reporter cell line thp1 blue nf κb  (ATCC)


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    Structured Review

    ATCC reporter cell line thp1 blue nf κb
    NF-κB activation in <t>THP1-Blue™</t> NF-κB reporter cells after C. ulcerans infection. THP1-Blue™ NF-κB cells were incubated for 20 h with ( A ) viable and ( B ) UV-killed bacteria of the non-pathogenic C. glutamicum
    Reporter Cell Line Thp1 Blue Nf κb, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reporter cell line thp1 blue nf κb/product/ATCC
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    reporter cell line thp1 blue nf κb - by Bioz Stars, 2022-10
    93/100 stars

    Images

    1) Product Images from "The killing of macrophages by Corynebacterium ulcerans"

    Article Title: The killing of macrophages by Corynebacterium ulcerans

    Journal: Virulence

    doi: 10.1080/21505594.2015.1125068

    NF-κB activation in THP1-Blue™ NF-κB reporter cells after C. ulcerans infection. THP1-Blue™ NF-κB cells were incubated for 20 h with ( A ) viable and ( B ) UV-killed bacteria of the non-pathogenic C. glutamicum
    Figure Legend Snippet: NF-κB activation in THP1-Blue™ NF-κB reporter cells after C. ulcerans infection. THP1-Blue™ NF-κB cells were incubated for 20 h with ( A ) viable and ( B ) UV-killed bacteria of the non-pathogenic C. glutamicum

    Techniques Used: Activation Assay, Infection, Incubation

    LDH release of THP1-Blue cells infected with C. ulcerans . The release of lactate dehydrogenase (LDH) as a sign of host cell damage during infection of THP1-Blue™ NF-κB cells with C. ulcerans was measured using the cytotoxicity detection
    Figure Legend Snippet: LDH release of THP1-Blue cells infected with C. ulcerans . The release of lactate dehydrogenase (LDH) as a sign of host cell damage during infection of THP1-Blue™ NF-κB cells with C. ulcerans was measured using the cytotoxicity detection

    Techniques Used: Infection

    2) Product Images from "Different Concentrations of Lactobacillus acidophilus Cell Free Filtrate Have Differing Anti-Biofilm and Immunomodulatory Effects"

    Article Title: Different Concentrations of Lactobacillus acidophilus Cell Free Filtrate Have Differing Anti-Biofilm and Immunomodulatory Effects

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2021.737392

    L. acidophilus cell free filtrate (CFF) has concentration-dependent effects on TNFα secretion in THP-1 monocytes and monocyte derived macrophages. THP-1 monocytes or monocyte derived macrophages were incubated with L. acidophilus 10 8 or 10 6 CFF alone or with cells 1 h after LPS stimulation. Supernatants were obtained 20 h after incubation and analyzed for levels of TNFα using ELISA. (A) L. acidophilus 10 8 CFF increased TNFα secretion in LPS-stimulated THP-1 monocytes, but 10 6 CFF had no effect. (B) In THP-1 monocyte derived macrophages, L. acidophilus 10 8 CFF alone promoted TNFα production while 10 6 CFF did not significantly alter TNFα production. (C) When THP-1 monocyte derived macrophages were stimulated with 50 ng/mL LPS 1 h prior to L. acidophilus CFF treatment, 10 6 CFF significantly attenuated TNFα production. Data are mean ± s.e.m. % of LPS Control data are mean ± s.e.m. percent change from LPS control adjusted to 100%. *p
    Figure Legend Snippet: L. acidophilus cell free filtrate (CFF) has concentration-dependent effects on TNFα secretion in THP-1 monocytes and monocyte derived macrophages. THP-1 monocytes or monocyte derived macrophages were incubated with L. acidophilus 10 8 or 10 6 CFF alone or with cells 1 h after LPS stimulation. Supernatants were obtained 20 h after incubation and analyzed for levels of TNFα using ELISA. (A) L. acidophilus 10 8 CFF increased TNFα secretion in LPS-stimulated THP-1 monocytes, but 10 6 CFF had no effect. (B) In THP-1 monocyte derived macrophages, L. acidophilus 10 8 CFF alone promoted TNFα production while 10 6 CFF did not significantly alter TNFα production. (C) When THP-1 monocyte derived macrophages were stimulated with 50 ng/mL LPS 1 h prior to L. acidophilus CFF treatment, 10 6 CFF significantly attenuated TNFα production. Data are mean ± s.e.m. % of LPS Control data are mean ± s.e.m. percent change from LPS control adjusted to 100%. *p

    Techniques Used: Concentration Assay, Derivative Assay, Incubation, Enzyme-linked Immunosorbent Assay

    L. acidophilus cell free filtrate (CFF) affects NF-κB activity. Using a luciferase NF-κB reporter THP-1 cell line, monocytes and monocyte derived macrophages were incubated with L. acidophilus CFF alone or added 1 h after LPS stimulation, for 20 h. Luciferase luminescence was measured to quantify NF-κB activation. (A) In monocytes, 10 8 CFF alone upregulated NF-κB activation but 10 6 CFF had no effect. (B) In LPS stimulated monocytes, only 10 6 CFF reduced NF-κB activity. (C) L. acidophilus 10 8 CFF increased NF-κB activity in monocyte derived macrophages. (D) Similar to monocytes, only L. acidophilus 10 6 CFF significantly reduced NF-κB activity in monocyte derived macrophages. Data are relative light units (RLUs) or mean ± s.e.m. percent change from LPS control adjusted to 100%. **p
    Figure Legend Snippet: L. acidophilus cell free filtrate (CFF) affects NF-κB activity. Using a luciferase NF-κB reporter THP-1 cell line, monocytes and monocyte derived macrophages were incubated with L. acidophilus CFF alone or added 1 h after LPS stimulation, for 20 h. Luciferase luminescence was measured to quantify NF-κB activation. (A) In monocytes, 10 8 CFF alone upregulated NF-κB activation but 10 6 CFF had no effect. (B) In LPS stimulated monocytes, only 10 6 CFF reduced NF-κB activity. (C) L. acidophilus 10 8 CFF increased NF-κB activity in monocyte derived macrophages. (D) Similar to monocytes, only L. acidophilus 10 6 CFF significantly reduced NF-κB activity in monocyte derived macrophages. Data are relative light units (RLUs) or mean ± s.e.m. percent change from LPS control adjusted to 100%. **p

    Techniques Used: Activity Assay, Luciferase, Derivative Assay, Incubation, Activation Assay

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    ATCC reporter cell line thp1 blue nf κb
    NF-κB activation in <t>THP1-Blue™</t> NF-κB reporter cells after C. ulcerans infection. THP1-Blue™ NF-κB cells were incubated for 20 h with ( A ) viable and ( B ) UV-killed bacteria of the non-pathogenic C. glutamicum
    Reporter Cell Line Thp1 Blue Nf κb, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reporter cell line thp1 blue nf κb/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    reporter cell line thp1 blue nf κb - by Bioz Stars, 2022-10
    93/100 stars
      Buy from Supplier

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    NF-κB activation in THP1-Blue™ NF-κB reporter cells after C. ulcerans infection. THP1-Blue™ NF-κB cells were incubated for 20 h with ( A ) viable and ( B ) UV-killed bacteria of the non-pathogenic C. glutamicum

    Journal: Virulence

    Article Title: The killing of macrophages by Corynebacterium ulcerans

    doi: 10.1080/21505594.2015.1125068

    Figure Lengend Snippet: NF-κB activation in THP1-Blue™ NF-κB reporter cells after C. ulcerans infection. THP1-Blue™ NF-κB cells were incubated for 20 h with ( A ) viable and ( B ) UV-killed bacteria of the non-pathogenic C. glutamicum

    Article Snippet: Cells of the reporter cell line THP1-Blue NF-κB were incubated for 20 hours with viable ( ) and UV-killed ( ) bacteria of non-pathogenic C. glutamicum ATCC 13032 and pathogenic C. ulcerans strains 809 and BR-AD22.

    Techniques: Activation Assay, Infection, Incubation

    LDH release of THP1-Blue cells infected with C. ulcerans . The release of lactate dehydrogenase (LDH) as a sign of host cell damage during infection of THP1-Blue™ NF-κB cells with C. ulcerans was measured using the cytotoxicity detection

    Journal: Virulence

    Article Title: The killing of macrophages by Corynebacterium ulcerans

    doi: 10.1080/21505594.2015.1125068

    Figure Lengend Snippet: LDH release of THP1-Blue cells infected with C. ulcerans . The release of lactate dehydrogenase (LDH) as a sign of host cell damage during infection of THP1-Blue™ NF-κB cells with C. ulcerans was measured using the cytotoxicity detection

    Article Snippet: Cells of the reporter cell line THP1-Blue NF-κB were incubated for 20 hours with viable ( ) and UV-killed ( ) bacteria of non-pathogenic C. glutamicum ATCC 13032 and pathogenic C. ulcerans strains 809 and BR-AD22.

    Techniques: Infection

    L. acidophilus cell free filtrate (CFF) has concentration-dependent effects on TNFα secretion in THP-1 monocytes and monocyte derived macrophages. THP-1 monocytes or monocyte derived macrophages were incubated with L. acidophilus 10 8 or 10 6 CFF alone or with cells 1 h after LPS stimulation. Supernatants were obtained 20 h after incubation and analyzed for levels of TNFα using ELISA. (A) L. acidophilus 10 8 CFF increased TNFα secretion in LPS-stimulated THP-1 monocytes, but 10 6 CFF had no effect. (B) In THP-1 monocyte derived macrophages, L. acidophilus 10 8 CFF alone promoted TNFα production while 10 6 CFF did not significantly alter TNFα production. (C) When THP-1 monocyte derived macrophages were stimulated with 50 ng/mL LPS 1 h prior to L. acidophilus CFF treatment, 10 6 CFF significantly attenuated TNFα production. Data are mean ± s.e.m. % of LPS Control data are mean ± s.e.m. percent change from LPS control adjusted to 100%. *p

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Different Concentrations of Lactobacillus acidophilus Cell Free Filtrate Have Differing Anti-Biofilm and Immunomodulatory Effects

    doi: 10.3389/fcimb.2021.737392

    Figure Lengend Snippet: L. acidophilus cell free filtrate (CFF) has concentration-dependent effects on TNFα secretion in THP-1 monocytes and monocyte derived macrophages. THP-1 monocytes or monocyte derived macrophages were incubated with L. acidophilus 10 8 or 10 6 CFF alone or with cells 1 h after LPS stimulation. Supernatants were obtained 20 h after incubation and analyzed for levels of TNFα using ELISA. (A) L. acidophilus 10 8 CFF increased TNFα secretion in LPS-stimulated THP-1 monocytes, but 10 6 CFF had no effect. (B) In THP-1 monocyte derived macrophages, L. acidophilus 10 8 CFF alone promoted TNFα production while 10 6 CFF did not significantly alter TNFα production. (C) When THP-1 monocyte derived macrophages were stimulated with 50 ng/mL LPS 1 h prior to L. acidophilus CFF treatment, 10 6 CFF significantly attenuated TNFα production. Data are mean ± s.e.m. % of LPS Control data are mean ± s.e.m. percent change from LPS control adjusted to 100%. *p

    Article Snippet: To elucidate the cellular mechanism by which the L. acidophilus CFF alters TNFα production, the THP-1 NF-κB-LUC2 (ATCC TIB-202-NFκB-LUC2™) cell line was used.

    Techniques: Concentration Assay, Derivative Assay, Incubation, Enzyme-linked Immunosorbent Assay

    L. acidophilus cell free filtrate (CFF) affects NF-κB activity. Using a luciferase NF-κB reporter THP-1 cell line, monocytes and monocyte derived macrophages were incubated with L. acidophilus CFF alone or added 1 h after LPS stimulation, for 20 h. Luciferase luminescence was measured to quantify NF-κB activation. (A) In monocytes, 10 8 CFF alone upregulated NF-κB activation but 10 6 CFF had no effect. (B) In LPS stimulated monocytes, only 10 6 CFF reduced NF-κB activity. (C) L. acidophilus 10 8 CFF increased NF-κB activity in monocyte derived macrophages. (D) Similar to monocytes, only L. acidophilus 10 6 CFF significantly reduced NF-κB activity in monocyte derived macrophages. Data are relative light units (RLUs) or mean ± s.e.m. percent change from LPS control adjusted to 100%. **p

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Different Concentrations of Lactobacillus acidophilus Cell Free Filtrate Have Differing Anti-Biofilm and Immunomodulatory Effects

    doi: 10.3389/fcimb.2021.737392

    Figure Lengend Snippet: L. acidophilus cell free filtrate (CFF) affects NF-κB activity. Using a luciferase NF-κB reporter THP-1 cell line, monocytes and monocyte derived macrophages were incubated with L. acidophilus CFF alone or added 1 h after LPS stimulation, for 20 h. Luciferase luminescence was measured to quantify NF-κB activation. (A) In monocytes, 10 8 CFF alone upregulated NF-κB activation but 10 6 CFF had no effect. (B) In LPS stimulated monocytes, only 10 6 CFF reduced NF-κB activity. (C) L. acidophilus 10 8 CFF increased NF-κB activity in monocyte derived macrophages. (D) Similar to monocytes, only L. acidophilus 10 6 CFF significantly reduced NF-κB activity in monocyte derived macrophages. Data are relative light units (RLUs) or mean ± s.e.m. percent change from LPS control adjusted to 100%. **p

    Article Snippet: To elucidate the cellular mechanism by which the L. acidophilus CFF alters TNFα production, the THP-1 NF-κB-LUC2 (ATCC TIB-202-NFκB-LUC2™) cell line was used.

    Techniques: Activity Assay, Luciferase, Derivative Assay, Incubation, Activation Assay