon; ns, not significant; WT, wildtype.

    Techniques Used: Mutagenesis, Derivative Assay, Staining, Irradiation, Transduction, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Co-Culture Assay

    a20 cells  (ATCC)


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    ATCC a20 cells
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    murine b cell lymphoma line a20  (ATCC)


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    ATCC murine b cell lymphoma line a20
    Murine B Cell Lymphoma Line A20, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse lymphoma cell line a20 cells  (ATCC)


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    ATCC mouse lymphoma cell line a20 cells
    Effect of drug serum containing JDX on the proliferation, migration, and invasiveness of <t>A20</t> cells. (a) The cell viability as measured by CCK8 assay in control, co-culture with HSCs, and drug serum treated group. (b) Measurement of cell migration as detected by Transwell assay in the above three groups. (c) The numbers of migration cells in the three groups. (d) Detection of cell invasion as detected by Transwell assay in the above three groups. (e) The numbers of invasion cells in the three groups. The control group was treated with control serum. The co-culture with HSCs group was treated with control serum in the co-culture system of A20 cells and HSCs. The drug serum group was treated with serum containing JDX in the co-culture system of A20 cells and HSCs. ∗ Control group vs co-culture with HSCs group and drug serum group. # Drug serum group vs co-culture with HSCs group. ∗ or # P < 0.05; ∗∗ or ## P < 0.01.
    Mouse Lymphoma Cell Line A20 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The Combination of Jiedu Xiaoluo Decoction with Autologous Peripheral Blood Stem Cell Transplantation (APBSCT) Accelerates Disease Remission of Non-Hodgkin Lymphoma"

    Article Title: The Combination of Jiedu Xiaoluo Decoction with Autologous Peripheral Blood Stem Cell Transplantation (APBSCT) Accelerates Disease Remission of Non-Hodgkin Lymphoma

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2021/2745705

    Effect of drug serum containing JDX on the proliferation, migration, and invasiveness of A20 cells. (a) The cell viability as measured by CCK8 assay in control, co-culture with HSCs, and drug serum treated group. (b) Measurement of cell migration as detected by Transwell assay in the above three groups. (c) The numbers of migration cells in the three groups. (d) Detection of cell invasion as detected by Transwell assay in the above three groups. (e) The numbers of invasion cells in the three groups. The control group was treated with control serum. The co-culture with HSCs group was treated with control serum in the co-culture system of A20 cells and HSCs. The drug serum group was treated with serum containing JDX in the co-culture system of A20 cells and HSCs. ∗ Control group vs co-culture with HSCs group and drug serum group. # Drug serum group vs co-culture with HSCs group. ∗ or # P < 0.05; ∗∗ or ## P < 0.01.
    Figure Legend Snippet: Effect of drug serum containing JDX on the proliferation, migration, and invasiveness of A20 cells. (a) The cell viability as measured by CCK8 assay in control, co-culture with HSCs, and drug serum treated group. (b) Measurement of cell migration as detected by Transwell assay in the above three groups. (c) The numbers of migration cells in the three groups. (d) Detection of cell invasion as detected by Transwell assay in the above three groups. (e) The numbers of invasion cells in the three groups. The control group was treated with control serum. The co-culture with HSCs group was treated with control serum in the co-culture system of A20 cells and HSCs. The drug serum group was treated with serum containing JDX in the co-culture system of A20 cells and HSCs. ∗ Control group vs co-culture with HSCs group and drug serum group. # Drug serum group vs co-culture with HSCs group. ∗ or # P < 0.05; ∗∗ or ## P < 0.01.

    Techniques Used: Migration, CCK-8 Assay, Co-Culture Assay, Transwell Assay

    Effect of drug serum containing JDX on HSCs-mediated cell apoptosis of A20 cells. (a) The representative image of apoptosis cells in control, co-culture with HSCs, and drug serum treated group as measured by flow cytometry. (b) The statistical analyses of the apoptotic cell rate in the above three groups.
    Figure Legend Snippet: Effect of drug serum containing JDX on HSCs-mediated cell apoptosis of A20 cells. (a) The representative image of apoptosis cells in control, co-culture with HSCs, and drug serum treated group as measured by flow cytometry. (b) The statistical analyses of the apoptotic cell rate in the above three groups.

    Techniques Used: Co-Culture Assay, Flow Cytometry

    The mRNA and protein levels of inflammatory cytokines in A20 cells. (a) Transcriptional level of IL-10 (a), IL-12 (b), IFN- γ (c), TGF- β (d), and VEGF (e) as determined by qRT-PCR in control, co-culture with HSCs, and drug serum treated group. Cells were treated at 24 h, 48 h, and 72 h, respectively. (f) The protein levels of IL-10, IL-12, IFN- γ , TGF- β , and VEGF as detected by western blotting. GAPDH served as the internal control. ∗ Control group vs co-culture with HSCs group and drug serum group. # Drug serum group vs co-culture with HSCs group. ∗ or # P < 0.05; ∗∗ or ## P < 0.01.
    Figure Legend Snippet: The mRNA and protein levels of inflammatory cytokines in A20 cells. (a) Transcriptional level of IL-10 (a), IL-12 (b), IFN- γ (c), TGF- β (d), and VEGF (e) as determined by qRT-PCR in control, co-culture with HSCs, and drug serum treated group. Cells were treated at 24 h, 48 h, and 72 h, respectively. (f) The protein levels of IL-10, IL-12, IFN- γ , TGF- β , and VEGF as detected by western blotting. GAPDH served as the internal control. ∗ Control group vs co-culture with HSCs group and drug serum group. # Drug serum group vs co-culture with HSCs group. ∗ or # P < 0.05; ∗∗ or ## P < 0.01.

    Techniques Used: Quantitative RT-PCR, Co-Culture Assay, Western Blot

    a20 cells  (ATCC)


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    ATCC a20 cells
    A20 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a20  (ATCC)
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    ATCC a20
    CD4+ T-cell epitopes of OVA and DBY display differential behavior towards H2-M. CD4+TCRvα2/β5+ T-cells derived from OT-2 mice were stained with CFSE and co-cultured with irradiated OVA peptide loaded 291PC (A, B) or <t>A20</t> (C) cells transduced with H2-M or H2-O. T-cell proliferation on day 5 is depicted as measured by flow cytometry. CD4+ TCRvβ6+ T-cells derived from Marilyn mice were stained with CFSE and co-cultured with DBY peptide loaded 291PC (D, E) or A20 (F) cell lines overexpressing H2-M or H2-O. T-cell proliferation on day 3 was measured as indicator for peptide presentation. Data are shown as mean±SEM of n=5–6 individually performed experiments. Panel A and D show representatives of six to seven independent experiments. A data summary of the six to seven individually performed experiments is shown in B and E. Statistical differences were calculated using paired t-test. *p<0.05; **p<0.01; ***p<0.001. ns, not significant; OVA, ovalbumin; WT, wildtype.
    A20, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC murine b cell lymphoma line a20
    CD4+ T-cell epitopes of OVA and DBY display differential behavior towards H2-M. CD4+TCRvα2/β5+ T-cells derived from OT-2 mice were stained with CFSE and co-cultured with irradiated OVA peptide loaded 291PC (A, B) or <t>A20</t> (C) cells transduced with H2-M or H2-O. T-cell proliferation on day 5 is depicted as measured by flow cytometry. CD4+ TCRvβ6+ T-cells derived from Marilyn mice were stained with CFSE and co-cultured with DBY peptide loaded 291PC (D, E) or A20 (F) cell lines overexpressing H2-M or H2-O. T-cell proliferation on day 3 was measured as indicator for peptide presentation. Data are shown as mean±SEM of n=5–6 individually performed experiments. Panel A and D show representatives of six to seven independent experiments. A data summary of the six to seven individually performed experiments is shown in B and E. Statistical differences were calculated using paired t-test. *p<0.05; **p<0.01; ***p<0.001. ns, not significant; OVA, ovalbumin; WT, wildtype.
    Murine B Cell Lymphoma Line A20, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC mouse lymphoma cell line a20 cells
    Effect of drug serum containing JDX on the proliferation, migration, and invasiveness of <t>A20</t> cells. (a) The cell viability as measured by CCK8 assay in control, co-culture with HSCs, and drug serum treated group. (b) Measurement of cell migration as detected by Transwell assay in the above three groups. (c) The numbers of migration cells in the three groups. (d) Detection of cell invasion as detected by Transwell assay in the above three groups. (e) The numbers of invasion cells in the three groups. The control group was treated with control serum. The co-culture with HSCs group was treated with control serum in the co-culture system of A20 cells and HSCs. The drug serum group was treated with serum containing JDX in the co-culture system of A20 cells and HSCs. ∗ Control group vs co-culture with HSCs group and drug serum group. # Drug serum group vs co-culture with HSCs group. ∗ or # P < 0.05; ∗∗ or ## P < 0.01.
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    CD4+ T-cell epitopes of OVA and DBY display differential behavior towards H2-M. CD4+TCRvα2/β5+ T-cells derived from OT-2 mice were stained with CFSE and co-cultured with irradiated OVA peptide loaded 291PC (A, B) or A20 (C) cells transduced with H2-M or H2-O. T-cell proliferation on day 5 is depicted as measured by flow cytometry. CD4+ TCRvβ6+ T-cells derived from Marilyn mice were stained with CFSE and co-cultured with DBY peptide loaded 291PC (D, E) or A20 (F) cell lines overexpressing H2-M or H2-O. T-cell proliferation on day 3 was measured as indicator for peptide presentation. Data are shown as mean±SEM of n=5–6 individually performed experiments. Panel A and D show representatives of six to seven independent experiments. A data summary of the six to seven individually performed experiments is shown in B and E. Statistical differences were calculated using paired t-test. *p<0.05; **p<0.01; ***p<0.001. ns, not significant; OVA, ovalbumin; WT, wildtype.

    Journal: Journal for Immunotherapy of Cancer

    Article Title: Influence of DM-sensitivity on immunogenicity of MHC class II restricted antigens

    doi: 10.1136/jitc-2021-002401

    Figure Lengend Snippet: CD4+ T-cell epitopes of OVA and DBY display differential behavior towards H2-M. CD4+TCRvα2/β5+ T-cells derived from OT-2 mice were stained with CFSE and co-cultured with irradiated OVA peptide loaded 291PC (A, B) or A20 (C) cells transduced with H2-M or H2-O. T-cell proliferation on day 5 is depicted as measured by flow cytometry. CD4+ TCRvβ6+ T-cells derived from Marilyn mice were stained with CFSE and co-cultured with DBY peptide loaded 291PC (D, E) or A20 (F) cell lines overexpressing H2-M or H2-O. T-cell proliferation on day 3 was measured as indicator for peptide presentation. Data are shown as mean±SEM of n=5–6 individually performed experiments. Panel A and D show representatives of six to seven independent experiments. A data summary of the six to seven individually performed experiments is shown in B and E. Statistical differences were calculated using paired t-test. *p<0.05; **p<0.01; ***p<0.001. ns, not significant; OVA, ovalbumin; WT, wildtype.

    Article Snippet: 291PC, A20 (ATCC, TIB-208), Phoenix-A (ATCC, CRL-3213), and EBV-LCL were cultured in RPMI 1640 (PAN-Biotech) with 10% FCS, 40 IU/mL penicillin, 40 µg/mL streptomycin, 2 mM l-glutamine, 0.4% vitamin solution, 50 µM β-mercaptoethanol, 1% minimal essential media, and 1 mM sodium pyruvate (all Gibco, Thermo Fisher Scientific).

    Techniques: Derivative Assay, Staining, Cell Culture, Irradiation, Transduction, Flow Cytometry

    OVA-derived OT-2 epitope can be switched from DM-sensitive to resistant. CD4+TCRvα2/β5+ T-cells derived from OT-2 mice were stained with CFSE and co-cultured with irradiated 291PC (A) or A20 (B) cells transduced with H2-M or H2-O and loaded with the indicated peptides. For (A) one representative of six independent experiments is shown. Proliferation was measured on day 5 using flow cytometry. (C) Depicted are proliferation data from panel B as ratio of H2-O/H2-M expressing cells as stimulators. Ratios >1 are defined as DM-sensitive, ratios <1 as DM-resistant. Illustrated are means±SEM (n=5). (D) Intracellular TNF production was measured after co-culture with A20 cells on day 3. (E) Shown are intracellular TNF data from panel D as ratio of H2-O/H2-M expressing cells as stimulators. Ratios >1 are defined as DM-sensitive, ratios <1 as DM-resistant. Illustrated are means±SEM (n=3). Statistical differences were calculated using paired t-test (B, D). Significance for the ratios in panel C and E was calculated using Kruskal-Wallis test for non-parametric data with multiple comparisons. *p<0.05; **p<0.01. ns, not significant; OVA, ovalbumin; TNF, tumor necrosis factor; WT, wildtype.

    Journal: Journal for Immunotherapy of Cancer

    Article Title: Influence of DM-sensitivity on immunogenicity of MHC class II restricted antigens

    doi: 10.1136/jitc-2021-002401

    Figure Lengend Snippet: OVA-derived OT-2 epitope can be switched from DM-sensitive to resistant. CD4+TCRvα2/β5+ T-cells derived from OT-2 mice were stained with CFSE and co-cultured with irradiated 291PC (A) or A20 (B) cells transduced with H2-M or H2-O and loaded with the indicated peptides. For (A) one representative of six independent experiments is shown. Proliferation was measured on day 5 using flow cytometry. (C) Depicted are proliferation data from panel B as ratio of H2-O/H2-M expressing cells as stimulators. Ratios >1 are defined as DM-sensitive, ratios <1 as DM-resistant. Illustrated are means±SEM (n=5). (D) Intracellular TNF production was measured after co-culture with A20 cells on day 3. (E) Shown are intracellular TNF data from panel D as ratio of H2-O/H2-M expressing cells as stimulators. Ratios >1 are defined as DM-sensitive, ratios <1 as DM-resistant. Illustrated are means±SEM (n=3). Statistical differences were calculated using paired t-test (B, D). Significance for the ratios in panel C and E was calculated using Kruskal-Wallis test for non-parametric data with multiple comparisons. *p<0.05; **p<0.01. ns, not significant; OVA, ovalbumin; TNF, tumor necrosis factor; WT, wildtype.

    Article Snippet: 291PC, A20 (ATCC, TIB-208), Phoenix-A (ATCC, CRL-3213), and EBV-LCL were cultured in RPMI 1640 (PAN-Biotech) with 10% FCS, 40 IU/mL penicillin, 40 µg/mL streptomycin, 2 mM l-glutamine, 0.4% vitamin solution, 50 µM β-mercaptoethanol, 1% minimal essential media, and 1 mM sodium pyruvate (all Gibco, Thermo Fisher Scientific).

    Techniques: Derivative Assay, Staining, Cell Culture, Irradiation, Transduction, Flow Cytometry, Expressing, Co-Culture Assay

    Mutation of DBY induces DM sensitivity. CD4+TCRvβ6+ T-cells derived from Marilyn mice were stained with CFSE and cocultured with irradiated 291PC (A) or A20 (B) cells transduced with H2-M or H2-O and loaded with the indicated peptides. Proliferation was measured on day 3 using flow cytometry. For (A) one representative of six independent experiments is shown. (C) Depicted are proliferation data from panel B as ratio of H2-O/H2-M expressing cells as stimulators. Ratios >1 are defined as DM-sensitive, ratios <1 as DM-resistant. Means±SEM are indicated (n≥3). (D) IFN-γ secretion was measured by ELISA after co-culture with A20 cells by day 2. (E) Shown are IFN-γ data from panel D as ratio of H2-O/H2-M expressing cells as stimulators. Ratios >1 are defined as DM-sensitive, ratios <1 as DM-resistant. Demonstrated are means±SEM (n≥2). Statistical differences were calculated using paired t-test (B, D). Significance for the ratios in panel C and E was calculated using Kruskal-Wallis test for non-parametric data with multiple comparisons. *p<0.05; **p<0.01; ***p<0.001. IFN, interferon; ns, not significant; WT, wildtype.

    Journal: Journal for Immunotherapy of Cancer

    Article Title: Influence of DM-sensitivity on immunogenicity of MHC class II restricted antigens

    doi: 10.1136/jitc-2021-002401

    Figure Lengend Snippet: Mutation of DBY induces DM sensitivity. CD4+TCRvβ6+ T-cells derived from Marilyn mice were stained with CFSE and cocultured with irradiated 291PC (A) or A20 (B) cells transduced with H2-M or H2-O and loaded with the indicated peptides. Proliferation was measured on day 3 using flow cytometry. For (A) one representative of six independent experiments is shown. (C) Depicted are proliferation data from panel B as ratio of H2-O/H2-M expressing cells as stimulators. Ratios >1 are defined as DM-sensitive, ratios <1 as DM-resistant. Means±SEM are indicated (n≥3). (D) IFN-γ secretion was measured by ELISA after co-culture with A20 cells by day 2. (E) Shown are IFN-γ data from panel D as ratio of H2-O/H2-M expressing cells as stimulators. Ratios >1 are defined as DM-sensitive, ratios <1 as DM-resistant. Demonstrated are means±SEM (n≥2). Statistical differences were calculated using paired t-test (B, D). Significance for the ratios in panel C and E was calculated using Kruskal-Wallis test for non-parametric data with multiple comparisons. *p<0.05; **p<0.01; ***p<0.001. IFN, interferon; ns, not significant; WT, wildtype.

    Article Snippet: 291PC, A20 (ATCC, TIB-208), Phoenix-A (ATCC, CRL-3213), and EBV-LCL were cultured in RPMI 1640 (PAN-Biotech) with 10% FCS, 40 IU/mL penicillin, 40 µg/mL streptomycin, 2 mM l-glutamine, 0.4% vitamin solution, 50 µM β-mercaptoethanol, 1% minimal essential media, and 1 mM sodium pyruvate (all Gibco, Thermo Fisher Scientific).

    Techniques: Mutagenesis, Derivative Assay, Staining, Irradiation, Transduction, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Co-Culture Assay

    Effect of drug serum containing JDX on the proliferation, migration, and invasiveness of A20 cells. (a) The cell viability as measured by CCK8 assay in control, co-culture with HSCs, and drug serum treated group. (b) Measurement of cell migration as detected by Transwell assay in the above three groups. (c) The numbers of migration cells in the three groups. (d) Detection of cell invasion as detected by Transwell assay in the above three groups. (e) The numbers of invasion cells in the three groups. The control group was treated with control serum. The co-culture with HSCs group was treated with control serum in the co-culture system of A20 cells and HSCs. The drug serum group was treated with serum containing JDX in the co-culture system of A20 cells and HSCs. ∗ Control group vs co-culture with HSCs group and drug serum group. # Drug serum group vs co-culture with HSCs group. ∗ or # P < 0.05; ∗∗ or ## P < 0.01.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: The Combination of Jiedu Xiaoluo Decoction with Autologous Peripheral Blood Stem Cell Transplantation (APBSCT) Accelerates Disease Remission of Non-Hodgkin Lymphoma

    doi: 10.1155/2021/2745705

    Figure Lengend Snippet: Effect of drug serum containing JDX on the proliferation, migration, and invasiveness of A20 cells. (a) The cell viability as measured by CCK8 assay in control, co-culture with HSCs, and drug serum treated group. (b) Measurement of cell migration as detected by Transwell assay in the above three groups. (c) The numbers of migration cells in the three groups. (d) Detection of cell invasion as detected by Transwell assay in the above three groups. (e) The numbers of invasion cells in the three groups. The control group was treated with control serum. The co-culture with HSCs group was treated with control serum in the co-culture system of A20 cells and HSCs. The drug serum group was treated with serum containing JDX in the co-culture system of A20 cells and HSCs. ∗ Control group vs co-culture with HSCs group and drug serum group. # Drug serum group vs co-culture with HSCs group. ∗ or # P < 0.05; ∗∗ or ## P < 0.01.

    Article Snippet: Mouse lymphoma cell line A20 cells were purchased from American Type Culture Collection (ATCC).

    Techniques: Migration, CCK-8 Assay, Co-Culture Assay, Transwell Assay

    Effect of drug serum containing JDX on HSCs-mediated cell apoptosis of A20 cells. (a) The representative image of apoptosis cells in control, co-culture with HSCs, and drug serum treated group as measured by flow cytometry. (b) The statistical analyses of the apoptotic cell rate in the above three groups.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: The Combination of Jiedu Xiaoluo Decoction with Autologous Peripheral Blood Stem Cell Transplantation (APBSCT) Accelerates Disease Remission of Non-Hodgkin Lymphoma

    doi: 10.1155/2021/2745705

    Figure Lengend Snippet: Effect of drug serum containing JDX on HSCs-mediated cell apoptosis of A20 cells. (a) The representative image of apoptosis cells in control, co-culture with HSCs, and drug serum treated group as measured by flow cytometry. (b) The statistical analyses of the apoptotic cell rate in the above three groups.

    Article Snippet: Mouse lymphoma cell line A20 cells were purchased from American Type Culture Collection (ATCC).

    Techniques: Co-Culture Assay, Flow Cytometry

    The mRNA and protein levels of inflammatory cytokines in A20 cells. (a) Transcriptional level of IL-10 (a), IL-12 (b), IFN- γ (c), TGF- β (d), and VEGF (e) as determined by qRT-PCR in control, co-culture with HSCs, and drug serum treated group. Cells were treated at 24 h, 48 h, and 72 h, respectively. (f) The protein levels of IL-10, IL-12, IFN- γ , TGF- β , and VEGF as detected by western blotting. GAPDH served as the internal control. ∗ Control group vs co-culture with HSCs group and drug serum group. # Drug serum group vs co-culture with HSCs group. ∗ or # P < 0.05; ∗∗ or ## P < 0.01.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: The Combination of Jiedu Xiaoluo Decoction with Autologous Peripheral Blood Stem Cell Transplantation (APBSCT) Accelerates Disease Remission of Non-Hodgkin Lymphoma

    doi: 10.1155/2021/2745705

    Figure Lengend Snippet: The mRNA and protein levels of inflammatory cytokines in A20 cells. (a) Transcriptional level of IL-10 (a), IL-12 (b), IFN- γ (c), TGF- β (d), and VEGF (e) as determined by qRT-PCR in control, co-culture with HSCs, and drug serum treated group. Cells were treated at 24 h, 48 h, and 72 h, respectively. (f) The protein levels of IL-10, IL-12, IFN- γ , TGF- β , and VEGF as detected by western blotting. GAPDH served as the internal control. ∗ Control group vs co-culture with HSCs group and drug serum group. # Drug serum group vs co-culture with HSCs group. ∗ or # P < 0.05; ∗∗ or ## P < 0.01.

    Article Snippet: Mouse lymphoma cell line A20 cells were purchased from American Type Culture Collection (ATCC).

    Techniques: Quantitative RT-PCR, Co-Culture Assay, Western Blot